##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938142_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2029042 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78415380263198 33.0 31.0 34.0 30.0 34.0 2 31.975218847120956 33.0 31.0 34.0 30.0 34.0 3 32.03998290819017 34.0 31.0 34.0 30.0 34.0 4 35.574527289233046 37.0 35.0 37.0 33.0 37.0 5 35.58296082584786 37.0 35.0 37.0 33.0 37.0 6 35.61467234290863 37.0 35.0 37.0 33.0 37.0 7 35.55655969664502 37.0 35.0 37.0 33.0 37.0 8 35.532680940069255 37.0 35.0 37.0 33.0 37.0 9 37.14382403124233 39.0 37.0 39.0 34.0 39.0 10-11 37.04660007037804 39.0 37.0 39.0 33.0 39.0 12-13 36.9284731415121 39.0 37.0 39.0 33.0 39.0 14-15 38.065197763279414 40.0 38.0 41.0 33.0 41.0 16-17 37.91666461315241 40.0 38.0 41.0 33.0 41.0 18-19 38.089082187554524 40.0 38.0 41.0 33.0 41.0 20-21 38.09318239839294 40.0 38.0 41.0 33.0 41.0 22-23 37.993671644056654 40.0 38.0 41.0 33.0 41.0 24-25 37.87095166093161 40.0 38.0 41.0 33.0 41.0 26-27 37.690975346986406 40.0 38.0 41.0 32.0 41.0 28-29 37.4716321791269 40.0 37.0 41.0 32.0 41.0 30-31 37.228173443427984 40.0 37.0 41.0 31.5 41.0 32-33 36.953515008560686 39.5 36.0 41.0 31.0 41.0 34-35 36.699889898779816 39.0 35.5 41.0 30.0 41.0 36-37 36.42510652810538 39.0 35.0 41.0 30.0 41.0 38-39 36.13814844640969 39.0 35.0 40.0 30.0 41.0 40-41 35.903136307676235 39.0 35.0 40.0 29.0 41.0 42-43 35.693293928859035 39.0 35.0 40.0 27.5 41.0 44-45 35.362954783587526 38.5 35.0 40.0 26.5 41.0 46-47 34.8942449195236 38.0 34.5 40.0 24.5 41.0 48-49 34.450460611460976 38.0 34.0 40.0 23.0 41.0 50-51 33.74682608837077 37.5 33.0 39.5 22.0 40.5 52-53 33.8840965342265 38.0 33.5 39.5 22.5 40.5 54-55 33.97568359846667 38.0 34.0 40.0 21.5 41.0 56-57 33.67540987323082 38.0 33.0 40.0 19.5 41.0 58-59 33.34935600150219 37.5 33.0 40.0 18.5 41.0 60-61 32.93273894773987 37.0 32.5 40.0 15.0 41.0 62-63 32.387447869487175 36.0 31.5 39.0 10.0 41.0 64-65 31.85293133409757 35.5 31.0 39.0 7.5 40.5 66-67 31.309170781087822 35.0 30.5 38.5 3.5 40.0 68-69 30.69182698041736 35.0 29.5 38.0 2.0 40.0 70-71 30.129447788660855 35.0 29.0 37.5 2.0 39.5 72-73 29.46750215126153 34.0 28.5 37.0 2.0 39.0 74-75 28.7561819814474 34.0 27.5 36.0 2.0 39.0 76-77 28.06433775151032 34.0 26.0 35.5 2.0 38.0 78-79 27.307030608533488 33.0 25.5 35.0 2.0 37.0 80-81 26.596829439706028 32.5 23.5 35.0 2.0 36.5 82-83 25.848374750251597 32.0 21.0 35.0 2.0 36.0 84-85 25.155864196009745 31.5 19.0 35.0 2.0 35.5 86-87 24.418340034361044 31.0 14.0 34.0 2.0 35.0 88-89 23.57554722869216 30.5 4.5 34.0 2.0 35.0 90-91 22.712036271304388 30.0 2.0 34.0 2.0 35.0 92-93 21.776722709534845 29.0 2.0 34.0 2.0 35.0 94-95 20.782716424795545 29.0 2.0 34.0 2.0 35.0 96-97 19.55192721491226 26.5 2.0 33.5 2.0 35.0 98-99 18.03350201720812 24.5 2.0 33.0 2.0 35.0 100-101 14.514461997336674 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43374315761627 1101 2 0.33407817836464915 1101 3 0.2629258600914781 1101 4 0.2526143713239577 1101 5 0.30087730007347346 1101 6 0.29521783682120173 1101 7 0.29885072563449455 1101 8 0.3470545154345501 1101 9 0.30964782527329504 1101 10-11 0.3738092825179393 1101 12-13 0.4821179386613821 1101 14-15 0.6004616290985965 1101 16-17 0.7441653849728169 1101 18-19 0.5521836530695552 1101 20-21 0.5952341221854667 1101 