##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938143_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2049370 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48083118226577 33.0 31.0 34.0 31.0 34.0 2 32.70350058798557 34.0 31.0 34.0 31.0 34.0 3 32.75977007568179 34.0 31.0 34.0 31.0 34.0 4 36.2258542869272 37.0 35.0 37.0 35.0 37.0 5 36.1581120051528 37.0 35.0 37.0 35.0 37.0 6 36.218343198153576 37.0 37.0 37.0 35.0 37.0 7 36.188289571917224 37.0 37.0 37.0 35.0 37.0 8 36.187617658109566 37.0 37.0 37.0 35.0 37.0 9 37.864413453890705 39.0 38.0 39.0 35.0 39.0 10-11 37.84175990670303 39.0 38.0 39.0 35.0 39.0 12-13 37.753589883720366 39.0 38.0 39.0 35.0 39.0 14-15 38.971160893347715 40.0 38.0 41.0 35.5 41.0 16-17 38.88913129400743 40.0 38.0 41.0 35.5 41.0 18-19 39.15546167846704 40.0 38.5 41.0 36.0 41.0 20-21 39.147606581534816 40.0 39.0 41.0 36.0 41.0 22-23 39.038187101401896 40.0 39.0 41.0 35.0 41.0 24-25 38.928174268189736 40.0 38.0 41.0 35.0 41.0 26-27 38.77421695447869 40.0 38.0 41.0 35.0 41.0 28-29 38.64638254683146 40.0 38.0 41.0 34.5 41.0 30-31 38.38646632867662 40.0 38.0 41.0 34.0 41.0 32-33 38.1521474892284 40.0 37.0 41.0 34.0 41.0 34-35 38.042685801002264 40.0 37.0 41.0 34.0 41.0 36-37 37.98418245607186 40.0 37.0 41.0 33.0 41.0 38-39 37.81026217813279 40.0 37.0 41.0 33.0 41.0 40-41 37.58732854486989 40.0 37.0 41.0 33.0 41.0 42-43 37.320005172321245 40.0 36.5 41.0 32.5 41.0 44-45 37.00642563324338 40.0 36.0 41.0 31.5 41.0 46-47 36.569601145717954 39.0 35.5 41.0 30.5 41.0 48-49 36.115955635146406 39.0 35.0 41.0 29.5 41.0 50-51 35.73171438051694 39.0 35.0 40.0 28.5 41.0 52-53 35.212051752489785 38.0 35.0 40.0 27.0 41.0 54-55 34.832335059066935 38.0 34.0 40.0 26.0 41.0 56-57 34.480305898886 38.0 34.0 40.0 25.5 41.0 58-59 33.99468373207375 37.5 33.0 40.0 23.5 41.0 60-61 33.53505052772316 37.0 33.0 40.0 22.5 41.0 62-63 33.00257444970893 36.0 32.0 39.0 21.5 40.5 64-65 32.44201705890103 35.5 31.5 39.0 18.5 40.0 66-67 31.894434143175708 35.0 31.0 38.5 16.0 40.0 68-69 31.304438437178256 35.0 30.5 38.0 10.0 40.0 70-71 30.69784714326842 35.0 29.5 37.0 7.5 39.5 72-73 30.039567769607245 34.0 29.0 36.5 4.5 39.0 74-75 29.38918521301668 34.0 28.0 36.0 2.0 39.0 76-77 28.080281501144256 32.5 26.5 34.5 2.0 36.5 78-79 28.746529421236772 33.5 28.0 35.0 2.0 37.0 80-81 28.466143497757848 34.0 28.0 35.0 2.0 37.0 82-83 27.923624577309127 33.5 26.5 35.0 2.0 36.0 84-85 27.28374866422364 33.0 26.0 35.0 2.0 36.0 86-87 26.570597793468238 32.5 25.0 35.0 2.0 35.5 88-89 25.791710623264713 32.0 22.5 34.5 2.0 35.0 90-91 25.158970073729975 32.0 19.5 34.0 2.0 35.0 92-93 24.231910050405734 31.0 12.0 34.0 2.0 35.0 94-95 23.341262436748856 31.0 2.0 34.0 2.0 35.0 96-97 22.30562538731415 30.0 2.0 34.0 2.0 35.0 98-99 20.91071207249057 29.5 2.0 34.0 2.0 35.0 100-101 17.051849104846855 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19523504221997712 1101 2 0.21464943445235463 1101 3 0.19707626198051287 1101 4 0.055852470270949084 1101 5 0.03767199365936591 1101 6 0.10678229303023556 1101 7 0.19483425613065464 1101 8 0.21756723889677687 1101 9 0.1723901645371626 1101 10-11 0.21174806823880488 1101 12-13 0.3335282327549862 1101 14-15 0.4210035996444077 1101 16-17 0.4471269152428974 1101 18-19 