##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938138_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2713662 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.483108065779746 33.0 31.0 34.0 31.0 34.0 2 32.714087458202236 34.0 31.0 34.0 31.0 34.0 3 32.68065182767788 34.0 31.0 34.0 31.0 34.0 4 36.21283159066973 37.0 35.0 37.0 35.0 37.0 5 36.17344459258375 37.0 35.0 37.0 35.0 37.0 6 36.22978138028981 37.0 37.0 37.0 35.0 37.0 7 36.189710435566404 37.0 37.0 37.0 35.0 37.0 8 36.18033196470305 37.0 37.0 37.0 35.0 37.0 9 37.88712485195282 39.0 38.0 39.0 35.0 39.0 10-11 37.85111668291777 39.0 38.0 39.0 35.0 39.0 12-13 37.79468426797442 39.0 38.0 39.0 35.0 39.0 14-15 39.06993888700951 40.0 38.0 41.0 36.0 41.0 16-17 39.00202014841936 40.0 38.0 41.0 36.0 41.0 18-19 38.993917444398015 40.0 38.5 41.0 36.0 41.0 20-21 38.99568811443724 40.0 38.5 41.0 35.5 41.0 22-23 39.02353167048807 40.0 39.0 41.0 35.0 41.0 24-25 38.976551243301486 40.0 38.0 41.0 35.0 41.0 26-27 38.811261129794346 40.0 38.0 41.0 35.0 41.0 28-29 38.729332540309 40.0 38.0 41.0 35.0 41.0 30-31 38.520816520259345 40.0 38.0 41.0 34.0 41.0 32-33 38.326154104674785 40.0 38.0 41.0 34.0 41.0 34-35 38.1307922652121 40.0 38.0 41.0 34.0 41.0 36-37 38.057902384305784 40.0 38.0 41.0 33.5 41.0 38-39 38.01930509400212 40.0 38.0 41.0 33.0 41.0 40-41 37.90250904497317 40.0 37.5 41.0 33.0 41.0 42-43 37.72150861087343 40.0 37.0 41.0 33.0 41.0 44-45 37.47697594615689 40.0 37.0 41.0 33.0 41.0 46-47 37.137495384465716 40.0 37.0 41.0 31.5 41.0 48-49 36.770473441423434 40.0 36.0 41.0 31.0 41.0 50-51 36.47590193620282 39.0 36.0 41.0 30.0 41.0 52-53 36.007980175865676 39.0 35.0 40.0 29.5 41.0 54-55 35.69348393425563 39.0 35.0 40.0 28.5 41.0 56-57 35.416537321154955 38.0 35.0 40.0 28.0 41.0 58-59 35.030043535267104 38.0 34.0 40.0 27.5 41.0 60-61 34.611167123982284 37.5 34.0 40.0 26.0 41.0 62-63 34.17130928612333 37.0 33.5 40.0 26.0 41.0 64-65 33.7312837781566 36.0 33.0 39.0 25.0 41.0 66-67 33.21455011714797 36.0 32.5 39.0 23.5 40.0 68-69 32.67846437765647 35.0 32.0 38.5 22.0 40.0 70-71 32.08954136513685 35.0 31.0 37.5 20.5 40.0 72-73 31.61903380008269 35.0 31.0 37.0 19.5 39.0 74-75 31.065973765339972 34.5 30.5 36.5 18.0 39.0 76-77 29.860571987226116 33.5 29.0 35.0 17.5 37.5 78-79 30.56499869917477 34.0 30.0 36.0 17.5 37.5 80-81 30.388009818466706 34.0 30.0 35.0 14.0 37.0 82-83 29.93178700958336 34.0 30.0 35.0 7.5 37.0 84-85 29.421212553368843 34.0 29.0 35.0 4.5 36.0 86-87 28.88651644899033 34.0 29.0 35.0 2.0 36.0 88-89 28.272105737560537 33.5 28.0 35.0 2.0 35.0 90-91 27.74728558678273 33.0 27.0 35.0 2.0 35.0 92-93 27.11209962773551 33.0 25.5 35.0 2.0 35.0 94-95 26.368095952996356 32.0 25.0 34.5 2.0 35.0 96-97 25.54043779217898 32.0 22.0 34.0 2.0 35.0 98-99 24.50587011204785 32.0 10.0 34.0 2.0 35.0 100-101 20.998238911109787 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.022092563202903648 1101 2 0.05567192457417747 1101 3 0.22126245718276039 1101 4 0.06776442815428396 1101 5 0.04548473755286153 1101 6 0.07570946848301219 1101 7 0.06075570035266509 1101 8 0.10216667789243417 1101 9 0.0354445926340361 1101 10-11 0.18059331778535892 1101 12-13 0.09061307731349189 1101 14-15 0.1562685739678571 1101 16-17 0.22112551678405623 1101 18-19 0.25377973718671143 1101 20-21 0.21004016530112324 1101 22-23 0.20309682869596912 1101 24-25 0.20488212748628598 1101 26-27 0.22151044547707244 