##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938141_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2051223 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.509869965381625 34.0 31.0 34.0 31.0 34.0 2 32.722211578165805 34.0 31.0 34.0 31.0 34.0 3 32.771062921973865 34.0 31.0 34.0 31.0 34.0 4 36.23564283356807 37.0 35.0 37.0 35.0 37.0 5 36.20562464441945 37.0 37.0 37.0 35.0 37.0 6 36.247626903559485 37.0 37.0 37.0 35.0 37.0 7 36.21242254011387 37.0 37.0 37.0 35.0 37.0 8 36.20887538800023 37.0 37.0 37.0 35.0 37.0 9 37.90766240433147 39.0 38.0 39.0 35.0 39.0 10-11 37.89753308148359 39.0 38.0 39.0 35.0 39.0 12-13 37.84307386373885 39.0 38.0 39.0 35.0 39.0 14-15 39.13964620131502 40.5 38.5 41.0 36.0 41.0 16-17 39.098105861722495 40.0 38.0 41.0 36.0 41.0 18-19 39.1448591888839 40.0 38.5 41.0 36.0 41.0 20-21 39.09590229828741 40.0 39.0 41.0 36.0 41.0 22-23 38.95065041684887 40.0 38.0 41.0 35.0 41.0 24-25 38.90630150890469 40.0 38.0 41.0 35.0 41.0 26-27 38.873974940803606 40.0 38.0 41.0 35.0 41.0 28-29 38.81014667834751 40.0 38.0 41.0 35.0 41.0 30-31 38.60211932100995 40.0 38.0 41.0 35.0 41.0 32-33 38.411120585133844 40.0 38.0 41.0 34.0 41.0 34-35 38.20722027785375 40.0 38.0 41.0 34.0 41.0 36-37 38.0836113382114 40.0 37.5 41.0 34.0 41.0 38-39 38.07202897978426 40.0 38.0 41.0 34.0 41.0 40-41 38.000965521544956 40.0 38.0 41.0 33.5 41.0 42-43 37.836868541353134 40.0 37.0 41.0 33.0 41.0 44-45 37.59810439918039 40.0 37.0 41.0 33.0 41.0 46-47 37.21645696250481 40.0 37.0 41.0 32.5 41.0 48-49 36.844727998857266 40.0 36.0 41.0 31.0 41.0 50-51 36.55011181134377 40.0 36.0 41.0 31.0 41.0 52-53 36.14744325702276 39.0 35.0 41.0 30.0 41.0 54-55 35.930953387320635 39.0 35.0 41.0 29.0 41.0 56-57 35.71281572018255 39.0 35.0 40.5 29.0 41.0 58-59 35.395739029837316 38.5 35.0 40.0 28.0 41.0 60-61 35.0582355014545 38.0 34.5 40.0 27.5 41.0 62-63 34.715782486838336 37.5 34.0 40.0 26.5 41.0 64-65 34.338311826651704 37.0 34.0 40.0 26.0 41.0 66-67 33.93496050892565 36.5 34.0 39.0 26.0 41.0 68-69 33.49699959487584 36.0 33.0 39.0 25.5 40.5 70-71 32.998106983004774 35.0 33.0 38.5 24.0 40.0 72-73 32.58880409394786 35.0 32.5 37.5 23.0 40.0 74-75 32.14239285538432 35.0 32.0 37.0 22.0 39.0 76-77 30.828497925384028 34.0 30.5 35.5 20.5 38.5 78-79 31.547931404825313 35.0 32.0 36.0 22.0 38.5 80-81 31.37524905873228 35.0 32.0 36.0 20.5 37.0 82-83 31.024531706206492 35.0 31.5 35.0 19.5 37.0 84-85 30.658016217641865 34.0 31.0 35.0 18.0 36.5 86-87 30.226935832915288 34.0 31.0 35.0 13.5 36.0 88-89 29.798081681026392 34.0 30.0 35.0 7.5 36.0 90-91 29.478939393717795 34.0 30.0 35.0 4.5 35.0 92-93 29.08899324939317 34.0 29.5 35.0 2.0 35.0 94-95 28.706634529741528 34.0 29.0 35.0 2.0 35.0 96-97 28.155366822622405 34.0 29.0 35.0 2.0 35.0 98-99 27.507259571484916 34.0 28.0 35.0 2.0 35.0 100-101 25.1372915085293 31.0 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10473425207875664 1101 2 0.09555026537685052 1101 3 0.09239267095394865 1101 4 0.00490801745738878 1101 5 0.07034299191031579 1101 6 0.1287302754829085 1101 7 0.13422815039539415 1101 8 0.09763789410230572 1101 9 0.061481078100626974 1101 10-11 0.15540820468218186 1101 12-13 0.19988964015125532 1101 14-15 