FastQCFastQC Report
Thu 26 May 2016
SRR938137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742455
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111170.405366724340053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94890.34600385421091684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61240.22330357289362998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52850.053.664911
GTATCAA95000.044.1568571
TATCAAC121950.034.203752
ATCAACG123200.033.774673
TCAACGC124100.033.489014
CAACGCA126450.032.9041985
AACGCAG131950.031.532676
ACGCAGA149600.027.6219087
CGCAGAG150450.027.2132938
GCAGAGT166300.024.3625559
TGGTATC24500.023.4628262
GTAAGGT14250.023.3318524
GTGGTAT27800.022.7283481
TAAGGTG15100.022.6475685
GAGTACT101650.022.2649412-13
AGAGTAC148350.021.61136610-11
AGTACTT105750.021.17714112-13
CAGAGTA158500.021.05150210-11
TACATGG91850.021.0511722
GTACTTT106350.020.96834414-15
Produced by FastQC (version 0.11.5)