##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938137_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2742455 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85206138295797 33.0 31.0 34.0 30.0 34.0 2 32.048023759733525 34.0 31.0 34.0 30.0 34.0 3 32.11910532716125 34.0 31.0 34.0 30.0 34.0 4 35.63864748920219 37.0 35.0 37.0 33.0 37.0 5 35.65759438167627 37.0 35.0 37.0 35.0 37.0 6 35.67999730168772 37.0 35.0 37.0 35.0 37.0 7 35.61575048633433 37.0 35.0 37.0 33.0 37.0 8 35.601183611034635 37.0 35.0 37.0 33.0 37.0 9 37.26209545826641 39.0 37.0 39.0 34.0 39.0 10-11 37.18139422524709 39.0 37.0 39.0 34.0 39.0 12-13 37.0820193950311 39.0 37.0 39.0 33.0 39.0 14-15 38.33209004341001 40.0 38.0 41.0 33.5 41.0 16-17 38.252135951182424 40.0 38.0 41.0 33.0 41.0 18-19 38.19106585158188 40.0 38.0 41.0 33.0 41.0 20-21 38.08198493685403 40.0 38.0 41.0 33.0 41.0 22-23 37.936556479504674 40.0 38.0 41.0 33.0 41.0 24-25 37.88229761290522 40.0 38.0 41.0 33.0 41.0 26-27 37.897343803271156 40.0 38.0 41.0 33.0 41.0 28-29 37.806529733395806 40.0 38.0 41.0 33.0 41.0 30-31 37.620600702655096 40.0 37.5 41.0 32.0 41.0 32-33 37.402570689400555 40.0 37.0 41.0 32.0 41.0 34-35 37.200235737687585 40.0 37.0 41.0 31.0 41.0 36-37 36.984863562027456 40.0 36.0 41.0 30.5 41.0 38-39 36.74729083977677 39.5 36.0 41.0 30.0 41.0 40-41 36.527182214475715 39.0 35.5 41.0 30.0 41.0 42-43 36.24602755560255 39.0 35.0 41.0 30.0 41.0 44-45 36.02170555214215 39.0 35.0 41.0 29.0 41.0 46-47 35.88872470104341 39.0 35.0 41.0 28.0 41.0 48-49 35.63513567223528 39.0 35.0 41.0 27.0 41.0 50-51 34.988375196675975 38.5 34.5 40.0 26.0 40.5 52-53 35.060570911829004 38.5 35.0 40.0 26.0 41.0 54-55 35.1962590088078 39.0 35.0 40.0 26.0 41.0 56-57 34.97869390746612 38.5 35.0 40.0 26.0 41.0 58-59 34.70875492943367 38.0 34.0 40.0 25.5 41.0 60-61 34.33515518030378 38.0 34.0 40.0 24.0 41.0 62-63 33.91415793513476 37.0 34.0 40.0 23.0 41.0 64-65 33.480801325819385 36.5 33.0 40.0 22.0 41.0 66-67 33.0489050868656 36.0 33.0 39.0 20.5 41.0 68-69 32.55433270555032 35.5 32.0 39.0 18.5 40.5 70-71 32.073176223493185 35.0 32.0 38.5 17.5 40.0 72-73 31.585003035601314 35.0 31.0 37.5 12.0 40.0 74-75 31.06326393687408 35.0 31.0 37.0 7.5 39.0 76-77 30.529611060163248 35.0 30.5 36.0 6.5 39.0 78-79 29.967726361964008 34.0 30.0 36.0 2.0 38.5 80-81 29.444541296028557 34.0 29.0 35.0 2.0 37.0 82-83 28.911851972046943 34.0 29.0 35.0 2.0 37.0 84-85 28.418935224096657 34.0 28.5 35.0 2.0 36.0 86-87 27.927010835182344 34.0 27.0 35.0 2.0 36.0 88-89 27.36977069815184 33.0 26.0 35.0 2.0 35.5 90-91 26.758683369462762 33.0 25.0 35.0 2.0 35.0 92-93 26.14671088495527 32.0 24.0 35.0 2.0 35.0 94-95 25.54112610781216 32.0 21.5 34.5 2.0 35.0 96-97 24.69595016144294 32.0 14.5 34.0 2.0 35.0 98-99 23.60771936093755 31.0 2.0 34.0 2.0 35.0 100-101 20.737311459987495 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36479368894129394 1101 2 0.3243116715994958 1101 3 0.30454596344642937 1101 4 0.20300649057082865 1101 5 0.1761000347081989 1101 6 0.23734107005648752 1101 7 0.25402924147999784 1101 8 0.2006480166463831 1101 9 0.19536056402471047 1101 10-11 0.2432128810399803 1101 12-13 0.27070870287411 1101 14-15 0.28519872197681906 1101 16-17 0.46034900269415147 1101 18-19 0.4061006830032525 1101 20-21 0.41660990633755546 1101 22-23 0.4158217011356342 1101 24-25 0.41573954745886965 1101 26-27 0.3912155759121916 1101 28-29 