22-23 0.5786357503018493 1101 24-25 0.6195010844886113 1101 26-27 0.6527033245839888 1101 28-29 0.6561719523133789 1101 30-31 0.8487841165289112 1101 32-33 0.7443128854582071 1101 34-35 0.8240541122769045 1101 36-37 0.8056283531442503 1101 38-39 0.9827196529816362 1101 40-41 0.95805334678316 1101 42-43 1.0516843544756327 1101 44-45 0.995265241551472 1101 46-47 1.0956331965478796 1101 48-49 1.1462624522650415 1101 50-51 1.1163671762540872 1101 52-53 1.0693633058850693 1101 54-55 1.1486463327507366 1101 56-57 1.1820885341107115 1101 58-59 1.2599864227887565 1101 60-61 1.3090879655923189 1101 62-63 1.2027732752318983 1101 64-65 1.318552812502201 1101 66-67 1.3374594472763697 1101 68-69 1.5368275655683021 1101 70-71 1.8681605901005547 1101 72-73 2.1515682077041376 1101 74-75 2.330676836661791 1101 76-77 2.252522155558861 1101 78-79 2.417048187672865 1101 80-81 2.6639721970503913 1101 82-83 2.5797174314205797 1101 84-85 2.6248598200155975 1101 86-87 3.0335920515441295 1101 88-89 3.439774329817819 1101 90-91 3.5849672002579247 1101 92-93 3.8024121361992513 1101 94-95 4.288790903906836 1101 96-97 4.470749723273297 1101 98-99 4.906127328546599 1101 100-101 4.902805047631093 1102 1 0.4783863220580926 1102 2 0.33309359248073633 1102 3 0.2462038796111088 1102 4 0.19321102299443993 1102 5 0.2416091632885582 1102 6 0.4041098396729339 1102 7 0.18439292392837103 1102 8 0.27238209613125974 1102 9 0.4947352210989422 1102 10-11 0.38798928595969073 1102 12-13 0.44407063920468914 1102 14-15 0.5992816010729314 1102 16-17 0.735715278032778 1102 18-19 0.5312731876960015 1102 20-21 0.624915514618543 1102 22-23 0.6003691623037213 1102 24-25 0.5854880058446597 1102 26-27 0.6321671493498258 1102 28-29 0.5956279102257085 1102 30-31 0.6803289698858421 1102 32-33 0.6934936264174709 1102 34-35 0.6668940463920094 1102 36-37 0.8840467468310962 1102 38-39 0.8083065940048186 1102 40-41 0.855323917375884 1102 42-43 1.0371312900903646 1102 44-45 1.0152501224423958 1102 46-47 1.0636943859177919 1102 48-49 1.2787285879190975 1102 50-51 1.3459723585438326 1102 52-53 1.1675084493322316 1102 54-55 1.2124588066303303 1102 56-57 1.3636714925393036 1102 58-59 1.3859716232706063 1102 60-61 1.5158909500798643 1102 62-63 1.4720581290805157 1102 64-65 1.5479355103412864 1102 66-67 1.5659306963852053 1102 68-69 1.7457896325840636 1102 70-71 1.852200711053424 1102 72-73 1.9231141673373457 1102 74-75 1.8656996997047877 1102 76-77 2.33902681421117 1102 78-79 2.693867397545766 1102 80-81 2.7596233020974665 1102 82-83 2.908128449932267 1102 84-85 2.9607283698103224 1102 86-87 3.1021406170725605 1102 88-89 3.530449650023339 1102 90-91 3.7452162353391714 1102 92-93 4.106684821008848 1102 94-95 4.304091319375036 1102 96-97 4.390310531592494 1102 98-99 4.825519737032966 1102 100-101 4.609100988826361 1103 1 0.6026984549380003 1103 2 0.44951527821188364 1103 3 0.3825153941834927 1103 4 0.2614370424992316 1103 5 0.38340675188921836 1103 6 0.2649850258678157 1103 7 0.42109650168053747 1103 8 0.3913810000406457 1103 9 0.4483860459773865 1103 10-11 0.5463455716177634 1103 12-13 0.5385262475349748 1103 14-15 0.6334114737577892 1103 16-17 0.7800697087682806 1103 18-19 0.7404185180689566 1103 20-21 0.7131107592715864 1103 22-23 0.7654108980615675 1103 24-25 0.8805794872532786 