0.34710712446661063 1101 20-21 0.2764443663315461 1101 22-23 0.40965381731529504 1101 24-25 0.4698737003532045 1101 26-27 0.4203327569221571 1101 28-29 0.4878997141605552 1101 30-31 0.518576167513487 1101 32-33 0.48093659192787896 1101 34-35 0.512265721123562 1101 36-37 0.5252109322884948 1101 38-39 0.5848552500017732 1101 40-41 0.7479560134593797 1101 42-43 0.8177320736957157 1101 44-45 0.7766229999436902 1101 46-47 0.7742819406518962 1101 48-49 0.8263499304015767 1101 50-51 0.8536158174264088 1101 52-53 0.9839387847198324 1101 54-55 0.922278456214535 1101 56-57 0.892414054575589 1101 58-59 1.0385465228290514 1101 60-61 1.092582229587471 1101 62-63 1.2104779536139176 1101 64-65 1.4595116133368222 1101 66-67 1.4264281938150276 1101 68-69 1.6635927349928217 1101 70-71 1.5859043834277493 1101 72-73 1.6034327365947654 1101 74-75 1.5960263869386182 1101 76-77 1.7446288678313735 1101 78-79 1.817426975767475 1101 80-81 2.07367207293402 1101 82-83 2.0328554683400775 1101 84-85 2.353991094618653 1101 86-87 2.643874281719633 1101 88-89 2.866352421364617 1101 90-91 3.1587333602626693 1101 92-93 3.4421560623861396 1101 94-95 3.8751085006298496 1101 96-97 4.161070539704294 1101 98-99 4.7254170605401775 1101 100-101 4.700816224164512 1102 1 0.16020023943575268 1102 2 0.1224299659857877 1102 3 0.1340212630351374 1102 4 0.06308327600812902 1102 5 -0.07474231766735784 1102 6 -0.056277979064255135 1102 7 0.03339284081743443 1102 8 0.0522896075953625 1102 9 0.08549130353737411 1102 10-11 0.07235105284120635 1102 12-13 0.1844292821116582 1102 14-15 0.19429773590258037 1102 16-17 0.14392611372422692 1102 18-19 0.16437075555709413 1102 20-21 0.07506214211724682 1102 22-23 0.1888222416983325 1102 24-25 0.22187222387145056 1102 26-27 0.22080799948701468 1102 28-29 0.21552640156827607 1102 30-31 0.19945704707475898 1102 32-33 0.028360658580488973 1102 34-35 0.191215968961572 1102 36-37 0.1852885794093595 1102 38-39 0.23639830526438033 1102 40-41 0.422554594982266 1102 42-43 0.6356924196143083 1102 44-45 0.4354621161638974 1102 46-47 0.47996969112647747 1102 48-49 0.4054240972440155 1102 50-51 0.5152114723515311 1102 52-53 0.5072480812817375 1102 54-55 0.5678911961428241 1102 56-57 0.6223312661343314 1102 58-59 0.7727119151513477 1102 60-61 0.5302760178178048 1102 62-63 0.6545245682521781 1102 64-65 0.752649928038359 1102 66-67 0.9085317848295773 1102 68-69 1.0582187368911526 1102 70-71 1.0538943908101608 1102 72-73 1.1996017937557006 1102 74-75 1.3049867855886923 1102 76-77 1.294748172830321 1102 78-79 1.288244050224339 1102 80-81 1.386855604889302 1102 82-83 1.5384311643957638 1102 84-85 1.8383990518006819 1102 86-87 1.873147509941525 1102 88-89 1.9326728179054307 1102 90-91 2.1883987789507096 1102 92-93 2.3946443296293367 1102 94-95 2.6165752270588705 1102 96-97 3.0187653825644496 1102 98-99 3.3861874676272876 1102 100-101 3.382147957975942 1103 1 0.21190634840658618 1103 2 0.15518813379485863 1103 3 0.18697349448558498 1103 4 0.06546138427850678 1103 5 0.024081605382626492 1103 6 0.011590197750670939 1103 7 0.094382796788814 1103 8 0.10889233686938837 1103 9 0.19604628927810097 1103 10-11 0.1809050504396481 1103 12-13 0.20814579159281976 1103 14-15 0.28866219780282165 1103 16-17 0.33065123505308947 1103 18-19 