0.23494525287301826 1101 16-17 0.20787076834223228 1101 18-19 0.2854205293133347 1101 20-21 0.22304329086049535 1101 22-23 0.2761900420003869 1101 24-25 0.09169587350494623 1101 26-27 0.18968270732543147 1101 28-29 0.17281045592113742 1101 30-31 0.09931195654638003 1101 32-33 0.18161845915374641 1101 34-35 0.08904311611250648 1101 36-37 0.21744157009326415 1101 38-39 0.18870896386423652 1101 40-41 0.3175808595999783 1101 42-43 0.42050384237041527 1101 44-45 0.4358749123834187 1101 46-47 0.418886688198171 1101 48-49 0.35743944752768897 1101 50-51 0.2737793359927778 1101 52-53 0.14667457482669732 1101 54-55 0.24583926683690294 1101 56-57 0.2605012278985228 1101 58-59 0.1352362775718703 1101 60-61 0.18893991396283383 1101 62-63 0.1997998859942669 1101 64-65 0.024777038962007225 1101 66-67 -0.022729831000560807 1101 68-69 0.13358179521833335 1101 70-71 0.18855001531152737 1101 72-73 0.09332744164876772 1101 74-75 0.15390038263129924 1101 76-77 -0.00795174431025103 1101 78-79 -0.01361934697946765 1101 80-81 0.10862263737111988 1101 82-83 0.1515864299991172 1101 84-85 0.31283259439756606 1101 86-87 0.4243854658304116 1101 88-89 0.3663518395866525 1101 90-91 0.4220496819662678 1101 92-93 0.5199867268302008 1101 94-95 0.4735361124232007 1101 96-97 0.49050244118514286 1101 98-99 0.5483952449341096 1101 100-101 0.8485667301049595 1102 1 0.10862343367341509 1102 2 0.10192422627135755 1102 3 0.11901982234137876 1102 4 0.017796730722814402 1102 5 -0.0350050352527802 1102 6 0.06781820429686292 1102 7 0.07446933995165494 1102 8 0.01188739650248749 1102 9 0.044978619372137985 1102 10-11 0.08213487603213565 1102 12-13 0.0864169788703748 1102 14-15 0.08111768584339529 1102 16-17 0.1530263414601336 1102 18-19 0.07855804248509202 1102 20-21 0.12336029121174619 1102 22-23 0.18915237076750913 1102 24-25 0.09341303191731498 1102 26-27 -0.06244160481600147 1102 28-29 0.027902219180717225 1102 30-31 -0.06125559994583796 1102 32-33 0.017124986492468963 1102 34-35 0.06949066323813469 1102 36-37 0.01370313197126194 1102 38-39 0.01015083249787807 1102 40-41 0.11917054464856847 1102 42-43 0.08379853854182073 1102 44-45 0.15530223354019057 1102 46-47 0.11853280691963874 1102 48-49 0.07054212871256738 1102 50-51 -0.015293957532570346 1102 52-53 -0.04988671776198572 1102 54-55 -0.09152155156843378 1102 56-57 -0.08971288637381036 1102 58-59 -0.06523336883955722 1102 60-61 -0.140826653183872 1102 62-63 -0.3395154752046565 1102 64-65 -0.25869767125927723 1102 66-67 -0.3151219689235205 1102 68-69 -0.09723134141672318 1102 70-71 0.04360460493803231 1102 72-73 -0.05923870985574098 1102 74-75 -0.11915868539352203 1102 76-77 -0.11666208267812195 1102 78-79 -0.05250516245774861 1102 80-81 -0.003474306780621106 1102 82-83 0.07632738539075845 1102 84-85 -0.08937426077161703 1102 86-87 -0.19482598481939561 1102 88-89 -0.2523541081946341 1102 90-91 -0.07281684420162904 1102 92-93 0.025382172328388464 1102 94-95 0.0017375818061786674 1102 96-97 0.17232775438031922 1102 98-99 0.1605527791806871 1102 100-101 0.16078470809348744 1103 1 0.06124846016207641 1103 2 0.10987523990419135 1103 3 0.0953919350771244 1103 4 0.06738792235945823 1103 5 -9.09684774796915E-6 1103 6 0.09072648288695717 