1103 26-27 0.78354147834564 1103 28-29 0.9130972056361841 1103 30-31 0.7673381882889245 1103 32-33 0.8098099188207115 1103 34-35 1.02368278195312 1103 36-37 0.9277189314240033 1103 38-39 1.0319666934961234 1103 40-41 1.0992125096020686 1103 42-43 1.144335275363396 1103 44-45 1.0610533601254275 1103 46-47 1.119546526104969 1103 48-49 1.0956751324231888 1103 50-51 1.1178569317711151 1103 52-53 1.1736669327925071 1103 54-55 1.3459155672610024 1103 56-57 1.443362900875215 1103 58-59 1.6429458510888182 1103 60-61 1.5995802786541091 1103 62-63 1.6841102074369374 1103 64-65 1.7889264886724128 1103 66-67 1.7673036201366408 1103 68-69 1.9433641807979996 1103 70-71 2.296439911750422 1103 72-73 2.3772743933006417 1103 74-75 2.6163601645596266 1103 76-77 2.737771930986977 1103 78-79 2.83381498193069 1103 80-81 3.0401901776771325 1103 82-83 2.9186200044135937 1103 84-85 2.9698308402299283 1103 86-87 3.263893378377748 1103 88-89 3.6688641127984134 1103 90-91 3.765867004007845 1103 92-93 4.109359703670947 1103 94-95 4.1459345784177835 1103 96-97 4.563329839650162 1103 98-99 5.263506302896769 1103 100-101 4.875816370631258 1104 1 0.3173716610434312 1104 2 0.2764367358238786 1104 3 0.27494675835398397 1104 4 0.2736036033870164 1104 5 0.3250419467213419 1104 6 0.21603035159344586 1104 7 0.20325244278788546 1104 8 0.2929507166998775 1104 9 0.2826851090488276 1104 10-11 0.3332453812157823 1104 12-13 0.2902447353787849 1104 14-15 0.490902817694149 1104 16-17 0.5250597173772178 1104 18-19 0.464029620452429 1104 20-21 0.3631084028114273 1104 22-23 0.3611455480801098 1104 24-25 0.5315520769087314 1104 26-27 0.57726056944324 1104 28-29 0.7281318927296923 1104 30-31 0.5334535480104208 1104 32-33 0.55822889115273 1104 34-35 0.609715414213376 1104 36-37 0.7708572336415855 1104 38-39 0.6830014936997202 1104 40-41 0.6278554399074068 1104 42-43 0.7111025890616602 1104 44-45 0.7511237083159799 1104 46-47 0.8646400618634686 1104 48-49 0.9373915465820559 1104 50-51 0.9128001253716036 1104 52-53 0.9175691993929789 1104 54-55 0.9777657969373195 1104 56-57 1.0997145035823195 1104 58-59 1.0792337665327452 1104 60-61 1.1209386051275203 1104 62-63 1.1589820160043942 1104 64-65 1.3316470690528455 1104 66-67 1.4434248238793295 1104 68-69 1.4574327192271497 1104 70-71 1.6811427149954277 1104 72-73 1.6805189247421026 1104 74-75 1.7847259437310328 1104 76-77 1.826359751544107 1104 78-79 2.207923611601977 1104 80-81 2.2143253567995274 1104 82-83 2.406166897970717 1104 84-85 2.5509631350450874 1104 86-87 2.6484004383323807 1104 88-89 2.9157292353029227 1104 90-91 3.2330837732067117 1104 92-93 3.212435826759858 1104 94-95 3.254187844471563 1104 96-97 3.636165352447314 1104 98-99 3.867263128776358 1104 100-101 3.6889272436526195 1105 1 0.3319494015256623 1105 2 0.2645577867524551 1105 3 0.12982028732302808 1105 4 0.04784596836744015 1105 5 0.14356629925438114 1105 6 0.25298257812599445 1105 7 0.21724704520506322 1105 8 0.12929064826412429 1105 9 0.2315730576473456 1105 10-11 0.168598956077318 1105 12-13 0.13847143859825195 1105 14-15 0.2836490305561341 1105 16-17 0.34505222666061286 1105 18-19 0.25262144869693515 1105 20-21 0.3423810447468796 1105 22-23 0.36774152703498686 1105 24-25 0.30664556271133137 1105 26-27 0.3935512187295558 1105 28-29 0.49590912924788455 