##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938140_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2026137 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52890944689327 34.0 31.0 34.0 31.0 34.0 2 32.73805127688799 34.0 31.0 34.0 31.0 34.0 3 32.78340408373175 34.0 31.0 34.0 31.0 34.0 4 36.24374709113945 37.0 35.0 37.0 35.0 37.0 5 36.222201164087124 37.0 37.0 37.0 35.0 37.0 6 36.25983238053498 37.0 37.0 37.0 35.0 37.0 7 36.21699569180169 37.0 37.0 37.0 35.0 37.0 8 36.20902584573501 37.0 37.0 37.0 35.0 37.0 9 37.888082592638106 39.0 38.0 39.0 35.0 39.0 10-11 37.871337673612395 39.0 38.0 39.0 35.0 39.0 12-13 37.78366122330326 39.0 38.0 39.0 35.0 39.0 14-15 39.04093232589899 40.0 38.0 41.0 35.5 41.0 16-17 38.93256601108415 40.0 38.0 41.0 35.5 41.0 18-19 39.20066683546078 40.5 39.0 41.0 36.0 41.0 20-21 39.200175259619655 41.0 39.0 41.0 36.0 41.0 22-23 39.104966248580425 40.0 39.0 41.0 35.5 41.0 24-25 39.00675645329018 40.0 38.5 41.0 35.0 41.0 26-27 38.84083504718585 40.0 38.0 41.0 35.0 41.0 28-29 38.7191895217352 40.0 38.0 41.0 35.0 41.0 30-31 38.46821685799134 40.0 38.0 41.0 34.0 41.0 32-33 38.242580092066824 40.0 38.0 41.0 34.0 41.0 34-35 38.07736076089623 40.0 37.0 41.0 34.0 41.0 36-37 38.07097027496167 40.0 37.5 41.0 34.0 41.0 38-39 37.94122386590837 40.0 37.0 41.0 33.0 41.0 40-41 37.75992146631744 40.0 37.0 41.0 33.0 41.0 42-43 37.51881215337364 40.0 37.0 41.0 33.0 41.0 44-45 37.20528917837244 40.0 36.5 41.0 32.0 41.0 46-47 36.75182773919039 40.0 36.0 41.0 31.0 41.0 48-49 36.32248362277575 39.0 35.5 41.0 30.0 41.0 50-51 35.947507251483984 39.0 35.0 41.0 29.0 41.0 52-53 35.44618971964877 38.5 35.0 40.0 27.5 41.0 54-55 35.14275589459153 38.0 35.0 40.0 27.0 41.0 56-57 34.81814901953817 38.0 34.0 40.0 26.0 41.0 58-59 34.40000848906071 38.0 34.0 40.0 25.5 41.0 60-61 33.95173475436261 37.0 33.0 40.0 24.0 41.0 62-63 33.50366683003173 36.5 33.0 39.5 23.0 41.0 64-65 33.025573048614184 36.0 32.0 39.0 22.0 40.0 66-67 32.49488904254747 35.5 31.5 39.0 20.0 40.0 68-69 31.947317234718085 35.0 31.0 38.0 18.0 40.0 70-71 31.362673402637633 35.0 30.5 37.5 14.5 39.5 72-73 30.7926228581779 34.5 30.0 37.0 9.5 39.0 74-75 30.194356304632905 34.0 29.0 36.0 7.0 39.0 76-77 28.834308834989933 32.5 28.0 35.0 6.5 37.0 78-79 29.537206023087283 34.0 29.0 35.0 2.0 37.0 80-81 29.211165138388964 34.0 29.0 35.0 2.0 37.0 82-83 28.6663431446146 34.0 28.5 35.0 2.0 36.5 84-85 28.0946049057887 33.5 27.0 35.0 2.0 36.0 86-87 27.4547315902133 33.0 26.0 35.0 2.0 35.5 88-89 26.74679550296944 32.5 25.0 35.0 2.0 35.0 90-91 26.226372895811092 32.0 24.5 34.5 2.0 35.0 92-93 25.423535032428706 32.0 21.5 34.0 2.0 35.0 94-95 24.62054022013319 31.0 18.0 34.0 2.0 35.0 96-97 23.587758132840968 31.0 4.5 34.0 2.0 35.0 98-99 22.168586823102288 30.5 2.0 34.0 2.0 35.0 100-101 17.799765514375384 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1527894417105955 1101 2 0.13981758658110977 1101 3 0.12772296848552855 1101 4 0.15708940002751604 1101 5 0.12790226500750634 1101 6 0.06745779395470919 1101 7 0.07410687281630146 1101 8 0.08120806278861181 1101 9 0.015026290734333259 1101 10-11 0.12657786647738334 1101 12-13 0.18791600700447475 1101 14-15 0.20376398396393824 1101 16-17 0.3081304115717316 1101 18-19 0.29388565481618656 1101 20-21 0.27229040929712767 1101 22-23 0.2834505421487634 1101 24-25 0.27260853203190294 1101 26-27 