0.27028194157801266 1103 20-21 0.30387446387639017 1103 22-23 0.29983127256839737 1103 24-25 0.37097163849094983 1103 26-27 0.4231959579099893 1103 28-29 0.4279708967401419 1103 30-31 0.38834138439401755 1103 32-33 0.3354796609320161 1103 34-35 0.487730109402861 1103 36-37 0.41939715623429663 1103 38-39 0.4411058964486543 1103 40-41 0.5180027300200365 1103 42-43 0.5821751333782501 1103 44-45 0.5792859141325835 1103 46-47 0.7845471977557921 1103 48-49 0.7758906817739586 1103 50-51 0.8551136587727015 1103 52-53 0.9474434270064549 1103 54-55 1.0538658296546046 1103 56-57 1.1626068202445055 1103 58-59 1.2056747472422131 1103 60-61 1.1315921858270102 1103 62-63 1.2319961747964356 1103 64-65 1.304339463736568 1103 66-67 1.4964415824480284 1103 68-69 1.5698161189394888 1103 70-71 1.6659830296754272 1103 72-73 1.8071560790411318 1103 74-75 1.8714290355511665 1103 76-77 1.71186007681791 1103 78-79 1.7743972372959398 1103 80-81 1.7671592084816403 1103 82-83 1.8907581456835985 1103 84-85 2.0757072083524406 1103 86-87 2.380145027077667 1103 88-89 2.494307880696244 1103 90-91 2.561184527187063 1103 92-93 2.7600454340972433 1103 94-95 2.9657994102779632 1103 96-97 3.314016358198611 1103 98-99 3.3997031278064043 1103 100-101 3.4479479154482995 1104 1 0.21154794833063306 1104 2 0.16656436931830143 1104 3 0.13061982956295282 1104 4 0.10146694422670777 1104 5 0.04579952256787578 1104 6 0.06817527994088834 1104 7 0.04095685698993634 1104 8 0.11288903175610443 1104 9 0.24620865222642152 1104 10-11 0.2508259977076648 1104 12-13 0.26241032229788885 1104 14-15 0.4098055771179716 1104 16-17 0.32854385447404155 1104 18-19 0.30546745367031036 1104 20-21 0.27861289560903657 1104 22-23 0.3262916119507153 1104 24-25 0.3253885188971779 1104 26-27 0.3085338938756408 1104 28-29 0.2614488164175768 1104 30-31 0.3166204261485106 1104 32-33 0.5145558313322667 1104 34-35 0.4525512728086625 1104 36-37 0.5157245938666009 1104 38-39 0.5461500511599908 1104 40-41 0.5526797175012561 1104 42-43 0.6639575507096254 1104 44-45 0.6770864116797028 1104 46-47 0.8257175164144002 1104 48-49 1.0137239931028788 1104 50-51 1.1729853022951673 1104 52-53 1.1099748945627894 1104 54-55 1.1461624531876637 1104 56-57 1.2200677277858887 1104 58-59 1.2480878953032715 1104 60-61 1.3413195733189056 1104 62-63 1.3628497336528156 1104 64-65 1.3835752527136833 1104 66-67 1.5757663780665325 1104 68-69 1.90787951243637 1104 70-71 1.9193616476099642 1104 72-73 2.095941538916197 1104 74-75 2.033850728249803 1104 76-77 2.124121335045462 1104 78-79 2.054228184015077 1104 80-81 2.1718615776900876 1104 82-83 2.307324358465845 1104 84-85 2.2982273720653232 1104 86-87 2.3257211744845314 1104 88-89 2.427183967133562 1104 90-91 2.6200714694677387 1104 92-93 3.018484227938579 1104 94-95 3.2353895495288576 1104 96-97 3.400949589866581 1104 98-99 3.640147893993376 1104 100-101 3.6140963162214703 1105 1 0.13079929356081976 1105 2 0.17544494233891328 1105 3 0.20290263455379431 1105 4 0.10152586665447672 1105 5 -0.12157954835574714 1105 6 -0.06221268741948904 1105 7 0.06961525279851344 1105 8 0.13925426865684187 1105 9 0.16311216533453177 1105 10-11 0.11919485210552949 1105 12-13 0.199016447114289 1105 14-15 0.3019238525604919 1105 16-17 0.36104382260494106 1105 