##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938140_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2026137 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.919592307923896 33.0 31.0 34.0 30.0 34.0 2 32.02881739981058 33.0 31.0 34.0 30.0 34.0 3 32.038098114786905 34.0 31.0 34.0 30.0 34.0 4 35.56776614809364 37.0 35.0 37.0 33.0 37.0 5 35.57848013238986 37.0 35.0 37.0 33.0 37.0 6 35.612898831618985 37.0 35.0 37.0 33.0 37.0 7 35.5487926038565 37.0 35.0 37.0 33.0 37.0 8 35.54752319315031 37.0 35.0 37.0 33.0 37.0 9 37.15985246802166 39.0 37.0 39.0 34.0 39.0 10-11 37.061794686144125 39.0 37.0 39.0 33.0 39.0 12-13 36.89422334225178 39.0 37.0 39.0 33.0 39.0 14-15 38.03105688312291 40.0 38.0 41.0 33.0 41.0 16-17 37.85354544139908 40.0 38.0 41.0 33.0 41.0 18-19 38.06090185411944 40.0 38.0 41.0 33.0 41.0 20-21 38.08580342790246 40.0 38.0 41.0 33.0 41.0 22-23 37.9634703872443 40.0 38.0 41.0 33.0 41.0 24-25 37.82884869088319 40.0 38.0 41.0 32.5 41.0 26-27 37.64001767896248 40.0 37.5 41.0 32.0 41.0 28-29 37.45065412654722 40.0 37.0 41.0 32.0 41.0 30-31 37.20537382220452 40.0 37.0 41.0 31.5 41.0 32-33 36.89518897290756 39.5 36.0 41.0 30.5 41.0 34-35 36.63411210594347 39.0 35.5 41.0 30.0 41.0 36-37 36.36403856205183 39.0 35.0 41.0 30.0 41.0 38-39 36.06825451585949 39.0 35.0 40.0 29.5 41.0 40-41 35.92932980346344 39.0 35.0 40.0 29.0 41.0 42-43 35.68509409778312 39.0 35.0 40.0 27.5 41.0 44-45 35.302945457291386 38.0 35.0 40.0 26.5 41.0 46-47 34.840000947616076 38.0 34.5 40.0 24.5 41.0 48-49 34.383424467348455 38.0 34.0 40.0 23.0 41.0 50-51 33.63982494767136 37.5 33.0 39.5 22.0 40.5 52-53 33.70853007471854 38.0 33.0 39.5 22.0 40.5 54-55 33.77702371557304 38.0 33.0 40.0 20.5 41.0 56-57 33.46369742026329 38.0 33.0 40.0 18.5 41.0 58-59 33.04537131497031 37.0 32.5 40.0 15.0 41.0 60-61 32.54003974064932 36.5 32.0 40.0 10.0 41.0 62-63 31.98867031202727 36.0 31.0 39.0 7.5 41.0 64-65 31.43069446932759 35.0 30.5 39.0 3.5 40.0 66-67 30.85136666474182 35.0 29.5 38.5 2.0 40.0 68-69 30.166375225367286 35.0 29.0 38.0 2.0 40.0 70-71 29.567576624877784 34.0 28.0 37.0 2.0 39.5 72-73 28.8570101626889 34.0 27.0 36.5 2.0 39.0 74-75 28.153344023627227 34.0 26.0 36.0 2.0 39.0 76-77 27.368615004809648 33.0 25.5 35.5 2.0 38.0 78-79 26.5892982557448 32.5 23.5 35.0 2.0 37.0 80-81 25.826572684867806 32.0 20.5 35.0 2.0 36.5 82-83 25.0642271475226 31.5 18.5 35.0 2.0 36.0 84-85 24.29223813592072 31.0 10.5 34.0 2.0 35.5 86-87 23.492929402108544 30.5 4.5 34.0 2.0 35.0 88-89 22.593216796297583 29.5 2.0 34.0 2.0 35.0 90-91 21.666880867384585 29.0 2.0 34.0 2.0 35.0 92-93 20.68928705215886 28.5 2.0 34.0 2.0 35.0 94-95 19.569361055052052 26.5 2.0 33.5 2.0 35.0 96-97 18.277009896171876 24.5 2.0 33.0 2.0 35.0 98-99 16.703567182278395 11.0 2.0 32.0 2.0 34.0 100-101 12.928567268649651 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19117881481015786 1101 2 0.19542737645310382 1101 3 0.13490539988554673 1101 4 0.009496730109638918 1101 5 0.10021392554771325 1101 6 0.14745162753304442 1101 7 0.24555635868534864 1101 8 0.2989350529308439 1101 9 0.18719478754793073 1101 10-11 0.2975933660125847 1101 12-13 0.3875822897400951 1101 14-15 0.3439179619503463 1101 16-17 0.3467611252180163 1101 18-19 0.38021556922167576 1101 20-21 