FastQCFastQC Report
Thu 26 May 2016
SRR938136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760896
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107200.3882797468647859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90250.3268866339043557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59090.21402472240895706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55800.052.0148431
GTATCAA94050.041.3661771
TATCAAC122600.031.5770592
ATCAACG122750.031.5720233
TCAACGC124150.031.1366654
CAACGCA126850.030.473925
TGGTATC23950.030.3452322
AACGCAG132350.029.2064746
ACGCAGA147250.025.9622827
CGCAGAG148400.025.6010868
GTGGTAT29700.025.2709121
GCGTAAT1000.001653640123.7514571
TAAGGTG15750.022.91545
GCAGAGT166800.022.2645029
TACATGG91000.022.1846262
GTACATG96750.021.6524931
GTAAGGT16150.021.4656854
GAGTACT103850.021.3560312-13
ACATGGG91000.021.0328143
AGAGTAC148150.020.62799510-11
Produced by FastQC (version 0.11.5)