1103 7 0.09970420609560904 1103 8 0.056158781682967174 1103 9 0.024608837638716352 1103 10-11 0.054694828705727616 1103 12-13 0.0783931214146989 1103 14-15 0.17135573062689247 1103 16-17 0.1480442654553329 1103 18-19 0.18279755297460554 1103 20-21 0.16190791782755554 1103 22-23 0.22840856911461316 1103 24-25 0.1509132968039424 1103 26-27 0.09634026987495758 1103 28-29 0.171543615663019 1103 30-31 0.09450215240282489 1103 32-33 0.15726546470113334 1103 34-35 0.058316862291611926 1103 36-37 0.21243873979778272 1103 38-39 0.14809054430123325 1103 40-41 0.2399742989750493 1103 42-43 0.24165110264438994 1103 44-45 0.27361605355856256 1103 46-47 0.17190746256701317 1103 48-49 0.16789017208333235 1103 50-51 0.10490055754830507 1103 52-53 0.13384526994726542 1103 54-55 0.07675430136651329 1103 56-57 0.055667932302462475 1103 58-59 0.2120013913497516 1103 60-61 0.09152086041363816 1103 62-63 0.1340532540458952 1103 64-65 0.14846618760912378 1103 66-67 0.20424345101008612 1103 68-69 0.24581008685197503 1103 70-71 0.09855312863655286 1103 72-73 0.004225918949799734 1103 74-75 -0.031088296186773334 1103 76-77 0.07763667412063313 1103 78-79 0.002083556903698991 1103 80-81 0.01919336645523373 1103 82-83 0.15572715229052747 1103 84-85 0.08583058488776985 1103 86-87 0.13679220759425093 1103 88-89 0.08531453629219143 1103 90-91 0.153654601019813 1103 92-93 0.05866738459087273 1103 94-95 -0.07514355473405132 1103 96-97 -0.11745380244669335 1103 98-99 0.02160649818895166 1103 100-101 -0.01932936974786159 1104 1 0.15021703510743833 1104 2 0.11284829038691413 1104 3 0.10178129845391481 1104 4 0.008131012280500727 1104 5 0.032914781337247234 1104 6 0.09876538949936275 1104 7 0.06661076826329548 1104 8 0.044929118374000154 1104 9 0.11695761555326101 1104 10-11 0.1097941257658519 1104 12-13 0.1967617168746898 1104 14-15 0.12600977661314516 1104 16-17 0.18325667554685765 1104 18-19 0.18493615019298915 1104 20-21 0.17594290363478393 1104 22-23 0.2205923403723702 1104 24-25 0.1428660168756224 1104 26-27 0.10659156355226429 1104 28-29 0.2375190651007486 1104 30-31 0.08210650485244031 1104 32-33 0.12886527044020113 1104 34-35 0.27481208531487766 1104 36-37 0.2948321684194468 1104 38-39 0.22343311758867657 1104 40-41 0.3245146981310185 1104 42-43 0.35509461834854505 1104 44-45 0.38027901503293293 1104 46-47 0.3943053499794118 1104 48-49 0.4792657927553776 1104 50-51 0.6493626034005828 1104 52-53 0.5975686637359487 1104 54-55 0.4803088786396543 1104 56-57 0.6638778638924663 1104 58-59 0.7561960144249298 1104 60-61 0.6787744755927818 1104 62-63 0.614120575505865 1104 64-65 0.6724894926406435 1104 66-67 0.6854568700861705 1104 68-69 0.8030589711076317 1104 70-71 0.7115044503644725 1104 72-73 0.6828101691612218 1104 74-75 0.6777118495089312 1104 76-77 0.5734861260871078 1104 78-79 0.7369589933627907 1104 80-81 0.8125182113395581 1104 82-83 0.7814409774724389 1104 84-85 0.8940829587228372 1104 86-87 0.7245976766255424 1104 88-89 0.5810750217770497 1104 90-91 0.5728167774958202 1104 92-93 0.7042377434711931 1104 94-95 0.8156396224369438 1104 96-97 0.9329205894412382 1104 98-99 0.7271054640844348 1104 100-101 0.8532913002011426 1105 1 0.058986639370857574 1105 2 