1101 28-29 0.1857931387830476 1101 30-31 0.08652047884857694 1101 32-33 0.04933521380203132 1101 34-35 -0.03525202876119948 1101 36-37 0.16052186732861884 1101 38-39 0.24367480560152188 1101 40-41 0.2869775322655741 1101 42-43 0.1803947351010322 1101 44-45 0.21020444188768295 1101 46-47 0.13791227040019294 1101 48-49 0.15015816470097576 1101 50-51 0.16548297987664995 1101 52-53 0.1767683316350812 1101 54-55 0.25059717700235495 1101 56-57 0.2395386310139287 1101 58-59 0.21628623520737023 1101 60-61 0.294718374357231 1101 62-63 0.3841498852037901 1101 64-65 0.16272019503357882 1101 66-67 0.23769580401015133 1101 68-69 0.4756457325109906 1101 70-71 0.6417598685775872 1101 72-73 0.6382552628054476 1101 74-75 0.44657687526921563 1101 76-77 0.558921763143065 1101 78-79 0.644761308750514 1101 80-81 0.7974240190427508 1101 82-83 0.7957949705513769 1101 84-85 0.8869404255691755 1101 86-87 0.9900354286466957 1101 88-89 1.2551471410356108 1101 90-91 1.3800426809683728 1101 92-93 1.6289882351424225 1101 94-95 1.588860164431015 1101 96-97 1.9387598857103754 1101 98-99 1.885490598365248 1101 100-101 2.1818651512933833 1102 1 0.12397995153800423 1102 2 0.16150016649522314 1102 3 0.28717425032115784 1102 4 0.18218699317857556 1102 5 0.08354426196688536 1102 6 0.09542074700314629 1102 7 0.1771646906195059 1102 8 0.1658333634013971 1102 9 0.1781045757142934 1102 10-11 0.2057920960380244 1102 12-13 0.24844510174587953 1102 14-15 0.2718718963439173 1102 16-17 0.31254922160765375 1102 18-19 0.37658680515598775 1102 20-21 0.38159057978375444 1102 22-23 0.3612682540007839 1102 24-25 0.29888379391183406 1102 26-27 0.2541042665448643 1102 28-29 0.11002610558579562 1102 30-31 0.23085247629496308 1102 32-33 0.2713120071176789 1102 34-35 0.2643514366885711 1102 36-37 0.20598130481258892 1102 38-39 0.30593718107868284 1102 40-41 0.4051656026642263 1102 42-43 0.24211244044135327 1102 44-45 0.2458612540328602 1102 46-47 0.3724411474982787 1102 48-49 0.4426998405093556 1102 50-51 0.48209121945357936 1102 52-53 0.4991890207330201 1102 54-55 0.5127724148384587 1102 56-57 0.5267812300629302 1102 58-59 0.4671910646797883 1102 60-61 0.5838565404524019 1102 62-63 0.5620357826384605 1102 64-65 0.5855423049046138 1102 66-67 0.6491469866517363 1102 68-69 0.7151140060743231 1102 70-71 0.9436102779823798 1102 72-73 0.9121426650690161 1102 74-75 0.9164079620577965 1102 76-77 0.9090114876080015 1102 78-79 0.9392143599731391 1102 80-81 0.9407913068345692 1102 82-83 0.9895344256513035 1102 84-85 0.9683999882426484 1102 86-87 1.0093723362680187 1102 88-89 1.2805400109186884 1102 90-91 1.2755755555077357 1102 92-93 1.5853163408560107 1102 94-95 1.6541901617196402 1102 96-97 1.9753263177425353 1102 98-99 2.167824379762024 1102 100-101 2.584411751647938 1103 1 0.22200331942022444 1103 2 0.2429905512033912 1103 3 0.32395892205263266 1103 4 0.21128510532140155 1103 5 0.14213842960718637 1103 6 0.17334894042136995 1103 7 0.22265181612913665 1103 8 0.1939234939044283 1103 9 0.2455073410925266 1103 10-11 0.2793148326531707 1103 12-13 0.29942681758036116 1103 14-15 0.42593030099634177 1103 16-17 0.33826278885139516 1103 18-19 0.3526395698973559 1103 20-21 0.36946421134688734 1103 22-23 0.43839351661059567 1103 24-25 0.4115538703084596 1103 26-27 0.4911810464536259 1103 28-29 0.4526407632560847 1103 30-31 0.42512988874452873 1103 32-33 0.5253008006313991 1103 34-35 