0.41943191907122923 1101 30-31 0.4288599219079714 1101 32-33 0.4095674067210169 1101 34-35 0.4139519300822201 1101 36-37 0.4044469233514931 1101 38-39 0.33050589205714687 1101 40-41 0.3923521525732667 1101 42-43 0.37353489063048784 1101 44-45 0.3215679395397828 1101 46-47 0.3549315231121639 1101 48-49 0.28226236480379185 1101 50-51 0.4328988037054984 1101 52-53 0.3184615622514002 1101 54-55 0.3000886151791349 1101 56-57 0.2357497490176641 1101 58-59 0.3218230445996326 1101 60-61 0.29015834020119513 1101 62-63 0.28071168924847 1101 64-65 0.2752806554082241 1101 66-67 0.21215688762899987 1101 68-69 0.33704895945588476 1101 70-71 0.38127306604793887 1101 72-73 0.417614908131295 1101 74-75 0.47866142861831307 1101 76-77 0.46774195805055996 1101 78-79 0.3932190091660246 1101 80-81 0.5892995008365887 1101 82-83 0.4961706432477051 1101 84-85 0.581401630905237 1101 86-87 0.5794683359076842 1101 88-89 0.6493605397752127 1101 90-91 0.685129782044406 1101 92-93 0.6679675168207773 1101 94-95 0.6976936139239491 1101 96-97 0.9820395033232892 1101 98-99 1.0414444144216652 1101 100-101 1.0380840889094394 1102 1 0.3921140867607811 1102 2 0.3348464144717269 1102 3 0.19000181775196268 1102 4 0.15704296346380175 1102 5 0.21353222528654925 1102 6 0.25574808641666635 1102 7 0.12105928515220654 1102 8 0.1736033734564515 1102 9 0.24032704704448093 1102 10-11 0.35165228356530065 1102 12-13 0.2819682887430943 1102 14-15 0.35683072505465674 1102 16-17 0.23522148591986536 1102 18-19 0.3558461390534049 1102 20-21 0.3931629450398617 1102 22-23 0.42390394929710595 1102 24-25 0.4258104350350038 1102 26-27 0.3730235881477384 1102 28-29 0.3869836064810883 1102 30-31 0.25306958323832873 1102 32-33 0.3842298836861815 1102 34-35 0.4457695289764132 1102 36-37 0.577264134357165 1102 38-39 0.37141920844720744 1102 40-41 0.42577516143848015 1102 42-43 0.3951238248151583 1102 44-45 0.3690649371941319 1102 46-47 0.46881218358398513 1102 48-49 0.32282174870541525 1102 50-51 0.38282122599986224 1102 52-53 0.49739441394594763 1102 54-55 0.48839080687567105 1102 56-57 0.3538512241943792 1102 58-59 0.5287255651801459 1102 60-61 0.44069138685079423 1102 62-63 0.43574572741442097 1102 64-65 0.27240197291520474 1102 66-67 0.3312600659774887 1102 68-69 0.26057545685406325 1102 70-71 0.18713059345441252 1102 72-73 0.43431638368705805 1102 74-75 0.29708616529317666 1102 76-77 0.27195474965397537 1102 78-79 0.3273059928525974 1102 80-81 0.3831648605589777 1102 82-83 0.508473785081609 1102 84-85 0.5511790972350603 1102 86-87 0.47306858582413724 1102 88-89 0.642467305776723 1102 90-91 0.7258523918392861 1102 92-93 0.8805444864763992 1102 94-95 0.917574843593755 1102 96-97 0.940086658312552 1102 98-99 0.9868327578028264 1102 100-101 1.1161014068055657 1103 1 0.46864363979802093 1103 2 0.4260324954636445 1103 3 0.41535907147387263 1103 4 0.2786118180878887 1103 5 0.26520062249788623 1103 6 0.16227168124447644 1103 7 0.26515074768562386 1103 8 0.33057284828630884 1103 9 0.43805816574568723 1103 10-11 0.38785883159045653 1103 12-13 0.3438773299809128 1103 14-15 0.43436031403222586 1103 16-17 0.5053696434925925 1103 18-19 0.4730378555499968 1103 20-21 0.5226018224398032 1103 22-23 0.5250392088401412 1103 24-25 0.5730621445892012 1103 26-27 0.4446439496650072 1103 28-29 0.4595603296114845 1103 30-31 0.5200601476122344 1103 32-33 0.6152122542710359 1103 34-35 0.7245053786081357 1103 36-37 