0.06303470489540786 1105 3 0.039173864566215855 1105 4 0.05274928365143694 1105 5 0.04381049567794548 1105 6 0.022425661741024783 1105 7 7.803802242136726E-4 1105 8 0.06782718795042086 1105 9 0.021401873391596382 1105 10-11 -0.047904201127067836 1105 12-13 -0.007019281191439575 1105 14-15 0.15977944335628536 1105 16-17 0.02178619771691359 1105 18-19 0.04278852709668257 1105 20-21 0.10139796846624449 1105 22-23 0.07064287397421509 1105 24-25 0.04661649984389271 1105 26-27 0.12631691267647227 1105 28-29 0.03566006834519442 1105 30-31 0.10827438837424097 1105 32-33 0.08902045008440496 1105 34-35 0.20337051810343354 1105 36-37 0.16093874183637524 1105 38-39 0.10294459111551646 1105 40-41 0.10808805357717688 1105 42-43 0.011739287153517353 1105 44-45 0.04791686655011773 1105 46-47 0.08873713186618915 1105 48-49 0.2237758242501542 1105 50-51 0.31978767546470266 1105 52-53 0.34114524813430336 1105 54-55 0.315720662515794 1105 56-57 0.21448031839597803 1105 58-59 0.23153839082379335 1105 60-61 0.2151053112366199 1105 62-63 0.24778645092928997 1105 64-65 0.14082464288439667 1105 66-67 0.20149491574035494 1105 68-69 0.2243867502433048 1105 70-71 0.3142127465223652 1105 72-73 0.36780838723945664 1105 74-75 0.3840261505182454 1105 76-77 0.2548550218804202 1105 78-79 0.28731340335674815 1105 80-81 0.2638590012532305 1105 82-83 0.39323107658527334 1105 84-85 0.5107079889005419 1105 86-87 0.41077563687744245 1105 88-89 0.3306010505807002 1105 90-91 0.1318733636336198 1105 92-93 0.24667384795528946 1105 94-95 0.038869184444070726 1105 96-97 0.24666290505982147 1105 98-99 0.3208574165758513 1105 100-101 0.24236592048686134 1106 1 -0.16590273089286 1106 2 -0.010840608418604347 1106 3 0.03349961616608965 1106 4 -0.007539155909867645 1106 5 -0.0634510025356434 1106 6 -0.06961017243632739 1106 7 -0.0034800428105654646 1106 8 -0.08111637631412094 1106 9 -0.1706694803000275 1106 10-11 -0.07095842846491252 1106 12-13 -0.12604598894018437 1106 14-15 -0.14869470690349118 1106 16-17 -0.09323551091802074 1106 18-19 -0.10076163516345815 1106 20-21 -0.054563278088828326 1106 22-23 -0.0512006919451764 1106 24-25 -0.0728044960983496 1106 26-27 -0.060517411214007666 1106 28-29 -0.04078015682118519 1106 30-31 -0.12298690933756973 1106 32-33 -0.09881134618851917 1106 34-35 -0.16743666225870157 1106 36-37 -0.21160112408166043 1106 38-39 -0.17054703469731436 1106 40-41 -0.11298206952276502 1106 42-43 -0.19288645204690624 1106 44-45 -0.006980689270804419 1106 46-47 0.01667532839103103 1106 48-49 0.027014503634134712 1106 50-51 -0.06517418722563662 1106 52-53 -0.21300390360644883 1106 54-55 -0.4613169188571149 1106 56-57 -0.40650391990950396 1106 58-59 -0.27470558482915663 1106 60-61 -0.3160812300093099 1106 62-63 -0.2602102364742791 1106 64-65 -0.3815076821040506 1106 66-67 -0.4148012743180658 1106 68-69 -0.5134570497547344 1106 70-71 -0.49815931667215096 1106 72-73 -0.3261854811474265 1106 74-75 -0.3766765314808893 1106 76-77 -0.32370630108598064 1106 78-79 -0.286732072653912 1106 80-81 -0.407778558678455 1106 82-83 -0.5809646318611534 1106 84-85 -0.5526143592055668 1106 86-87 -0.4063379576950048 1106 88-89 -0.5065364603047975 1106 90-91 -0.46285305576190794 1106 92-93 -0.34733954297353975 1106 94-95 -0.44768244632374277 1106 96-97 -0.2786412112945271 1106 98-99 -0.4649637497449355 1106 100-101 -0.31604485346201017 1107 1 0.044294885877121715 1107 2 0.0947321352083037 1107 3 0.060555034441108546 1107 4 0.07949421782352317 1107 5 0.03579088030119948 1107 6 -0.011513396002477805 1107 7 0.01725952467732128 1107 8 0.003804171483778873 1107 9 -0.10527985870891854 1107 10-11 -0.0025420509835001326 1107 12-13 0.020733021250933348 1107 14-15 0.062114623493556564 1107 16-17 0.08950892249917075 1107 18-19 0.014241310829653742 1107 20-21 -0.016384775549447284 1107 22-23 -0.015023896513596924 1107 24-25 0.001250825843818859 1107 26-27 -0.02415164656636648 1107 28-29 -0.09161287593921941 1107 30-31 0.0481554639007129 1107 32-33 0.029451929262961585 1107 34-35 0.05163007400657449 1107 36-37 -0.020742887958626 1107 38-39 0.04070441869662744 1107 40-41 0.1449774886006736 1107 42-43 0.014721379142081048 1107 44-45 0.08300640660726799 1107 46-47 0.02657812181978869 1107 48-49 0.1041669268534875 1107 50-51 0.20703165592207284 1107 52-53 0.21679767311594844 1107 54-55 0.15212801123435327 1107 56-57 0.11784230485073266 1107 58-59 0.08835603011274173 1107 60-61 0.17098776447731723 1107 62-63 0.293412662965423 1107 64-65 0.18676049960211571 1107 66-67 0.1019535854504241 1107 68-69 0.29027013560088477 1107 70-71 0.13637780396123134 1107 72-73 0.02866106957629455 1107 74-75 0.10152300746559462 1107 76-77 0.010947315092561638 1107 78-79 0.14490094845642787 1107 80-81 0.24140339616160134 1107 82-83 0.23328266129764685 1107 84-85 0.3599962835586048 1107 86-87 0.4200416744031017 1107 88-89 0.36773874697886555 1107 90-91 0.273818509691786 1107 92-93 0.3091679653883759 1107 94-95 0.15690837427535698 1107 96-97 0.34559176635400846 1107 98-99 0.5002417192728537 1107 100-101 0.5336472174479319 1108 1 0.025209223273222392 1108 2 0.03768187464583406 1108 3 0.022383709233302795 1108 4 -0.022461077408827634 1108 5 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-0.6908512026190543 2316 90-91 -0.5966274590567267 2316 92-93 -0.7430640745233426 2316 94-95 -0.6564289609662026 2316 96-97 -0.49794033248824476 2316 98-99 -0.6701164027096986 2316 100-101 -0.6797431918813963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 23.0 5 40.0 6 85.0 7 192.0 8 426.0 9 802.0 10 1428.0 11 2356.0 12 3503.0 13 5221.0 14 7117.0 15 9767.0 16 13070.0 17 19997.0 18 20093.0 19 12465.0 20 9870.0 21 10156.0 22 10092.0 23 10967.0 24 12340.0 25 14555.0 26 17334.0 27 21158.0 28 26102.0 29 31884.0 30 39173.0 31 48683.0 32 61527.0 33 81072.0 34 113879.0 35 180326.0 36 285404.0 37 480304.0 38 450044.0 39 49758.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71786977817624 18.981651434290665 15.206489006802284 22.093989780730812 2 15.766980141704956 20.24910270269216 40.0864311483699 23.89748600723299 3 19.166480586382043 24.803786871930285 30.166555668558022 25.86317687312965 4 12.946715203563922 17.188379810483795 36.27099540128012 33.59390958467217 5 13.75701227999101 37.434496395564985 33.42669227090374 15.381799053540254 6 31.79693285420454 35.83613288267536 17.116032727792152 15.250901535327948 7 28.708141435621577 33.58630436573693 19.766061515495878 