##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938142_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2029042 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49843867204326 33.0 31.0 34.0 31.0 34.0 2 32.72583169791458 34.0 31.0 34.0 31.0 34.0 3 32.67911457722413 34.0 31.0 34.0 31.0 34.0 4 36.210226303841914 37.0 35.0 37.0 35.0 37.0 5 36.17975330229734 37.0 35.0 37.0 35.0 37.0 6 36.24285993094278 37.0 37.0 37.0 35.0 37.0 7 36.20360692385865 37.0 37.0 37.0 35.0 37.0 8 36.20164639273115 37.0 37.0 37.0 35.0 37.0 9 37.896106635545245 39.0 38.0 39.0 35.0 39.0 10-11 37.86600449867474 39.0 38.0 39.0 35.0 39.0 12-13 37.800577563204705 39.0 38.0 39.0 35.0 39.0 14-15 39.05039989315155 40.0 38.0 41.0 36.0 41.0 16-17 38.989076618423866 40.0 38.0 41.0 36.0 41.0 18-19 39.0056053546452 40.0 38.5 41.0 36.0 41.0 20-21 39.0334633289996 40.0 38.5 41.0 35.5 41.0 22-23 39.05340894865656 40.0 39.0 41.0 35.0 41.0 24-25 38.99998718607107 40.0 38.5 41.0 35.0 41.0 26-27 38.83457661300259 40.0 38.0 41.0 35.0 41.0 28-29 38.74667848176627 40.0 38.0 41.0 35.0 41.0 30-31 38.51876797030323 40.0 38.0 41.0 34.5 41.0 32-33 38.30458856938398 40.0 38.0 41.0 34.0 41.0 34-35 38.093918952885154 40.0 37.0 41.0 34.0 41.0 36-37 38.0098122660842 40.0 37.0 41.0 33.5 41.0 38-39 37.974858332158725 40.0 37.0 41.0 33.0 41.0 40-41 37.84111763088196 40.0 37.0 41.0 33.0 41.0 42-43 37.63751711398778 40.0 37.0 41.0 33.0 41.0 44-45 37.354835434653396 40.0 37.0 41.0 33.0 41.0 46-47 36.936359375508246 40.0 36.0 41.0 31.5 41.0 48-49 36.52811154229434 40.0 36.0 41.0 30.5 41.0 50-51 36.223810054202914 39.0 35.0 41.0 30.0 41.0 52-53 35.76771008190072 39.0 35.0 40.0 28.5 41.0 54-55 35.46510126453765 38.5 35.0 40.0 28.0 41.0 56-57 35.21187979351832 38.0 35.0 40.0 27.0 41.0 58-59 34.847747114155354 38.0 34.0 40.0 26.5 41.0 60-61 34.454631791751964 37.5 34.0 40.0 26.0 41.0 62-63 34.03683610294908 37.0 33.5 40.0 25.5 41.0 64-65 33.610439803611754 36.0 33.0 39.0 24.0 41.0 66-67 33.10961823362946 36.0 32.5 39.0 22.5 40.0 68-69 32.592423419525076 35.0 32.0 38.5 21.5 40.0 70-71 32.019434048186284 35.0 31.0 38.0 19.5 40.0 72-73 31.554923456488332 35.0 31.0 37.0 18.5 39.0 74-75 31.002582992367827 34.5 30.5 36.5 16.5 39.0 76-77 29.77690456875708 33.5 29.0 35.0 14.0 37.5 78-79 30.48187519036077 34.0 30.0 36.0 14.0 37.5 80-81 30.29918035210705 34.0 30.0 35.0 9.5 37.0 82-83 29.85911947608773 34.0 30.0 35.0 7.0 37.0 84-85 29.36793989478779 34.0 29.0 35.0 2.0 36.0 86-87 28.826494720168434 34.0 29.0 35.0 2.0 36.0 88-89 28.240959033869185 33.5 28.0 35.0 2.0 35.0 90-91 27.72999055712006 33.0 27.0 35.0 2.0 35.0 92-93 27.10482138861591 33.0 25.5 35.0 2.0 35.0 94-95 26.37706735493893 32.5 25.0 34.5 2.0 35.0 96-97 25.581627191551483 32.0 22.5 34.0 2.0 35.0 98-99 24.584594108944025 32.0 10.0 34.0 2.0 35.0 100-101 21.131340553818006 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08822167233105915 1101 2 0.07066469193257063 1101 3 0.194474229277958 1101 4 0.11914682043911284 1101 5 0.048724620676544816 1101 6 0.09490010626176115 1101 7 0.10582072817082633 1101 8 0.1232078747843417 1101 9 0.21459597656553342 1101 10-11 0.16504862261938058 1101 12-13 0.111220072633337 1101 14-15 0.2746311623935327 1101 16-17 0.32741043952980675 1101 18-19 0.2539767728087341 1101 20-21 0.20882095289238123 1101 22-23 0.17614161352932456 