0.45876056414275723 1103 36-37 0.5175809869210823 1103 38-39 0.55881734917984 1103 40-41 0.6369198419574857 1103 42-43 0.5335796047109085 1103 44-45 0.5441805898727239 1103 46-47 0.44419919253496687 1103 48-49 0.5066361371504584 1103 50-51 0.5251875970111257 1103 52-53 0.5697744644977547 1103 54-55 0.6251873597536033 1103 56-57 0.5281357234841337 1103 58-59 0.6764492496335208 1103 60-61 0.7894237139468174 1103 62-63 0.7098199213342653 1103 64-65 0.6377066574399706 1103 66-67 0.6431864243434902 1103 68-69 0.7895694586123412 1103 70-71 0.6976463538054531 1103 72-73 0.6718299418048481 1103 74-75 0.6496226457430048 1103 76-77 0.4065219292788065 1103 78-79 0.8891856239083928 1103 80-81 0.9360238026341676 1103 82-83 1.2018801454057702 1103 84-85 1.310025287713355 1103 86-87 1.37200902556458 1103 88-89 1.572741980873385 1103 90-91 1.624487348333254 1103 92-93 1.8680619454346399 1103 94-95 1.7725116320180483 1103 96-97 1.9135739813209902 1103 98-99 2.3668620593238288 1103 100-101 2.7014830390892577 1104 1 0.07822786559123074 1104 2 0.08708901150054515 1104 3 0.16910574763716824 1104 4 0.09858806825197064 1104 5 0.03608145587165268 1104 6 0.08045660002128585 1104 7 0.006940795798207944 1104 8 0.001834876423878029 1104 9 -0.007781318158414763 1104 10-11 0.05302831815090059 1104 12-13 2.4839109222085654E-4 1104 14-15 0.08108131254477513 1104 16-17 0.14315652873791862 1104 18-19 0.10657700892642197 1104 20-21 0.11493270460515959 1104 22-23 0.11900402471877669 1104 24-25 0.08128944055902565 1104 26-27 0.12629878449796905 1104 28-29 0.06692498574229688 1104 30-31 0.135330520476991 1104 32-33 0.16173475210099042 1104 34-35 0.18404931978690087 1104 36-37 0.3592717593251322 1104 38-39 0.3417695872424744 1104 40-41 0.26009267764010957 1104 42-43 0.26140207342997 1104 44-45 0.24639834746346878 1104 46-47 0.44887988560206793 1104 48-49 0.3722531294863032 1104 50-51 0.4055677432253191 1104 52-53 0.37872546551434993 1104 54-55 0.5051576563679774 1104 56-57 0.6618829823025507 1104 58-59 0.6069315458623166 1104 60-61 0.6689935325223288 1104 62-63 0.5923775720535005 1104 64-65 0.5691950130523296 1104 66-67 0.5735281471577096 1104 68-69 0.7450640940630748 1104 70-71 0.7292259259446041 1104 72-73 0.7026587180307153 1104 74-75 0.7748539224798456 1104 76-77 0.7823054587157721 1104 78-79 0.7810829722921575 1104 80-81 0.9832278197314608 1104 82-83 0.9916561293973594 1104 84-85 1.3141164649688442 1104 86-87 1.3870962569466379 1104 88-89 1.484848431095667 1104 90-91 1.3551269975334144 1104 92-93 1.7053098839143566 1104 94-95 1.642264249584283 1104 96-97 2.0953879763638845 1104 98-99 2.3484767525033874 1104 100-101 2.8747812305564935 1105 1 0.061437580371645595 1105 2 0.06943233793709425 1105 3 0.14756904479299493 1105 4 0.09064416957127008 1105 5 0.053104382405798845 1105 6 0.018850104782750066 1105 7 0.06694487370630497 1105 8 0.013544489256588577 1105 9 -0.01122547922909689 1105 10-11 -0.018106109086851063 1105 12-13 -0.010898670975542757 1105 14-15 0.05816647731566604 1105 16-17 0.04501966478294861 1105 18-19 0.04420273049888834 1105 20-21 0.11314544439117213 1105 22-23 -0.004196264077336309 1105 24-25 0.03079591340249266 1105 26-27 0.10323487164487233 1105 28-29 0.0981290048215584 1105 30-31 0.058097949355939704 1105 32-33 0.025604486335033982 1105 34-35 0.056154088599591034 1105 36-37 0.0844812534974082 1105 38-39 0.07930539428388528 1105 40-41 