1105 30-31 0.40017876553158516 1105 32-33 0.392084085644683 1105 34-35 0.40915230670380964 1105 36-37 0.6562391950003885 1105 38-39 0.46927214798773775 1105 40-41 0.6604433758238457 1105 42-43 0.7007712078185548 1105 44-45 0.642193873336943 1105 46-47 0.7138169719665655 1105 48-49 0.7932517878258025 1105 50-51 0.7001408143041772 1105 52-53 0.6731513833398566 1105 54-55 0.8195570026937915 1105 56-57 1.0002277124891563 1105 58-59 1.0185869336717275 1105 60-61 1.1056570691513414 1105 62-63 1.0509409924785658 1105 64-65 1.1716160868800323 1105 66-67 1.341025187036859 1105 68-69 1.335724456563753 1105 70-71 1.4140724741191129 1105 72-73 1.6121686246986044 1105 74-75 1.6876603608477971 1105 76-77 1.8326139072917513 1105 78-79 2.111989458793733 1105 80-81 2.248484802391676 1105 82-83 2.420652900879297 1105 84-85 2.585055140482968 1105 86-87 2.7995583585206916 1105 88-89 3.094641801335605 1105 90-91 3.1599517410298184 1105 92-93 3.3407056964190183 1105 94-95 3.8189679646786026 1105 96-97 3.84782538769635 1105 98-99 4.059367534194802 1105 100-101 3.7814788401115944 1106 1 0.35492747358713217 1106 2 0.21980610439925385 1106 3 0.2456368509054414 1106 4 0.24873083038045962 1106 5 0.3493094610518739 1106 6 0.14386736765150232 1106 7 0.2725218091003967 1106 8 0.3037234159844573 1106 9 0.3900562355183581 1106 10-11 0.40654516052622114 1106 12-13 0.4569471713008042 1106 14-15 0.6240528187064811 1106 16-17 0.3929660011342051 1106 18-19 0.45680998729969247 1106 20-21 0.5276222843393654 1106 22-23 0.39923337371034506 1106 24-25 0.37700614596919735 1106 26-27 0.5320756630368209 1106 28-29 0.46925827888758675 1106 30-31 0.540089241122125 1106 32-33 0.5612430364838872 1106 34-35 0.7058007649763809 1106 36-37 0.809535073337365 1106 38-39 0.6648916110236769 1106 40-41 0.9109196304364531 1106 42-43 0.9759101675386503 1106 44-45 0.9391263189555303 1106 46-47 1.3176418804978596 1106 48-49 1.4982195671289347 1106 50-51 1.4693784725461683 1106 52-53 1.3954758285356732 1106 54-55 1.059518593668301 1106 56-57 1.3233462700158256 1106 58-59 1.4723227176439906 1106 60-61 1.787583992858707 1106 62-63 1.5861813785891243 1106 64-65 1.5504668980035454 1106 66-67 1.8067602255569533 1106 68-69 1.597820575465704 1106 70-71 1.8737362162656162 1106 72-73 2.0963884451680954 1106 74-75 2.05447777814598 1106 76-77 2.106929899976592 1106 78-79 2.3905472567074497 1106 80-81 2.6902897272864337 1106 82-83 2.7868825847940144 1106 84-85 3.01054979172336 1106 86-87 2.731567649080784 1106 88-89 2.8311794098418055 1106 90-91 3.126406276514672 1106 92-93 3.467318806509539 1106 94-95 3.61502027166366 1106 96-97 3.76914230331084 1106 98-99 3.885153631979513 1106 100-101 3.8175093400485753 1107 1 0.16531903304143825 1107 2 0.07137275354470773 1107 3 -0.012972691590782404 1107 4 -0.05555432323926368 1107 5 0.06541868709808085 1107 6 0.10137830023253258 1107 7 0.12198636658510509 1107 8 0.12454203183549595 1107 9 0.06621685713754033 1107 10-11 0.11501334019892795 1107 12-13 0.1599938861405903 1107 14-15 0.31505170766582324 1107 16-17 0.3935394744645748 1107 18-19 0.26507070756947115 1107 20-21 0.3747692005481795 1107 22-23 0.3296233469376517 1107 24-25 0.32332474716240966 1107 26-27 0.35034654379504104 1107 28-29 0.5500039373865491 1107 30-31 0.573497158940107 1107 