1101 24-25 0.17075484951414666 1101 26-27 0.229386913923193 1101 28-29 0.23470751805756151 1101 30-31 0.25855612928469185 1101 32-33 0.31785469812092515 1101 34-35 0.17377907156004113 1101 36-37 0.24052837512748937 1101 38-39 0.2959651558831311 1101 40-41 0.2575898671787158 1101 42-43 0.23985787772844702 1101 44-45 0.2534290403876156 1101 46-47 0.32226433704327917 1101 48-49 0.18217727795227034 1101 50-51 0.11035578533657997 1101 52-53 0.22335881223460774 1101 54-55 0.23298796664551702 1101 56-57 0.2948395631434835 1101 58-59 0.38217918003452667 1101 60-61 0.5086491986209296 1101 62-63 0.4697234632702205 1101 64-65 0.47945968683791307 1101 66-67 0.5470667255634751 1101 68-69 0.6946985274291464 1101 70-71 0.70889032753594 1101 72-73 0.5575482919842081 1101 74-75 0.7523390834972012 1101 76-77 0.8656178572314346 1101 78-79 0.9044392838521418 1101 80-81 0.9795320808797037 1101 82-83 1.006041548269284 1101 84-85 1.0026976683118178 1101 86-87 1.2267908917930335 1101 88-89 1.4911009868013032 1101 90-91 1.5824999144422947 1101 92-93 1.742240422409683 1101 94-95 1.9703146014332198 1101 96-97 2.193292274207078 1101 98-99 2.4971330101889357 1101 100-101 2.7817101720838657 1102 1 0.1287593226002528 1102 2 0.10971255681936753 1102 3 0.22293257183025617 1102 4 0.0950853114782717 1102 5 -0.014147518370087653 1102 6 0.0376847265631568 1102 7 0.05734861862489282 1102 8 0.09706656642826772 1102 9 0.13447944879799678 1102 10-11 0.12802770170583955 1102 12-13 0.1652702729922595 1102 14-15 0.31969225342777463 1102 16-17 0.28709588830921007 1102 18-19 0.3111528182398189 1102 20-21 0.35423359394260956 1102 22-23 0.33195509534952095 1102 24-25 0.2941187898243953 1102 26-27 0.3107953265396617 1102 28-29 0.09492152105680418 1102 30-31 0.14621131526202902 1102 32-33 0.2652733868147763 1102 34-35 0.13665489819425858 1102 36-37 0.27569198109646464 1102 38-39 0.36247632927350537 1102 40-41 0.22399963191062966 1102 42-43 0.37503131726430183 1102 44-45 0.3769613805306946 1102 46-47 0.37359186857214866 1102 48-49 0.22216916525959363 1102 50-51 0.3462513774402254 1102 52-53 0.3833214446804618 1102 54-55 0.4473431304973303 1102 56-57 0.4305126707964533 1102 58-59 0.38438620120128064 1102 60-61 0.4973756287903157 1102 62-63 0.47489714864524757 1102 64-65 0.6466733456623146 1102 66-67 0.7004144404883661 1102 68-69 0.7152555989929326 1102 70-71 0.8285715970494181 1102 72-73 0.6937812983514426 1102 74-75 0.9051189099347461 1102 76-77 0.821718970882884 1102 78-79 0.9883614020024467 1102 80-81 1.0649839332778654 1102 82-83 0.9609312894006372 1102 84-85 0.9922519618929542 1102 86-87 1.1136391106882293 1102 88-89 1.2619598259487326 1102 90-91 1.239785972902844 1102 92-93 1.4168132768483055 1102 94-95 1.4467078949406087 1102 96-97 1.7586400137158904 1102 98-99 2.007434900692168 1102 100-101 2.477002351939799 1103 1 0.2931771298569359 1103 2 0.25131060071920075 1103 3 0.3092061448045982 1103 4 0.10542791005616436 1103 5 0.09411531853724853 1103 6 0.10124533475292452 1103 7 0.1969554312496058 1103 8 0.18077837891246418 1103 9 0.15876769252358258 1103 10-11 0.20894026740101168 1103 12-13 0.19587509016997018 1103 14-15 0.32765015230638994 1103 16-17 0.31565045394817304 1103 18-19 0.4046015689775686 1103 20-21 0.3993215622403028 1103 22-23 0.4117146580374751 1103 24-25 0.36215088560584974 1103 