0.24775610971500583 1101 28-29 0.2802871689113786 1101 30-31 0.33161974339290623 1101 32-33 0.4178893166418689 1101 34-35 0.30477494202030186 1101 36-37 0.47505680370834824 1101 38-39 0.4543471027511572 1101 40-41 0.42867800143624635 1101 42-43 0.40338484314555245 1101 44-45 0.38875910678851966 1101 46-47 0.3876388273085709 1101 48-49 0.519592411649711 1101 50-51 0.3211371262887468 1101 52-53 0.10410089893374419 1101 54-55 0.12342267316844158 1101 56-57 0.3086030304038303 1101 58-59 0.5027799912466193 1101 60-61 0.6089812051806476 1101 62-63 0.3485041712776109 1101 64-65 0.2370026772486895 1101 66-67 0.1954093620474353 1101 68-69 0.22402792315159203 1101 70-71 0.308160468330108 1101 72-73 0.10552096945227163 1101 74-75 -0.029338846958964382 1101 76-77 -0.0646919035045137 1101 78-79 -0.1302591353022997 1101 80-81 -0.36991537264158936 1101 82-83 -0.3413130635963917 1101 84-85 -0.26067314094060734 1101 86-87 -0.4228283112864659 1101 88-89 -0.46544167100314837 1101 90-91 -0.45072529916842186 1101 92-93 -0.6768105244384408 1101 94-95 -0.7447602809248508 1101 96-97 -0.9196805698910246 1101 98-99 -0.9300536258392249 1101 100-101 -1.2715191032738247 1102 1 0.18150706312590614 1102 2 0.15065047085326455 1102 3 0.06914373434821641 1102 4 0.08748428085750959 1102 5 0.07619016354133379 1102 6 0.09906596801464929 1102 7 0.11898496385406077 1102 8 0.12616895943320117 1102 9 0.12876361351440835 1102 10-11 0.16463850820810677 1102 12-13 0.19368055117234917 1102 14-15 0.24508334480656657 1102 16-17 0.2535315792581727 1102 18-19 0.2934872324540905 1102 20-21 0.22341439986492873 1102 22-23 0.2308750329898217 1102 24-25 0.29928372130641634 1102 26-27 0.3038422581204898 1102 28-29 0.29160319205109175 1102 30-31 0.22422724099472902 1102 32-33 0.2784414899068679 1102 34-35 0.4885591200345232 1102 36-37 0.4410112784596407 1102 38-39 0.38897016525920236 1102 40-41 0.4000323572022566 1102 42-43 0.41700827636792326 1102 44-45 0.34539126262612996 1102 46-47 0.38946469093919944 1102 48-49 0.32011147541130214 1102 50-51 0.32962852278620147 1102 52-53 0.22860135818637417 1102 54-55 0.3711085749406138 1102 56-57 0.41109152997897525 1102 58-59 0.3059115719037351 1102 60-61 0.36990294623860365 1102 62-63 0.3829860183777001 1102 64-65 0.2617395783571439 1102 66-67 0.17168714810576802 1102 68-69 0.13259874933498494 1102 70-71 0.29858527383247946 1102 72-73 0.26915822484132335 1102 74-75 0.24743443511132668 1102 76-77 0.0782251596736181 1102 78-79 0.14036360107904855 1102 80-81 0.02341731098621125 1102 82-83 -0.03391890508607531 1102 84-85 -0.12992850196668115 1102 86-87 -0.12814385797545214 1102 88-89 -0.2556562139607905 1102 90-91 -0.3366602572751596 1102 92-93 -0.34953091793068936 1102 94-95 -0.2411926332028358 1102 96-97 -0.23067868319711948 1102 98-99 -0.29592382996141353 1102 100-101 -0.8902110676903483 1103 1 0.13561967820177045 1103 2 0.09804767300332173 1103 3 0.16920402705743243 1103 4 0.05673987622210319 1103 5 0.07996836440445065 1103 6 0.06345821567685306 1103 7 0.05357042252063593 1103 8 0.030026778373404284 1103 9 0.1164036081252533 1103 10-11 0.08519006152264552 1103 12-13 0.054062693557021646 1103 14-15 0.0344499421579485 1103 16-17 0.12159281202156791 1103 18-19 0.13618361507442955 1103 20-21 0.14414899550447302 1103 22-23 0.19743818396361945 1103 24-25 0.26578560415811125 1103 26-27 0.3055425247130259 1103 