18-19 0.2816510366921463 1105 20-21 0.24479907065113338 1105 22-23 0.20111204726848797 1105 24-25 0.21107348503802825 1105 26-27 0.228212426707195 1105 28-29 0.3411651955777373 1105 30-31 0.34078981113150775 1105 32-33 0.3831885618910462 1105 34-35 0.2560034755301501 1105 36-37 0.26678654315082184 1105 38-39 0.35547509191703597 1105 40-41 0.35400986298015624 1105 42-43 0.30629147373937116 1105 44-45 0.4072599353966879 1105 46-47 0.40719151193572856 1105 48-49 0.5214662018157483 1105 50-51 0.5271005947897791 1105 52-53 0.6690830129685423 1105 54-55 0.6146988682395076 1105 56-57 0.5635490801122671 1105 58-59 0.8250454017514528 1105 60-61 0.8802136163464453 1105 62-63 0.852015372245873 1105 64-65 0.8751899963515513 1105 66-67 0.9599850822546969 1105 68-69 1.3239751084098152 1105 70-71 1.5218545195542568 1105 72-73 1.480704000797214 1105 74-75 1.3896984251052444 1105 76-77 1.4261344707809265 1105 78-79 1.7844704033509835 1105 80-81 1.8511701739907203 1105 82-83 1.9514353025985969 1105 84-85 2.1971171942074044 1105 86-87 2.3377018320644893 1105 88-89 2.3901609944687507 1105 90-91 2.3904186160500274 1105 92-93 2.567534174610948 1105 94-95 2.7188446700436337 1105 96-97 3.0791995654336013 1105 98-99 3.1794021286887713 1105 100-101 3.07180573516985 1106 1 0.1516525843676746 1106 2 0.12558232925539414 1106 3 0.1453169926459239 1106 4 0.1602830536636617 1106 5 -0.0159216029559488 1106 6 0.08094609602857616 1106 7 0.10622756093845709 1106 8 0.12673747125464985 1106 9 0.21123455297578175 1106 10-11 0.19141222049352535 1106 12-13 0.16077839158765528 1106 14-15 0.14272012163586112 1106 16-17 0.10351099430003075 1106 18-19 0.010782442395225189 1106 20-21 0.05424213226073249 1106 22-23 0.08343841095519622 1106 24-25 0.037805382996744186 1106 26-27 -0.02451603319991591 1106 28-29 0.11286958567867345 1106 30-31 0.19835764534185074 1106 32-33 0.2752827921705503 1106 34-35 0.14069320157514653 1106 36-37 0.23570561856032413 1106 38-39 0.11971946122642407 1106 40-41 0.23715612274093445 1106 42-43 0.11325854990358408 1106 44-45 0.2098922151874163 1106 46-47 0.34636974499344575 1106 48-49 0.4070451202578127 1106 50-51 0.4371559549994899 1106 52-53 0.31460794122764213 1106 54-55 0.3841853785111411 1106 56-57 0.5952837485989164 1106 58-59 0.6787655013155671 1106 60-61 0.5523841070306617 1106 62-63 0.5803749785579555 1106 64-65 0.8609399958878043 1106 66-67 1.0152260439869565 1106 68-69 0.88083956506701 1106 70-71 0.836045373022607 1106 72-73 1.0762951646812944 1106 74-75 1.3445626692360584 1106 76-77 1.3338789377411544 1106 78-79 1.4101211991722167 1106 80-81 1.2060343916219871 1106 82-83 1.626276285424506 1106 84-85 1.733796148715367 1106 86-87 1.8737785843008545 1106 88-89 1.8336997284633547 1106 90-91 1.9789998539976992 1106 92-93 1.8969068852154578 1106 94-95 2.4040096125170862 1106 96-97 2.717503233845793 1106 98-99 2.867178734303124 1106 100-101 3.1658139484403485 1107 1 0.1939259303262375 1107 2 0.1579503437656271 1107 3 0.11035012225166696 1107 4 0.05245531006414694 1107 5 0.045472044480220575 1107 6 0.10597485893704572 1107 7 0.11841714992694818 1107 8 0.1392039790478563 1107 9 0.19879385308608732 1107 10-11 0.24273988228198107 1107 12-13 0.20414612169479796 1107 14-15 0.306288291406446 1107 16-17 