26-27 0.3178503559092718 1103 28-29 0.42580317137579726 1103 30-31 0.426249818957821 1103 32-33 0.4753904436709604 1103 34-35 0.43334554769820954 1103 36-37 0.4630447817485006 1103 38-39 0.35552247749868116 1103 40-41 0.5500704281220834 1103 42-43 0.5601202692330531 1103 44-45 0.5486323566134317 1103 46-47 0.5374735751009183 1103 48-49 0.5316039759731765 1103 50-51 0.5024211028554788 1103 52-53 0.6604279059940836 1103 54-55 0.686386440128878 1103 56-57 0.6609038908296228 1103 58-59 0.5937409129856519 1103 60-61 0.7229236740953908 1103 62-63 0.7207383279263198 1103 64-65 0.7244711872069516 1103 66-67 0.6972266436304935 1103 68-69 0.8447003869415539 1103 70-71 1.0642359965885007 1103 72-73 0.9283229076372912 1103 74-75 0.6619202926952106 1103 76-77 0.423975793344308 1103 78-79 0.7882834109398011 1103 80-81 0.9509792678665505 1103 82-83 1.016082915268374 1103 84-85 1.1063282947672448 1103 86-87 1.168641078030351 1103 88-89 1.2793096755875872 1103 90-91 1.315029080179201 1103 92-93 1.7687377590157567 1103 94-95 1.7351799727349366 1103 96-97 1.8521604319166158 1103 98-99 2.421208403404293 1103 100-101 2.5058007274773075 1104 1 0.04479868863962366 1104 2 0.08434063144743931 1104 3 0.149897498795184 1104 4 0.05462577101873478 1104 5 0.03529493107235737 1104 6 0.0281064669849016 1104 7 0.08769799897427077 1104 8 0.033948603310307135 1104 9 0.044362808681356114 1104 10-11 0.12075524443338992 1104 12-13 0.0924505251725094 1104 14-15 0.1636631473986654 1104 16-17 0.13531118652451113 1104 18-19 0.10286988495688121 1104 20-21 0.1995119642209815 1104 22-23 0.16035372374815182 1104 24-25 0.08690235760796128 1104 26-27 0.1171531578974907 1104 28-29 0.14168084281612892 1104 30-31 0.14181031086102536 1104 32-33 0.22755367409506277 1104 34-35 0.1959719325112914 1104 36-37 0.158225663630148 1104 38-39 0.1633167051138784 1104 40-41 0.17999228790328914 1104 42-43 0.24859356582655323 1104 44-45 0.12809470666402234 1104 46-47 0.172399142379426 1104 48-49 0.0893479324383577 1104 50-51 0.10154135773020556 1104 52-53 0.15839569475470938 1104 54-55 0.23941794757214296 1104 56-57 0.353484968768754 1104 58-59 0.22788931970438853 1104 60-61 0.3196378260525137 1104 62-63 0.1843545831026816 1104 64-65 0.3393833383723077 1104 66-67 0.39205185712578583 1104 68-69 0.5176903264276547 1104 70-71 0.39611418959201217 1104 72-73 0.4405824701526093 1104 74-75 0.6430660978819347 1104 76-77 0.6186153177792306 1104 78-79 0.6988549624960108 1104 80-81 0.8259621442560778 1104 82-83 0.8959084743778227 1104 84-85 0.8840588036998014 1104 86-87 0.8936098156589374 1104 88-89 1.1154772544661604 1104 90-91 1.1614906196074912 1104 92-93 1.355623115658144 1104 94-95 1.6354995087322202 1104 96-97 1.9484738285497087 1104 98-99 2.1716740399313075 1104 100-101 2.540703515640967 1105 1 0.08282308324867671 1105 2 0.11699138508227236 1105 3 0.14034588692808825 1105 4 0.07374606220849245 1105 5 0.02308577414540025 1105 6 0.043696876497158144 1105 7 0.061433318295698314 1105 8 0.0493038612126 1105 9 0.04240526634755071 1105 10-11 0.06216705487558727 1105 12-13 0.06979217414946959 1105 14-15 0.03325676417028944 1105 16-17 0.06482816567969252 1105 18-19 0.08821782992855987 1105 20-21 0.08033169988875954 1105 22-23 0.09990778906198017 1105 24-25 0.07633215051098574 1105 26-27 0.20007467960338943 1105 28-29 