28-29 0.2659891543964932 1103 30-31 0.17970497689071152 1103 32-33 0.2058799154441573 1103 34-35 0.20315269901340116 1103 36-37 0.13468849970340102 1103 38-39 0.13273834375488747 1103 40-41 0.16931687965534792 1103 42-43 0.11199102680060946 1103 44-45 0.08782922179412367 1103 46-47 0.07703430341376105 1103 48-49 -0.059468621532545285 1103 50-51 -0.1697264854407976 1103 52-53 -0.21011893668093506 1103 54-55 -0.178418014509937 1103 56-57 -0.15570642116625066 1103 58-59 -0.030537291161103042 1103 60-61 -0.1729568485223396 1103 62-63 -0.1101111065318463 1103 64-65 -0.12169065615041319 1103 66-67 -0.30423935697155713 1103 68-69 -0.17113004076896488 1103 70-71 -0.3784358578781344 1103 72-73 -0.5781421066161663 1103 74-75 -0.564602586684881 1103 76-77 -0.29986087905880865 1103 78-79 -0.32526924985165095 1103 80-81 -0.2932698393511295 1103 82-83 -0.3285391677038021 1103 84-85 -0.3881183124378609 1103 86-87 -0.5028775245342132 1103 88-89 -0.6823298548296108 1103 90-91 -0.933388295701544 1103 92-93 -0.7611731413599827 1103 94-95 -0.7097726027333735 1103 96-97 -0.6896613519389767 1103 98-99 -0.9004902254023328 1103 100-101 -1.37115835820671 1104 1 0.2288251072993006 1104 2 0.16854958780898954 1104 3 0.1637639406036442 1104 4 0.12214959225238431 1104 5 0.1288445904095994 1104 6 0.13731004399457447 1104 7 0.10345757254050625 1104 8 0.07600802090748715 1104 9 0.20436766238889703 1104 10-11 0.12852527948481196 1104 12-13 0.12239643114609322 1104 14-15 0.24166075411982035 1104 16-17 0.2627140750758983 1104 18-19 0.2653044613467941 1104 20-21 0.20648409048178706 1104 22-23 0.13917183751304663 1104 24-25 0.046432885908409105 1104 26-27 0.15813465801037552 1104 28-29 0.11600814302836682 1104 30-31 0.19769620738822624 1104 32-33 0.15923051220532614 1104 34-35 0.256622351934233 1104 36-37 0.17420008476165805 1104 38-39 0.26694541037281283 1104 40-41 0.297367871676137 1104 42-43 0.22719081453789158 1104 44-45 0.4691286363635001 1104 46-47 0.6011094403197959 1104 48-49 0.4546460242540249 1104 50-51 0.4763578707734055 1104 52-53 0.3268612103770465 1104 54-55 0.25015056719015405 1104 56-57 0.4146994117315117 1104 58-59 0.30708684666391406 1104 60-61 0.19814606929304546 1104 62-63 0.285197334897191 1104 64-65 0.26931004190245034 1104 66-67 0.07678106420598141 1104 68-69 0.09781432225495834 1104 70-71 -0.09504177231028876 1104 72-73 -0.132743390896767 1104 74-75 -0.0987660392337979 1104 76-77 -0.07952371269789538 1104 78-79 -0.024481919112385953 1104 80-81 0.01758858345937142 1104 82-83 -0.21266125521207613 1104 84-85 -0.02725872241011018 1104 86-87 -0.16591941226043616 1104 88-89 -0.29237296215552533 1104 90-91 -0.3756852027466415 1104 92-93 -0.39722483826611565 1104 94-95 -0.5494719674255677 1104 96-97 -0.8088053375816351 1104 98-99 -0.9478519158517607 1104 100-101 -1.5501701977752624 1105 1 0.16309754218730177 1105 2 0.09923597179081867 1105 3 0.07073101160570161 1105 4 0.06382158749325129 1105 5 0.06676272442244624 1105 6 0.025096633802519364 1105 7 0.03728701999121853 1105 8 0.09121990728637996 1105 9 0.06816691988787227 1105 10-11 0.14231316405675898 1105 12-13 0.13173416095079205 1105 14-15 0.17153552467301125 1105 16-17 0.04817971293008583 1105 18-19 0.04843427495803354 1105 20-21 0.0622912819891539 1105 22-23 0.14686089055383178 1105 24-25 0.15216253473150942 1105 26-27 0.1771513054305487 1105 28-29 