##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938136_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2760896 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51393424453511 34.0 31.0 34.0 31.0 34.0 2 32.729031444864276 34.0 31.0 34.0 31.0 34.0 3 32.78784930689167 34.0 31.0 34.0 31.0 34.0 4 36.245970511022506 37.0 35.0 37.0 35.0 37.0 5 36.21597807378474 37.0 37.0 37.0 35.0 37.0 6 36.24445650977074 37.0 37.0 37.0 35.0 37.0 7 36.20065297642505 37.0 37.0 37.0 35.0 37.0 8 36.18516343969494 37.0 37.0 37.0 35.0 37.0 9 37.87792187753541 39.0 38.0 39.0 35.0 39.0 10-11 37.856106495862214 39.0 38.0 39.0 35.0 39.0 12-13 37.777738458819165 39.0 38.0 39.0 35.0 39.0 14-15 39.05943921828276 40.0 38.0 41.0 36.0 41.0 16-17 38.94524494946568 40.0 38.0 41.0 36.0 41.0 18-19 39.18705394915274 40.5 39.0 41.0 36.0 41.0 20-21 39.163643614246965 40.0 39.0 41.0 36.0 41.0 22-23 39.0732298500197 40.0 39.0 41.0 35.5 41.0 24-25 38.98163494749531 40.0 38.5 41.0 35.0 41.0 26-27 38.81370757898885 40.0 38.0 41.0 35.0 41.0 28-29 38.69878456124388 40.0 38.0 41.0 34.5 41.0 30-31 38.464084123415006 40.0 38.0 41.0 34.0 41.0 32-33 38.26390381962957 40.0 38.0 41.0 34.0 41.0 34-35 38.11651235685807 40.0 38.0 41.0 34.0 41.0 36-37 38.12751440112196 40.0 38.0 41.0 34.0 41.0 38-39 37.98948928174042 40.0 38.0 41.0 33.0 41.0 40-41 37.822760437191405 40.0 37.0 41.0 33.0 41.0 42-43 37.60287475515195 40.0 37.0 41.0 33.0 41.0 44-45 37.33159362033195 40.0 37.0 41.0 32.0 41.0 46-47 36.96160630461995 40.0 36.0 41.0 31.0 41.0 48-49 36.57294751414034 39.0 36.0 41.0 30.5 41.0 50-51 36.19690383122001 39.0 35.0 41.0 30.0 41.0 52-53 35.68335189011104 39.0 35.0 40.0 28.0 41.0 54-55 35.369047222351 38.0 35.0 40.0 27.5 41.0 56-57 35.02755989359976 38.0 34.0 40.0 27.0 41.0 58-59 34.580226129488395 38.0 34.0 40.0 26.0 41.0 60-61 34.10348615087276 37.0 33.0 40.0 25.5 41.0 62-63 33.6401048789958 36.5 33.0 39.0 23.5 41.0 64-65 33.150232750527366 36.0 32.0 39.0 22.5 40.0 66-67 32.6002556778669 35.5 31.5 39.0 21.5 40.0 68-69 32.01884841008136 35.0 31.0 38.0 19.5 40.0 70-71 31.414685305060388 35.0 30.5 37.5 17.0 39.5 72-73 30.836350771633555 34.5 30.0 37.0 12.0 39.0 74-75 30.24396482156517 34.0 29.0 36.0 8.0 39.0 76-77 28.891454621977793 32.5 28.0 35.0 7.0 37.0 78-79 29.58321646306127 34.0 29.0 35.0 6.0 37.0 80-81 29.264542561545237 34.0 29.0 35.0 2.0 37.0 82-83 28.708022323187834 34.0 28.5 35.0 2.0 36.5 84-85 28.12247926035606 33.0 27.0 35.0 2.0 36.0 86-87 27.489026750735995 33.0 26.0 35.0 2.0 35.5 88-89 26.750137455376805 32.0 25.0 35.0 2.0 35.0 90-91 26.209629772363755 32.0 24.5 34.0 2.0 35.0 92-93 25.39463112699645 31.5 21.5 34.0 2.0 35.0 94-95 24.575344924256473 31.0 18.0 34.0 2.0 35.0 96-97 23.525528125651963 31.0 4.5 34.0 2.0 35.0 98-99 22.066559189480515 30.0 2.0 34.0 2.0 35.0 100-101 17.661099874823243 