0.11015227438451802 1105 30-31 0.06375609561433038 1105 32-33 0.06539862337271529 1105 34-35 0.1008426761664083 1105 36-37 0.1268399166148768 1105 38-39 0.10896029634357518 1105 40-41 0.17446373606503585 1105 42-43 0.1647774249915983 1105 44-45 0.2063463828896417 1105 46-47 0.16941506370794457 1105 48-49 0.25711082755291415 1105 50-51 0.31180814339496976 1105 52-53 0.2963300111260807 1105 54-55 0.39691891650581823 1105 56-57 0.3749037958315782 1105 58-59 0.4723607086041426 1105 60-61 0.5049543621650017 1105 62-63 0.6235553283815776 1105 64-65 0.6750205411585668 1105 66-67 0.8053572425006337 1105 68-69 0.8182958655281354 1105 70-71 0.8674493979749798 1105 72-73 0.6899509150348031 1105 74-75 0.7108140589728755 1105 76-77 0.7449917665869386 1105 78-79 0.8177955713063731 1105 80-81 1.0620871918138306 1105 82-83 0.917436699089837 1105 84-85 1.0705313043546418 1105 86-87 1.1138608257796996 1105 88-89 1.1078246340783728 1105 90-91 1.0535077913641722 1105 92-93 1.3209448905312975 1105 94-95 1.4261485579919793 1105 96-97 1.6669245776806285 1105 98-99 2.055812921609988 1105 100-101 2.274595689619968 1106 1 0.10364359973853254 1106 2 0.11435798410172993 1106 3 0.19573756615731241 1106 4 0.06837450410823465 1106 5 0.05898719955376919 1106 6 0.05628374751312748 1106 7 0.03610594951503998 1106 8 -0.012951917269319324 1106 9 0.09256007394115073 1106 10-11 0.13192073063522258 1106 12-13 0.12231019138700105 1106 14-15 0.18692630391436182 1106 16-17 0.1735396675062617 1106 18-19 0.149844603914147 1106 20-21 0.11538946424778374 1106 22-23 0.14751442695344252 1106 24-25 0.154579257616426 1106 26-27 0.12086641153803868 1106 28-29 0.07511906224561926 1106 30-31 0.08920961604025734 1106 32-33 0.18437271937751376 1106 34-35 -0.030878114115772348 1106 36-37 0.06681874090960349 1106 38-39 0.0944585859891589 1106 40-41 0.0808916008239251 1106 42-43 0.03623006761719694 1106 44-45 0.08224987536112138 1106 46-47 -0.23753284045609746 1106 48-49 0.1426253450468451 1106 50-51 0.18884045341514621 1106 52-53 -0.015351537693440775 1106 54-55 0.07915040465797318 1106 56-57 0.20223913870567145 1106 58-59 0.18833396211704212 1106 60-61 0.2611194830940349 1106 62-63 0.12163253880041935 1106 64-65 0.1858859507983297 1106 66-67 0.2542593958238726 1106 68-69 -0.02050969000793401 1106 70-71 0.15019206334996937 1106 72-73 0.32684088297428104 1106 74-75 0.46652660064442486 1106 76-77 0.4725632550524246 1106 78-79 0.6031944361520125 1106 80-81 0.7113569737129524 1106 82-83 0.5772563361425505 1106 84-85 0.7844160992582587 1106 86-87 0.9773821659848458 1106 88-89 1.1314964817311477 1106 90-91 1.2174546566185498 1106 92-93 1.1690417076516297 1106 94-95 1.4527104357831853 1106 96-97 1.5420492971130564 1106 98-99 1.6454469976596684 1106 100-101 2.0082270167563223 1107 1 0.012163752207214884 1107 2 0.05632389203829291 1107 3 0.14848549991483395 1107 4 0.09272831545039395 1107 5 0.0027445440915911945 1107 6 0.053635793780046015 1107 7 -0.01006121017734074 1107 8 -0.0015156296222826882 1107 9 -0.007672656012339019 1107 10-11 -0.015615879279508249 1107 12-13 0.009516497934690449 1107 14-15 0.06859592828081418 1107 16-17 0.1475140683476397 1107 18-19 0.016574037901534666 1107 20-21 0.04824308890147222 1107 22-23 0.035550093881226985 1107 24-25 0.10889535175285658 1107 26-27 0.12175303971256568 1107 28-29 