FastQCFastQC Report
Thu 26 May 2016
SRR938138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713662
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113660.4188436142747328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91910.3386936177018361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57350.21133803694048853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52050.058.129371
GTATCAA91000.042.5310971
ATCAACG116500.032.9663163
TATCAAC117100.032.9185682
TCAACGC117250.032.7959864
CAACGCA119700.032.124725
AACGCAG123650.031.0916146
TGGTATC21600.028.6066232
ACGCAGA142350.026.7641587
CGCAGAG143800.026.4612488
GTGGTAT26200.026.0472471
GCAGAGT156750.023.5460539
TAAGGTG15350.023.5336995
GAGTACT96200.023.1344512-13
GTACATG91700.022.6384661
GTAAGGT15150.022.5894074
TACATGG88900.022.2417282
CAGAGTA147650.021.892710-11
GTACTTT102150.021.67126514-15
ACATGGG90000.021.1780933
Produced by FastQC (version 0.11.5)