17.939492683145616 8 26.977922385626492 34.723808000904825 19.015726266052656 19.282543347416023 9 27.03601180958027 16.739534012995218 19.9160887980593 36.30836537936521 10-11 25.52706361034368 26.853540546298476 26.072372433421425 21.547023409936415 12-13 26.43486349363282 24.339284417150157 27.22495311333775 22.000898975879267 14-15 23.130956507410456 25.28281907915424 26.16656014485017 25.41966426858513 16-17 22.008005481617154 28.957578650163963 26.62718446270621 22.407231405512672 18-19 21.779006963162953 26.558107041506457 29.935263011383938 21.727622983946652 20-21 22.78901416374524 25.60116574355884 30.128464823181094 21.48135526951482 22-23 22.556738102098116 25.76996747793877 30.048268764537063 21.625025655426057 24-25 22.231203817643927 25.98797820495069 30.15119145359484 21.629626523810543 26-27 21.973106415514245 26.376222000681203 29.979156777168175 21.67151480663638 28-29 21.598350056295722 26.39685670221378 30.378727992076914 21.626065249413582 30-31 21.85474226839305 25.91361348814829 30.34811427133959 21.883529972119074 32-33 21.627903348708006 26.17578218504307 30.216496709989084 21.979817756259845 34-35 22.215907705660634 26.143878332187455 29.837797379597532 21.80241658255438 36-37 22.313455884485897 25.884561465348476 29.81700207713405 21.984980573031578 38-39 21.853277278472124 26.029013364227872 30.06032138900591 22.05738796829409 40-41 21.9548591726132 26.0312592184464 30.082475382938835 21.931406226001567 42-43 22.186194411710357 25.908819871294497 29.77483607088895 22.130149646106194 44-45 21.92298973017641 26.243311695118116 29.56258790778854 22.271110666916933 46-47 22.113146267515788 26.575902840308284 29.115317169627257 22.195633722548667 48-49 22.339867483935194 26.52870994523755 28.87392058298878 22.257501987838474 50-51 22.221742833421818 26.737999720166943 28.69873241475939 22.341525031651848 52-53 22.479589006168517 26.778439009312983 28.54582363789798 22.19614834662053 54-55 22.119169880602936 26.684714436216833 28.549821252979317 22.646294430200907 56-57 22.207263666602802 27.096322535384985 28.466212596095108 22.230201201917097 58-59 22.446706184554287 26.64610332469946 28.422263205902038 22.48492728484421 60-61 22.253211864336546 26.67708484157988 28.84971551118528 22.2199877828983 62-63 22.20180350941853 26.72356930475136 28.761499846676834 22.313127339153276 64-65 22.047432190454185 26.663410072917475 28.679304980492127 22.609852756136217 66-67 22.022812731721515 26.95355405043723 28.458095487423847 22.565537730417415 68-69 21.866479915971937 26.836888982383407 28.69027424905038 22.606356852594274 70-71 21.93727839440178 26.94955643535588 28.435718593249003 22.677446576993336 72-73 22.154368393880137 26.955187222452164 28.209048942996446 22.68139544067125 74-75 21.983007210820084 27.195434138560266 28.031569458805794 22.78998919181386 76-77 22.05781623938499 27.15592112607942 28.101795855448188 22.684466779087405 78-79 22.092064100295286 27.16416011325926 27.994177132374197 22.749598654071253 80-81 21.861981851802557 27.55378132948002 28.086731671788 22.497505146929416 82-83 22.160399995709877 27.366028640488448 27.89883298833573 22.57473837546594 84-85 22.06528365826791 27.137934136181226 