0.7685523040889493 1103 38-39 0.7200938821749574 1103 40-41 0.7341654243078892 1103 42-43 0.6277682461856742 1103 44-45 0.6578956981146504 1103 46-47 0.7550922424776019 1103 48-49 0.8221377450495098 1103 50-51 0.7473633287337123 1103 52-53 0.7598506581084834 1103 54-55 0.8380070566370179 1103 56-57 0.7747595197840482 1103 58-59 0.6622567876988725 1103 60-61 0.551699773989732 1103 62-63 0.5959162422998645 1103 64-65 0.584077364084763 1103 66-67 0.6986436538022431 1103 68-69 0.8391548219779565 1103 70-71 0.8972016810042973 1103 72-73 0.8687428692548913 1103 74-75 0.8166023720322748 1103 76-77 0.8591793568617838 1103 78-79 0.9373285230428543 1103 80-81 0.9162417184277061 1103 82-83 0.9910126222496132 1103 84-85 1.1160046129901922 1103 86-87 1.2266751677878815 1103 88-89 1.1568518548916096 1103 90-91 1.193253828512347 1103 92-93 1.328414400066439 1103 94-95 1.4212710110047304 1103 96-97 1.417128596130322 1103 98-99 1.4920576783132766 1103 100-101 1.667644391973127 1104 1 0.36721499535822844 1104 2 0.2808248644133897 1104 3 0.28499244101459453 1104 4 0.3312840029629882 1104 5 0.3028059244697161 1104 6 0.29999006833823216 1104 7 0.24766898275151306 1104 8 0.23018363679410214 1104 9 0.18383518146459465 1104 10-11 0.3303999284225654 1104 12-13 0.32151138191944284 1104 14-15 0.43918287980479676 1104 16-17 0.474158725703532 1104 18-19 0.4578533526026618 1104 20-21 0.3819872037223817 1104 22-23 0.2888223128719716 1104 24-25 0.45748605471423787 1104 26-27 0.5126328700584111 1104 28-29 0.5398681860401666 1104 30-31 0.5071695954386755 1104 32-33 0.4563850283261033 1104 34-35 0.6104092569743216 1104 36-37 0.5398270994955752 1104 38-39 0.6177478157880216 1104 40-41 0.5982495607903289 1104 42-43 0.6983655386212106 1104 44-45 0.6312515908860519 1104 46-47 0.5216935486791456 1104 48-49 0.7062907835553105 1104 50-51 0.8367423971167369 1104 52-53 0.7716520371567199 1104 54-55 0.7938713948011227 1104 56-57 0.7058066045129507 1104 58-59 0.8302991913803695 1104 60-61 0.8659810356194626 1104 62-63 0.66472850300174 1104 64-65 0.7390429238705138 1104 66-67 0.8758577575540585 1104 68-69 0.8787929927775195 1104 70-71 0.9624293376948643 1104 72-73 0.9347097902371111 1104 74-75 0.9089986032960233 1104 76-77 0.9476423709725914 1104 78-79 0.9104917895976605 1104 80-81 1.0880310475399604 1104 82-83 1.1509966132546303 1104 84-85 1.2100509080667798 1104 86-87 1.137186109547244 1104 88-89 1.0122011554698496 1104 90-91 1.0522444556200945 1104 92-93 1.1374882034048603 1104 94-95 1.124116350724389 1104 96-97 1.0472278821320238 1104 98-99 1.1339198325164652 1104 100-101 1.0365117915695912 1105 1 0.33355453002843305 1105 2 0.3068533143186798 1105 3 0.20788229992270857 1105 4 0.1660937215177043 1105 5 0.1551893574994665 1105 6 0.2104888298671881 1105 7 0.15544330758021374 1105 8 0.23546764684557075 1105 9 0.21349980630067478 1105 10-11 0.277317323102622 1105 12-13 0.0503317307671054 1105 14-15 0.20742832261058197 1105 16-17 0.20384111426609053 1105 18-19 0.22023137667749637 1105 20-21 0.2599254538156899 1105 22-23 0.2776790673792675 1105 24-25 0.3203690476426786 1105 26-27 0.20803485919659437 1105 28-29 0.23505900638502197 1105 30-31 0.31679673681598786 1105 32-33 0.3297071046462534 1105 34-35 0.3158397678710756 1105 36-37 0.3461348191320823 1105 38-39 0.4258873947677415 1105 40-41 0.5259933080584105 1105 42-43 0.4179024442598944 1105 44-45 0.3533760128164687 1105 46-47 