FastQCFastQC Report
Thu 26 May 2016
SRR938141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051223
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160390.7819237596302304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139400.6795945638285062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90050.4390063878963916No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35360.1723849625321089No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25290.12329229927706543No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23420.11417578683546353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69300.054.3662531
GTATCAA115700.039.2156561
TTCGCGA300.003949742831.67056822-23
TATCAAC146000.031.077822
ATCAACG147550.030.6824633
TCAACGC149150.030.4444144
CAACGCA151100.029.988655
GTGGTAT29050.029.4385091
AACGCAG156700.028.9472546
TGGTATC32750.025.6780472
ACGCAGA176550.025.5581197
CGCAGAG175950.025.5372928
GAGTACT104050.023.80593312-13
GCAGAGT188500.023.4585279
GTACATG115950.023.3557361
ACCGTCG1050.002197411422.6179738
TACATGG119750.022.4167652
GTACTTT109650.022.35188114-15
CAGAGTA183450.021.72260710-11
AGTACTT106650.021.6890812-13
Produced by FastQC (version 0.11.5)