0.3129610100564335 1101 22-23 0.35180698634753327 1101 24-25 0.2602462445255611 1101 26-27 0.32062298291577207 1101 28-29 0.40160003281686585 1101 30-31 0.23896675916972754 1101 32-33 0.22948730589286725 1101 34-35 0.2976533963739243 1101 36-37 0.3236232642740191 1101 38-39 0.20533716262716695 1101 40-41 0.5596966419494791 1101 42-43 0.45755048582007873 1101 44-45 0.39922244987578637 1101 46-47 0.5231980694485969 1101 48-49 0.5218421948242593 1101 50-51 0.45452531038917954 1101 52-53 0.3067450273369161 1101 54-55 0.3819081834125626 1101 56-57 0.36761465789254544 1101 58-59 0.476246475488999 1101 60-61 0.2817828667436615 1101 62-63 0.18393453348613065 1101 64-65 0.23567652770486447 1101 66-67 0.3468114409955767 1101 68-69 0.2686938747506318 1101 70-71 0.11012895712468307 1101 72-73 0.1661259817160321 1101 74-75 0.16687061078612686 1101 76-77 0.08808638324947182 1101 78-79 -0.13710624494159163 1101 80-81 -0.03459453018395209 1101 82-83 -0.29974777437745104 1101 84-85 -0.48380116072529233 1101 86-87 -0.30469952006750844 1101 88-89 -0.36714955890298384 1101 90-91 -0.34685321379728506 1101 92-93 -0.6779317909336982 1101 94-95 -1.095268617672538 1101 96-97 -0.8449025704394977 1101 98-99 -1.0430009311967918 1101 100-101 -1.2877317631811618 1102 1 0.22173217283229718 1102 2 0.22917703089893848 1102 3 0.21351594728156442 1102 4 0.13932509319566577 1102 5 0.043799739545939076 1102 6 0.23143880667723948 1102 7 0.17536968936889963 1102 8 0.2775259817677025 1102 9 0.2976217969533721 1102 10-11 0.21618749241980595 1102 12-13 0.27737089339551346 1102 14-15 0.3923172021770469 1102 16-17 0.29516845491554733 1102 18-19 0.33032869441993284 1102 20-21 0.38980811651004643 1102 22-23 0.4241475568909081 1102 24-25 0.3195019367740599 1102 26-27 0.3190445806563247 1102 28-29 0.13693484136393153 1102 30-31 0.20000990598661872 1102 32-33 0.17833382378970697 1102 34-35 0.18074525581841527 1102 36-37 0.3088443743532494 1102 38-39 0.20583845503159637 1102 40-41 0.21765916540577024 1102 42-43 0.4081031177007475 1102 44-45 0.46485035205305536 1102 46-47 0.19726405405184977 1102 48-49 0.2045591359596628 1102 50-51 0.2801956217764783 1102 52-53 0.30888236744246456 1102 54-55 0.4653816493113041 1102 56-57 0.4105426768894205 1102 58-59 0.32878179208031355 1102 60-61 0.4338674660473245 1102 62-63 0.37826750884445204 1102 64-65 0.36479945071731024 1102 66-67 0.28052396031157656 1102 68-69 0.19777628671234737 1102 70-71 -0.27764715180163435 1102 72-73 -0.1377893858580599 1102 74-75 -0.03318327655493647 1102 76-77 -0.06768433949060437 1102 78-79 -0.1601994547544372 1102 80-81 -0.16708541201270322 1102 82-83 -0.32246995137507284 1102 84-85 -0.2459751773450165 1102 86-87 -0.12116152949205627 1102 88-89 -0.2928251571097995 1102 90-91 -0.4992037464921246 1102 92-93 -0.7610042866055124 1102 94-95 -0.5748987568751325 1102 96-97 -0.6925921666156576 1102 98-99 -0.7195192356946656 1102 100-101 -0.6213838981007953 1103 1 0.22698111344245575 1103 2 0.16490616117813062 1103 3 0.2840264113281492 1103 4 0.09368948672355515 1103 5 0.2406572640333735 1103 6 0.12432688841196438 1103 7 0.1534939216122737 1103 8 0.2132499301659223 1103 9 0.15160797413346927 1103 10-11 0.2590910283524863 1103 12-13 0.20301303513398494 1103 14-15 0.2718796197622808 1103 16-17 