0.13784933242974517 1105 30-31 0.22918450202179486 1105 32-33 0.22361400412339094 1105 34-35 0.22462148027492645 1105 36-37 0.20137541553091154 1105 38-39 0.16608544212157028 1105 40-41 0.2508832195604498 1105 42-43 0.18397528245986905 1105 44-45 0.1484828767951356 1105 46-47 0.12011336278666107 1105 48-49 0.20473072472452714 1105 50-51 0.03151418091869829 1105 52-53 0.04055413498488036 1105 54-55 0.1849654032132193 1105 56-57 0.1780117011732969 1105 58-59 0.2836045359396451 1105 60-61 0.17175173253351517 1105 62-63 0.145887329584518 1105 64-65 0.18743132979496835 1105 66-67 0.05286530975940451 1105 68-69 0.1476050763038863 1105 70-71 -0.12737428806537565 1105 72-73 -0.11831965631384733 1105 74-75 -0.20662393649888955 1105 76-77 -0.1030501631039833 1105 78-79 -0.25889623722985533 1105 80-81 -0.27368241669014637 1105 82-83 -0.09189667639212473 1105 84-85 -0.308733496780782 1105 86-87 -0.2720960250687412 1105 88-89 -0.5466226989080525 1105 90-91 -0.7993788272269065 1105 92-93 -0.5809421470657377 1105 94-95 -0.5230386604965105 1105 96-97 -0.6477460011243252 1105 98-99 -0.7166204266822866 1105 100-101 -1.360845161111488 1106 1 0.13753847842488653 1106 2 0.11433250796579841 1106 3 0.14609347590931776 1106 4 0.07590108467042711 1106 5 0.11844819477544632 1106 6 0.1216384793763936 1106 7 0.09281204885454031 1106 8 0.07590108386637695 1106 9 0.06931323025984426 1106 10-11 0.21503440791267536 1106 12-13 0.04626783406605739 1106 14-15 0.15897374356243432 1106 16-17 0.1515583089366288 1106 18-19 0.22452972011092953 1106 20-21 0.22713527830553915 1106 22-23 0.2217865774327592 1106 24-25 0.25771991432780794 1106 26-27 0.18987498197085273 1106 28-29 0.18927353021096138 1106 30-31 0.18895674832513265 1106 32-33 0.3019444567954821 1106 34-35 0.10141381295274243 1106 36-37 0.18621933812958247 1106 38-39 0.17051757759884367 1106 40-41 0.18963585355088952 1106 42-43 0.11801749662228644 1106 44-45 0.060838900439236454 1106 46-47 0.12386587769464796 1106 48-49 0.2117602753186958 1106 50-51 0.08797840174519678 1106 52-53 -0.009298316185642364 1106 54-55 8.127542524150044E-4 1106 56-57 -0.22878600722666675 1106 58-59 -0.06179482691253213 1106 60-61 -0.1873983930134102 1106 62-63 -0.14917446286723646 1106 64-65 -0.21790804385400975 1106 66-67 -0.24587442930259584 1106 68-69 -0.25992357153188195 1106 70-71 -0.800875011633039 1106 72-73 -0.7601394856970423 1106 74-75 -0.5597586970070658 1106 76-77 -0.5372277626739219 1106 78-79 -0.4554975389711835 1106 80-81 -0.4573252793415783 1106 82-83 -0.644309683825135 1106 84-85 -0.4741621312339461 1106 86-87 -0.4792237903334069 1106 88-89 -0.6223242646721872 1106 90-91 -0.9888193041464604 1106 92-93 -1.0601998267498196 1106 94-95 -0.899248339745089 1106 96-97 -1.1919443040292599 1106 98-99 -1.368214373664511 1106 100-101 -1.8063901731444894 1107 1 -0.006002957717441859 1107 2 0.048163011222953855 1107 3 -0.011074548251016836 1107 4 -0.0944484248847175 1107 5 -0.031130205683794543 1107 6 -0.05567875815864909 1107 7 0.07330137055141961 1107 8 0.009858462170328153 1107 9 -0.002091422102530771 1107 10-11 0.022465645797147715 1107 12-13 0.06308605748261442 1107 14-15 0.02947487919809788 1107 16-17 -0.01599474979196458 1107 18-19 -0.01230023404766456 1107 20-21 0.04754000970482508 1107 22-23 0.05764614547522484 1107 24-25 0.1334588054814958 1107 26-27 0.06850132039860313 1107 28-29 