##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938138_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2713662 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82091505869191 33.0 31.0 34.0 30.0 34.0 2 32.022117345491075 34.0 31.0 34.0 30.0 34.0 3 32.10211588620838 34.0 31.0 34.0 30.0 34.0 4 35.62691116284932 37.0 35.0 37.0 33.0 37.0 5 35.630919399689425 37.0 35.0 37.0 33.0 37.0 6 35.648879632024915 37.0 35.0 37.0 33.0 37.0 7 35.58537024876348 37.0 35.0 37.0 33.0 37.0 8 35.562781584441986 37.0 35.0 37.0 33.0 37.0 9 37.19338296368523 39.0 37.0 39.0 34.0 39.0 10-11 37.10633048625805 39.0 37.0 39.0 33.5 39.0 12-13 37.00463653911209 39.0 37.0 39.0 33.0 39.0 14-15 38.186537048460714 40.0 38.0 41.0 33.0 41.0 16-17 38.0338166654506 40.0 38.0 41.0 33.0 41.0 18-19 38.17181948230841 40.0 38.0 41.0 33.5 41.0 20-21 38.14732343232134 40.0 38.0 41.0 33.0 41.0 22-23 38.060865538891726 40.0 38.0 41.0 33.0 41.0 24-25 37.95610617681937 40.0 38.0 41.0 33.0 41.0 26-27 37.78198814001154 40.0 38.0 41.0 32.5 41.0 28-29 37.573056814002626 40.0 37.5 41.0 32.0 41.0 30-31 37.35807222859737 40.0 37.0 41.0 31.5 41.0 32-33 37.10864156995234 40.0 36.5 41.0 31.0 41.0 34-35 36.879914484559976 39.5 36.0 41.0 30.5 41.0 36-37 36.62571609876248 39.0 36.0 41.0 30.0 41.0 38-39 36.333809626991126 39.0 35.0 40.5 30.0 41.0 40-41 36.12358466161224 39.0 35.0 40.0 30.0 41.0 42-43 35.95959224103812 39.0 35.0 40.0 28.5 41.0 44-45 35.69423715997055 39.0 35.0 40.0 27.5 41.0 46-47 35.31544219582247 38.5 35.0 40.0 26.5 41.0 48-49 34.92421311128652 38.0 34.5 40.0 24.5 41.0 50-51 34.22910719905427 37.5 33.5 39.5 24.0 40.5 52-53 34.34970254217364 38.0 34.0 40.0 24.0 40.5 54-55 34.454684297454875 38.0 34.0 40.0 23.5 41.0 56-57 34.14477355691314 38.0 34.0 40.0 23.0 41.0 58-59 33.81772195652959 38.0 33.5 40.0 22.0 41.0 60-61 33.39018621331617 37.0 33.0 40.0 20.5 41.0 62-63 32.8439407708108 36.5 32.0 39.5 17.5 41.0 64-65 32.31549175984334 36.0 31.5 39.0 13.5 40.5 66-67 31.771926275269358 35.0 31.0 39.0 8.0 40.0 68-69 31.15689702696946 35.0 30.5 38.0 6.5 40.0 70-71 30.598688967159507 35.0 29.5 37.5 2.0 40.0 72-73 29.957064107468064 34.0 29.0 37.0 2.0 39.0 74-75 29.262601974748513 34.0 28.5 36.0 2.0 39.0 76-77 28.584571881096466 34.0 27.5 36.0 2.0 38.5 78-79 27.853160968462543 33.5 26.0 35.0 2.0 37.0 80-81 27.18478609347811 33.0 25.5 35.0 2.0 37.0 82-83 26.44526823900692 32.0 23.5 35.0 2.0 36.0 84-85 25.774788643537775 32.0 21.0 35.0 2.0 36.0 86-87 25.073573274785144 31.5 19.0 34.0 2.0 35.0 88-89 24.243253028564354 31.0 13.5 34.0 2.0 35.0 90-91 23.392397984715856 30.5 4.5 34.0 2.0 35.0 92-93 22.47480047257175 30.0 2.0 34.0 2.0 35.0 94-95 21.502252675535864 29.0 2.0 34.0 2.0 35.0 96-97 20.278662928544527 28.0 2.0 34.0 2.0 35.0 98-99 18.764990813152117 26.0 2.0 33.5 2.0 35.0 100-101 15.148304210325383 10.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34255210636138855 1101 2 0.29276697119009043 1101 3 0.27485412254951314 1101 4 0.2555529482390142 1101 5 0.2298908767128296 1101 6 0.23627665962617783 1101 7 0.3264612241148299 1101 8 0.27732675883278546 1101 9 0.3436497252030435 1101 10-11 0.357805806499492 1101 12-13 0.4308984052951743 1101 14-15 0.567510560140434 1101 16-17 0.6109045604432097 1101 18-19 0.5120876713682421 1101 20-21 0.5205935875519856 1101 22-23 0.5007632123032622 1101 24-25 0.5675997565484678 1101 26-27 0.6507511755466808 1101 28-29 0.7003710905009655 1101 30-31 0.6955208999570175 1101 32-33 0.6982119987854176 1101 34-35 0.6664053068093452 1101 36-37 0.7396386347780535 1101 38-39 0.7516214765477898 