0.10717107412914118 1107 30-31 0.11951621008591218 1107 32-33 0.13259911078607445 1107 34-35 0.08674982202335713 1107 36-37 0.12399983826508532 1107 38-39 0.23460594602337181 1107 40-41 0.24138164043187516 1107 42-43 0.26658670280703234 1107 44-45 0.19512583445464315 1107 46-47 0.4400203884816847 1107 48-49 0.4348423336289642 1107 50-51 0.38827043433269637 1107 52-53 0.3575087318424366 1107 54-55 0.3312080115141143 1107 56-57 0.22733845401464947 1107 58-59 0.19701287363807296 1107 60-61 0.211090383327857 1107 62-63 0.35459156346625065 1107 64-65 0.5235943618238323 1107 66-67 0.5034299786304857 1107 68-69 0.6690859107346299 1107 70-71 0.7458888203793066 1107 72-73 0.4846419496551242 1107 74-75 0.49786992357183735 1107 76-77 0.5256387969546097 1107 78-79 0.7148105037680033 1107 80-81 0.7825928831652966 1107 82-83 0.8362142577215828 1107 84-85 0.9195861607461424 1107 86-87 1.1210492228451763 1107 88-89 1.202756593644235 1107 90-91 1.1337542526306237 1107 92-93 1.2966868491269423 1107 94-95 1.4201556262997315 1107 96-97 1.5348131022307498 1107 98-99 1.6681005009147327 1107 100-101 1.9415490101573454 1108 1 0.0915927913822756 1108 2 0.09489682592646176 1108 3 0.18993727131084626 1108 4 0.10252321119913432 1108 5 0.021950146023392847 1108 6 0.004719460318149515 1108 7 0.02900509719455613 1108 8 0.03336917057653466 1108 9 0.06652955303459862 1108 10-11 0.08868103551029805 1108 12-13 0.0918479557596541 1108 14-15 0.13632947472737555 1108 16-17 0.0792852102413022 1108 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-0.7780874950693786 2316 52-53 -0.6946201259440343 2316 54-55 -0.834022455136548 2316 56-57 -0.9017351626708887 2316 58-59 -1.0329214911064213 2316 60-61 -0.9526824432189755 2316 62-63 -0.9448138314315884 2316 64-65 -0.9453752561423912 2316 66-67 -0.8413683701968822 2316 68-69 -0.9540042062424874 2316 70-71 -1.1302486477464342 2316 72-73 -1.083986056245184 2316 74-75 -1.0101629626642001 2316 76-77 -0.9460957347366978 2316 78-79 -1.1801121975687145 2316 80-81 -1.451781009096834 2316 82-83 -1.3643300346411742 2316 84-85 -1.4201620468625151 2316 86-87 -1.595022552851482 2316 88-89 -1.6364498944122055 2316 90-91 -1.9894622179307078 2316 92-93 -2.48004636757231 2316 94-95 -2.584461139277206 2316 96-97 -2.8989473176519276 2316 98-99 -3.164355504025064 2316 100-101 -3.6810674238129018 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 16.0 5 42.0 6 90.0 7 197.0 8 459.0 9 843.0 10 1439.0 11 2452.0 12 3711.0 13 5736.0 14 7803.0 15 10479.0 16 14492.0 17 21646.0 18 21923.0 19 14505.0 20 12419.0 21 12874.0 22 13118.0 23 14261.0 24 16268.0 25 19034.0 26 22703.0 27 26666.0 28 32603.0 29 40055.0 30 48590.0 31 60056.0 32 75882.0 33 99149.0 34 139155.0 35 212063.0 36 312863.0 37 444821.0 38 301184.0 39 19432.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.632463004708626 18.966536917422115 15.173613951805828 22.227386126063433 2 15.700469876495383 20.317315735021793 40.17106402652562 23.811150361957207 3 19.43825746056609 24.857940333214877 29.951436763055465 25.752365443163566 4 12.997266690388864 17.194222692285326 36.23286260215412 33.575648015171694 5 13.78004815096832 37.55662174383389 33.27424120333065 15.389088901867144 6 31.709444717425345 35.885707470383764 17.171133723895892 15.233714088295 7 28.659732031175306 33.595213898973014 19.739512538429466 