27.845413063313305 22.951369142237564 86-87 22.172455055373185 26.90805489517572 28.046841426205937 22.87264862324516 88-89 22.13851060489796 27.13991952114398 27.865584514986676 22.855985358971385 90-91 21.982202286104577 27.255433782407874 27.973982293938942 22.788381637548607 92-93 22.09804381840913 27.0005759999376 27.850827960299636 23.05055222135363 94-95 21.929624424063107 26.948776412900987 27.92816773212859 23.193431430907317 96-97 22.08850524784482 26.971348312689553 27.74296115049412 23.197185288971507 98-99 21.953683241656318 27.07972268251672 27.62673780471455 23.339856271112406 100-101 21.870927734332152 26.979636051272827 27.894090501130304 23.25534571326472 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2822.0 1 1838.5 2 1173.0 3 1555.0 4 1194.5 5 1017.0 6 2212.0 7 5115.0 8 8040.5 9 8903.0 10 6882.0 11 4383.0 12 3843.0 13 3889.0 14 3901.5 15 3891.5 16 3910.0 17 3975.0 18 4132.0 19 4317.0 20 4535.0 21 4927.5 22 5726.5 23 6724.5 24 7785.5 25 9299.5 26 11097.5 27 13323.5 28 15276.0 29 17389.0 30 20204.5 31 22957.5 32 25923.5 33 29048.5 34 32490.0 35 35639.0 36 39529.5 37 43630.5 38 46957.5 39 49213.0 40 51845.5 41 57882.5 42 63991.5 43 68692.0 44 73815.0 45 79253.0 46 80368.5 47 80585.5 48 84660.0 49 84625.0 50 80918.0 51 76535.0 52 71772.0 53 70678.0 54 70684.0 55 66283.0 56 58706.0 57 52582.0 58 47798.0 59 42110.5 60 35940.5 61 29585.5 62 24043.5 63 19034.5 64 15241.0 65 12296.5 66 8966.0 67 7284.5 68 5449.5 69 3247.5 70 2259.5 71 1723.0 72 1416.5 73 1143.0 74 861.0 75 714.5 76 503.5 77 233.0 78 103.5 79 64.5 80 31.0 81 15.5 82 8.5 83 2.5 84 1.0 85 3.0 86 2.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016672979973410985 3 0.006678942270050598 4 0.0 5 0.0 6 0.0 7 0.0 8 2.4375701715513134E-4 9 3.412598240171839E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.7062991200859196E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005606411394568021 26-27 0.021279987597642967 28-29 7.312710514653942E-5 30-31 0.0 32-33 6.825196480343678E-4 34-35 0.019159301548393324 36-37 0.003875736572766589 38-39 0.01828177628663485 40-41 0.01501543225675609 42-43 0.008970258231308833 44-45 0.003315095433309786 46-47 9.750280686205254E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.7062991200859196E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.875140343102627E-5 84-85 0.003241968328163247 86-87 2.193813154396182E-4 88-89 4.875140343102627E-5 90-91 2.193813154396182E-4 92-93 3.65635525732697E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2051223.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.99005284768918 #Duplication Level Percentage of deduplicated Percentage of total 1 73.47247509817404 35.259479628118996 2 14.2661104485591 13.692627887146442 3 4.999271492287255 7.1974590934443405 4 2.2796461774760886 4.376013621244405 5 1.2929215462125085 3.102368666532715 6 0.7868926526012523 2.2657811990275456 7 0.5356925108240541 1.7995538333190366 8 0.3457613747528779 1.327448531766422 9 0.26411631319227397 1.140746024523065 >10 1.5309012239784414 13.442128422673042 >50 0.1337935874030091 4.465339657699115 >100 0.08682078873251435 7.608218720227618 >500 0.0035615909678831403 1.0859828103942315 >1k 