0.31856322175438123 1107 18-19 0.2606508220872925 1107 20-21 0.27706240963376416 1107 22-23 0.35261097343742165 1107 24-25 0.3487262296404978 1107 26-27 0.32772184753763156 1107 28-29 0.3973913987767972 1107 30-31 0.5235457462466186 1107 32-33 0.5669888804098377 1107 34-35 0.514427388427471 1107 36-37 0.5126360368393392 1107 38-39 0.6107228260608792 1107 40-41 0.6347623622661516 1107 42-43 0.5931549673020342 1107 44-45 0.6145917569938959 1107 46-47 0.7145668461464894 1107 48-49 0.7373902854825971 1107 50-51 0.7645950212488941 1107 52-53 0.7962412305326936 1107 54-55 0.7934122717918441 1107 56-57 0.9693571803429464 1107 58-59 0.9262434239444985 1107 60-61 1.0177847411824672 1107 62-63 1.1341398097149096 1107 64-65 1.3047918048411873 1107 66-67 1.4428835225238146 1107 68-69 1.6882942812291084 1107 70-71 1.6993945885397643 1107 72-73 1.7711973553077591 1107 74-75 1.7086325704588887 1107 76-77 1.6132501384951574 1107 78-79 1.6759330564586357 1107 80-81 1.9046980841858492 1107 82-83 2.228352479646528 1107 84-85 2.33190450681138 1107 86-87 2.3931749013065584 1107 88-89 2.5721908229300894 1107 90-91 2.608061824451781 1107 92-93 2.7331012827325303 1107 94-95 2.801551848441587 1107 96-97 3.2457525650339747 1107 98-99 3.5437791211119816 1107 100-101 3.404260524273514 1108 1 0.13480591766919048 1108 2 0.1254375656416471 1108 3 0.049893909053686514 1108 4 0.03387657970758795 1108 5 -0.0432246568318746 1108 6 0.072994437530717 1108 7 0.04714809844098511 1108 8 -0.01619512139804158 1108 9 0.1347444820097934 1108 10-11 0.01954838101251255 1108 12-13 0.074127028503014 1108 14-15 0.19034854366474718 1108 16-17 0.1592903236467933 1108 18-19 0.11969081339096022 1108 20-21 0.15090366061452443 1108 22-23 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54-55 0.13893259605746522 2316 56-57 0.15237287945059563 2316 58-59 0.3199657030301353 2316 60-61 0.48527228328942584 2316 62-63 0.4945409290431826 2316 64-65 0.756289314864361 2316 66-67 0.8787618139944655 2316 68-69 1.116043907612454 2316 70-71 1.0958931637508407 2316 72-73 1.2068307736959447 2316 74-75 1.6688712286104597 2316 76-77 1.451557018327545 2316 78-79 1.4597593795741766 2316 80-81 1.5096713503723045 2316 82-83 1.5894826119700944 2316 84-85 1.8243034300906231 2316 86-87 2.1006358617696677 2316 88-89 2.446124233469522 2316 90-91 2.6466014037906582 2316 92-93 2.738687312272674 2316 94-95 3.2296398521833574 2316 96-97 3.6777166914277295 2316 98-99 3.844888918758315 2316 100-101 4.400234634601354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 15.0 5 42.0 6 103.0 7 235.0 8 509.0 9 961.0 10 1691.0 11 2792.0 12 4291.0 13 6747.0 14 8704.0 15 12208.0 16 17213.0 17 24680.0 18 25657.0 19 18914.0 20 17319.0 21 18889.0 22 20161.0 23 22359.0 24 25141.0 25 28553.0 26 32497.0 27 38159.0 28 45722.0 29 54652.0 30 65607.0 31 78923.0 32 95540.0 33 119987.0 34 158635.0 35 222658.0 36 296560.0 37 371853.0 38 203330.0 39 8057.