0.10664600965870363 1105 42-43 0.07643919242132569 1105 44-45 0.13031434255660912 1105 46-47 0.030206074796943483 1105 48-49 0.07309144043014015 1105 50-51 0.13770734138424956 1105 52-53 0.1299329608431421 1105 54-55 0.19213041126229768 1105 56-57 0.19815798038057864 1105 58-59 0.19797251546852834 1105 60-61 0.23486043896107844 1105 62-63 0.18777555248623656 1105 64-65 0.31803999572122166 1105 66-67 0.3685728203066887 1105 68-69 0.4479059280521085 1105 70-71 0.5391603117347685 1105 72-73 0.46962398561836594 1105 74-75 0.4507167175850171 1105 76-77 0.4318995063069657 1105 78-79 0.5986340213507297 1105 80-81 0.6393054069468853 1105 82-83 0.7160978403806375 1105 84-85 0.8478090107533909 1105 86-87 0.9566513304325461 1105 88-89 1.0513006176231947 1105 90-91 1.067459661243042 1105 92-93 1.1649646655958605 1105 94-95 1.2939664419612704 1105 96-97 1.375405892419142 1105 98-99 1.6406190915513648 1105 100-101 2.004665277579452 1106 1 0.0500279223435669 1106 2 0.0725498059801879 1106 3 0.11555143379676025 1106 4 0.06837474312209224 1106 5 0.025719646479934966 1106 6 -0.024190594053131065 1106 7 0.025691607966543017 1106 8 -0.07782020728581074 1106 9 -0.027164494538830297 1106 10-11 0.010940230041576626 1106 12-13 0.04460100342338791 1106 14-15 0.06004867199656161 1106 16-17 0.024030395348717093 1106 18-19 0.02690170388262203 1106 20-21 -0.04191609550728259 1106 22-23 0.019525005844649 1106 24-25 -0.031763596353862056 1106 26-27 -0.026041917798913516 1106 28-29 -0.09049869695380863 1106 30-31 0.06004364273054108 1106 32-33 0.10511282872725047 1106 34-35 -0.024399485099110052 1106 36-37 0.09837657080925766 1106 38-39 0.2148254219924084 1106 40-41 0.15090602610739268 1106 42-43 0.16621103817723082 1106 44-45 0.35840995252026175 1106 46-47 0.039272961817268026 1106 48-49 0.21411341266453832 1106 50-51 0.2917966832031027 1106 52-53 0.27988139653108846 1106 54-55 0.3360358987428711 1106 56-57 0.39266628385634306 1106 58-59 0.3953727660905457 1106 60-61 0.35685894197450807 1106 62-63 0.4359444032312254 1106 64-65 0.4863350136357596 1106 66-67 0.4412485994791169 1106 68-69 0.10884450230860665 1106 70-71 0.29277441869276544 1106 72-73 0.46343821399573315 1106 74-75 0.6467371850495596 1106 76-77 0.6738859581893557 1106 78-79 0.8310463749924004 1106 80-81 1.0458415564483658 1106 82-83 0.8837457423941757 1106 84-85 1.0060687054926625 1106 86-87 1.0974354582238277 1106 88-89 1.3197476691823198 1106 90-91 1.2302482592385822 1106 92-93 1.0737937081730635 1106 94-95 1.4929630458513579 1106 96-97 1.7519142296225034 1106 98-99 2.005200246643838 1106 100-101 2.197860734212245 1107 1 -0.012599298342536258 1107 2 0.035742476682948165 1107 3 0.10574767719538869 1107 4 -0.005351144645075578 1107 5 0.08374885855177894 1107 6 0.02645901400941142 1107 7 0.007945667769483578 1107 8 0.011982935637362857 1107 9 0.010825773712774378 1107 10-11 0.016658201360037594 1107 12-13 -0.011907499236450292 1107 14-15 0.07180945578239317 1107 16-17 0.03179286773938372 1107 18-19 0.019988606653733143 1107 20-21 -0.04462636318480406 1107 22-23 -0.01300624843934628 1107 24-25 0.03444436235945858 1107 26-27 0.0410353977351221 1107 28-29 0.011032999277460931 1107 30-31 -0.060940167547876456 1107 32-33 -0.09565650844585605 1107 34-35 0.048150268613021296 1107 36-37 0.01190269395170418 1107 38-39 -0.08370136679291562 1107 40-41 -0.007535971157778931 1107 42-43 -0.1226093040671472 