1101 40-41 0.8511713618689001 1101 42-43 1.0314339198219287 1101 44-45 0.9755834986708365 1101 46-47 1.1074961791810267 1101 48-49 1.2364085255385504 1101 50-51 1.2338597084591356 1101 52-53 1.1574788822160684 1101 54-55 1.1978091750566122 1101 56-57 1.2544506185769748 1101 58-59 1.2133880793122458 1101 60-61 1.148769452571571 1101 62-63 1.198752195320992 1101 64-65 1.1342204199737438 1101 66-67 1.041089737949303 1101 68-69 1.4338538271108625 1101 70-71 1.6757054765831292 1101 72-73 1.9970771621644232 1101 74-75 2.0499471847876336 1101 76-77 2.084674981599182 1101 78-79 2.311765633212385 1101 80-81 2.6643063079794125 1101 82-83 2.7228249398345596 1101 84-85 2.7559521123438664 1101 86-87 2.7453618418513734 1101 88-89 3.2675437987286706 1101 90-91 3.5371413643045138 1101 92-93 3.889743262378545 1101 94-95 3.9958551174393477 1101 96-97 4.319711635438697 1101 98-99 4.392288133905758 1101 100-101 4.464164199814041 1102 1 0.36940190099631565 1102 2 0.31608143987083537 1102 3 0.3089840698900801 1102 4 0.21993701429818913 1102 5 0.21584953288018482 1102 6 0.26381355939122386 1102 7 0.2377007502725661 1102 8 0.3064428986017589 1102 9 0.3682512185988216 1102 10-11 0.44453011462790215 1102 12-13 0.48963543484852323 1102 14-15 0.6745375234678903 1102 16-17 0.6869108748483441 1102 18-19 0.5425511152356037 1102 20-21 0.5552421771102232 1102 22-23 0.6005179271091308 1102 24-25 0.5786098058801556 1102 26-27 0.51704096433091 1102 28-29 0.6435380112192846 1102 30-31 0.6290083888312807 1102 32-33 0.632797610360079 1102 34-35 0.5886972774677304 1102 36-37 0.7871580303682535 1102 38-39 0.6787247799218719 1102 40-41 0.8591683202750104 1102 42-43 1.0568177677575576 1102 44-45 1.2238642987211676 1102 46-47 1.2017960489136854 1102 48-49 1.285437779961626 1102 50-51 1.2204637133095915 1102 52-53 1.3403492858532076 1102 54-55 1.3921253162307679 1102 56-57 1.3647313113220179 1102 58-59 1.3287175496552663 1102 60-61 1.438168137379222 1102 62-63 1.5087376867520632 1102 64-65 1.5668727799076123 1102 66-67 1.645341454434682 1102 68-69 1.7448535291470755 1102 70-71 1.969592779844799 1102 72-73 2.051230164173525 1102 74-75 2.0446894652740717 1102 76-77 2.2973900672214533 1102 78-79 2.4377507420490083 1102 80-81 2.4794093992978077 1102 82-83 2.7676741583422952 1102 84-85 2.777566941934449 1102 86-87 2.895992196536863 1102 88-89 3.2072181080227438 1102 90-91 3.4967667123680535 1102 92-93 3.766413400498969 1102 94-95 3.909149092726558 1102 96-97 4.1934829340210875 1102 98-99 4.262093607944422 1102 100-101 3.9740216753996958 1103 1 0.53133840683752 1103 2 0.440893420320279 1103 3 0.37858729604889874 1103 4 0.25604506653337467 1103 5 0.3941675255164938 1103 6 0.34523060827264374 1103 7 0.32501226272363937 1103 8 0.40170497961777585 1103 9 0.515001032653899 1103 10-11 0.508304396988585 1103 12-13 0.6364709360274929 1103 14-15 0.7901526122756479 1103 16-17 0.8194941370652202 1103 18-19 0.7428166874229518 1103 20-21 0.7286636523877519 1103 22-23 0.9052924561097839 1103 24-25 0.8512144165421844 1103 26-27 0.8639072665571135 1103 28-29 1.0855331355447362 1103 30-31 1.0414992556260145 1103 32-33 1.0950939249260045 1103 34-35 1.1452505862451403 1103 36-37 1.2138076329459722 1103 38-39 1.3216769560853336 1103 40-41 1.270352651419465 1103 42-43 1.3411815852501618 1103 44-45 1.3530719900282904 1103 46-47 1.3964070428804618 1103 48-49 1.3165418199551269 1103 50-51 1.2112866030422325 1103 52-53 1.3095966788610625 1103 54-55 1.3070904952222904 1103 56-57 1.3724760570995116 1103 58-59 1.4668755891338705 1103 60-61 1.492381261327182 