18.005541531422217 8 26.983401017229824 34.67398375586484 19.049402673185348 19.293212553719986 9 27.084998733392407 16.73627258578186 19.8983066885752 36.28042199225053 10-11 25.529535613358423 26.901389917015024 26.042758109492066 21.526316360134487 12-13 26.3939829732455 24.358465719290187 27.236424874398857 22.011126433065456 14-15 23.102823894231857 25.283409609066744 26.208402783185363 25.40536371351603 16-17 21.999599318200243 28.981492000040415 26.633240374827228 22.385668306932118 18-19 21.790036874544736 26.574363665217376 29.936171848586675 21.699427611651213 20-21 22.717814613990246 25.649050142875307 30.14035687777779 21.492778365356656 22-23 22.51569952716602 25.76972285443081 30.098046270111706 21.61653134829146 24-25 22.202037202918177 25.99763677561566 30.17265830707871 21.627667714387453 26-27 21.952171743001685 26.36696264072118 29.99735040575763 21.683515210519506 28-29 21.57834713464634 26.39773409415515 30.419352263195194 21.604566508003316 30-31 21.805056927209613 25.914244604032895 30.42421324188772 21.856485226869772 32-33 21.615129844206667 26.168499287815983 30.235760016165834 21.980610851811512 34-35 22.221460935127823 26.17327211927868 29.85836445299715 21.746902492596345 36-37 22.25489473669943 25.942381752373283 29.81694164070623 21.98578187022106 38-39 21.854022906631656 26.050335041960654 30.050430673167288 22.0452113782404 40-41 21.98044152001668 26.036524017010887 30.09738817687026 21.88564628610217 42-43 22.157940774366203 25.911738529590018 29.82088471460672 22.10943598143706 44-45 21.932051437185766 26.24645518493664 29.597502222325183 22.22399115555241 46-47 22.078374288348243 26.623359501467352 29.112498826389132 22.185767383795273 48-49 22.31263459282275 26.547033530446594 28.911333039105013 22.228998837625642 50-51 22.17319799195877 26.71130020965559 28.765644082281195 22.349857716104445 52-53 22.408801789218753 26.769258595928523 28.59746126497135 22.224478349881373 54-55 22.149886498160214 26.7176086054404 28.527526783575695 22.604978112823687 56-57 22.184804454515973 27.104096415944074 28.499114360373024 22.211984769166925 58-59 22.447243576032434 26.67221772639502 28.428711677727712 22.451827019844835 60-61 22.222975177448273 26.699077692822527 28.860171450369187 22.217775679360013 62-63 22.17176874603877 26.749939133837547 28.71409266047721 22.364199459646475 64-65 22.030766243379883 26.65075439542405 28.688292307404183 22.63018705379189 66-67 22.03261106077247 26.949873474367504 28.462562631358594 22.554952833501435 68-69 21.864318359970362 26.831084605920104 28.65828189882242 22.646315135287118 70-71 21.949521005479433 26.95831333210451 28.41180714839811 22.680358514017946 72-73 22.179062828664957 26.93349866587286 28.145622416884418 22.741816088577764 74-75 21.972881783620053 27.22030889454235 27.969085903593914 22.837723418243684 76-77 22.004522331228234 27.133469883817092 28.175513370349158 22.686494414605512 78-79 22.099025057145194 27.136870503419843 28.020686609739965 22.743417829694998 80-81 21.8809980806142 27.559955239795087 28.076728118718535 22.482318560872177 82-83 22.13727704148947 27.351343332572704 27.975831694201293 22.535547931736538 84-85 22.043427302935402 27.154878819332524 27.85299716011304 22.94869671761903 86-87 22.17285535030108 26.89167117621953 28.098802085335528 22.836671388143866 