32-33 0.446772555645758 1107 34-35 0.38668882030071217 1107 36-37 0.3904181063670151 1107 38-39 0.4993341669944158 1107 40-41 0.5219552935009233 1107 42-43 0.6022386042483063 1107 44-45 0.69590704170691 1107 46-47 0.7273449992519474 1107 48-49 0.7847702256063016 1107 50-51 0.6759384074972203 1107 52-53 0.6999061969198479 1107 54-55 0.7155979904657173 1107 56-57 0.6052152862982894 1107 58-59 0.6070344095232585 1107 60-61 0.7437764026488622 1107 62-63 0.9144170828318678 1107 64-65 1.0713276011384316 1107 66-67 1.0593241524368828 1107 68-69 1.1302740300431466 1107 70-71 1.2672286858915278 1107 72-73 1.3947879693908938 1107 74-75 1.713227172232262 1107 76-77 1.8000384935772793 1107 78-79 2.044718749662941 1107 80-81 2.1194827581594566 1107 82-83 2.184510834542504 1107 84-85 2.3812235450523325 1107 86-87 2.562844341383876 1107 88-89 2.9233513568490928 1107 90-91 3.0768414549390783 1107 92-93 3.427570639363026 1107 94-95 3.7446428564455623 1107 96-97 3.7350448994281287 1107 98-99 3.776629457130028 1107 100-101 3.9748752169423636 1108 1 0.2889548532054036 1108 2 0.15117069222986146 1108 3 0.13897621981109864 1108 4 0.1296902993450999 1108 5 0.09007034840569617 1108 6 0.20038205620238614 1108 7 0.22113720858261843 1108 8 0.14201921142753804 1108 9 0.2857355391089058 1108 10-11 0.24426114914473374 1108 12-13 0.1988478555574673 1108 14-15 0.336000268257834 1108 16-17 0.4253923212468038 1108 18-19 0.29423562718577045 1108 20-21 0.25974997276489376 1108 22-23 0.1002799428415102 1108 24-25 0.2297505760718579 1108 26-27 0.27261890017135215 1108 28-29 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64-65 -1.388964784485843 2316 66-67 -1.4927555360815177 2316 68-69 -1.4908024338860528 2316 70-71 -1.5854924900544098 2316 72-73 -1.7732339966937438 2316 74-75 -1.8339272993124531 2316 76-77 -1.9725965218024086 2316 78-79 -2.985175307716453 2316 80-81 -2.8416029107141085 2316 82-83 -2.457734142759591 2316 84-85 -2.510345068946041 2316 86-87 -2.40464934093691 2316 88-89 -2.8032700855256643 2316 90-91 -2.942992913235827 2316 92-93 -3.026936638832119 2316 94-95 -3.1224766248758336 2316 96-97 -3.6324559295886036 2316 98-99 -3.671036146108282 2316 100-101 -3.315637687131826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6303.0 3 1926.0 4 2172.0 5 2253.0 6 2601.0 7 3356.0 8 4076.0 9 5225.0 10 6339.0 11 7965.0 12 9996.0 13 12186.0 14 14113.0 15 17041.0 16 21531.0 17 27634.0 18 26596.0 19 20708.0 20 20883.0 21 22281.0 22 24464.0 23 27047.0 24 30381.0 25 34193.0 26 38982.0 27 44879.0 28 51571.0 29 59755.0 30 70034.0 31 83223.0 32 102086.0 33 126772.0 34 164522.0 35 221004.0 36 270902.0 37 292953.0 38 144228.0 39 6861.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.353376753343284 18.569209492057187 14.838383183155823 22.23903057144371 2 15.540265701614475 19.94088397926255 40.663144686337496 23.85570563278548 3 19.172247362267644 24.866504632951226 29.828947426814857 26.132300577966273 4 12.68364150653101 16.947635541633957 36.48765400441055 33.88106894742448 5 13.80268795962388 37.358515135865424 33.24370837211574 15.59508853239496 6 31.64340553778464 35.70369128096106 17.321570822923576 15.331332358330723 7 28.713132961714738 33.61618094094617 19.788912091081894 17.881774006257196 8 26.958639293041397 34.480354564015734 