0.3426264037890121 1105 48-49 0.46976916070916985 1105 50-51 0.3601308379206145 1105 52-53 0.37781764698144826 1105 54-55 0.44269130454541994 1105 56-57 0.4973558609313429 1105 58-59 0.3631525049141331 1105 60-61 0.34152697081978545 1105 62-63 0.3646139369456236 1105 64-65 0.26895204943610906 1105 66-67 0.39843006490244903 1105 68-69 0.40125732062006847 1105 70-71 0.49868430505151906 1105 72-73 0.5985322647441684 1105 74-75 0.43979791110947275 1105 76-77 0.5508655891610523 1105 78-79 0.4406460146700617 1105 80-81 0.555110271764093 1105 82-83 0.6657291294565475 1105 84-85 0.6491360961127874 1105 86-87 0.6152251538188409 1105 88-89 0.6820792643714704 1105 90-91 0.7316992090136658 1105 92-93 0.8220637002403244 1105 94-95 0.9494611965492119 1105 96-97 0.8139689967759196 1105 98-99 0.7063680931682157 1105 100-101 0.5712021538498142 1106 1 -0.01039768221036752 1106 2 -0.004727847872793234 1106 3 -0.06904286295040407 1106 4 -0.0619046494004607 1106 5 -0.0031961218864395846 1106 6 0.01884480032075686 1106 7 0.054209876300440385 1106 8 -0.08216836305697939 1106 9 -0.05389436045878426 1106 10-11 -0.5934244971450511 1106 12-13 -0.36872986605198577 1106 14-15 -0.2103219857759342 1106 16-17 -0.09513921723650043 1106 18-19 0.001960239142910325 1106 20-21 -0.018811061329266465 1106 22-23 -0.2232098357953376 1106 24-25 -0.15515760739899065 1106 26-27 -0.09712774350271758 1106 28-29 -0.288371927726061 1106 30-31 -0.09428233347470893 1106 32-33 -0.09050704334024573 1106 34-35 -0.2793325019213029 1106 36-37 -0.2978241915769857 1106 38-39 -0.19456861549450366 1106 40-41 0.05609528893876359 1106 42-43 -0.09223518458419022 1106 44-45 -0.015170909192072202 1106 46-47 0.11806189810476297 1106 48-49 0.061073891742928765 1106 50-51 0.044791065555848775 1106 52-53 0.14452724732706912 1106 54-55 -0.2493229418849694 1106 56-57 -0.13689249802890657 1106 58-59 0.07335444499090471 1106 60-61 -0.17898361853105627 1106 62-63 -0.036904991819547206 1106 64-65 -0.023294416411651753 1106 66-67 -0.08852254822925687 1106 68-69 -0.5814285827837438 1106 70-71 -0.19543593654545077 1106 72-73 0.053560001354846065 1106 74-75 0.07114320847494682 1106 76-77 0.055949327054381826 1106 78-79 0.18967965612843685 1106 80-81 0.2739143813054383 1106 82-83 0.32895753037853837 1106 84-85 0.24364146530990283 1106 86-87 0.1813796903181455 1106 88-89 -0.10630068312596919 1106 90-91 -0.2631814663537675 1106 92-93 -0.1506648039342302 1106 94-95 0.042047496623997205 1106 96-97 0.12110366605330114 1106 98-99 -0.17969375454375225 1106 100-101 -0.3805435032606006 1107 1 0.15812623960265526 1107 2 0.1008275989741918 1107 3 0.09066877300568166 1107 4 -0.06616938914414305 1107 5 0.10157883845236881 1107 6 0.10688963485257119 1107 7 -0.013221598227111997 1107 8 0.1332195770112108 1107 9 0.20745909076961055 1107 10-11 0.1000199207580934 1107 12-13 0.10122171104887201 1107 14-15 0.11416761825945798 1107 16-17 0.11434117097861218 1107 18-19 0.2563395228502472 1107 20-21 0.20036325975392089 1107 22-23 0.255107296474236 1107 24-25 0.3110763212183727 1107 26-27 0.33978840687002787 1107 28-29 0.37806749700787634 1107 30-31 0.38020374471103224 1107 32-33 0.2690344903686608 1107 34-35 0.37428378299983933 1107 36-37 0.3755423893493912 1107 38-39 0.3820192382795895 1107 40-41 0.35022566612099126 1107 42-43 0.4773059540818778 1107 44-45 0.42307737240234644 1107 46-47 0.4649069272290163 1107 48-49 0.492510330272971 