88-89 22.116531898566862 27.148490345931897 27.879796416139445 22.855181339361796 90-91 21.99338035412715 27.234537094241112 28.013630625140312 22.75845192649142 92-93 22.070403609213173 26.991549408226938 27.86709113889177 23.070955843668116 94-95 21.934806608051097 26.948901943776367 27.87002720498364 23.246264243188897 96-97 22.0924561978062 26.92398584254152 27.78360566702472 23.19995229262756 98-99 21.974291252451287 27.068382685973692 27.588654125084954 23.368671936490067 100-101 22.112067998916693 26.76681090216309 27.74969707588591 23.371424023034308 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2898.0 1 1881.5 2 1257.0 3 1630.0 4 1216.5 5 1053.0 6 2242.0 7 5212.0 8 8179.5 9 8839.0 10 6663.5 11 4256.0 12 3745.0 13 3869.0 14 3991.5 15 3974.5 16 3949.0 17 4056.5 18 4175.0 19 4264.0 20 4484.0 21 4940.5 22 5683.0 23 6634.0 24 7742.0 25 9259.0 26 11133.0 27 13283.0 28 15163.5 29 17095.5 30 19833.0 31 22648.5 32 25683.0 33 29112.5 34 32450.5 35 35253.0 36 38912.5 37 43049.0 38 46467.5 39 48695.5 40 51442.0 41 57549.5 42 63286.0 43 67560.0 44 72807.5 45 78325.0 46 79442.0 47 79544.5 48 83200.0 49 83207.0 50 79561.5 51 75013.0 52 70822.5 53 69839.0 54 69558.5 55 65235.0 56 57959.0 57 51926.0 58 47319.5 59 42021.5 60 35610.5 61 29286.5 62 24108.5 63 19144.0 64 15112.0 65 12160.5 66 8982.0 67 7291.5 68 5448.5 69 3277.5 70 2343.0 71 1790.0 72 1450.5 73 1223.0 74 963.5 75 775.0 76 517.5 77 250.5 78 119.5 79 68.5 80 29.5 81 14.5 82 7.0 83 5.5 84 4.5 85 3.0 86 3.0 87 2.5 88 1.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010398996176520743 3 0.006949092231703434 4 0.0 5 3.449903944817308E-4 6 1.4785302620645604E-4 7 0.0 8 9.856868413763738E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.4642171034409345E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017003098013742446 26-27 0.021167624918557625 28-29 3.942747365505495E-4 30-31 1.724951972408654E-4 32-33 0.003548472628954945 34-35 0.015475283409609067 36-37 0.007565146507563668 38-39 0.02047764412959416 40-41 0.022621513009587778 42-43 0.018555554788910234 44-45 0.00978294190066051 46-47 0.0023410062482688873 48-49 2.710638813785028E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.2034822344732144E-4 68-69 2.710638813785028E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.6963256551614016E-4 82-83 0.0010349711834451924 84-85 0.0043370221020560444 86-87 7.88549473101099E-4 88-89 4.928434206881869E-5 90-91 0.004016673878608722 92-93 6.160542758602335E-4 94-95 9.856868413763738E-5 96-97 2.710638813785028E-4 98-99 2.464217103440934E-4 100-101 0.00337597743171408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2029042.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.407631889994526 #Duplication Level Percentage of deduplicated Percentage of total 1 73.68453356178891 35.668937766450256 2 14.248896109279166 13.795106353935225 3 4.88123184260056 7.088666226189829 4 2.2737592026999747 4.402691939631513 5 1.268641612028612 3.0705968077705155 6 0.77907086600439 2.262778541865586 7 0.5166490798540262 1.750683093171466 8 0.3564074864757648 1.3802273926525621 9 0.26844603969566627 1.1695353364723204 >10 1.4981268734151196 13.227319379900724 >50 0.13566217645486006 4.549137663514448 >100 0.08306583394943186 7.337550699707138 >500 0.0034688284374840973 1.0458807697714507 >1k 0.001734414218742048 1.3437372613657093 >5k 1.0202436580835576E-4 0.4503159493212488 >10k+ 2.0404873161671152E-4 1.4568348182801267 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16034 0.7902251407314388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13464 0.6635643816145748 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9118 0.4493746309834888 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3410 0.16805960645467172 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2651 0.13065279082443834 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2493 0.12286586477756499 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.928434206881868E-5 0.0 9 0.0 0.0 0.0 4.928434206881868E-5 0.0 10-11 0.0 0.0 0.0 4.928434206881868E-5 0.0 12-13 0.0 0.0 0.0 4.928434206881868E-5 0.0 14-15 0.0 0.0 0.0 4.928434206881868E-5 0.0 16-17 0.0 0.0 0.0 4.928434206881868E-5 0.0 18-19 0.0 0.0 0.0 1.232108551720467E-4 0.0 20-21 0.0 0.0 0.0 1.9713736827527473E-4 0.0 22-23 0.0 0.0 0.0 4.189169075849588E-4 0.0 24-25 0.0 0.0 0.0 6.899807889634616E-4 0.0 26-27 0.0 0.0 0.0 0.0015031724330989698 0.0 28-29 0.0 0.0 0.0 0.004435590786193681 0.0 30-31 0.0 0.0 0.0 0.013873542292372459 0.0 32-33 0.0 0.0 0.0 0.0337597743171408 0.0 34-35 0.0 0.0 0.0 0.06116186850740399 0.0 36-37 0.0 0.0 0.0 0.10667595840795804 0.0 38-39 0.0 0.0 0.0 0.17429407572637728 0.0 40-41 0.0 0.0 0.0 0.2437849980434116 0.0 42-43 0.0 0.0 0.0 0.3129309299659643 0.0 44-45 0.0 0.0 0.0 0.3929933436567602 0.0 46-47 0.0 0.0 0.0 0.4909459735185373 0.0 48-49 0.0 0.0 0.0 0.593753111074093 0.0 50-51 0.0 0.0 0.0 0.7032382769799739 0.0 52-53 0.0 0.0 0.0 0.8057497084831167 0.0 54-55 0.0 0.0 0.0 0.9152102322179629 0.0 56-57 0.0 0.0 0.0 1.0440148602148205 0.0 58-59 0.0 0.0 0.0 1.1871612317537044 2.464217103440934E-5 60-61 0.0 0.0 0.0 1.333708222895337 4.928434206881868E-5 62-63 0.0 0.0 0.0 1.4803044983790379 4.928434206881868E-5 64-65 0.0 0.0 0.0 1.6281328824144596 4.928434206881868E-5 66-67 0.0 0.0 0.0 1.794122546502241 4.928434206881868E-5 68-69 0.0 0.0 0.0 1.9834729887306424 4.928434206881868E-5 70-71 0.0 0.0 0.0 2.1859330659493494 4.928434206881868E-5 72-73 0.0 0.0 0.0 2.3923605327045965 4.928434206881868E-5 74-75 0.0 0.0 0.0 2.595929507619852 4.928434206881868E-5 76-77 0.0 0.0 0.0 2.817733689100571 4.928434206881868E-5 78-79 0.0 0.0 0.0 3.059350176092954 4.928434206881868E-5 80-81 0.0 0.0 0.0 3.319078658795629 4.928434206881868E-5 82-83 0.0 0.0 0.0 3.582626678008636 4.928434206881868E-5 84-85 0.0 0.0 0.0 3.8464704032740573 4.928434206881868E-5 86-87 0.0 0.0 0.0 4.121082757281515 4.928434206881868E-5 88-89 0.0 0.0 0.0 4.420115502784073 4.928434206881868E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6675 0.0 51.45495 1 GTATCAA 11425 0.0 40.457287 1 TATCAAC 14245 0.0 32.3148 2 ATCAACG 14440 0.0 31.906595 3 TCAACGC 14640 0.0 31.532494 4 CAACGCA 14805 0.0 31.181067 5 AACGCAG 15245 0.0 30.28112 6 ACGCAGA 17260 0.0 26.63592 7 CGCAGAG 17355 0.0 26.325922 8 GTGGTAT 3185 0.0 26.25085 1 GTACATG 10940 0.0 25.446054 1 GAGTACT 10215 0.0 25.106634 12-13 TACATGG 11195 0.0 24.526968 2 TGGTATC 3235 0.0 24.523493 2 GCAGAGT 18640 0.0 24.332716 9 GTACTTT 10785 0.0 23.581554 14-15 ACATGGG 11345 0.0 23.361763 3 AGTACTT 10480 0.0 21.888647 12-13 CAGAGTA 18175 0.0 21.479813 10-11 CATGGGA 6705 0.0 20.683174 4 >>END_MODULE