FastQCFastQC Report
Thu 26 May 2016
SRR938141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051223
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164770.8032768743330199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136720.6665291877089912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87010.42418596125335956No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.16282968745962773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25420.12392606752166878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24830.12104973471923823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68700.050.3930241
GTATCAA119100.041.978221
TGCGTCG550.002575445534.5581635
TATCAAC148200.033.7624172
ATCAACG148850.033.391523
TCAACGC150200.033.059764
CAACGCA153350.032.3496865
AACGCAG157050.031.6783166
ACGCAGA176800.028.0485157
CGCAGAG177850.027.7759888
GTGGTAT31800.026.128311
GCAGAGT187000.025.7552079
GTACATG112600.025.317751
GAGTACT103300.025.1270712-13
TGGTATC32600.024.3417762
TACATGG116200.024.0858782
GTACTTT108500.023.92982914-15
ACATGGG119200.022.5628283
CAGAGTA185250.021.99479110-11
ACTTTTT122600.021.62255516-17
Produced by FastQC (version 0.11.5)