0.2897102577589905 1103 18-19 0.23077302547262946 1103 20-21 0.2753152184303289 1103 22-23 0.21246589020289974 1103 24-25 0.3102163108851457 1103 26-27 0.12575051378811253 1103 28-29 0.2735979599662173 1103 30-31 0.1845325419667887 1103 32-33 0.10267348450452829 1103 34-35 0.06470678553406373 1103 36-37 -0.07242623505423751 1103 38-39 -0.044593012763620266 1103 40-41 0.01759039879707558 1103 42-43 0.06304745515458876 1103 44-45 0.2599222003568471 1103 46-47 0.12947621123232267 1103 48-49 0.2504550557465848 1103 50-51 0.24053060904429202 1103 52-53 0.3142515581728702 1103 54-55 0.3179409803243729 1103 56-57 0.3480008789230453 1103 58-59 0.37193405829975745 1103 60-61 0.1997395674707647 1103 62-63 0.1464360979889605 1103 64-65 0.03614276496302793 1103 66-67 0.038980942469446944 1103 68-69 0.03186236446128632 1103 70-71 -0.054356242832653834 1103 72-73 -0.3660331061803568 1103 74-75 -0.21203178335021278 1103 76-77 -0.34361081408627925 1103 78-79 -0.37477642123254284 1103 80-81 -0.4631605639180236 1103 82-83 -0.7305267703951337 1103 84-85 -0.963603814492032 1103 86-87 -0.8958969335050284 1103 88-89 -0.7508203727095797 1103 90-91 -0.9883280451855008 1103 92-93 -1.1732815952706552 1103 94-95 -1.202082081460233 1103 96-97 -1.514227556058401 1103 98-99 -1.834646609377172 1103 100-101 -1.7038203088008324 1104 1 0.20132368544268076 1104 2 0.12967043453699034 1104 3 0.13931481647666288 1104 4 0.08496069449164878 1104 5 0.20987347480206608 1104 6 0.28846610269825135 1104 7 0.29989057961306287 1104 8 0.27209960055515836 1104 9 0.271733988046698 1104 10-11 0.30428810017450303 1104 12-13 0.29793555468344834 1104 14-15 0.30298837935766443 1104 16-17 0.33842862474804036 1104 18-19 0.42130131369443546 1104 20-21 0.32031073176109004 1104 22-23 0.3737669161014594 1104 24-25 0.3529787383326237 1104 26-27 0.2846519118296911 1104 28-29 0.23140946178195776 1104 30-31 0.2600495053343934 1104 32-33 0.3675715892853404 1104 34-35 0.4113267521231805 1104 36-37 0.35656211782571745 1104 38-39 0.2836501146042565 1104 40-41 0.12613861397955617 1104 42-43 0.11772290043373346 1104 44-45 0.07460354435342254 1104 46-47 -0.015426266342871031 1104 48-49 0.07734093720058155 1104 50-51 -0.09161472487662792 1104 52-53 0.09560970369810207 1104 54-55 0.08857638492742836 1104 56-57 0.24265838321896638 1104 58-59 0.04437588817755511 1104 60-61 0.1495542076774683 1104 62-63 0.11373010122527916 1104 64-65 -0.02529912412969182 1104 66-67 -0.01590203973077564 1104 68-69 0.12163489374704994 1104 70-71 0.08595480063679517 1104 72-73 -0.09365570100110432 1104 74-75 -0.2954045458516781 1104 76-77 -0.21121079905480045 1104 78-79 -0.5500571512159063 1104 80-81 -0.49770820175499253 1104 82-83 -0.3989792794567286 1104 84-85 -0.2875836096767195 1104 86-87 -0.284850304029888 1104 88-89 -0.4264491327149038 1104 90-91 -0.7854890312050848 1104 92-93 -1.0395444047682716 1104 94-95 -1.4274838319444854 1104 96-97 -1.733112675689629 1104 98-99 -1.6492409814541489 1104 100-101 -1.5849000872459165 1105 1 0.2764319270768212 1105 2 0.2352732314817345 1105 3 0.3108805901697238 1105 4 0.1884583069722794 1105 5 0.2906801095927207 1105 6 0.1637138416331041 1105 7 0.2547718717048255 1105 8 0.11665236616470054 1105 9 0.2511275167716889 1105 10-11 0.3098206712831342 1105 12-13 