FastQCFastQC Report
Thu 26 May 2016
SRR938140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026137
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153130.7557731782204263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132690.6548915497816782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83940.4142859046550159No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31990.15788665820721898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25590.12629945556494945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.11479973960299822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64500.054.989891
GTATCAA112550.041.2002451
TATCAAC144500.031.9287222
ATCAACG146500.031.4302353
TCAACGC147900.031.1648834
CAACGCA150200.030.5926485
AACGCAG153500.029.9607526
GTGGTAT27150.028.5827561
ACGCAGA172300.026.5201557
CGCAGAG173050.026.237118
TGGTATC28500.026.201882
GTACATG111050.025.4227451
GAGTACT102700.024.8546412-13
GCAGAGT182000.024.5027969
TACATGG113600.024.3262652
ACATGGG116300.023.2315673
GTACTTT109950.023.02006514-15
AGTACTT105500.022.16747712-13
CAGAGTA178350.021.8424310-11
GTACCTG17400.021.3418311
Produced by FastQC (version 0.11.5)