1103 62-63 1.6314698245570298 1103 64-65 1.740864730721654 1103 66-67 1.8283554488403162 1103 68-69 1.942729704305446 1103 70-71 2.130657747281756 1103 72-73 2.2592840011618733 1103 74-75 2.387040499946089 1103 76-77 2.655451565873811 1103 78-79 2.85028261901002 1103 80-81 2.7664732296434806 1103 82-83 2.8577383696482883 1103 84-85 2.833784642809963 1103 86-87 3.065944983703556 1103 88-89 3.479099878318724 1103 90-91 3.575283425795959 1103 92-93 3.8265815865681745 1103 94-95 4.243599832472761 1103 96-97 4.274052954103524 1103 98-99 4.535660365354527 1103 100-101 4.48448795230574 1104 1 0.1883955046346628 1104 2 0.1126465537686272 1104 3 0.19337097810842607 1104 4 0.13371763490336974 1104 5 0.22233129756086356 1104 6 0.17518265265635335 1104 7 0.06579524325488961 1104 8 0.15074136001746297 1104 9 0.1785136186602898 1104 10-11 0.21110997458466585 1104 12-13 0.23610000071893467 1104 14-15 0.38863611520449126 1104 16-17 0.39005601123461275 1104 18-19 0.3407951078418918 1104 20-21 0.3083807383499533 1104 22-23 0.21243349631734532 1104 24-25 0.2860755523218117 1104 26-27 0.37946673535919473 1104 28-29 0.40228073977211665 1104 30-31 0.3582525720429359 1104 32-33 0.3276735765002954 1104 34-35 0.3373660688671478 1104 36-37 0.41579663519603827 1104 38-39 0.49886068598444666 1104 40-41 0.6372361432809299 1104 42-43 0.6415639449378077 1104 44-45 0.6509589261615432 1104 46-47 0.6518468770127583 1104 48-49 0.8687259738058088 1104 50-51 0.8552606447108673 1104 52-53 0.8077807381036095 1104 54-55 1.0075485414214143 1104 56-57 1.0970372226003917 1104 58-59 1.0189423753400106 1104 60-61 1.0333340009914735 1104 62-63 1.1860323020764412 1104 64-65 1.2260010780085082 1104 66-67 1.3190067366266724 1104 68-69 1.4780337132807269 1104 70-71 1.60202022051703 1104 72-73 1.6450831409418285 1104 74-75 1.8321181544853644 1104 76-77 1.8186656899764841 1104 78-79 2.058206318438174 1104 80-81 2.1954106463593064 1104 82-83 2.3915959451272855 1104 84-85 2.5919146504589428 1104 86-87 2.7820776350252565 1104 88-89 3.133290706505882 1104 90-91 3.160065795836953 1104 92-93 3.4407249678809215 1104 94-95 3.8224990042945315 1104 96-97 3.8727580721361043 1104 98-99 3.6890845106012264 1104 100-101 3.749373962885789 1105 1 0.20234061331760245 1105 2 0.0926968925358338 1105 3 0.08208754009763908 1105 4 -0.03555031374173012 1105 5 -0.054218391080830486 1105 6 0.1150836463957603 1105 7 0.09377437238752862 1105 8 0.10252444204692779 1105 9 0.13248540087325011 1105 10-11 0.19438252926038757 1105 12-13 0.11135679194006798 1105 14-15 0.27140093689628486 1105 16-17 0.3745706566070126 1105 18-19 0.254033936212096 1105 20-21 0.2779237786644231 1105 22-23 0.28282119841612996 1105 24-25 0.20834059629303425 1105 26-27 0.27037233178668174 1105 28-29 0.34881501926926717 1105 30-31 0.21398286856033621 1105 32-33 0.4055181680911133 1105 34-35 0.3428892945593063 1105 36-37 0.3609390778629873 1105 38-39 0.33915643459813083 1105 40-41 0.5064342326807107 1105 42-43 0.3318962944479651 1105 44-45 0.45062032608733915 1105 46-47 0.5495114389886027 1105 48-49 0.6189210114596051 1105 50-51 0.6972941838337121 1105 52-53 0.5468320562372639 1105 54-55 0.7083357113866171 1105 56-57 0.823839106460234 1105 58-59 0.8467567168748431 1105 60-61 0.9072564472015188 1105 62-63 0.9792479331661426 1105 64-65 1.1202943464518427 1105 66-67 1.3211974423387538 1105 68-69 1.3666680485984237 1105 70-71 1.4360379961852878 1105 72-73 1.5870979617979906 1105 74-75 1.7731158380998622 1105 76-77 1.7483842808921075 1105 78-79 1.8140215156651962 1105 80-81 2.0503320463519543 