0.39421374443159607 1105 14-15 0.2544693253306889 1105 16-17 0.3496898107773134 1105 18-19 0.32051252565079835 1105 20-21 0.20800228207396287 1105 22-23 0.3008040220653285 1105 24-25 0.2949613019653441 1105 26-27 0.3489554751347441 1105 28-29 0.3401135684425114 1105 30-31 0.21201837373610743 1105 32-33 0.21738731605513806 1105 34-35 0.3394840516335904 1105 36-37 0.34784592245950563 1105 38-39 0.10814596786009645 1105 40-41 0.3014817105333165 1105 42-43 0.3592381401559521 1105 44-45 0.5710489081757473 1105 46-47 0.5260407776186611 1105 48-49 0.4942585240404327 1105 50-51 0.5319549108397652 1105 52-53 0.5969923494067757 1105 54-55 0.5882483197247623 1105 56-57 0.5727110645256914 1105 58-59 0.6949327639470155 1105 60-61 0.7009323996524799 1105 62-63 0.5939571143924312 1105 64-65 0.4498486443906806 1105 66-67 0.5081866945499307 1105 68-69 0.3090794209036929 1105 70-71 0.24167522386874296 1105 72-73 0.02837699894767809 1105 74-75 0.11738538425651512 1105 76-77 -0.12335613074661822 1105 78-79 -0.10998690947360856 1105 80-81 -0.127948894246952 1105 82-83 -0.1406189851285582 1105 84-85 0.09215404950449368 1105 86-87 -2.2530840409018538E-4 1105 88-89 0.10796192463327614 1105 90-91 0.24122064580378932 1105 92-93 -0.1970912564557601 1105 94-95 -0.4475244404517511 1105 96-97 -0.913385498688708 1105 98-99 -0.8591860096558257 1105 100-101 -0.8512027407168041 1106 1 0.19633054821542828 1106 2 0.25016732748714077 1106 3 0.32923970201876784 1106 4 0.2097150918367845 1106 5 0.3256157823859027 1106 6 0.3117631009402473 1106 7 0.3236505799891063 1106 8 0.35730609480641107 1106 9 0.3968691282634822 1106 10-11 0.28480088091360756 1106 12-13 0.25306077678345673 1106 14-15 0.3884651540424855 1106 16-17 -0.03686104536881629 1106 18-19 0.28697663873603574 1106 20-21 0.4125045627864594 1106 22-23 0.4175311939591424 1106 24-25 0.3661055539120639 1106 26-27 0.3770725433928064 1106 28-29 0.3027229541811991 1106 30-31 0.23960396566662467 1106 32-33 0.26369635543399994 1106 34-35 0.16886228812453652 1106 36-37 0.16512013645861146 1106 38-39 0.291538190301587 1106 40-41 0.25893326665744354 1106 42-43 0.17224962764321816 1106 44-45 0.49644431862460436 1106 46-47 0.6136432168809378 1106 48-49 0.6438499011099168 1106 50-51 0.6025235975984771 1106 52-53 0.7860833760336163 1106 54-55 0.6379478066495139 1106 56-57 0.384261240200658 1106 58-59 0.37929565014275113 1106 60-61 0.5162090055077044 1106 62-63 0.35589725674699224 1106 64-65 0.13228905779959277 1106 66-67 0.16761484927092596 1106 68-69 0.18020583883546948 1106 70-71 -0.15529633924155561 1106 72-73 -0.17063685657921823 1106 74-75 0.005802432554091297 1106 76-77 -0.3683271653576483 1106 78-79 -0.36147132895232303 1106 80-81 -0.19597828946686846 1106 82-83 0.038934954686038026 1106 84-85 0.07779440688794637 1106 86-87 -0.0483255463421699 1106 88-89 -0.5192287672017493 1106 90-91 -0.45306818811814864 1106 92-93 -0.41976963062870354 1106 94-95 -0.49438352735018753 1106 96-97 -0.6763437960137786 1106 98-99 -0.9141675121815069 1106 100-101 -1.017874652681984 1107 1 0.039364276178687874 1107 2 0.012818433587959532 1107 3 -0.004772378149617396 1107 4 0.04269553228039058 1107 5 -0.029907857971181784 1107 6 -0.025134866563099934 1107 7 0.06901132110576924 1107 8 0.03647030023582687 1107 