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06124138263572121 1101 2 0.0774050513028044 1101 3 0.059459028947443926 1101 4 0.09318324004720324 1101 5 0.023086958993985718 1101 6 0.041411979563719115 1101 7 -0.032877930831787694 1101 8 0.0399552411283608 1101 9 -0.017174328409254258 1101 10-11 -7.097000814511034E-4 1101 12-13 0.14980799216813523 1101 14-15 0.16328724893791247 1101 16-17 0.10245204505410754 1101 18-19 0.14997755969918103 1101 20-21 0.07109303338059902 1101 22-23 0.16652086236645403 1101 24-25 0.09828705970965501 1101 26-27 0.0947988800592654 1101 28-29 0.19937324292155267 1101 30-31 0.2066989318330954 1101 32-33 0.09097259406998859 1101 34-35 0.12990951020049835 1101 36-37 0.19476329961953098 1101 38-39 0.1795155026420261 1101 40-41 0.25860432227981534 1101 42-43 0.22396164265558838 1101 44-45 0.1952611648909084 1101 46-47 0.22546183114270235 1101 48-49 0.03971554583598902 1101 50-51 0.01182931638895468 1101 52-53 -0.048947649237327084 1101 54-55 -0.3539579232765746 1101 56-57 -0.32738720762729656 1101 58-59 -0.06436952626716419 1101 60-61 -0.06846037214173606 1101 62-63 -0.05725824621991649 1101 64-65 -0.06706255998049215 1101 66-67 -0.38215615756083565 1101 68-69 -0.1847743496917289 1101 70-71 -0.26363640620750317 1101 72-73 -0.29362275233243196 1101 74-75 -0.22070123866695468 1101 76-77 -0.2830881582729745 1101 78-79 -0.429327964038972 1101 80-81 -0.4023099162968826 1101 82-83 -0.14670812557514168 1101 84-85 -0.38108012035634076 1101 86-87 -0.5239904584235333 1101 88-89 -0.4681677764604295 1101 90-91 -0.6278820215520149 1101 92-93 -0.6612578081119231 1101 94-95 -0.7165483922110596 1101 96-97 -0.7160773281040029 1101 98-99 -0.8938970844414733 1101 100-101 -1.0198260398591863 1102 1 0.09044406661063675 1102 2 0.09971334949121768 1102 3 0.07083783963681611 1102 4 0.03397144996620938 1102 5 0.01836374806133989 1102 6 0.028247674170174264 1102 7 0.09583322281879703 1102 8 0.08136476000026249 1102 9 0.19039121727580977 1102 10-11 0.2048376300680701 1102 12-13 0.12701414576887515 1102 14-15 0.20634824655503792 1102 16-17 0.23547447465626448 1102 18-19 0.1849549772207908 1102 20-21 0.21413500455346224 1102 22-23 0.17368419400486346 1102 24-25 0.19944825440418157 1102 26-27 0.26369770590332564 1102 28-29 0.07931438557169201 1102 30-31 0.12518399482807752 1102 32-33 0.0070239338972371 1102 34-35 0.09172709229670062 1102 36-37 0.16984073475514805 1102 38-39 0.1589499935143195 1102 40-41 0.17087114448143126 1102 42-43 0.23342567852168372 1102 44-45 0.11506968740858525 1102 46-47 0.09975610792180589 1102 48-49 0.20732913121570817 1102 50-51 0.26817151986722365 1102 52-53 0.17605808362142028 1102 54-55 0.33802247200819835 1102 56-57 0.3272207645359302 1102 58-59 0.2513689157910832 1102 60-61 0.08004808756374615 1102 62-63 0.10427310836321624 1102 64-65 0.09481401240141452 1102 66-67 0.003750897012658072 1102 