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67776438612842 19.004425750352546 15.18647194015722 22.131337923361812 2 15.767055090380863 20.185393140531886 40.11417968610835 23.933372082978902 3 19.141977965352375 24.77990238703803 30.160863272561894 25.9172563750477 4 12.995545379887016 17.17227019718123 36.226957746740034 33.60522667619172 5 13.777864627860964 37.445809619189525 33.403111947043165 15.37321380590635 6 31.7945514963135 35.87624489477254 17.11530860703533 15.213895001878628 7 28.74146688982468 33.532646618229016 19.75699849221956 17.968887999726746 8 26.9366045985088 34.700296677987275 19.032868798063785 19.330229925440136 9 26.98980662349893 16.814630837769656 19.90826449103871 36.28729804769271 10-11 25.534090964540322 26.838979784031192 26.078258196421338 21.548671055007148 12-13 26.39508727072222 24.335795878733464 27.232368971927954 22.036747878616357 14-15 23.125405155097702 25.289241398391066 26.181662213671082 25.40369123284015 16-17 22.000370845674524 28.954825141384916 26.637820403343465 22.406983609597095 18-19 21.804505774945472 26.53954629959451 29.944885501397987 21.71106242406203 20-21 22.725300946144426 25.649589873961265 30.142677993724902 21.48243118616941 22-23 22.54441679632062 25.757630837447003 30.08026142725639 21.61769093897599 24-25 22.28254995519336 25.97955509557489 30.118087999603777 21.61980694962797 26-27 21.945051303960412 26.395566783473228 29.97979267607723 21.67958923648913 28-29 21.61127566032488 26.353684302980916 30.37635956415874 21.658680472535462 30-31 21.828781527981768 25.916013213816928 30.34434972698927 21.910855531212032 32-33 21.629354386957942 26.16384547446288 30.163830835817834 22.042969302761335 34-35 22.25680301530418 26.19802927706731 29.783156060340243 21.762011647288272 36-37 22.305598028258153 25.89960599808086 29.786608521557145 22.008187452103844 38-39 21.901276522650605 26.051036506722742 30.034710446170504 22.01297652445615 40-41 21.972195958104834 25.98903210457931 30.090317385761256 21.9484545515546 42-43 22.134328620407974 25.863971252033664 29.80661780995853 22.195082317599834 44-45 21.951608700362176 26.249674278068447 29.542442421633986 22.25627459993539 46-47 22.143439203267345 26.539839072495454 29.09213563192591 22.224586092311295 48-49 22.33959704689734 26.5425228240874 28.867115259811555 22.250764869203707 50-51 22.22004811234672 26.65926601833734 28.72526678930598 22.395419080009955 52-53 22.517700561636016 26.791379789886648 28.4893406266316 22.20157902184574 54-55 22.15798025734738 26.69032434357876 28.503832885228142 22.647862513845716 56-57 22.227568039828835 27.0987247541256 28.425571864907717 22.248135341137846 58-59 22.49825556146523 26.628158897612437 28.370328442399373 22.503257098522962 60-61 22.300877830747986 26.672733571780594 28.79267774974748 22.233710847723934 62-63 22.249203413732026 26.741120441891898 28.665687503964634 22.343988640411443 64-65 22.096497948149917 26.663706407334935 28.57819720206698 22.661598442448167 66-67 22.035622689531603 26.949454661144312 28.421981801218717 22.592940848105368 68-69 21.93896932509949 26.819842325346155 28.565325580618016 22.67586276893634 70-71 22.077980062165445 26.934033385869803 28.333878216232307 22.654108335732445 72-73 22.22032190876267 26.935333037795285 28.09754414711452 22.746800906327532 74-75 22.099108506516636 27.20206697668064 27.851803237092376 22.84702127971035 76-77 22.034600877342793 27.158834178308457 28.102538829005987 22.704026115342764 78-79 22.149807013862794 27.143341612300365 27.97608533354153 22.73076604029531 80-81 21.957121394069485 