9 -0.029510065892772275 1107 10-11 0.03969180997174249 1107 12-13 -0.04454440471025123 1107 14-15 0.020065776354194043 1107 16-17 0.14526236453326646 1107 18-19 0.0853877887647343 1107 20-21 0.1577654678007292 1107 22-23 0.13552436350580876 1107 24-25 0.12508612331063063 1107 26-27 0.16764967926142305 1107 28-29 0.23955114040880687 1107 30-31 0.09629704036899511 1107 32-33 0.12621112285748382 1107 34-35 0.12172248846231781 1107 36-37 0.016610814262548956 1107 38-39 -0.04062848191153279 1107 40-41 0.13271963403766307 1107 42-43 -0.05043635869587604 1107 44-45 -0.07179157744601383 1107 46-47 0.13507470019583678 1107 48-49 0.04960254052687674 1107 50-51 0.0015274204892321563 1107 52-53 0.026217281205958898 1107 54-55 -0.0875169512063465 1107 56-57 -0.1282780702646562 1107 58-59 -0.07343195537153235 1107 60-61 -0.10797098650540704 1107 62-63 -0.1355984752596271 1107 64-65 -0.22927622669170233 1107 66-67 -0.11019158516587524 1107 68-69 -0.17172370183526198 1107 70-71 -0.11637169885951693 1107 72-73 -0.0894030279478919 1107 74-75 -0.4075133115992564 1107 76-77 -0.4655748088096452 1107 78-79 -0.5149802559638097 1107 80-81 -0.523483863649254 1107 82-83 -0.6389968988543977 1107 84-85 -0.6239327576835514 1107 86-87 -0.6918170644333088 1107 88-89 -0.6998546355678492 1107 90-91 -0.734504600840598 1107 92-93 -1.1104425467580548 1107 94-95 -1.3179299760968277 1107 96-97 -1.486151341127215 1107 98-99 -1.1741677617717627 1107 100-101 -1.051341066153201 1108 1 0.1129609991496423 1108 2 0.13726965838935712 1108 3 0.19239613973415715 1108 4 0.2497186336845303 1108 5 0.2516718229665216 1108 6 0.06296076454206201 1108 7 0.2067902142831457 1108 8 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-0.761734334356376 2316 54-55 -0.6871299294165993 2316 56-57 -0.707609424475379 2316 58-59 -0.6124547253524213 2316 60-61 -0.7716811849174832 2316 62-63 -0.6568176274670634 2316 64-65 -0.6924927447936255 2316 66-67 -0.7126499717445434 2316 68-69 -0.611472547968738 2316 70-71 -0.9356560032161987 2316 72-73 -0.7630247850178655 2316 74-75 -0.721541527337795 2316 76-77 -0.7990866212547694 2316 78-79 -0.5885592489164004 2316 80-81 -0.5211565482511133 2316 82-83 -0.766241875142974 2316 84-85 -1.0080416265847703 2316 86-87 -0.7901859108204192 2316 88-89 -0.8194332159856508 2316 90-91 -0.7652643660911416 2316 92-93 -0.6671861291564731 2316 94-95 -0.5729658099281103 2316 96-97 -0.6512972986219587 2316 98-99 -0.9302209451901025 2316 100-101 -0.5349045759962223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6682.0 3 2026.0 4 1999.0 5 2192.0 6 2588.0 7 3340.0 8 4162.0 9 5166.0 10 6671.0 11 8256.0 12 10373.0 13 12758.0 14 14775.0 15 18206.0 16 22536.0 17 28871.0 18 28372.0 19 22685.0 20 23459.0 21 24778.0 22 27119.0 23 29951.0 24 33502.0 25 38203.0 26 42925.0 27 48640.0 28 55795.0 29 64381.0 30 75283.0 31 89167.0 32 106665.0 33 131638.0 34 167767.0 35 217716.0 36 257413.0 37 261212.0 38 122746.0 39 6119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.40076827874719 18.580246181342254 14.828758456799642 22.190227083110916 2 15.578870854564911 20.003762797585686 40.56577266480634 23.851593683043067 3 19.155299950323393 24.82169557878191 29.869481509674582 26.153522961220116 4 12.755061194919385 16.921979072256754 36.437391217543166 