1105 82-83 2.154745694603342 1105 84-85 2.2749251494010245 1105 86-87 2.5504928997717435 1105 88-89 2.730450878045424 1105 90-91 2.835171503905528 1105 92-93 3.014222242033334 1105 94-95 3.253677954266621 1105 96-97 3.3799081157563506 1105 98-99 3.535013788978617 1105 100-101 3.558083973481663 1106 1 0.19521241470873463 1106 2 0.13823032047940842 1106 3 0.11128111317367484 1106 4 0.18763831953807397 1106 5 0.21319828228811843 1106 6 0.008250434568275011 1106 7 0.10746232981811232 1106 8 0.20026586031846705 1106 9 0.08483089603334548 1106 10-11 0.08180064253841834 1106 12-13 0.3526653839165306 1106 14-15 0.4024399934574632 1106 16-17 0.2381202660154642 1106 18-19 0.3173000391954304 1106 20-21 0.28104202503676134 1106 22-23 0.19351985268487937 1106 24-25 0.19280209623725142 1106 26-27 0.20607409937237975 1106 28-29 0.2512906899944909 1106 30-31 0.2434320579304341 1106 32-33 0.4100261861794863 1106 34-35 0.3829635860851184 1106 36-37 0.35758862993451856 1106 38-39 0.21627264693672288 1106 40-41 0.468678129930872 1106 42-43 0.4329904307749217 1106 44-45 0.4864909262570194 1106 46-47 0.5946166561294888 1106 48-49 0.6878825386453542 1106 50-51 0.5605391422060748 1106 52-53 0.5864965574190251 1106 54-55 0.32424002618447645 1106 56-57 0.5660167678922008 1106 58-59 0.6529557200945248 1106 60-61 0.6889343823459413 1106 62-63 0.6256336964866023 1106 64-65 0.6781880318667319 1106 66-67 0.8813797400185557 1106 68-69 0.6861646122748333 1106 70-71 0.9437401477467446 1106 72-73 1.3333823477351388 1106 74-75 1.40526355980046 1106 76-77 1.5738263070387823 1106 78-79 1.7770807127926425 1106 80-81 1.8665798637870488 1106 82-83 2.071773203353061 1106 84-85 2.1516169128615807 1106 86-87 2.000388019906861 1106 88-89 2.1321206537049946 1106 90-91 2.2817443256862937 1106 92-93 2.4651825057312813 1106 94-95 2.585512593031048 1106 96-97 2.8510603154444993 1106 98-99 3.0846520604650465 1106 100-101 3.336448950222591 1107 1 0.2061044603010629 1107 2 0.12312604220208101 1107 3 0.0579935549546704 1107 4 -0.01592603724056829 1107 5 0.10917723015665359 1107 6 0.10680443057837152 1107 7 0.13265901615382347 1107 8 0.07793443252191423 1107 9 0.16084087919183787 1107 10-11 0.14902667605415587 1107 12-13 0.13814953147049636 1107 14-15 0.29398208510041 1107 16-17 0.3706671468228251 1107 18-19 0.21407577891273633 1107 20-21 0.30567625551152133 1107 22-23 0.19076894119954346 1107 24-25 0.23346081296686094 1107 26-27 0.30046058944489573 1107 28-29 0.40242707106489206 1107 30-31 0.5051324637003987 1107 32-33 0.41175209423762027 1107 34-35 0.48030451599564117 1107 36-37 0.4616679567493307 1107 38-39 0.47892876988615996 1107 40-41 0.43108774230896074 1107 42-43 0.594864942987364 1107 44-45 0.5359041459741007 1107 46-47 0.6790530842650497 1107 48-49 0.8161625619849247 1107 50-51 0.6423263611699142 1107 52-53 0.6005513346315752 1107 54-55 0.7299312356897545 1107 56-57 0.8392287744070828 1107 58-59 0.7157969857959046 1107 60-61 0.8788826923306345 1107 62-63 0.9172257979533356 1107 64-65 1.118871332841735 1107 66-67 1.0982588721390165 1107 68-69 1.1011471075992496 1107 70-71 1.4188275968893969 1107 72-73 1.5571457490564278 1107 74-75 1.6635402969922737 1107 76-77 1.7200992265583501 1107 78-79 1.7896759628244396 1107 80-81 2.012832677922237 1107 82-83 2.163581440795049 1107 84-85 2.2505018744299825 1107 86-87 2.426165436351358 1107 88-89 2.693970754887662 1107 90-91 2.863937549689229 1107 92-93 3.030564411528708 1107 94-95 3.425187865709553 1107 96-97 3.4866269631234346 1107 98-99 3.841130757665834 1107 100-101 3.7548823955851898 