FastQCFastQC Report
Thu 26 May 2016
SRR938136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760896
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110560.40044970907995087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89230.32319218108903774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56770.20562165326039084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51750.053.2796821
GTATCAA91300.043.082681
ATCAACG115750.033.4097823
TATCAAC116900.033.242672
TCAACGC117950.032.7866254
CAACGCA120750.031.9869615
AACGCAG125250.030.9443556
ACGCAGA142300.027.066647
CGCAGAG143300.026.7108768
TGGTATC25600.025.2689532
TACATGG90100.024.7063942
GTGGTAT28500.024.3950331
GTACATG95100.024.3860761
GCAGAGT156650.023.7359689
ACATGGG90000.023.4134453
GTAAGGT15650.023.0995394
TAAGGTG16900.022.7982965
GAGTACT99650.021.94582712-13
AGAGTAC141600.020.98475810-11
CAGAGTA149100.020.88578810-11
Produced by FastQC (version 0.11.5)