19.11910228919411 19.441903853748755 9 27.321779271028046 16.733683778177387 19.906226994969156 36.03830995582541 10-11 25.52170998440103 26.81947921086519 26.083835034049248 21.57497577068454 12-13 26.492110662254692 24.38959389423786 27.173062500832053 21.945232942675396 14-15 23.274090939794036 25.249763216138675 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40-41 22.000369726771915 26.04256787567321 30.0290358758211 21.928026521733774 42-43 22.097252173432935 25.955587061668535 29.75272001086501 22.194440754033522 44-45 21.938763564342967 26.315041007575996 29.508325608804213 22.237869819276828 46-47 22.08996560944636 26.61863853635066 29.108108401233647 22.183287452969342 48-49 22.389841053242876 26.534771390175294 28.843842526268464 22.231545030313367 50-51 22.215548525307415 26.775477228206917 28.631843935063646 22.37713031142203 52-53 22.44368867838731 26.83278129658927 28.503186400943065 22.220343624080353 54-55 22.097322061017792 26.706953808054173 28.54841579800137 22.647308332926666 56-57 22.203417108512898 27.11268551565076 28.45460167047575 22.22929570536059 58-59 22.418006706648928 26.60083904584783 28.46172956224402 22.519424685259224 60-61 22.256020691169653 26.627311249640474 28.895448125059918 22.221219934129955 62-63 22.195102895294177 26.668263757595117 28.819875500846976 22.316757846263734 64-65 22.07917297621301 26.564052739920502 28.710555476686228 22.64621880718026 66-67 22.04674615418739 26.89685680909687 28.50482790573878 22.55156913097697 68-69 21.952326145944497 26.671012453266364 28.68615945011423 22.69050195067491 70-71 21.96303616404634 26.811338625993482 28.55825660159164 22.667368608368534 72-73 22.186690681880243 26.667435626707363 28.337459099705526 22.808414591706867 74-75 22.012044783961844 27.02517594473871 28.145514358017355 22.81726491328209 76-77 22.09133394403029 26.96537584848801 28.16150888973174 22.781781317749964 78-79 22.257452262610663 26.93350690666252 27.9441394232203 22.864901407506522 80-81 22.03813002994336 27.217993521350387 28.08067971844517 22.66319673026108 82-83 22.221436283912794 27.08969152633355 27.90466573783546 22.784206451918198 84-85 22.191459079118427 26.822903124441623 27.804388143018222 23.18124965342173 86-87 22.32374920679904 26.54667102028746 28.070430084341137 23.05914968857236 88-89 22.38369162335167 26.610752176102547 27.90072441851666 23.104831782029127 90-91 22.270494676390506 26.748272174804296 27.888718148191423 23.09251500061378 92-93 22.37418568701989 26.410555704528605 27.829441850560755 23.38581675789075 94-95 22.274915863135888 26.317133551387883 27.83910305283768 23.568847532638546 96-97 22.4365183946175 26.306740790366877 27.618349707493365 23.638391107522256 98-99 22.394188974184857 26.33351531815095 27.38231477293594 23.88998093472825 100-101 23.02524741479306 25.356968335842446 27.55423381623436 24.06355043313014 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2937.0 1 1922.0 2 1229.0 3 1553.5 4 1222.5 5 1092.5 6 2119.0 7 4800.5 8 7863.0 9 8739.5 10 6578.5 11 4233.5 12 3765.5 13 3807.0 14 3888.0 15 4022.0 16 4089.0 17 4128.0 18 4148.5 19 4214.0 20 4486.0 21 4993.5 22 5760.5 23 6704.0 24 7789.0 25 9204.0 26 11148.0 27 13325.0 28 15163.0 29 17384.5 30 20054.0 31 22693.0 32 25779.0 33 28966.5 34 32248.0 35 35253.0 36 