0.09703277129019483 1107 30-31 0.05144499678391412 1107 32-33 -0.037988112237023586 1107 34-35 -0.05725331268266842 1107 36-37 -0.0994013512387042 1107 38-39 -0.043789975834272354 1107 40-41 -0.05820363079563862 1107 42-43 0.09710287312918808 1107 44-45 0.003116473922766261 1107 46-47 -0.18701438553987515 1107 48-49 -0.18583804244298108 1107 50-51 -0.35493250701292567 1107 52-53 -0.16134974009805347 1107 54-55 -0.08999910045277915 1107 56-57 -0.17043232535440467 1107 58-59 -0.30067298110938623 1107 60-61 -0.4100994587638027 1107 62-63 -0.30880147102883626 1107 64-65 -0.4392270035618253 1107 66-67 -0.4901322934755754 1107 68-69 -0.36767324498462983 1107 70-71 -0.3928557807038118 1107 72-73 -0.5833235085927875 1107 74-75 -0.7006575963140378 1107 76-77 -0.791449477858162 1107 78-79 -0.6778382587894818 1107 80-81 -0.5341309608477722 1107 82-83 -0.6258701637040005 1107 84-85 -0.7885985356367158 1107 86-87 -0.8067496437240926 1107 88-89 -0.8982327732040147 1107 90-91 -1.037019862396665 1107 92-93 -0.8649916476771331 1107 94-95 -0.8994246128633847 1107 96-97 -1.2937126509929904 1107 98-99 -1.1805266609928147 1107 100-101 -1.7815278637690461 1108 1 0.030299369097818385 1108 2 0.012630843500915034 1108 3 -0.10262642092927621 1108 4 -6.0353971129245565E-5 1108 5 0.035447079296965 1108 6 0.043323951083344525 1108 7 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-0.6318826568213609 2316 44-45 -0.6103178185353002 2316 46-47 -0.4952235667888658 2316 48-49 -0.36655345833847264 2316 50-51 -0.2066984244776151 2316 52-53 -0.10597412455958732 2316 54-55 -0.29977192283975995 2316 56-57 -0.23200780542032362 2316 58-59 -0.19593642150035606 2316 60-61 0.08149514486336784 2316 62-63 0.13622677604145395 2316 64-65 0.06986032475693804 2316 66-67 0.1224215393307091 2316 68-69 0.2666611939121104 2316 70-71 0.33654461526990787 2316 72-73 0.5382944334628554 2316 74-75 0.7985108066541251 2316 76-77 0.6993651888353618 2316 78-79 0.7473251885392251 2316 80-81 0.6996194205417616 2316 82-83 0.9551863953380817 2316 84-85 1.0671924867707112 2316 86-87 1.1455287344546825 2316 88-89 1.3823130782103519 2316 90-91 1.5976026235096406 2316 92-93 1.509737276681296 2316 94-95 1.9562695235476184 2316 96-97 2.1494989137074576 2316 98-99 2.6244093774646764 2316 100-101 3.3053489420031603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 17.0 5 46.0 6 76.0 7 220.0 8 447.0 9 918.0 10 1532.0 11 2626.0 12 3931.0 13 6189.0 14 8082.0 15 11407.0 16 15985.0 17 23265.0 18 23775.0 19 16735.0 20 15033.0 21 15941.0 22 16503.0 23 18199.0 24 20757.0 25 23917.0 26 27500.0 27 32434.0 28 38862.0 29 46844.0 30 56778.0 31 69924.0 32 87537.0 33 113658.0 34 154100.0 35 225827.0 36 311780.0 37 397018.0 38 227199.0 39 11064.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72442877669292 18.990068407109916 15.183722869021535 22.101779947175633 2 15.74278974187851 20.22855828404076 40.1429735678579 23.885678406222834 3 19.159518108446825 24.797661126996793 30.105882080009554 25.936938684546824 4 12.982290930968638 17.235951961787382 36.19394937262386 33.58780773462012 5 13.791750427048388 37.52611522434292 33.33687047687769 15.345263871731001 6 31.81091309678222 35.81201668611274 17.129670759226027 15.247399457879016 7 28.69835554061744 33.490035471441466 19.753698787396903 18.05791020054419 8 26.967919740866485 34.70441534802434 18.982033297847085 