1108 1 0.3262413575624805 1108 2 0.2737924784321848 1108 3 0.18357392572615083 1108 4 0.24991877837358345 1108 5 0.2685884505755993 1108 6 0.2992546110673402 1108 7 0.27648779045561156 1108 8 0.2158771581756156 1108 9 0.3273320032524083 1108 10-11 0.2840933716198393 1108 12-13 0.312568464195067 1108 14-15 0.4135294921106407 1108 16-17 0.5437219352402707 1108 18-19 0.37348882913019565 1108 20-21 0.24272463467600147 1108 22-23 0.2801409637927108 1108 24-25 0.395713002173224 1108 26-27 0.47022256689280795 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3.7072584491838967 1108 96-97 3.99881649058446 1108 98-99 4.048985244532481 1108 100-101 4.3425125090161725 1109 1 0.2448333755162011 1109 2 0.12090122344297782 1109 3 0.05200968278874285 1109 4 0.1495912875407015 1109 5 0.16600760341874832 1109 6 0.17835244581130638 1109 7 0.1086726205226114 1109 8 0.14527260278498488 1109 9 0.14497034413311383 1109 10-11 0.13268536672004672 1109 12-13 0.1135883593202962 1109 14-15 0.13479622802344693 1109 16-17 0.13692895721049325 1109 18-19 0.1690118831821863 1109 20-21 0.19376241267469396 1109 22-23 0.27874055768580064 1109 24-25 0.08840290629131431 1109 26-27 0.12341583104855403 1109 28-29 0.1344650025143963 1109 30-31 0.09079201702055428 1109 32-33 0.05582986902727072 1109 34-35 -0.053347350602770405 1109 36-37 0.11839801334014766 1109 38-39 0.18339683526590989 1109 40-41 0.20822383379407938 1109 42-43 0.29984438152497006 1109 44-45 0.4549850448621555 1109 46-47 0.6323272833585207 1109 48-49 0.5792373313055208 1109 50-51 0.6247631118541932 1109 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-1.0581935543728989 2316 60-61 -1.257436138693599 2316 62-63 -1.3349473652063715 2316 64-65 -1.3876170459532204 2316 66-67 -1.4153757945509398 2316 68-69 -1.4750588953437145 2316 70-71 -1.4706753853698693 2316 72-73 -1.482499687388266 2316 74-75 -1.6183398581026722 2316 76-77 -1.6762540554550895 2316 78-79 -2.4297171823456516 2316 80-81 -2.1823307022918073 2316 82-83 -2.3006792601887014 2316 84-85 -2.4760920296224818 2316 86-87 -2.142386847063701 2316 88-89 -2.315616029730343 2316 90-91 -2.3863022754796397 2316 92-93 -2.6868853529033743 2316 94-95 -2.8232659878671953 2316 96-97 -3.184400202507664 2316 98-99 -3.3070522146380164 2316 100-101 -3.1209169306774687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8202.0 3 2349.0 4 2571.0 5 2845.0 6 3193.0 7 3872.0 8 4928.0 9 6202.0 10 7630.0 11 9805.0 12 12108.0 13 14547.0 14 16977.0 15 20776.0 16 24867.0 17 30325.0 18 30246.0 19 26054.0 20 26372.0 21 28183.0 22 30990.0 23 34977.0 24 38866.0 25 44024.0 26 49837.0 27 57055.0 28 65991.0 29 76849.0 30 90461.0 31 109261.0 32 133156.0 33 167126.0 34 217833.0 35 292623.0 36 380964.0 37 415969.0 38 213214.0 39 12414.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.532642374265464 18.421754031108705 14.813061243829972 23.232542350795857 2 15.402015876474204 19.4236676918948 40.66996317510314 24.504353256527857 3 19.221844025183206 23.70054168368799 29.703932184092817 27.37368210703599 4 12.96550091642857 16.39549893645616 36.625212607372944 34.013787539742324 5 13.955453338786752 37.03075366713517 34.13333608708849 14.880456906989584 6 32.7232581305558 35.66679169114602 17.011479755070038 14.598470423228147 7 28.696947028967262 32.75327043470983 20.215955791295332 18.333826745027572 8 26.54250169764954 34.926862824756114 19.29775925007023 19.232876227524116 9 26.30105613746488 16.882678305918198 20.031108625436005 36.78515693118092 10-11 25.217972547911376 26.355575685882833 26.847939157744893 