1107 50-51 0.30115054764853255 1107 52-53 0.37582975279705266 1107 54-55 0.5781869554149708 1107 56-57 0.5147594025271189 1107 58-59 0.44986651453420023 1107 60-61 0.37342693657058845 1107 62-63 0.5990570624081428 1107 64-65 0.5449948302638035 1107 66-67 0.5086790347480203 1107 68-69 0.5664886246054976 1107 70-71 0.5890770550105984 1107 72-73 0.5484347820045379 1107 74-75 0.5445228590429387 1107 76-77 0.6831361999161807 1107 78-79 0.607555603077504 1107 80-81 0.7158775423914747 1107 82-83 0.5841504669633295 1107 84-85 0.5931852154429684 1107 86-87 0.6701171543227886 1107 88-89 0.79122649637522 1107 90-91 0.8445225951173576 1107 92-93 0.8461107358007389 1107 94-95 0.7759160576256896 1107 96-97 0.845680351806319 1107 98-99 0.823458404266912 1107 100-101 0.8849495292789911 1108 1 0.25078094115336214 1108 2 0.17715691951367063 1108 3 0.1919617309788464 1108 4 0.08782472798716157 1108 5 0.2171617144761342 1108 6 0.13950628632562712 1108 7 0.16896356680668845 1108 8 0.102960516997058 1108 9 0.11159295305667172 1108 10-11 0.16681270037150853 1108 12-13 0.12216120445917511 1108 14-15 0.16327822171591322 1108 16-17 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-1.205690876482322 2316 84-85 -1.1982519418289321 2316 86-87 -1.2081419760928753 2316 88-89 -1.2858133158652372 2316 90-91 -1.343996211556668 2316 92-93 -1.263287071644836 2316 94-95 -1.355752002750723 2316 96-97 -1.3466158609895515 2316 98-99 -1.5698725167521062 2316 100-101 -1.6628495411069082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8218.0 3 2086.0 4 2133.0 5 2366.0 6 2868.0 7 3322.0 8 4241.0 9 5315.0 10 6544.0 11 8042.0 12 9906.0 13 12399.0 14 14341.0 15 17040.0 16 20881.0 17 27748.0 18 24935.0 19 20383.0 20 20027.0 21 21023.0 22 22520.0 23 24674.0 24 27614.0 25 30995.0 26 34768.0 27 40323.0 28 47333.0 29 55642.0 30 66438.0 31 82016.0 32 102207.0 33 133414.0 34 182753.0 35 266819.0 36 400364.0 37 543976.0 38 399504.0 39 47272.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5210470691533 18.423166507624497 14.766405896140334 23.289380527081878 2 15.387250566943061 19.42170348513106 40.66313367014275 24.52791227778313 3 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21.957150877671864 22-23 22.725678015129215 25.628938132516282 29.203549671667595 22.441834180686907 24-25 22.486391868918133 25.84234641626392 29.262427251153873 22.408834463664082 26-27 22.05730439043934 26.210580973047847 29.10263843799158 22.629476198521232 28-29 22.26203414711308 26.575952887850484 28.917128819683967 22.24488414535247 30-31 22.372481379178726 26.194514076069176 28.808845818340917 22.624158726411178 32-33 22.101831106180807 26.161592018741654 28.83625502660176 22.90032184847578 34-35 22.71599180687701 26.266947215078968 28.719191401672855 22.29786957637117 36-37 22.708830431357324 25.96636950558824 28.739214059972507 22.585586003081932 38-39 22.22356183352944 26.366402013381073 28.624962357631595 22.78507379545789 40-41 22.80317777411348 26.142908632974898 28.638800888965417 22.415112703946207 42-43 22.54450108984122 26.05805798502494 28.546343331904534 22.851097593229305 44-45 22.46039156187086 26.396325095556268 28.609163919120007 22.534119423452864 46-47 22.783349987042385 26.546674854005726 28.020377647397176 22.64959751155471 48-49 22.601283983975915 26.64651558584094 27.930517799128474 22.82168263105467 50-51 22.67594691546925 26.88448442825801 27.893688689172457 