1107 44-45 -0.13680632697649742 1107 46-47 -0.00259659395396028 1107 48-49 -0.03902847121863573 1107 50-51 -0.01351353854753512 1107 52-53 -0.06843325261077382 1107 54-55 -0.18114764745595835 1107 56-57 -0.0852868939821505 1107 58-59 -0.08295412444658723 1107 60-61 -0.048255706969399625 1107 62-63 0.0035275702363222194 1107 64-65 0.00274645121157846 1107 66-67 0.09021731079945283 1107 68-69 0.14251372134867069 1107 70-71 0.2150710672674805 1107 72-73 0.17880474072067543 1107 74-75 0.26172618833223993 1107 76-77 0.31052635711977317 1107 78-79 0.567932220455365 1107 80-81 0.65783733196686 1107 82-83 0.7473292246517005 1107 84-85 0.8834968172988944 1107 86-87 0.8924661371845168 1107 88-89 0.8367089426239609 1107 90-91 0.9823856162552786 1107 92-93 1.0838911271118619 1107 94-95 1.0626653822331242 1107 96-97 0.9811095490395267 1107 98-99 1.2266248040655583 1107 100-101 1.904372478478681 1108 1 0.0780552949593627 1108 2 0.06704513503784426 1108 3 0.12343994002812053 1108 4 0.08298586127095575 1108 5 0.013915365494739262 1108 6 0.0352980033089878 1108 7 0.03578375019730373 1108 8 0.08087791913258258 1108 9 0.022822641097711482 1108 10-11 0.04255882228741115 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-3.090801160022089 2316 98-99 -3.3878393171285666 2316 100-101 -4.024855518080582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 12.0 4 11.0 5 33.0 6 89.0 7 235.0 8 541.0 9 1135.0 10 1849.0 11 3210.0 12 4807.0 13 7110.0 14 9530.0 15 13145.0 16 16993.0 17 23768.0 18 24295.0 19 18559.0 20 16862.0 21 17020.0 22 18156.0 23 19828.0 24 22707.0 25 26431.0 26 30604.0 27 37204.0 28 45043.0 29 54711.0 30 66624.0 31 82789.0 32 104616.0 33 136754.0 34 189315.0 35 280366.0 36 431756.0 37 597543.0 38 383713.0 39 26291.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.811411295879886 18.79976209269983 15.141568846820274 23.24725776460001 2 15.537991617859296 19.659186227697624 40.25904146402947 24.54378069041361 3 19.552076158551124 23.65442084513084 29.781643392272038 27.011859604045995 4 13.194863619713878 16.740994272683924 36.313549734639025 33.750592372963176 5 13.8778981689995 37.129102357596935 34.21222241753091 14.780777055872656 6 32.80910801115101 35.87309366887095 16.79811914189534 14.519679178082697 7 28.753286149859488 32.711185107061965 20.062483831811036 18.473044911267504 8 26.572083459239547 35.11578458613091 19.168245100712692 19.14388685391685 9 26.163501570939935 16.841375233909012 19.956169928310896 37.03895326684016 10-11 25.230831990129943 26.43540352483102 26.773194303491003 21.560570181548034 12-13 26.067948034795783 23.57078737145599 27.398143173320776 22.963121420427452 14-15 22.719741810144374 25.01691441306987 25.792840081041778 26.47050369574398 16-17 22.32286936356457 28.300826816614162 26.572792253711867 22.8035115661094 18-19 22.362991411605424 26.692436272461347 28.913328188993326 22.031244126939907 20-21 22.83899026481559 25.812260333084964 29.341494998271706 22.00725440382774 22-23 22.673217961558954 25.675249902161728 29.23378077299236 22.417751363286953 24-25 22.53092095298777 25.898037045076066 29.235266782698933 22.33577521923723 26-27 22.092942252312056 26.26081757837365 29.123248611070668 22.52299155824362 28-29 22.24171830528655 26.58019151692526 28.947334964469444 22.230755213318737 30-31 22.380598000816978 26.19091532703913 28.837637455118248 22.59084921702565 32-33 