FastQCFastQC Report
Thu 26 May 2016
SRR938142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029042
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163780.8071789544031124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135350.6670635699014609No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86290.42527458771183646No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.15963198396090372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25090.12365441425066608No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.1195145295168853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66600.054.0051161
GTATCAA114950.041.303951
TATCAAC142550.032.974932
ATCAACG144750.032.4417233
TCAACGC145800.032.1754884
CAACGCA148350.031.5583445
AACGCAG153550.030.5462446
GTGGTAT28700.027.2945611
ACGCAGA170750.027.210057
CGCAGAG171300.027.1781548
TGGTATC30650.025.4312762
GCAGAGT179650.025.2267729
GAGTACT102350.024.90132112-13
GTACATG113600.024.5554941
GTACTTT108650.023.43672814-15
TACATGG116800.023.3573632
GTATAGG4150.023.0195081
ACATGGG117250.022.61973
CAGAGTA176850.022.23994310-11
AGTACTT104800.021.59942412-13
Produced by FastQC (version 0.11.5)