22.545879967100284 52-53 23.032440820354992 26.596851496809204 27.79805198606493 22.572655696770873 54-55 22.655868596571246 26.80584792527477 27.86366755667829 22.6746159214757 56-57 22.63400452233292 26.862252671810992 27.829562868506397 22.674179937349695 58-59 22.704923563405995 26.599734117728584 27.72517474738449 22.970167571480935 60-61 22.377566390072143 26.80305447311506 27.953178022398085 22.866201114414718 62-63 22.46519236064635 26.744357378490108 28.15943950354778 22.63101075731576 64-65 22.31111762751391 26.78504150012455 28.00019257429155 22.90364829806999 66-67 22.277174101338623 26.77348062268531 27.90375255972385 23.045592716252216 68-69 22.720898638730088 26.500797797168048 28.013366521695527 22.764937042406338 70-71 22.571586424365968 26.61503449430634 27.62658050644423 23.18679857488346 72-73 22.480347274153246 26.706841519689206 27.686012366169955 23.126798839987597 74-75 22.405066130755767 27.042982483497628 27.601271639381427 22.950679746365175 76-77 22.534142778727077 26.683887745072116 27.61913272144708 23.16283675475373 78-79 22.67162529813787 26.897451513847454 27.49706420813853 22.933858979876153 80-81 22.67779551865065 26.894679026762784 27.286098684800773 23.141426769785795 82-83 22.68932434539435 26.8868973100921 27.40848468491305 23.015293659600502 84-85 22.654156913747112 27.002615691082944 27.320897008440088 23.022330386729852 86-87 22.783625402575645 26.964540887353944 27.170690250280206 23.08114345979021 88-89 22.732039977513836 26.99191716452512 27.016066810616067 23.25997604734498 90-91 22.649557291918445 26.95536471495189 27.080031135593615 23.315046857536053 92-93 22.759371482838485 26.61104805690819 27.274141577771182 23.355438882482144 94-95 22.61853358047839 26.59602200079864 27.323739041238454 23.461705377484517 96-97 22.861359514863913 26.259635943643023 27.337387807902395 23.54161673359067 98-99 22.73785520631855 26.467155641033358 27.021861586148223 23.773127566499873 100-101 23.105626294198157 25.92056321750492 27.211063013136215 23.762747475160708 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 918.0 1 632.0 2 449.5 3 574.5 4 507.5 5 505.5 6 921.0 7 2060.0 8 3471.5 9 4093.0 10 3549.0 11 2875.5 12 2816.5 13 2917.5 14 3055.0 15 3223.5 16 3442.5 17 3696.5 18 3939.0 19 4234.5 20 4694.5 21 5532.0 22 6676.5 23 8233.5 24 10379.5 25 13147.0 26 16615.0 27 20890.0 28 24212.0 29 27061.5 30 30307.5 31 34497.5 32 41078.5 33 45212.5 34 47212.0 35 50894.5 36 55612.5 37 60626.5 38 65066.0 39 68431.0 40 72432.5 41 77824.5 42 81887.0 43 85483.0 44 89369.0 45 93169.5 46 95684.0 47 99379.0 48 103246.5 49 103018.5 50 101607.5 51 100219.0 52 99297.5 53 99677.5 54 100556.5 55 95981.5 56 88074.0 57 80613.5 58 74988.0 59 67756.5 60 55480.0 61 45978.0 62 38382.5 63 28471.5 64 20442.0 65 15933.0 66 11798.0 67 8828.5 68 6831.5 69 5016.5 70 3588.0 71 2370.5 72 1564.0 73 1105.5 74 804.0 75 547.5 76 392.0 77 285.5 78 176.5 79 112.0 80 68.0 81 51.5 82 42.0 83 26.5 84 22.5 85 17.0 86 14.0 87 11.0 88 6.0 89 4.0 90 2.5 91 2.5 92 2.0 93 1.0 94 1.5 95 1.5 96 2.5 97 2.