27.49278191753844 28.041975888164494 22.508120800227584 82-83 22.190789115735885 27.30710665533611 27.929042994878074 22.573061234049934 84-85 22.118494303951426 27.081550687646804 27.808882327207495 22.991072681194268 86-87 22.26939522400805 26.856909329348255 28.03001006435085 22.84368538229284 88-89 22.209776663072066 27.112600457701635 27.735669986386057 22.941952892840238 90-91 22.06987785368158 27.170120399384277 27.95475245382031 22.805249293113835 92-93 22.144659090354597 26.933667419743628 27.77675578348468 23.144917706417097 94-95 21.97797371875259 26.864353435446013 27.82869857565984 23.328974270141558 96-97 22.13662403808783 26.919097273135602 27.585058519246296 23.359220169530275 98-99 22.03191712575084 27.032527069294467 27.43279642036333 23.50275938459136 100-101 22.30551340167954 26.534227591894094 27.631589220101787 23.528669786324578 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2740.0 1 1774.0 2 1165.0 3 1515.5 4 1161.5 5 1010.0 6 2153.5 7 5052.0 8 7817.0 9 8546.0 10 6588.5 11 4153.0 12 3613.0 13 3793.5 14 3927.0 15 3945.0 16 4007.0 17 4049.5 18 4178.0 19 4311.0 20 4611.0 21 5136.5 22 5875.0 23 6931.0 24 7990.5 25 9483.0 26 11392.5 27 13442.5 28 15201.0 29 17326.5 30 20264.0 31 23099.5 32 26137.5 33 29418.0 34 32832.5 35 35636.5 36 39253.0 37 43287.0 38 46620.5 39 49123.0 40 52210.5 41 58259.0 42 63604.5 43 67914.5 44 72995.5 45 78345.0 46 79886.0 47 80309.5 48 83881.5 49 83604.0 50 80195.5 51 76123.0 52 71759.5 53 70600.0 54 70096.0 55 65441.5 56 58294.0 57 52664.0 58 47780.5 59 42144.0 60 36010.0 61 29970.5 62 24863.0 63 19889.5 64 15813.0 65 12685.5 66 9379.0 67 7572.0 68 5829.0 69 3657.5 70 2559.5 71 1923.0 72 1577.5 73 1307.0 74 1011.5 75 822.0 76 558.5 77 291.5 78 149.0 79 93.5 80 52.0 81 21.0 82 14.0 83 9.0 84 4.0 85 2.0 86 1.0 87 1.5 88 1.0 89 1.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015565759233325364 3 0.0025373651414825043 4 0.001805432889131782 5 0.11808507004591655 6 0.0 7 0.0 8 4.8795483490048157E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.4397741745024082E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.1957967570521673E-4 24-25 4.147616096654094E-4 26-27 0.005513889634375442 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010905790560025764 36-37 7.075345106056984E-4 38-39 0.006392208337196309 40-41 0.010125062824184993 42-43 0.005465094150885394 44-45 0.00370845674524366 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.4397741745024082E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.759096698009633E-5 68-69 0.0011466938620161317 70-71 0.0 72-73 2.4397741745024082E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4638645047014448E-4 82-83 0.002171399015307143 84-85 0.004806355123769744 86-87 0.0028057403006777693 88-89 0.0 90-91 0.0031961041685981545 92-93 0.0 94-95 0.0 96-97 7.319322523507224E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2049370.