68-69 0.038919389361829815 1102 70-71 -0.07072762014449552 1102 72-73 -0.17279635658903558 1102 74-75 -0.07423126721493034 1102 76-77 -0.25345899945783046 1102 78-79 -0.2195704722726859 1102 80-81 -0.3879493543770245 1102 82-83 -0.35236055813512124 1102 84-85 -0.3161716965435417 1102 86-87 -0.6865422618645773 1102 88-89 -0.7765885151185081 1102 90-91 -0.9088676245989014 1102 92-93 -0.6265948669725923 1102 94-95 -0.7478361750736298 1102 96-97 -0.8450942342213743 1102 98-99 -1.0936086136358476 1102 100-101 -1.4171494595011254 1103 1 0.1179456946705244 1103 2 0.11312714725944062 1103 3 0.12172878951145094 1103 4 0.10963486470778605 1103 5 0.0995954422188916 1103 6 0.08979903434735803 1103 7 0.06588258370697275 1103 8 0.08773700212973523 1103 9 0.1881562921665676 1103 10-11 0.11975482709949858 1103 12-13 0.1634524855567676 1103 14-15 0.21095022917018724 1103 16-17 0.21874297397830134 1103 18-19 0.17716891844704463 1103 20-21 0.22222740599684698 1103 22-23 0.17714181568464227 1103 24-25 0.2838851029836036 1103 26-27 0.28567502002922396 1103 28-29 0.19692212853013302 1103 30-31 0.2218719547353345 1103 32-33 0.1784782239530145 1103 34-35 0.25602279516239435 1103 36-37 0.28954482110680857 1103 38-39 0.28774691231267013 1103 40-41 0.3528378250953992 1103 42-43 0.2806129101277506 1103 44-45 0.28565300474969746 1103 46-47 0.18390092817455894 1103 48-49 0.15960471849702174 1103 50-51 0.18902256261205252 1103 52-53 0.14297441235424202 1103 54-55 0.14562260162377783 1103 56-57 0.21125201384774073 1103 58-59 0.41093058229520807 1103 60-61 0.2349284920147241 1103 62-63 0.27678744630125607 1103 64-65 0.2471997886635151 1103 66-67 0.21046778447675507 1103 68-69 0.16055323790664744 1103 70-71 -0.09561986932103039 1103 72-73 -0.10885552358865169 1103 74-75 -0.01881981636975638 1103 76-77 0.03628241429589707 1103 78-79 -0.26460669857144126 1103 80-81 -0.3573688055242137 1103 82-83 -0.40577256267796 1103 84-85 -0.5103630620060216 1103 86-87 -0.5694174313558342 1103 88-89 -0.6937230196062885 1103 90-91 -0.8880460756926141 1103 92-93 -0.6331217354662542 1103 94-95 -0.6245512221222036 1103 96-97 -0.8106235660404302 1103 98-99 -0.9683973591016617 1103 100-101 -1.7337820968323001 1104 1 0.13966475552072666 1104 2 0.14847906619417728 1104 3 0.0983600062175114 1104 4 0.07957153396107941 1104 5 0.0604476120687778 1104 6 0.07161215560076784 1104 7 0.0940160729533801 1104 8 0.11753809375965574 1104 9 0.16863258992239594 1104 10-11 0.13412627569645963 1104 12-13 0.12305961559994927 1104 14-15 0.21403720077326938 1104 16-17 0.14554145752099856 1104 18-19 0.14712167964614054 1104 20-21 0.1922771658692639 1104 22-23 0.15006413688226417 1104 24-25 0.1411838136015504 1104 26-27 0.15134794944280117 1104 28-29 0.09607897016989142 1104 30-31 0.06003570279982995 1104 32-33 0.13952066616206338 1104 34-35 0.025234045458901733 1104 