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4965988503001872 2 0.05247123471488137 3 0.061186054101161194 4 0.06928099093695247 5 0.08430402686643901 6 0.11201642324122 7 0.0752245706857542 8 0.055242474352359475 9 0.054294418686906445 10-11 0.07945435750085234 12-13 0.11429540320625133 14-15 0.06005568003850564 16-17 0.06423077133444305 18-19 0.06350149774563302 20-21 0.07896209782840558 22-23 0.06795006663737417 24-25 0.09294591889383781 26-27 0.07044782867904852 28-29 0.07039313315988777 30-31 0.07444060157778341 32-33 0.07420358766142016 34-35 0.07686543626057675 36-37 0.07168759378002557 38-39 0.04359232877111931 40-41 0.047074610157687186 42-43 0.0448503257118166 44-45 0.0224251628559083 46-47 0.03002784001925282 48-49 0.030337781294497084 50-51 0.02880630675799603 52-53 0.026964890946250715 54-55 0.026636717831286204 56-57 0.026563790472405202 58-59 0.023336754841920833 60-61 0.03050186785197934 62-63 0.02187820766430078 64-65 0.023920173712968854 66-67 0.0167732925426306 68-69 0.01932575010346569 70-71 0.04395696556552432 72-73 0.040292365781753936 74-75 0.03480458202595849 76-77 0.02406602843073086 78-79 0.015861700556618067 80-81 0.01870586755297717 82-83 0.023063277246117075 84-85 0.019726850577311203 86-87 0.0097904979297746 88-89 0.04426690684076858 90-91 0.05617229817809226 92-93 0.035971419768054534 94-95 0.05668278969025928 96-97 0.04689229176048467 98-99 0.036554838639102555 100-101 0.1734212594190242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2742455.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.512305401044934 #Duplication Level Percentage of deduplicated Percentage of total 1 72.61964006837219 37.40805077315949 2 14.563390579502599 15.003876464120774 3 5.345058082650234 8.260087930194077 4 2.568899207706296 5.293196821274764 5 1.330037890423645 3.425665900323217 6 0.8532035275725259 2.637028840893889 7 0.550691899022397 1.985718649902618 8 0.39366747497188703 1.6222975357768048 9 0.2813941804279749 1.3045736664254328 >10 1.3599473018023853 12.271820814268429 >50 0.08713201599658471 3.0827690269475374 >100 0.04168787313431267 3.9995972478724986 >500 0.0027668383549507907 0.9677530496560856 >1k 0.0022702263424992197 1.755119065080316 >5k 1.4188914640620123E-4 0.55694862265906 >10k+ 7.094457320310062E-5 0.4254955914448449 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11643 0.4245466197257567 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9285 0.33856526360505457 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5955 0.21714121106818526 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.6463679440501304E-5 0.0 0.0 0.0 0.0 3 3.6463679440501304E-5 0.0 0.0 3.6463679440501304E-5 0.0 4 3.6463679440501304E-5 0.0 0.0 7.292735888100261E-5 0.0 5 1.0939103832150391E-4 0.0 0.0 7.292735888100261E-5 3.6463679440501304E-5 6 1.4585471776200522E-4 0.0 0.0 1.4585471776200522E-4 3.6463679440501304E-5 7 1.4585471776200522E-4 0.0 0.0 2.1878207664300782E-4 3.6463679440501304E-5 8 1.4585471776200522E-4 0.0 0.0 2.1878207664300782E-4 3.6463679440501304E-5 9 1.4585471776200522E-4 0.0 0.0 2.9170943552401043E-4 3.6463679440501304E-5 10-11 1.4585471776200522E-4 0.0 0.0 4.7402783272651694E-4 3.6463679440501304E-5 12-13 1.6408655748225586E-4 0.0 0.0 6.928099093695247E-4 3.281731149645117E-4 14-15 2.5524575608350914E-4 0.0 0.0 7.657372682505273E-4 4.557959930062663E-4 16-17 2.5524575608350914E-4 0.0 0.0 7.657372682505273E-4 5.469551916075196E-4 18-19 3.8286863412526366E-4 0.0 0.0 9.662875051732846E-4 6.016507107682715E-4 20-21 4.7402783272651694E-4 0.0 0.0 0.0011303740626555404 6.563462299290234E-4 22-23 4.7402783272651694E-4 0.0 0.0 0.0011850695818162924 6.563462299290234E-4 24-25 5.834188710480209E-4 0.0 0.0 0.001458547177620052 