FastQCFastQC Report
Thu 26 May 2016
SRR938139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733369
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112730.4124214476713535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91140.3334346734743827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57400.2099972597918539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55650.057.3298381
GTATCAA98000.041.7523541
TATCAAC122100.033.2414742
ATCAACG121950.033.08753
TCAACGC123800.032.516284
CAACGCA125950.031.8857485
AACGCAG131400.030.6678186
TGGTATC25250.028.9866072
ACGCAGA147300.027.1886717
CGCAGAG147100.026.9995638
GTGGTAT29150.025.8486751
TACATGG97700.023.836342
GCAGAGT163350.023.6725239
GTACATG103100.023.5439241
GAGTACT100500.022.89956912-13
CAGAGTA153500.022.32787310-11
ACATGGG99200.021.9427913
GTACTTT105750.021.76390514-15
AGAGTAC145750.020.71220810-11
AGTACTT104900.020.01457412-13
Produced by FastQC (version 0.11.5)