33.88556851528069 5 13.808769097252272 37.41783143731008 33.253882500129805 15.519516965307842 6 31.655613025806222 35.70682886036312 17.331360475622233 15.306197638208424 7 28.712795419423152 33.55227056330773 19.793781101659974 17.94115291560914 8 26.917381984847438 34.51144344459042 19.092673627231154 19.478500943330996 9 27.25872938534809 16.77610415075096 19.897592605952912 36.06757385794804 10-11 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26.244103705134496 29.694231245663538 21.770095074319805 36-37 22.35724897468228 25.917611506775557 29.628662709061697 22.09647680948047 38-39 21.928037112507102 26.096651227812355 29.82688879062143 22.148422869059107 40-41 22.00397540680017 26.096298674041336 29.91631892145485 21.983406997703646 42-43 22.189100795235255 25.954448983824907 29.64033537914116 22.21611484179868 44-45 21.944830872196583 26.319478304019 29.4389891955212 22.296701628263225 46-47 22.209424456062017 26.551496647314472 28.952670321807446 22.28640857481607 48-49 22.397149785384627 26.571027492394773 28.727945326283017 22.30387739593759 50-51 22.261552064318778 26.80689774540089 28.49923594045249 22.432314249827844 52-53 22.477094679439062 26.898598490841945 28.39408135946166 22.230225470257334 54-55 22.173969937583944 26.740884490795608 28.402045310895158 22.68310026072529 56-57 22.263727675733826 27.106807687019536 28.363786893618364 22.265677743628277 58-59 22.476065284935203 26.60511226156639 28.34683560055543 22.571986852942977 60-61 22.288391479743247 26.667757829898513 28.7669139558543 22.276936734503938 62-63 22.23838350518722 26.665458479768382 28.693163160044104 22.402994855000287 64-65 22.12588826171281 26.628693766466665 28.60427372878343 22.641144243037097 66-67 22.115541757205992 26.810848228194157 28.47200724018361 22.60160277441624 68-69 21.992237746126342 26.682704476519515 28.62334202637942 22.701715750974728 70-71 22.01070216904963 26.821592081589902 28.413214435706617 22.754491313653848 72-73 22.21994800295292 26.718803623497923 28.2074598232693 22.85378855027986 74-75 22.010678568280845 27.01603217148911 28.060558794215986 22.912730466014064 76-77 22.157183040743405 26.932762074579582 28.025988027854048 22.88406685682297 78-79 22.264331736084422 26.911706518903245 27.875041868852808 22.948919876159522 80-81 22.067825620318807 27.27872327663302 27.901553694315957 22.751897408732212 82-83 22.320294026174057 27.114757092715003 27.740599406616084 22.82434947449486 84-85 22.27157952416566 26.834583515368728 27.67772207700529 23.216114883460325 86-87 22.37489426267484 26.58185136522406 27.92961195543272 23.113642416668373 88-89 22.392271575440393 26.706841093875706 27.73322000733886 23.16766732334504 90-91 22.26489766827698 26.825795820759836 27.751444279885817 23.15786223107737 92-93 22.410457722955794 26.459886418222478 27.68493467010285 23.44472118871888 94-95 22.3227398777209 26.37592390315171 27.642278430558072 23.65905778856932 96-97 22.484458828427538 26.33899533925364 27.50796011226882 23.66858572005 98-99 22.343427589573388 26.42912115601279 27.290738233689925 23.936713020723897 100-101 23.078715503883057 25.330959853380612 27.37685081151418 24.213473831222153 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2865.0 1 1861.5 2 1180.0 3 