22.130485261864 26.119108306426753 28.878753355146042 22.871653076563202 34-35 22.67804631827556 26.276907517694475 28.787996338648142 22.257049825381824 36-37 22.723259867426368 25.957149156484466 28.795672497098234 22.523918478990932 38-39 22.26898956553181 26.292688869144996 28.69206920338192 22.74625236194127 40-41 22.76241403682076 26.115998573538775 28.681527787888534 22.44005960175193 42-43 22.479904139918567 26.0891423567176 28.66634035120439 22.76461315215945 44-45 22.45072076824945 26.332968414597314 28.679387902105468 22.536922915047768 46-47 22.743435393139386 26.53790805957389 28.060826833676973 22.657829713609758 48-49 22.55871007802215 26.618975716308558 27.96590573037631 22.856408475292987 50-51 22.6757053752457 26.81472489941636 27.976310240553172 22.533259484784768 52-53 23.06105181853893 26.553749140460383 27.836941372949177 22.548257668051512 54-55 22.65195518085893 26.800795382770588 27.83180071799657 22.715448718373917 56-57 22.609411194172303 26.856660851646225 27.83977886708072 22.69414908710075 58-59 22.678763972816068 26.607053494503003 27.731088101613246 22.983094431067684 60-61 22.389763352989426 26.861930483604812 27.921347610719387 22.826958552686367 62-63 22.419188535639297 26.820141933667497 28.053401639555698 22.707267891137512 64-65 22.25525876103951 26.885901781430405 27.946461276312228 22.912378181217854 66-67 22.252302614850677 26.829138865898155 27.81686611483999 23.10169240441117 68-69 22.698719348333384 26.553608920801537 27.909233202789963 22.838438528075116 70-71 22.542158898197343 26.7363068797809 27.527580811464357 23.193953410557395 72-73 22.54647041525437 26.731479454699958 27.55245494833181 23.169595181713863 74-75 22.46622829224863 27.126959805605853 27.376990944340157 23.02982095780536 76-77 22.507666761741145 26.71533890366597 27.584109590656464 23.192884743936425 78-79 22.637159675744435 26.932259802436707 27.48059633071473 22.949984191104125 80-81 22.69076379218155 26.94080349874726 27.27964514306709 23.088787566004104 82-83 22.688103571662836 26.92982630697241 27.39239721731491 22.98967290404984 84-85 22.67262464341137 27.018979857095527 27.298980933172317 23.00941456632079 86-87 22.753572505142536 26.97402605059916 27.20845269936123 23.06394874489708 88-89 22.615956337958877 27.11259307765541 26.98383715139121 23.287613432994497 90-91 22.561364465113467 27.001617615815967 27.120649375368867 23.3163685437017 92-93 22.682818119456606 26.698650690132265 27.20363540350595 23.414895786905184 94-95 22.505347483720783 26.72166496938912 27.257270118082243 23.51571742880785 96-97 22.70333902492638 26.408678490955822 27.318301557882435 23.56968092623537 98-99 22.557043483996758 26.615483516963383 27.047041930740235 23.780431068299617 100-101 22.843019239638405 26.258770437175393 27.187189813643585 23.711020509542617 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 861.0 1 557.0 2 387.5 3 542.0 4 460.5 5 428.0 6 812.0 7 2075.5 8 3455.0 9 3957.5 10 3389.0 11 2667.5 12 2632.5 13 2763.0 14 2939.5 15 3163.5 16 3365.5 17 3522.0 18 3769.5 19 4087.5 20 4602.0 21 5295.5 22 6336.5 23 7978.0 24 9995.0 25 12644.5 26 16306.0 27 20626.5 28 23840.0 29 26685.5 30 30072.0 31 34391.5 32 40943.5 33 45033.5 34 46855.5 35 50208.5 36 54930.0 37 59761.5 38 63977.5 39 67600.0 40 71680.5 41 77082.5 42 81427.5 43 85227.5 44 89074.0 45 92667.5 46 94685.0 47 98857.5 48 102909.0 49 102418.0 50 101110.0 51 99666.0 52 98782.0 53 99422.0 54 100642.0 55 95933.0 56 88036.5 57 80667.0 58 74486.5 59 67275.5 60 55150.0 61 45230.0 62 37464.5 63 27581.5 64 19323.0 65 14588.0 66 10841.5 67 7985.5 68 6090.5 69 4537.5 70 3214.0 71 2056.0 72 1313.0 73 900.0 74 597.0 75 440.5 76 332.0 77 203.0 78 115.0 79 69.0 80 36.0 81 21.0 82 13.5 83 9.5 84 5.0 85 1.5 86 2.0 87 2.