38695.5 37 42668.0 38 45993.0 39 48278.0 40 51194.0 41 57094.5 42 62507.0 43 66709.0 44 71535.0 45 76521.5 46 78047.0 47 78186.5 48 81559.5 49 81722.0 50 78444.5 51 74563.5 52 70679.5 53 69621.5 54 69056.5 55 64829.0 56 58524.5 57 53256.0 58 47959.5 59 42320.0 60 36573.5 61 30682.5 62 25378.0 63 20396.0 64 16392.0 65 13320.0 66 10229.5 67 8104.0 68 6145.0 69 4055.0 70 2971.0 71 2272.0 72 1790.0 73 1423.0 74 1080.0 75 917.0 76 641.0 77 349.0 78 198.5 79 120.0 80 70.5 81 35.0 82 25.0 83 16.5 84 11.0 85 8.5 86 8.0 87 6.0 88 6.5 89 6.0 90 3.5 91 1.5 92 1.5 93 2.0 94 1.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4703204763627367 2 0.04075815089091305 3 0.05268496167156717 4 0.058056954957068405 5 0.08402980322733586 6 0.0836355284907853 7 0.048150802201235854 8 0.042433818521252886 9 0.046967977991584205 10-11 0.050442524107435924 12-13 0.04558801641365728 14-15 0.04046244483850014 16-17 0.0398217483916055 18-19 0.04755939009641003 20-21 0.05633200298465976 22-23 0.047362252728134754 24-25 0.0659670918591138 26-27 0.05682484640534794 28-29 0.04393699095435186 30-31 0.04600693332124224 32-33 0.04492267779572823 34-35 0.04950612160812837 36-37 0.043887706612283035 38-39 0.02730352550612555 40-41 0.025480004849579258 42-43 0.025208940968200755 44-45 0.016337759395813393 46-47 0.0284863497157772 48-49 0.027796368926813735 50-51 0.017150951039948903 52-53 0.0168798871585704 54-55 0.01749594143443063 56-57 0.016682749790295125 58-59 0.015228861699264972 60-61 0.01727416189512095 62-63 0.017422014921327406 64-65 0.018235206565462913 66-67 0.010448280518589561 68-69 0.012666075911686402 70-71 0.018900545183391966 72-73 0.01616526419857253 74-75 0.01379961577926923 76-77 0.01047292268962397 78-79 0.015401356896505838 80-81 0.01865412347304787 82-83 0.016140622027538118 84-85 0.013774973608234823 86-87 0.003918105194471085 88-89 0.028954550965430974 90-91 0.030802713793011676 92-93 0.016658107619260717 94-95 0.03205946451576655 96-97 0.02666282905923091 98-99 0.023878263732342652 100-101 0.15238718567678736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2029042.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.482676242650726 #Duplication Level Percentage of deduplicated Percentage of total 1 75.53189013780724 38.13051955822387 2 13.248086243343163 13.375976973148154 3 4.574381526588667 6.92781064851414 4 2.0970859195343223 4.234660381154907 5 1.1884210034433988 2.99973363783996 6 0.7117961238396266 2.156002396234176 7 0.45968540777262323 1.6244304729839354 8 0.3445608957131729 1.3915484915332677 9 0.2684141967314552 1.219524029327269 >10 1.3732346919010459 12.674210038753925 >50 0.12384782581210485 4.323500652464591 >100 0.07427416927583973 6.964580921419153 >500 0.0024556012712686334 0.8170802432752384 >1k 0.001571584813611925 1.2492729487241383 >5k 9.822405085074531E-5 0.4278787121751445 >10k+ 1.9644810170149063E-4 1.4832698942281952 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16378 0.8071789544031124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13535 0.6670635699014609 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8629 0.42527458771183646 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3239 0.15963198396090372 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2509 