19.345631613262086 9 27.052267442922172 16.743537085596877 19.902059929807315 36.302135541673636 10-11 25.547852884577892 26.828738629223984 26.05418587193265 21.56922261426547 12-13 26.418530595061153 24.325810989924335 27.24054528866634 22.015113126348172 14-15 23.105742602795367 25.339377347138914 26.159213320718194 25.39566672934752 16-17 21.984582570264255 28.95611163113465 26.628344784407947 22.430961014193144 18-19 21.828953323491945 26.55896417665735 29.915770749954223 21.696311749896477 20-21 22.7394297621533 25.620528128157176 30.142112798887737 21.497929310801787 22-23 22.549240253743946 25.747790006302633 30.078864361097 21.624105378856413 24-25 22.241147717493963 25.972527650156817 30.19720163162886 21.58912300072036 26-27 21.988748400906033 26.360818785475942 29.97820814100806 21.67222467260996 28-29 21.596097330796972 26.3605887073926 30.371056634804177 21.672257327006246 30-31 21.84984587167827 25.88035516022016 30.369732756223065 21.900066211878507 32-33 21.62297021376146 26.175451117076488 30.184261972413513 22.017316696748544 34-35 22.263330092911026 26.175282414033173 29.8209179255078 21.740469567547997 36-37 22.3029646456109 25.89493997786418 29.80419544179722 21.9978999347277 38-39 21.899880113973165 26.041303179373482 30.014495893817205 22.044320812836148 40-41 22.010907655100933 25.976235131533485 30.082202260500466 21.930654952865112 42-43 22.198243259217364 25.94355162909291 29.741844047240946 22.11636106444879 44-45 21.955425521571346 26.217773033116714 29.562512307904154 22.264289137407786 46-47 22.157896152552485 26.562611434427758 29.075992202890678 22.203500210129086 48-49 22.368190511444258 26.535875131100006 28.83800357825899 22.25793077919674 50-51 22.225707356028877 26.673415377386522 28.703112336913012 22.39776492967159 52-53 22.48925653202606 26.797255772894207 28.459794022552337 22.253693672527397 54-55 22.169118327548144 26.70017263016055 28.475980468976548 22.65472857331476 56-57 22.22337949793924 27.116187657358303 28.413309998047716 22.247122846654744 58-59 22.46592018703787 26.65509363247808 28.34143593824512 22.537550242238925 60-61 22.286215606263827 26.684023686162057 28.803883365764637 22.225877341809483 62-63 22.22313546355513 26.747086204798563 28.66296779244641 22.366810539199896 64-65 22.067553508554333 26.62826071127533 28.616410647532796 22.687775132637533 66-67 22.01493228002949 26.972902386346277 28.402997315486928 22.609168018137307 68-69 21.913508629882696 26.798328330502784 28.588732769856627 22.69943026975789 70-71 22.0203109180687 26.927417073788373 28.365288499842283 22.686983508300642 72-73 22.196353535176545 26.93887029628421 28.088400822390625 22.776375346148615 74-75 22.01486468242186 27.200103069959475 27.879782319009916 22.90524992860875 76-77 22.056561450067775 27.142663914073275 28.064753081117583 22.736021554741363 78-79 22.129090702645595 27.146616114492776 27.924028573826416 22.800264609035214 80-81 21.915874368586373 27.51229632306067 27.985416927351924 22.586412381001036 82-83 22.18119412019407 27.33325730050888 27.86793098391906 22.617617595377997 84-85 22.103918343913545 27.114987272398324 27.752169542070444 23.02892484161769 86-87 22.178312896118886 26.913682339654777 27.974771817009277 22.933232947217064 88-89 22.17505080937094 27.10557143202701 27.731549793183525 22.98782796541853 90-91 22.018454380811118 27.24246447762491 