1557.0 4 1216.5 5 1047.5 6 2074.0 7 4560.5 8 7259.5 9 8209.0 10 6384.0 11 4140.5 12 3659.5 13 3782.0 14 3892.5 15 3906.5 16 3936.5 17 4010.0 18 4192.5 19 4348.0 20 4581.0 21 5057.5 22 5787.5 23 6853.5 24 8052.0 25 9461.0 26 11144.5 27 13245.0 28 15307.0 29 17455.5 30 20167.5 31 22882.0 32 25727.0 33 28883.5 34 32391.0 35 35190.5 36 38541.0 37 42534.0 38 45844.5 39 48143.0 40 51102.5 41 57008.0 42 62479.0 43 66396.0 44 70983.5 45 76072.5 46 77452.0 47 78050.5 48 81382.0 49 81569.0 50 78376.5 51 74174.0 52 70613.5 53 69645.0 54 68739.0 55 64648.0 56 58382.5 57 52788.0 58 47919.5 59 42474.0 60 36681.5 61 31054.5 62 25777.0 63 20674.0 64 16479.5 65 13404.0 66 10407.0 67 8265.5 68 6370.0 69 4310.0 70 3118.5 71 2372.0 72 1872.0 73 1503.0 74 1152.5 75 941.0 76 663.5 77 368.0 78 202.0 79 132.0 80 80.0 81 43.0 82 27.0 83 17.5 84 15.5 85 11.5 86 5.0 87 7.0 88 6.5 89 6.0 90 6.0 91 2.5 92 1.0 93 1.5 94 2.0 95 1.0 96 0.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14377112702645475 2 0.05172404432671631 3 0.1506314726003227 4 0.1284710757466055 5 0.1935703261921578 6 0.1988019566297837 7 0.16459893876870124 8 0.06894894076757889 9 0.12116653513558066 10-11 0.10130114597384086 12-13 0.15112502264160815 14-15 0.13609642388446586 16-17 0.13439367624203102 18-19 0.13375206118835992 20-21 0.1345170637523524 22-23 0.19818501907817682 24-25 0.21333700534564048 26-27 0.14717662231132445 28-29 0.12647219807939936 30-31 0.09784629568484263 32-33 0.12425122289361479 34-35 0.09271337525547384 36-37 0.08708690478481958 38-39 0.05189678684116622 40-41 0.05858438990058422 42-43 0.062409402720546535 44-45 0.040446425883343524 46-47 0.05152662431020213 48-49 0.043728533657891845 50-51 0.05421647203520788 52-53 0.028157029855335546 54-55 0.05019403919873138 56-57 0.02795960983882136 58-59 0.051575979314330674 60-61 0.038274805701687495 62-63 0.05251372439277304 64-65 0.0411127184390789 66-67 0.038200773195494676 68-69 0.06606167302605895 70-71 0.05100839676685239 72-73 0.05044081421937411 74-75 0.042716756073256645 76-77 0.02146942679591755 78-79 0.023024109425966756 80-81 0.026923154752121894 82-83 0.023048786928031025 84-85 0.021321361783531913 86-87 0.0237150794837664 88-89 0.06416150536710992 90-91 0.10880310660137987 92-93 0.07919010412425222 94-95 0.07435331371965469 96-97 0.06682667559005141 98-99 0.0546606670723648 100-101 0.13160511850876816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2026137.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.9243818868149 #Duplication Level Percentage of deduplicated Percentage of total 1 75.97535472864664 38.68997978187829 2 13.033414563039592 13.274371609948057 3 4.500985349250503 6.876296903765747 4 2.0665185762240736 4.209447246073269 5 1.1485732782173117 2.924519212246464 6 0.7046352843678618 2.1529869787244045 7 0.46887597313198076 1.6714053379327465 8 0.32090021232471494 1.3073315967987016 9 0.24643194524716105 1.129445503998938 >10 1.3318725676296785 12.491828462495258 >50 0.12281537070460397 4.293816114386509 >100 0.07476225679442855 6.9621522876410395 >500 0.003013134541314518 0.9597055517985398 >1k 0.0015551662148720082 1.226496666564341 >5k 9.719788842950051E-5 0.415481376130638 >10k+ 1.9439577685900102E-4 1.414735369617095 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15313 