0.12365441425066608 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2425 0.1195145295168853 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.928434206881868E-5 0.0 9 0.0 0.0 0.0 4.928434206881868E-5 0.0 10-11 0.0 0.0 0.0 4.928434206881868E-5 0.0 12-13 0.0 0.0 0.0 4.928434206881868E-5 0.0 14-15 0.0 0.0 0.0 4.928434206881868E-5 0.0 16-17 0.0 0.0 0.0 4.928434206881868E-5 0.0 18-19 0.0 0.0 0.0 1.232108551720467E-4 0.0 20-21 0.0 0.0 0.0 1.9713736827527473E-4 0.0 22-23 0.0 0.0 0.0 4.435590786193682E-4 0.0 24-25 0.0 0.0 0.0 7.392651310322802E-4 0.0 26-27 0.0 0.0 0.0 0.0015524567751677885 0.0 28-29 0.0 0.0 0.0 0.0044848751282625 0.0 30-31 0.0 0.0 0.0 0.01382425795030364 0.0 32-33 0.0 0.0 0.0 0.03348871043576229 0.0 34-35 0.0 0.0 0.0 0.06029939252119966 0.0 36-37 0.0 0.0 0.0 0.10536992334313434 2.464217103440934E-5 38-39 0.0 0.0 0.0 0.17269233460914069 4.928434206881868E-5 40-41 0.0 0.0 0.0 0.2416904135054868 4.928434206881868E-5 42-43 0.0 0.0 0.0 0.3109102719411427 4.928434206881868E-5 44-45 0.0 0.0 0.0 0.3911451808291795 4.928434206881868E-5 46-47 0.0 0.0 0.0 0.4885556829281996 4.928434206881868E-5 48-49 0.0 0.0 0.0 0.5912149674575489 4.928434206881868E-5 50-51 0.0 0.0 0.0 0.7003797851399823 4.928434206881868E-5 52-53 0.0 0.0 0.0 0.8024969419065746 4.928434206881868E-5 54-55 0.0 0.0 0.0 0.9114153378786639 4.928434206881868E-5 56-57 4.928434206881868E-5 0.0 0.0 1.0393574898893172 4.928434206881868E-5 58-59 4.928434206881868E-5 0.0 0.0 1.1806803407716548 4.928434206881868E-5 60-61 4.928434206881868E-5 0.0 0.0 1.3260198655326012 4.928434206881868E-5 62-63 4.928434206881868E-5 0.0 0.0 1.4712854637804442 4.928434206881868E-5 64-65 4.928434206881868E-5 0.0 0.0 1.617857097093111 4.928434206881868E-5 66-67 4.928434206881868E-5 0.0 0.0 1.7825160839450342 4.928434206881868E-5 68-69 4.928434206881868E-5 0.0 0.0 1.971176545384472 4.928434206881868E-5 70-71 4.928434206881868E-5 0.0 0.0 2.1725030827355964 4.928434206881868E-5 72-73 4.928434206881868E-5 0.0 0.0 2.3763924058742996 4.928434206881868E-5 74-75 4.928434206881868E-5 0.0 0.0 2.577176815462667 4.928434206881868E-5 76-77 4.928434206881868E-5 0.0 0.0 2.7947179013544323 4.928434206881868E-5 78-79 4.928434206881868E-5 0.0 0.0 3.0312827432847618 4.928434206881868E-5 80-81 4.928434206881868E-5 0.0 0.0 3.285861012241245 4.928434206881868E-5 82-83 4.928434206881868E-5 0.0 0.0 3.5435441947480637 4.928434206881868E-5 84-85 4.928434206881868E-5 0.0 0.0 3.801178092912813 4.928434206881868E-5 86-87 4.928434206881868E-5 0.0 0.0 4.068397795609949 4.928434206881868E-5 88-89 4.928434206881868E-5 0.0 0.0 4.35555301467392 4.928434206881868E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6660 0.0 54.005116 1 GTATCAA 11495 0.0 41.30395 1 TATCAAC 14255 0.0 32.97493 2 ATCAACG 14475 0.0 32.441723 3 TCAACGC 14580 0.0 32.175488 4 CAACGCA 14835 0.0 31.558344 5 AACGCAG 15355 0.0 30.546244 6 GTGGTAT 2870 0.0 27.294561 1 ACGCAGA 17075 0.0 27.21005 7 CGCAGAG 17130 0.0 27.178154 8 TGGTATC 3065 0.0 25.431276 2 GCAGAGT 17965 0.0 25.226772 9 GAGTACT 10235 0.0 24.901321 12-13 GTACATG 11360 0.0 24.555494 1 GTACTTT 10865 0.0 23.436728 14-15 TACATGG 11680 0.0 23.357363 2 GTATAGG 415 0.0 23.019508 1 ACATGGG 11725 0.0 22.6197 3 CAGAGTA 17685 0.0 22.239943 10-11 AGTACTT 10480 0.0 21.599424 12-13 >>END_MODULE