0.0017299156129718107 1.3330856533025328 >5k 1.0175974193951829E-4 0.4397551581796948 >10k+ 2.0351948387903657E-4 1.4640110924007852 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16039 0.7819237596302304 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13940 0.6795945638285062 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9005 0.4390063878963916 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3536 0.1723849625321089 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2529 0.12329229927706543 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2342 0.11417578683546353 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.875140343102627E-5 0.0 8 0.0 0.0 0.0 4.875140343102627E-5 0.0 9 0.0 0.0 0.0 9.750280686205254E-5 0.0 10-11 0.0 0.0 0.0 9.750280686205254E-5 0.0 12-13 0.0 0.0 0.0 9.750280686205254E-5 0.0 14-15 0.0 0.0 0.0 9.750280686205254E-5 0.0 16-17 0.0 0.0 0.0 9.750280686205254E-5 0.0 18-19 0.0 0.0 0.0 1.462542102930788E-4 0.0 20-21 0.0 0.0 0.0 2.4375701715513136E-4 0.0 22-23 0.0 0.0 0.0 5.850168411723152E-4 0.0 24-25 0.0 0.0 0.0 9.019009634739861E-4 0.0 26-27 0.0 0.0 0.0 0.001974431838956564 0.0 28-29 0.0 0.0 0.0 0.005411405780843916 0.0 30-31 0.0 0.0 0.0 0.01584420611508354 0.0 32-33 0.0 0.0 0.0 0.035637275908080204 0.0 34-35 0.0 0.0 0.0 0.06308431603974798 0.0 36-37 0.0 0.0 0.0 0.1059611753573356 0.0 38-39 0.0 0.0 0.0 0.17228745972524684 0.0 40-41 0.0 0.0 0.0 0.24297699470023493 0.0 42-43 0.0 0.0 0.0 0.31035143424191325 0.0 44-45 0.0 0.0 0.0 0.39257067612833907 0.0 46-47 0.0 0.0 0.0 0.4925841802670895 0.0 48-49 0.0 0.0 0.0 0.594840248963667 0.0 50-51 0.0 0.0 0.0 0.7062615815052776 0.0 52-53 0.0 0.0 0.0 0.8116864914248719 0.0 54-55 0.0 0.0 0.0 0.9239122221230944 0.0 56-57 0.0 0.0 0.0 1.0548828674405464 0.0 58-59 0.0 0.0 0.0 1.1995770328238322 0.0 60-61 0.0 0.0 0.0 1.352047047054367 0.0 62-63 0.0 0.0 0.0 1.5019576126047727 0.0 64-65 0.0 0.0 0.0 1.6553051520970659 0.0 66-67 0.0 0.0 0.0 1.8280313744531922 0.0 68-69 0.0 0.0 0.0 2.0187224889736513 0.0 70-71 0.0 0.0 0.0 2.221601454351867 0.0 72-73 0.0 0.0 0.0 2.4285999133200047 0.0 74-75 0.0 0.0 0.0 2.634672095622953 0.0 76-77 0.0 0.0 0.0 2.85590596439295 0.0 78-79 2.4375701715513136E-5 0.0 0.0 3.0943490785740995 0.0 80-81 4.875140343102627E-5 0.0 0.0 3.359166702011434 0.0 82-83 4.875140343102627E-5 0.0 0.0 3.622253650626967 0.0 84-85 4.875140343102627E-5 0.0 0.0 3.8882900591500777 0.0 86-87 4.875140343102627E-5 0.0 0.0 4.165417411953747 0.0 88-89 4.875140343102627E-5 0.0 0.0 4.461679690604093 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6930 0.0 54.366253 1 GTATCAA 11570 0.0 39.215656 1 TTCGCGA 30 0.0039497428 31.670568 22-23 TATCAAC 14600 0.0 31.07782 2 ATCAACG 14755 0.0 30.682463 3 TCAACGC 14915 0.0 30.444414 4 CAACGCA 15110 0.0 29.98865 5 GTGGTAT 2905 0.0 29.438509 1 AACGCAG 15670 0.0 28.947254 6 TGGTATC 3275 0.0 25.678047 2 ACGCAGA 17655 0.0 25.558119 7 CGCAGAG 17595 0.0 25.537292 8 GAGTACT 10405 0.0 23.805933 12-13 GCAGAGT 18850 0.0 23.458527 9 GTACATG 11595 0.0 23.355736 1 ACCGTCG 105 0.0021974114 22.617973 8 TACATGG 11975 0.0 22.416765 2 GTACTTT 10965 0.0 22.351881 14-15 CAGAGTA 18345 0.0 21.722607 10-11 AGTACTT 10665 0.0 21.68908 12-13 >>END_MODULE