36-37 -4.5886216077661857E-4 1104 38-39 0.09936103579629929 1104 40-41 0.11656678785625729 1104 42-43 0.026884197686058542 1104 44-45 0.03546362638752498 1104 46-47 -0.04320574593016602 1104 48-49 -0.016562007173448023 1104 50-51 -0.08237033833665919 1104 52-53 0.08124704925200632 1104 54-55 0.04269255629217383 1104 56-57 -0.023095737869027744 1104 58-59 0.014096344832914554 1104 60-61 -0.09546657121470759 1104 62-63 -0.007406895532199087 1104 64-65 -0.076829122007112 1104 66-67 -0.3648589118229353 1104 68-69 -0.237965587881952 1104 70-71 -0.3232930395177931 1104 72-73 -0.46009487161452967 1104 74-75 -0.5636641953338852 1104 76-77 -0.581231552917064 1104 78-79 -0.47300574068346535 1104 80-81 -0.5112300083196857 1104 82-83 -0.5952692304531801 1104 84-85 -0.5879274327692201 1104 86-87 -0.5106469632947714 1104 88-89 -0.6261992780262524 1104 90-91 -0.6344989936391876 1104 92-93 -0.5759303640867905 1104 94-95 -0.7332369690686065 1104 96-97 -0.7762481898166982 1104 98-99 -0.8348349539398434 1104 100-101 -1.7723622388943827 1105 1 0.13953125197036798 1105 2 0.13387544657916806 1105 3 0.0153708617249535 1105 4 0.03197918705210867 1105 5 0.054357346980793864 1105 6 0.02705701446248554 1105 7 0.045526749065423644 1105 8 0.08009702306118527 1105 9 0.04430490545803423 1105 10-11 0.1139855963302665 1105 12-13 0.1616471480371402 1105 14-15 0.12594237987662638 1105 16-17 0.07974506712324825 1105 18-19 0.23132904403573917 1105 20-21 0.1418637220617356 1105 22-23 0.13506960218385444 1105 24-25 0.1536129941400688 1105 26-27 0.08223441301718992 1105 28-29 0.12457780618581182 1105 30-31 0.19470031684941347 1105 32-33 0.13089842280178487 1105 34-35 0.1856277140530267 1105 36-37 0.19590274889331027 1105 38-39 0.221642778351395 1105 40-41 0.19798434524192743 1105 42-43 0.12411107862592274 1105 44-45 0.19411715964242404 1105 46-47 0.18825728610234194 1105 48-49 0.28062774309147187 1105 50-51 0.19424234283182784 1105 52-53 0.17770753637308445 1105 54-55 0.2547016178456616 1105 56-57 0.17874285633858733 1105 58-59 0.19342665764842337 1105 60-61 0.1566448708739543 1105 62-63 -0.0031314443319203633 1105 64-65 0.012113446434319997 1105 66-67 -0.07665937407897871 1105 68-69 -0.014381351313659252 1105 70-71 -0.0703058975784856 1105 72-73 0.004344371754097409 1105 74-75 -0.17593650920073145 1105 76-77 -0.08748786304581202 1105 78-79 -0.09977153373627345 1105 80-81 -0.15557655016053573 1105 82-83 -0.17398292374546642 1105 84-85 -0.20183349633359882 1105 86-87 -0.10259377881790144 1105 88-89 -0.3253949191353307 1105 90-91 -0.4245976193870149 1105 92-93 -0.09615156278179526 1105 94-95 -0.3151451520018469 1105 96-97 -0.6790955702267212 1105 98-99 -0.7659771602528913 1105 100-101 -1.512693661458151 1106 1 0.20061841942303715 1106 2 0.18256406118153734 1106 3 0.24556017959393728 1106 4 0.1671207053558419 1106 5 0.17431507576809224 