0.7557731782204263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13269 0.6548915497816782 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8394 0.4142859046550159 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3199 0.15788665820721898 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2559 0.12629945556494945 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.11479973960299822 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.9355004128546095E-5 0.0 4 0.0 0.0 0.0 4.9355004128546095E-5 0.0 5 0.0 0.0 0.0 4.9355004128546095E-5 0.0 6 0.0 0.0 0.0 4.9355004128546095E-5 0.0 7 0.0 0.0 0.0 4.9355004128546095E-5 0.0 8 0.0 0.0 0.0 4.9355004128546095E-5 0.0 9 0.0 0.0 0.0 9.871000825709219E-5 0.0 10-11 0.0 0.0 0.0 9.871000825709219E-5 0.0 12-13 0.0 0.0 0.0 1.480650123856383E-4 0.0 14-15 0.0 0.0 0.0 1.7274251444991133E-4 0.0 16-17 0.0 0.0 0.0 1.9742001651418438E-4 0.0 18-19 0.0 0.0 0.0 1.9742001651418438E-4 0.0 20-21 0.0 0.0 0.0 2.467750206427305E-4 0.0 22-23 0.0 0.0 0.0 3.4548502889982267E-4 0.0 24-25 0.0 0.0 0.0 9.377450784423758E-4 0.0 26-27 0.0 0.0 0.0 0.0016780701403705672 0.0 28-29 0.0 0.0 0.0 0.005132920429368794 0.0 30-31 0.0 0.0 0.0 0.014905211246820921 0.0 32-33 0.0 0.0 0.0 0.033141885272318705 0.0 34-35 0.0 0.0 0.0 0.060065040024440595 0.0 36-37 0.0 0.0 0.0 0.10181937351719059 0.0 38-39 0.0 0.0 0.0 0.16454958376457268 0.0 40-41 0.0 0.0 0.0 0.23381933205898714 0.0 42-43 0.0 0.0 0.0 0.30395279292565114 0.0 44-45 0.0 0.0 0.0 0.3845741921696312 0.0 46-47 0.0 0.0 0.0 0.47842273252006157 0.0 48-49 0.0 0.0 0.0 0.5791316184443598 0.0 50-51 0.0 0.0 0.0 0.6871203674776187 0.0 52-53 0.0 0.0 0.0 0.7924439462879361 0.0 54-55 0.0 0.0 0.0 0.9060838432939136 0.0 56-57 0.0 0.0 0.0 1.0363316991891467 0.0 58-59 0.0 0.0 0.0 1.1777831410215598 0.0 60-61 0.0 0.0 0.0 1.3282665486094967 0.0 62-63 0.0 0.0 0.0 1.4752210734022428 0.0 64-65 0.0 0.0 0.0 1.6281969086986714 0.0 66-67 0.0 0.0 0.0 1.8020252332394109 0.0 68-69 0.0 0.0 0.0 1.9968787895389106 0.0 70-71 2.4677502064273047E-5 0.0 0.0 2.202146251709534 0.0 72-73 4.9355004128546095E-5 0.0 0.0 2.4078332314152497 0.0 74-75 4.9355004128546095E-5 0.0 0.0 2.6079184181523756 0.0 76-77 4.9355004128546095E-5 0.0 0.0 2.823254301165222 0.0 78-79 4.9355004128546095E-5 0.0 0.0 3.0607752585338504 0.0 80-81 4.9355004128546095E-5 0.0 0.0 3.319889030208717 0.0 82-83 4.9355004128546095E-5 0.0 0.0 3.5800145794682194 0.0 84-85 4.9355004128546095E-5 0.0 0.0 3.842879331456856 0.0 86-87 4.9355004128546095E-5 0.0 0.0 4.107841671120956 0.0 88-89 4.9355004128546095E-5 0.0 0.0 4.392965529971567 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6450 0.0 54.98989 1 GTATCAA 11255 0.0 41.200245 1 TATCAAC 14450 0.0 31.928722 2 ATCAACG 14650 0.0 31.430235 3 TCAACGC 14790 0.0 31.164883 4 CAACGCA 15020 0.0 30.592648 5 AACGCAG 15350 0.0 29.960752 6 GTGGTAT 2715 0.0 28.582756 1 ACGCAGA 17230 0.0 26.520155 7 CGCAGAG 17305 0.0 26.23711 8 TGGTATC 2850 0.0 26.20188 2 GTACATG 11105 0.0 25.422745 1 GAGTACT 10270 0.0 24.85464 12-13 GCAGAGT 18200 0.0 24.502796 9 TACATGG 11360 0.0 24.326265 2 ACATGGG 11630 0.0 23.231567 3 GTACTTT 10995 0.0 23.020065 14-15 AGTACTT 10550 0.0 22.167477 12-13 CAGAGTA 17835 0.0 21.84243 10-11 GTACCTG 1740 0.0 21.341831 1 >>END_MODULE