27.871120974906315 22.867960166657657 92-93 22.149568512494895 26.990615930456485 27.68683105367269 23.172984503375925 94-95 21.976951477066613 26.88603561012342 27.773808263319737 23.36320464949023 96-97 22.15928821747583 26.951119373238853 27.520190972303638 23.369401436981683 98-99 22.028693708446234 27.00345942536074 27.45589707759138 23.51194978860164 100-101 22.397589852978957 26.562729446583788 27.37931557017268 23.660365130264577 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2781.0 1 1809.5 2 1176.5 3 1515.0 4 1161.0 5 985.0 6 2159.0 7 4980.5 8 7782.5 9 8537.0 10 6455.0 11 4034.5 12 3539.0 13 3747.0 14 3896.5 15 3888.0 16 3961.5 17 4073.5 18 4137.5 19 4231.5 20 4424.0 21 4920.0 22 5739.0 23 6697.0 24 7767.0 25 9348.5 26 11319.5 27 13340.5 28 15109.5 29 17207.0 30 20009.5 31 22808.0 32 25783.0 33 28926.5 34 32309.5 35 35173.0 36 38655.5 37 42594.5 38 45962.5 39 48523.0 40 51162.0 41 57274.5 42 63192.0 43 67465.5 44 72559.0 45 77824.0 46 78977.5 47 79500.0 48 83139.5 49 82552.0 50 78920.5 51 75062.5 52 71078.0 53 69696.5 54 69376.5 55 65184.5 56 58277.5 57 52410.0 58 47354.0 59 41815.5 60 35768.5 61 29858.5 62 24621.0 63 19570.5 64 15572.0 65 12342.5 66 9067.0 67 7411.0 68 5591.0 69 3418.0 70 2429.0 71 1879.5 72 1509.0 73 1251.5 74 976.0 75 827.5 76 561.0 77 274.5 78 132.5 79 72.0 80 42.0 81 18.5 82 13.5 83 13.0 84 8.5 85 6.5 86 2.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008785190734881205 2 0.015201341271592199 3 0.010068420842223404 4 0.0 5 9.87100082570922E-4 6 0.001036455086699468 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.637125722495568E-4 14-15 0.0 16-17 0.002640492720877216 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003134042762162677 26-27 0.01877957907091179 28-29 0.0068603455738679075 30-31 0.0032327527704197694 32-33 0.0 34-35 0.008217608187402924 36-37 4.688725392211879E-4 38-39 0.00227033018991312 40-41 0.0018261351527562055 42-43 0.002739202729134308 44-45 0.0 46-47 7.403250619281915E-5 48-49 5.922600495425532E-4 50-51 0.0035042052931267727 52-53 0.006835668071803634 54-55 0.0063914730346467195 56-57 0.015127308765399378 58-59 0.02225910686197429 60-61 0.002936622745648493 62-63 0.01902635409155452 64-65 0.020210874190639626 66-67 0.01270891356310062 68-69 0.03045203754731294 70-71 0.01628715136242021 72-73 0.004565337881890514 74-75 0.016213118856227393 76-77 0.020284906696832444 78-79 0.02502298709317287 80-81 0.01117890843511569 82-83 0.03435108287346808 84-85 0.020803134240182177 86-87 0.030822200078277035 88-89 0.021123941767017727 90-91 0.02702186476037899 92-93 0.023196851940416662 94-95 0.01384407865805718 96-97 0.01448569371172828 98-99 0.010981488418601505 100-101 0.009994388336030585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2026137.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.44388547378196 #Duplication Level Percentage of deduplicated Percentage of total 1 73.84793652573588 35.77480979527869 2 14.140122204588964 13.700049213287782 3 4.8477409109576275 7.0453021649099625 4 2.304950510204891 4.466430341564041 5 1.2105853887146851 2.9322729963564007 6 0.8035059899166537 2.3354971291812108 7 0.5144996098794433 1.7447052124113676 8 0.36629880893590133 1.4195950039418834 9 0.2649533399154081 1.1551832329282248 >10 1.4759170304127716 13.17688754974833 >50 0.13256715260517188 4.462445671064869 >100 