21.578512608460894 12-13 26.12566694839255 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22.619352866933347 38-39 22.25604755254138 26.35533847384975 28.637976938079003 22.750637035529866 40-41 22.83684693871345 26.159200428013037 28.55223264712533 22.451719986148184 42-43 22.49402235071358 26.073350649810195 28.598412177117556 22.834214822358675 44-45 22.46020080744614 26.402360862229223 28.598309921031856 22.53912840929278 46-47 22.832490058274534 26.54122316627991 28.007366193323136 22.61892058212242 48-49 22.598241866878833 26.66466883000677 27.8871273891795 22.849961913934894 50-51 22.66173573818067 26.847048031034777 27.913697940210774 22.577518290573785 52-53 22.99874651569325 26.612787603884218 27.80768874963743 22.58077713078511 54-55 22.638946503064727 26.830621202954507 27.80177660311406 22.72865569086671 56-57 22.638002519866944 26.862677202907154 27.800578533733766 22.69874174349214 58-59 22.71577705908911 26.578820797744424 27.71497544480379 22.99042669836268 60-61 22.407544289418656 26.803858684444908 27.91475909759106 22.873837928545377 62-63 22.472991381420076 26.74551674622808 28.08168411710418 22.69980775524766 64-65 22.300129552059474 26.771629020398457 27.98818367275481 22.94005775478725 66-67 22.284036689674032 26.74630579679086 27.88284763082863 23.08680988270648 68-69 22.692810693581702 26.48284201489057 27.98739629204499 22.836950999482738 70-71 22.6131337628448 26.578786359816597 27.619307375901165 23.188772501437438 72-73 22.553019988780868 26.610391630319967 27.63411913953226 23.202469241366906 74-75 22.46626293927375 26.987068696249715 27.529065626555415 23.017602737921116 76-77 22.60191000291516 26.624779474010275 27.576795745852344 23.19651477722222 78-79 22.707627736291432 26.817623201182485 27.442458386551603 23.032290675974483 80-81 22.752799463953608 26.81610409560968 27.20785582180143 23.22324061863528 82-83 22.73519343548858 26.800788425419498 27.341614650442125 23.122403488649798 84-85 22.73570342518525 26.884657180181243 27.25212504263722 23.127514351996286 86-87 22.841727182088356 26.811395122540848 27.10681835644592 23.24005933892487 88-89 22.820034712168592 26.824651205738252 26.89466900784277 23.460645074250387 90-91 22.743630211379244 26.807842966678102 27.001423423659205 23.44710339828345 92-93 22.9110970604642 26.406821021889094 27.103618033388983 23.578463884257726 94-95 22.725892902727093 26.424273410951916 27.156746610645733 23.69308707567526 96-97 23.009118507422198 26.02532525551578 27.185264421233907 23.780291815828118 98-99 22.872904391390495 26.210397289077193 26.847545976824023 24.069152342708293 100-101 23.658408633245926 25.25148408594496 26.84540835221482 24.244698928594296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 869.0 1 606.0 2 432.5 3 552.5 4 487.5 5 462.5 6 858.5 7 1898.5 8 3195.0 9 3815.5 10 3293.5 11 2639.5 12 2587.5 13 2768.5 14 2973.5 15 3139.5 16 3312.0 17 3533.5 18 3882.0 19 4201.0 20 4652.5 21 5490.0 22 6605.5 23 8196.5 24 10203.0 25 12921.0 26 16531.0 27 20641.0 28 23994.5 29 26758.5 30 29862.0 31 34525.5 32 40912.5 33 44831.5 34 46956.5 35 50247.0 36 54820.5 37 59969.5 38 64506.5 39 67739.0 40 71287.0 41 76376.0 42 80662.0 43 84304.0 44 87861.0 45 91503.5 46 94300.0 47 98079.0 48 101303.5 49 100815.5 50 99550.0 51 98560.5 52 97754.5 53 98145.5 54 98788.0 55 94682.5 56 87542.0 57 80398.0 58 74813.5 59 67665.5 60 56233.0 61 46494.5 62 38528.5 63 29227.0 64 21211.0 65 16235.5 66 12212.5 67 9116.0 68 7102.0 69 5322.5 70 3792.0 71 2623.5 72 1757.0 73 1211.0 74 850.5 75 614.0 76 434.5 77 274.5 78 180.5 79 127.5 80 78.5 81 49.0 82 33.5 83 26.0 84 19.5 85 15.0 86 12.5 87 11.0 88 11.0 89 10.5 90 6.0 91 2.5 92 2.0 93 1.0 94 1.0 95 3.0 96 3.