FastQCFastQC Report
Thu 26 May 2016
SRR938142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029042
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160340.7902251407314388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134640.6635643816145748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91180.4493746309834888No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34100.16805960645467172No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26510.13065279082443834No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24930.12286586477756499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66750.051.454951
GTATCAA114250.040.4572871
TATCAAC142450.032.31482
ATCAACG144400.031.9065953
TCAACGC146400.031.5324944
CAACGCA148050.031.1810675
AACGCAG152450.030.281126
ACGCAGA172600.026.635927
CGCAGAG173550.026.3259228
GTGGTAT31850.026.250851
GTACATG109400.025.4460541
GAGTACT102150.025.10663412-13
TACATGG111950.024.5269682
TGGTATC32350.024.5234932
GCAGAGT186400.024.3327169
GTACTTT107850.023.58155414-15
ACATGGG113450.023.3617633
AGTACTT104800.021.88864712-13
CAGAGTA181750.021.47981310-11
CATGGGA67050.020.6831744
Produced by FastQC (version 0.11.5)