0.08562908727534646 7.578430034977266 >500 0.0033592990056363656 1.0583854986718677 >1k 0.0016287510330358133 1.2809906678548333 >5k 1.0179693956473833E-4 0.4345091151243046 >10k+ 2.0359387912947667E-4 1.4345063726990008 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15692 0.7744787247851453 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13397 0.661208990310132 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8811 0.43486694137661963 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3450 0.17027476424348403 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2600 0.12832301073421984 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2436 0.1202287900571383 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.9355004128546095E-5 0.0 10-11 0.0 0.0 0.0 4.9355004128546095E-5 0.0 12-13 0.0 0.0 0.0 9.871000825709219E-5 0.0 14-15 0.0 0.0 0.0 1.2338751032136525E-4 0.0 16-17 0.0 0.0 0.0 1.480650123856383E-4 0.0 18-19 0.0 0.0 0.0 1.480650123856383E-4 0.0 20-21 0.0 0.0 0.0 1.9742001651418438E-4 0.0 22-23 0.0 0.0 0.0 2.961300247712766E-4 0.0 24-25 0.0 0.0 0.0 8.883900743138297E-4 0.0 26-27 0.0 0.0 0.0 0.0016533926383062942 0.0 28-29 0.0 0.0 0.0 0.005157597931433067 0.0 30-31 0.0 0.0 0.0 0.01497924375301374 0.0 32-33 0.0 0.0 0.0 0.03333930528883289 0.0 34-35 0.0 0.0 0.0 0.06048455755953325 0.0 36-37 0.0 0.0 0.0 0.10209082603989761 0.0 38-39 0.0 0.0 0.0 0.16541329633682222 0.0 40-41 0.0 0.0 0.0 0.2351272396683936 0.0 42-43 0.0 0.0 0.0 0.30592699309079296 0.0 44-45 0.0 0.0 0.0 0.38681984485748 0.0 46-47 0.0 0.0 0.0 0.48192693781318835 0.0 48-49 0.0 0.0 0.0 0.5830553412725793 0.0 50-51 0.0 0.0 0.0 0.6918090928698306 0.0 52-53 0.0 0.0 0.0 0.7974781567090479 0.0 54-55 0.0 0.0 0.0 0.911463538743925 0.0 56-57 0.0 0.0 0.0 1.0433401097754003 0.0 58-59 0.0 0.0 0.0 1.1865683317564408 0.0 60-61 0.0 0.0 0.0 1.3386064219744274 7.403250619281915E-5 62-63 0.0 0.0 0.0 1.4868688543765796 9.871000825709219E-5 64-65 0.0 0.0 0.0 1.6409798547679648 9.871000825709219E-5 66-67 0.0 0.0 0.0 1.8164862494490748 9.871000825709219E-5 68-69 0.0 0.0 0.0 2.012598358353853 9.871000825709219E-5 70-71 0.0 0.0 0.0 2.2198646981916816 9.871000825709219E-5 72-73 0.0 0.0 0.0 2.428093460610018 9.871000825709219E-5 74-75 0.0 0.0 0.0 2.630769785063893 9.871000825709219E-5 76-77 0.0 0.0 0.0 2.849683905876059 9.871000825709219E-5 78-79 0.0 0.0 0.0 3.092313106171991 9.871000825709219E-5 80-81 0.0 0.0 0.0 3.3578677058856337 9.871000825709219E-5 82-83 0.0 0.0 0.0 3.6233729505951473 9.871000825709219E-5 84-85 0.0 0.0 0.0 3.8942085357505443 9.871000825709219E-5 86-87 0.0 0.0 0.0 4.165340250930712 9.871000825709219E-5 88-89 0.0 0.0 0.0 4.461149468175153 9.871000825709219E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6870 0.0 53.66638 1 GTATCAA 11525 0.0 38.50377 1 TATCAAC 14590 0.0 30.543072 2 ATCAACG 14730 0.0 30.086315 3 TCAACGC 14850 0.0 29.83804 4 CAACGCA 15035 0.0 29.50248 5 AACGCAG 15535 0.0 28.583502 6 GTGGTAT 2895 0.0 26.25843 1 ACGCAGA 17600 0.0 25.121258 7 CGCAGAG 17680 0.0 24.900143 8 GAGTACT 10760 0.0 23.723637 12-13 TGGTATC 3105 0.0 23.562658 2 GCAGAGT 18775 0.0 23.245556 9 GTACATG 11345 0.0 23.242657 1 GTACTTT 11185 0.0 22.694824 14-15 TACATGG 11540 0.0 22.436539 2 ACATGGG 11520 0.0 21.605728 3 CAGAGTA 18295 0.0 21.402672 10-11 GTGTACG 200 5.377915E-7 21.380106 1 AGTACTT 10895 0.0 21.228376 12-13 >>END_MODULE