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010097057039528136 3 0.00608034456759906 4 0.0 5 3.31655158232676E-4 6 2.5795401195874803E-4 7 0.0 8 7.370114627392799E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.6850573136963996E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001805678083711236 26-27 0.022147194455315364 28-29 4.23781591075086E-4 30-31 1.2897700597937401E-4 32-33 0.003924586039086666 34-35 0.016822286637024064 36-37 0.008346654815522346 38-39 0.021612861144829385 40-41 0.024118700118142936 42-43 0.019936160067097523 44-45 0.01004178117982269 46-47 0.0021004826688069476 48-49 3.50080444801158E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.9480458509571197E-4 68-69 1.65827579116338E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8425286568481998E-4 82-83 8.844137552871359E-4 84-85 0.003721907886833364 86-87 7.370114627392799E-4 88-89 1.8425286568481998E-5 90-91 0.0037034826002648818 92-93 5.711838836229419E-4 94-95 9.212643284240999E-5 96-97 3.86931017938122E-4 98-99 3.13229871664194E-4 100-101 0.0026348159792929263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2713662.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.62232801592634 #Duplication Level Percentage of deduplicated Percentage of total 1 70.74320438035002 35.10442492659446 2 15.404488227292912 15.288131354644094 3 5.742970687375729 8.549387257044252 4 2.727571170762754 5.413937252894945 5 1.5327158290012177 3.802846381095045 6 0.9174105887067624 2.7314429494854635 7 0.6055876140728284 2.1035467059532875 8 0.4089216215377704 1.6233314269401384 9 0.322419050233958 1.4399265482363608 >10 1.4490933496117262 12.638528021254583 >50 0.09255356806699616 3.172423217080721 >100 0.0471816127760561 4.301360642109444 >500 0.003350677335615584 1.1215358470572034 >1k 0.0023082443867121225 1.7378743679760893 >5k 1.4891899269110466E-4 0.564573622678317 >10k+ 7.445949634555233E-5 0.40672947895568806 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11008 0.40565110909169966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9182 0.33836196254360346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6098 0.2247147949892065 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.370114627392799E-5 0.0 2 0.0 0.0 0.0 7.370114627392799E-5 0.0 3 0.0 0.0 0.0 1.10551719410892E-4 0.0 4 0.0 0.0 0.0 1.10551719410892E-4 0.0 5 3.6850573136963996E-5 0.0 0.0 1.10551719410892E-4 3.6850573136963996E-5 6 3.6850573136963996E-5 0.0 0.0 1.4740229254785599E-4 7.370114627392799E-5 7 7.370114627392799E-5 0.0 0.0 1.4740229254785599E-4 7.370114627392799E-5 8 7.370114627392799E-5 0.0 0.0 1.8425286568482E-4 7.370114627392799E-5 9 7.370114627392799E-5 0.0 0.0 1.8425286568482E-4 7.370114627392799E-5 10-11 7.370114627392799E-5 0.0 0.0 2.21103438821784E-4 1.10551719410892E-4 12-13 1.28977005979374E-4 0.0 0.0 2.21103438821784E-4 2.57954011958748E-4 14-15 1.4740229254785599E-4 0.0 0.0 2.21103438821784E-4 3.31655158232676E-4 16-17 1.8425286568482E-4 0.0 0.0 2.57954011958748E-4 4.974827373490139E-4 