FastQCFastQC Report
Thu 26 May 2016
SRR938138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713662
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110080.40565110909169966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91820.33836196254360346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60980.2247147949892065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50950.054.6357881
GTATCAA92000.042.8562361
TCAACGC117850.033.4509354
TATCAAC117950.033.3469542
ATCAACG117750.033.3210683
CAACGCA121200.032.4479645
AACGCAG125450.031.3108316
TGGTATC22050.029.2990842
ACGCAGA144600.026.9671237
CGCAGAG144850.026.7566288
GCAGAGT160250.023.8889339
GTGGTAT29550.022.5057931
GAGTACT100100.022.32468412-13
TACATGG90600.022.1786942
GTACATG96100.021.6013951
GTACTTT102950.021.5221214-15
CAGAGTA152300.021.20648810-11
AGAGTAC143750.021.12965610-11
TAAGGTG14550.020.8918425
ACATGGG90600.020.8143123
Produced by FastQC (version 0.11.5)