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.19494091370118 #Duplication Level Percentage of deduplicated Percentage of total 1 73.56375623512434 35.45400885141735 2 14.179569896062025 13.667670666448103 3 5.053681693348327 7.306856718227278 4 2.2682975494734237 4.372818654862593 5 1.278247419712014 3.080252943305575 6 0.7670043268077805 2.217943692663248 7 0.5164424287529021 1.7422938637352114 8 0.3598759639624934 1.3875360655546898 9 0.2782617786808834 1.2069728983859913 >10 1.5100077859113037 13.321455857279252 >50 0.13515967230088127 4.540684429827795 >100 0.08422971503402187 7.454293595553084 >500 0.0034412626704562774 1.0449617014254453 >1k 0.001720631335228138 1.3297383988823466 >5k 1.0121360795459634E-4 0.43570151793166245 >10k+ 2.0242721590919268E-4 1.4368101445003492 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15747 0.7683824785177884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13708 0.6688884876815803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8932 0.4358412585331102 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3458 0.16873478190858654 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2596 0.12667307514016504 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2355 0.11491336361906342 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.879548349004816E-5 0.0 14-15 0.0 0.0 0.0 4.879548349004816E-5 0.0 16-17 0.0 0.0 0.0 4.879548349004816E-5 0.0 18-19 0.0 0.0 0.0 7.319322523507224E-5 0.0 20-21 0.0 0.0 0.0 2.1957967570521673E-4 0.0 22-23 0.0 0.0 0.0 4.879548349004816E-4 0.0 24-25 0.0 0.0 0.0 8.051254775857947E-4 0.0 26-27 0.0 0.0 0.0 0.0020006148230919747 0.0 28-29 0.0 0.0 0.0 0.004635570931554575 0.0 30-31 0.0 0.0 0.0 0.013418757959763244 0.0 32-33 0.0 0.0 0.0 0.03213182587819671 0.0 34-35 0.0 0.0 0.0 0.059530489857858755 0.0 36-37 0.0 0.0 0.0 0.10439793692695803 0.0 38-39 0.0 0.0 0.0 0.16719772417865003 0.0 40-41 0.0 0.0 0.0 0.2337303659173307 0.0 42-43 0.0 0.0 0.0 0.30094614442487205 0.0 44-45 0.0 0.0 0.0 0.3807267599311008 0.0 46-47 0.0 0.0 0.0 0.47668307821428046 0.0 48-49 0.0 0.0 0.0 0.5780312974231105 0.0 50-51 0.0 0.0 0.0 0.6846494288488657 0.0 52-53 0.0 0.0 0.0 0.7879494673972978 0.0 54-55 0.0 0.0 0.0 0.8979588849256113 0.0 56-57 0.0 0.0 0.0 1.0285599964867251 0.0 58-59 0.0 0.0 0.0 1.1689446024875938 0.0 60-61 0.0 0.0 0.0 1.3144039387714272 0.0 62-63 0.0 0.0 0.0 1.4593509224786154 0.0 64-65 0.0 0.0 0.0 1.6071524419699712 0.0 66-67 0.0 0.0 0.0 1.7787173619209806 0.0 68-69 0.0 0.0 0.0 1.9737041139472131 0.0 70-71 0.0 0.0 0.0 2.182158419416699 0.0 72-73 0.0 0.0 0.0 2.3805852530289795 0.0 74-75 0.0 0.0 0.0 2.579548836959651 0.0 76-77 0.0 0.0 0.0 2.7915164172404205 0.0 78-79 0.0 0.0 0.0 3.0246368396141254 0.0 80-81 0.0 0.0 0.0 3.2839116411384963 0.0 82-83 0.0 0.0 0.0 3.5455286258703893 0.0 84-85 0.0 0.0 0.0 3.8078287473711434 0.0 86-87 0.0 0.0 0.0 4.076643065917819 0.0 88-89 0.0 0.0 0.0 4.370099103626968 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6820 0.0 52.866146 1 GTATCAA 11550 0.0 39.57621 1 TATCAAC 14730 0.0 30.935389 2 ATCAACG 14790 0.0 30.706657 3 TCAACGC 14905 0.0 30.563961 4 CAACGCA 15185 0.0 29.905706 5 AACGCAG 15570 0.0 29.161861 6 GTGGTAT 3110 0.0 26.918142 1 ACGCAGA 17735 0.0 25.468025 7 CGCAGAG 17760 0.0 25.405436 8 GTACATG 11220 0.0 25.054596 1 TGGTATC 3345 0.0 24.458237 2 GAGTACT 10415 0.0 24.351625 12-13 TACATGG 11535 0.0 24.246695 2 GCAGAGT 18880 0.0 23.546146 9 GTACTTT 10920 0.0 23.160234 14-15 ACATGGG 11775 0.0 22.858768 3 AGTACTT 10720 0.0 21.42139 12-13 CAGAGTA 18435 0.0 21.332079 10-11 AGAGTAC 17495 0.0 20.387875 10-11 >>END_MODULE