18-19 2.02678152253302E-4 0.0 0.0 3.6850573136963996E-4 5.5275859705446E-4 20-21 2.21103438821784E-4 0.0 0.0 5.34333310485978E-4 6.26459743328388E-4 22-23 2.21103438821784E-4 0.0 0.0 6.63310316465352E-4 7.00160889602316E-4 24-25 3.3165515823267597E-4 0.0 0.0 7.554367493077621E-4 7.00160889602316E-4 26-27 3.3165515823267597E-4 0.0 0.0 0.0014187470657731139 7.00160889602316E-4 28-29 5.15908023917496E-4 0.0 0.0 0.0038877354659497022 7.00160889602316E-4 30-31 5.15908023917496E-4 0.0 0.0 0.012031712129218745 7.00160889602316E-4 32-33 5.71183883622942E-4 0.0 0.0 0.029830538954372356 7.00160889602316E-4 34-35 6.81735603033834E-4 0.0 0.0 0.057376342374252945 7.00160889602316E-4 36-37 8.47563182150172E-4 0.0 0.0 0.09988347848774093 7.00160889602316E-4 38-39 9.76540188129546E-4 0.0 0.0 0.1594892805367802 7.00160889602316E-4 40-41 0.0010318160478349921 0.0 0.0 0.22609669148184264 7.00160889602316E-4 42-43 0.0011792183403828479 0.0 0.0 0.29443607936434235 7.00160889602316E-4 44-45 0.0013818964926361498 0.0 0.0 0.37906342057338016 7.370114627392799E-4 46-47 0.001621425218026416 0.0 0.0 0.47657003709378687 8.291378955816899E-4 48-49 0.0016582757911633799 0.0 0.0 0.5804886533400254 9.94965474698028E-4 50-51 0.0016582757911633799 0.0 0.0 0.6877606717417275 0.001031816047834992 52-53 0.0016582757911633799 0.0 0.0 0.7904263685013093 0.001142367767245884 54-55 0.0016582757911633799 0.0 0.0 0.9033733751661039 0.001142367767245884 56-57 0.0016582757911633799 0.0 0.0 1.0357774844472156 0.001216068913519812 58-59 0.0016582757911633799 0.0 0.0 1.1716086970300648 0.001216068913519812 60-61 0.001695126364300344 0.0 0.0 1.3150679782522658 0.00128977005979374 62-63 0.001695126364300344 0.0 0.0 1.4544368458562635 0.0013266206329307039 64-65 0.001695126364300344 0.0 0.0 1.6005862189174629 0.0013266206329307039 66-67 0.001768827510574272 0.0 0.0 1.766616476186054 0.0013266206329307039 68-69 0.001768827510574272 0.0 0.0 1.9501691809812718 0.0013266206329307039 70-71 0.001787252797142754 0.0 0.0 2.138291356845473 0.001345045919499186 72-73 0.0018609539434166818 0.0 0.0 2.3334151416056974 0.001400321779204632 74-75 0.001916229803122128 0.0 0.0 2.5273412827389707 0.0014740229254785599 76-77 0.001916229803122128 0.0 0.0 2.739821687446705 0.001510873498615524 78-79 0.001916229803122128 0.0 0.0 2.973970229158974 0.001547724071752488 80-81 0.002045206809101502 0.0 0.0 3.222859000126029 0.001547724071752488 82-83 0.002063632095669984 0.0 0.0 3.473350771024542 0.001584574644889452 84-85 0.0020820573822384657 0.0 0.0 3.730641472666824 0.001584574644889452 86-87 0.002100482668806948 0.0 0.0 3.9952101625036573 0.0016582757911633799 88-89 0.002100482668806948 0.0 0.0 4.278277840055246 0.00175040222400579 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5095 0.0 54.635788 1 GTATCAA 9200 0.0 42.856236 1 TCAACGC 11785 0.0 33.450935 4 TATCAAC 11795 0.0 33.346954 2 ATCAACG 11775 0.0 33.321068 3 CAACGCA 12120 0.0 32.447964 5 AACGCAG 12545 0.0 31.310831 6 TGGTATC 2205 0.0 29.299084 2 ACGCAGA 14460 0.0 26.967123 7 CGCAGAG 14485 0.0 26.756628 8 GCAGAGT 16025 0.0 23.888933 9 GTGGTAT 2955 0.0 22.505793 1 GAGTACT 10010 0.0 22.324684 12-13 TACATGG 9060 0.0 22.178694 2 GTACATG 9610 0.0 21.601395 1 GTACTTT 10295 0.0 21.52212 14-15 CAGAGTA 15230 0.0 21.206488 10-11 AGAGTAC 14375 0.0 21.129656 10-11 TAAGGTG 1455 0.0 20.891842 5 ACATGGG 9060 0.0 20.814312 3 >>END_MODULE