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4531146472921094 2 0.040351377584975576 3 0.05078008978273639 4 0.05538641142485689 5 0.08155031835210133 6 0.08081330688936204 7 0.046247469286889824 8 0.04009342357301683 9 0.045363055531602685 10-11 0.049250790997552384 12-13 0.04475502107484278 14-15 0.03860097536096979 16-17 0.039024756952044874 18-19 0.04602636584806803 20-21 0.05446514709643279 22-23 0.04621061871375286 24-25 0.06465433056880333 26-27 0.055883894162205905 28-29 0.04398115903896654 30-31 0.04576841183610929 32-33 0.04453391763602099 34-35 0.049048112845299086 36-37 0.04387060731955564 38-39 0.02597965406155962 40-41 0.02433980355696472 42-43 0.023547516234519998 44-45 0.015329838424977024 46-47 0.026808791957141306 48-49 0.026808791957141306 50-51 0.01649063147879139 52-53 0.0156614935832097 54-55 0.01660118319820228 56-57 0.015606217723504253 58-59 0.014261171804005069 60-61 0.01685913721016103 62-63 0.016730160204181655 64-65 0.017209217654962188 66-67 0.009415321436494302 68-69 0.012289666141177493 70-71 0.018498987714755926 72-73 0.01621425218026416 74-75 0.01335833276214945 76-77 0.009691700735021532 78-79 0.014242746517436585 80-81 0.017098665935551295 82-83 0.014961332693607382 84-85 0.013395183335286415 86-87 0.00359293088085399 88-89 0.028761872333400402 90-91 0.030825504429070387 92-93 0.017006539502708886 94-95 0.031931021623179304 96-97 0.026458711512340152 98-99 0.02349224037481455 100-101 0.14731016611501357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2713662.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.74012364572364 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69633530130939 37.61317377080732 2 14.584142111512035 15.091706322328758 3 5.31225776726716 8.24570621149076 4 2.512710668856178 5.200318427701904 5 1.4012596735537184 3.6250674384717874 6 0.8495178127749711 2.6372494003333022 7 0.5371883643519133 1.9455934674828428 8 0.3716977963729906 1.5385351954545232 9 0.2679293795154113 1.2476429302004453 >10 1.3349351400548728 12.188349699413521 >50 0.08422782034031617 2.985444925285305 >100 0.04250827742836318 4.038384289483821 >500 0.0029308831508862427 1.024782297096621 >1k 0.0021445486469482892 1.6455990891019587 >5k 1.4296990979655264E-4 0.5520590668869872 >10k+ 7.148495489827632E-5 0.420387468460237 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11366 0.4188436142747328 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9191 0.3386936177018361 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5735 0.21133803694048853 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.370114627392799E-5 2 7.370114627392799E-5 0.0 0.0 0.0 7.370114627392799E-5 3 7.370114627392799E-5 0.0 0.0 0.0 7.370114627392799E-5 4 7.370114627392799E-5 0.0 0.0 0.0 7.370114627392799E-5 5 1.10551719410892E-4 0.0 0.0 0.0 7.370114627392799E-5 6 1.4740229254785599E-4 0.0 0.0 0.0 1.4740229254785599E-4 7 1.4740229254785599E-4 0.0 0.0 3.6850573136963996E-5 1.4740229254785599E-4 8 1.4740229254785599E-4 0.0 0.0 1.4740229254785599E-4 1.4740229254785599E-4 9 1.4740229254785599E-4 0.0 0.0 1.8425286568482E-4 1.4740229254785599E-4 10-11 1.4740229254785599E-4 0.0 0.0 2.39528725390266E-4 1.8425286568482E-4 12-13 2.57954011958748E-4 0.0 0.0 2.9480458509571197E-4 2.9480458509571197E-4 14-15 2.9480458509571197E-4 0.0 0.0 2.9480458509571197E-4 3.50080444801158E-4 16-17 3.13229871664194E-4 0.0 0.0 2.9480458509571197E-4 