FastQCFastQC Report
Thu 26 May 2016
SRR938139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733369
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109360.4000923402584869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90710.3318615232703671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58880.21541182328474492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50350.056.3921241
GTATCAA93050.043.4456371
ATCAACG119800.033.184663
TCAACGC120300.033.0850524
TATCAAC121050.032.9640662
CAACGCA122900.032.3833475
AACGCAG128650.031.011086
ACGCAGA147000.026.91387
CGCAGAG146600.026.8252478
TGGTATC21600.024.2204692
GCAGAGT163050.023.711069
GAGTACT100200.022.49136712-13
TACATGG93300.022.4292432
TAAGGTG15600.022.250845
GTACATG97350.022.082391
AGAGTAC146150.021.65951510-11
GTACTTT103550.021.55733714-15
ACATGGG94350.021.2695583
GGACCGA8500.021.2330346
CAGAGTA155500.020.90693710-11
Produced by FastQC (version 0.11.5)