6.928099093695247E-4 26-27 5.834188710480209E-4 0.0 0.0 0.0021148934075490757 7.110417490897754E-4 28-29 6.928099093695247E-4 0.0 0.0 0.004703814647824668 7.29273588810026E-4 30-31 7.29273588810026E-4 0.0 0.0 0.013637416110747487 7.29273588810026E-4 32-33 7.657372682505273E-4 0.0 0.0 0.03099412752442611 7.657372682505273E-4 34-35 8.386646271315299E-4 0.0 0.0 0.056536934972497274 8.022009476910286E-4 36-37 9.115919860125326E-4 0.0 0.0 0.09836077529075227 8.022009476910286E-4 38-39 9.845193448935353E-4 0.0 0.0 0.16054958057652724 8.022009476910286E-4 40-41 0.001002751184613786 0.0 0.0 0.22507206134649427 8.022009476910286E-4 42-43 0.0010939103832150391 0.0 0.0 0.29442597964232775 8.022009476910286E-4 44-45 0.001148605902375791 0.0 0.0 0.38084489991631587 8.751283065720313E-4 46-47 0.0013491561392985482 0.0 0.0 0.48039074478888444 9.115919860125326E-4 48-49 0.0013856198187390495 0.0 0.0 0.581686846274597 9.845193448935353E-4 50-51 0.0013856198187390495 0.0 0.0 0.6858088829169484 0.0010574467037745379 52-53 0.0014038516584593001 0.0 0.0 0.7856646690647613 0.001203301421536543 54-55 0.001458547177620052 0.0 0.0 0.8986656116508749 0.0012397651009770443 56-57 0.001567938215941556 0.0 0.0 1.0271089224800407 0.001257996940697295 58-59 0.001567938215941556 0.0 0.0 1.1620792319290563 0.0012762287804175456 60-61 0.001567938215941556 3.6463679440501304E-5 0.0 1.3046704503811366 0.0012762287804175456 62-63 0.001567938215941556 3.6463679440501304E-5 0.0 1.4456937306172755 0.001312692459858047 64-65 0.0016044018953820572 3.6463679440501304E-5 0.0 1.593262241312984 0.001312692459858047 66-67 0.0017502566131440626 3.6463679440501304E-5 0.0 1.7609769348995699 0.001312692459858047 68-69 0.0017502566131440626 3.6463679440501304E-5 0.0 1.9447903429591369 0.001312692459858047 70-71 0.0018231839720250652 3.6463679440501304E-5 0.0 2.1359329505862448 0.0013491561392985482 72-73 0.0019508068500668197 3.6463679440501304E-5 0.0 2.331031867432647 0.0013856198187390495 74-75 0.002005502369227572 3.6463679440501304E-5 0.0 2.5263495663556923 0.0014220834981795508 76-77 0.002005502369227572 3.6463679440501304E-5 0.0 2.7380759210269634 0.0014220834981795508 78-79 0.0020784297281085744 3.6463679440501304E-5 0.0 2.9731207987004344 0.0014220834981795508 80-81 0.0023701391636325848 5.4695519160751956E-5 0.0 3.2124501587081644 0.0014403153378998014 82-83 0.0023701391636325848 7.292735888100261E-5 0.0 3.462554535990563 0.0014767790173403027 84-85 0.0024795302019540886 7.292735888100261E-5 0.0 3.7196417078858177 0.0014950108570605533 86-87 0.002552457560835091 7.292735888100261E-5 0.0 3.986154740916442 0.001567938215941556 88-89 0.002552457560835091 7.292735888100261E-5 0.0 4.271391873339763 0.0016408655748225587 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5595 0.0 56.41806 1 GTATCAA 9605 0.0 40.719585 1 ATCAACG 12370 0.0 31.309395 3 TATCAAC 12465 0.0 31.184578 2 TCAACGC 12515 0.0 30.832727 4 CAACGCA 12835 0.0 30.026985 5 AACGCAG 13310 0.0 29.026806 6 TGGTATC 2440 0.0 28.434256 2 ACGCAGA 14915 0.0 25.820852 7 CGCAGAG 14930 0.0 25.603836 8 GTGGTAT 3095 0.0 23.607367 1 GCAGAGT 16555 0.0 22.773876 9 TAAGGTG 1485 0.0 22.400534 5 GAGTACT 10305 0.0 22.142302 12-13 TACATGG 9175 0.0 21.701353 2 CAGAGTA 15620 0.0 21.519064 10-11 GTACATG 9610 0.0 21.417595 1 AGAGTAC 14735 0.0 21.004671 10-11 ACATGGG 9095 0.0 21.00439 3 GTACTTT 10895 0.0 20.843208 14-15 >>END_MODULE