4.2378159107508604E-4 18-19 3.3165515823267597E-4 0.0 0.0 3.31655158232676E-4 4.42206877643568E-4 20-21 3.3165515823267597E-4 0.0 0.0 4.05356304506604E-4 4.79057450780532E-4 22-23 3.3165515823267597E-4 0.0 0.0 5.159080239174961E-4 4.79057450780532E-4 24-25 3.6850573136964E-4 0.0 0.0 6.08034456759906E-4 4.79057450780532E-4 26-27 3.6850573136964E-4 0.0 0.0 0.001271344773225258 4.79057450780532E-4 28-29 4.42206877643568E-4 0.0 0.0 0.003758758459970328 4.79057450780532E-4 30-31 4.79057450780532E-4 0.0 0.0 0.011884309836670891 4.79057450780532E-4 32-33 4.974827373490139E-4 0.0 0.0 0.0294804585095712 4.79057450780532E-4 34-35 5.896091701914239E-4 0.0 0.0 0.05674988263092456 5.15908023917496E-4 36-37 5.896091701914239E-4 0.0 0.0 0.09888851301304288 5.15908023917496E-4 38-39 5.896091701914239E-4 0.0 0.0 0.158144234617281 5.71183883622942E-4 40-41 5.896091701914239E-4 0.0 0.0 0.22436471454440532 6.26459743328388E-4 42-43 6.26459743328388E-4 0.0 0.0 0.2920776426835766 6.26459743328388E-4 44-45 7.73862035876244E-4 0.0 0.0 0.37688923675829933 7.00160889602316E-4 46-47 9.765401881295459E-4 0.0 0.0 0.47410104869361036 8.291378955816899E-4 48-49 0.001031816047834992 0.0 0.0 0.5778538373607325 8.84413755287136E-4 50-51 0.001031816047834992 0.0 0.0 0.684867901750476 9.212643284241E-4 52-53 0.001031816047834992 0.0 0.0 0.7867965870473184 0.001031816047834992 54-55 0.001031816047834992 0.0 0.0 0.8995409155598597 0.00110551719410892 56-57 0.00110551719410892 0.0 0.0 1.0309684846528417 0.001142367767245884 58-59 0.00110551719410892 0.0 0.0 1.1660811110595204 0.0011976436269513298 60-61 0.001142367767245884 0.0 0.0 1.3093561394160362 0.001252919486656776 62-63 0.001216068913519812 0.0 0.0 1.4483565012886646 0.0013266206329307039 64-65 0.001252919486656776 0.0 0.0 1.5944137479170215 0.0013266206329307039 66-67 0.001363471206067668 0.0 0.0 1.7606282580512975 0.0013818964926361498 68-69 0.001363471206067668 0.0 0.0 1.9431675720852486 0.001437172352341596 70-71 0.001437172352341596 1.8425286568481998E-5 0.0 2.1302395066150464 0.001437172352341596 72-73 0.001547724071752488 3.6850573136963996E-5 0.0 2.3248105327782165 0.001492448212047042 74-75 0.001584574644889452 3.6850573136963996E-5 0.0 2.517170524553168 0.0015661493583209699 76-77 0.0016029999314579341 3.6850573136963996E-5 0.0 2.7272740672935685 0.001584574644889452 78-79 0.001621425218026416 5.5275859705445994E-5 0.0 2.9574611723936144 0.001639850504594898 80-81 0.001768827510574272 7.370114627392799E-5 0.0 3.202222679169329 0.001713551650868826 82-83 0.001768827510574272 7.370114627392799E-5 0.0 3.448642461736207 0.001879379229985164 84-85 0.001824103370279718 7.370114627392799E-5 0.0 3.7005714049870617 0.001916229803122128 86-87 0.001879379229985164 7.370114627392799E-5 0.0 3.9585806928055156 0.002008356235964538 88-89 0.001879379229985164 7.370114627392799E-5 0.0 4.233836048852067 0.002100482668806948 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5205 0.0 58.12937 1 GTATCAA 9100 0.0 42.531097 1 ATCAACG 11650 0.0 32.966316 3 TATCAAC 11710 0.0 32.918568 2 TCAACGC 11725 0.0 32.795986 4 CAACGCA 11970 0.0 32.12472 5 AACGCAG 12365 0.0 31.091614 6 TGGTATC 2160 0.0 28.606623 2 ACGCAGA 14235 0.0 26.764158 7 CGCAGAG 14380 0.0 26.461248 8 GTGGTAT 2620 0.0 26.047247 1 GCAGAGT 15675 0.0 23.546053 9 TAAGGTG 1535 0.0 23.533699 5 GAGTACT 9620 0.0 23.13445 12-13 GTACATG 9170 0.0 22.638466 1 GTAAGGT 1515 0.0 22.589407 4 TACATGG 8890 0.0 22.241728 2 CAGAGTA 14765 0.0 21.8927 10-11 GTACTTT 10215 0.0 21.671265 14-15 ACATGGG 9000 0.0 21.178093 3 >>END_MODULE