FastQCFastQC Report
Thu 26 May 2016
SRR938140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026137
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156920.7744787247851453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133970.661208990310132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88110.43486694137661963No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34500.17027476424348403No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26000.12832301073421984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24360.1202287900571383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68700.053.666381
GTATCAA115250.038.503771
TATCAAC145900.030.5430722
ATCAACG147300.030.0863153
TCAACGC148500.029.838044
CAACGCA150350.029.502485
AACGCAG155350.028.5835026
GTGGTAT28950.026.258431
ACGCAGA176000.025.1212587
CGCAGAG176800.024.9001438
GAGTACT107600.023.72363712-13
TGGTATC31050.023.5626582
GCAGAGT187750.023.2455569
GTACATG113450.023.2426571
GTACTTT111850.022.69482414-15
TACATGG115400.022.4365392
ACATGGG115200.021.6057283
CAGAGTA182950.021.40267210-11
GTGTACG2005.377915E-721.3801061
AGTACTT108950.021.22837612-13
Produced by FastQC (version 0.11.5)