FastQCFastQC Report
Thu 26 May 2016
SRR938137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742455
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116430.4245466197257567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92850.33856526360505457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59550.21714121106818526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55950.056.418061
GTATCAA96050.040.7195851
ATCAACG123700.031.3093953
TATCAAC124650.031.1845782
TCAACGC125150.030.8327274
CAACGCA128350.030.0269855
AACGCAG133100.029.0268066
TGGTATC24400.028.4342562
ACGCAGA149150.025.8208527
CGCAGAG149300.025.6038368
GTGGTAT30950.023.6073671
GCAGAGT165550.022.7738769
TAAGGTG14850.022.4005345
GAGTACT103050.022.14230212-13
TACATGG91750.021.7013532
CAGAGTA156200.021.51906410-11
GTACATG96100.021.4175951
AGAGTAC147350.021.00467110-11
ACATGGG90950.021.004393
GTACTTT108950.020.84320814-15
Produced by FastQC (version 0.11.5)