1106 6 0.1465927832795657 1106 7 0.15885948067331412 1106 8 0.1329924506999376 1106 9 0.13754301573926853 1106 10-11 0.20067242079943526 1106 12-13 0.14863521130857293 1106 14-15 0.1723545918945888 1106 16-17 0.23918538724118577 1106 18-19 0.23673780365394492 1106 20-21 0.34091299585433177 1106 22-23 0.3447577579705694 1106 24-25 0.29566532921088395 1106 26-27 0.3060913227057185 1106 28-29 0.30230945167926393 1106 30-31 0.3820422698997774 1106 32-33 0.37815305044655645 1106 34-35 0.3734284827572836 1106 36-37 0.2631624912043904 1106 38-39 0.21154018416255127 1106 40-41 0.2961389285094427 1106 42-43 0.230678911887793 1106 44-45 0.21656302315604847 1106 46-47 0.19635011729578622 1106 48-49 0.04972274801809817 1106 50-51 0.05443933270028367 1106 52-53 0.06783547824640124 1106 54-55 0.0681731223849269 1106 56-57 -0.07044067744453741 1106 58-59 0.05054920655742734 1106 60-61 -0.0740111602954201 1106 62-63 0.10156502278754687 1106 64-65 0.021165277560065476 1106 66-67 -0.06470346551116535 1106 68-69 -0.15851490427677817 1106 70-71 -0.6663920496194642 1106 72-73 -0.46377132500636264 1106 74-75 -0.287247443304274 1106 76-77 -0.3158445508480838 1106 78-79 -0.24210630368911268 1106 80-81 -0.3509624442117243 1106 82-83 -0.399843203096502 1106 84-85 -0.5497312180506206 1106 86-87 -0.48612417723536083 1106 88-89 -0.7198722077604636 1106 90-91 -0.8854256688187583 1106 92-93 -0.6645722591285725 1106 94-95 -0.8522746207534908 1106 96-97 -1.252059293703791 1106 98-99 -1.4626086259808666 1106 100-101 -1.7156594157586085 1107 1 -0.005647512926707066 1107 2 -0.01257763085982333 1107 3 -0.01524875340170695 1107 4 -0.03737540202079259 1107 5 -0.01740141069345924 1107 6 -0.03022514000276999 1107 7 -0.016811382286789467 1107 8 0.006026961667181752 1107 9 -0.03668358961496665 1107 10-11 -0.027029726375204177 1107 12-13 -0.010870006170151214 1107 14-15 0.02936806217554988 1107 16-17 -0.05516696919723785 1107 18-19 -0.05296647265414123 1107 20-21 -0.05228321081759191 1107 22-23 0.027511432653852808 1107 24-25 -0.03202132459284002 1107 26-27 -0.04399991603403919 1107 28-29 -0.020820223015036277 1107 30-31 -0.07817281940400989 1107 32-33 0.044193578350459006 1107 34-35 0.06771253064489002 1107 36-37 -0.019848166857606486 1107 38-39 -0.09496971713434021 1107 40-41 -0.05058914699353778 1107 42-43 -0.038887425217652094 1107 44-45 -0.14696007298410763 1107 46-47 -0.06917706358553488 1107 48-49 -0.1321927697290448 1107 50-51 -0.1345406113032368 1107 52-53 -0.12608960545222914 1107 54-55 -0.0913928979671681 1107 56-57 -0.14134433346691821 1107 58-59 -0.1328025122427121 1107 60-61 -0.27136936840789616 1107 62-63 -0.3000591644990749 1107 64-65 -0.3185887152537603 1107 66-67 -0.33143933590119445 1107 68-69 -0.43497413880785274 1107 70-71 -0.5390671832411797 1107 72-73 -0.776822114341968 1107 74-75 -0.6399384696815673 1107 76-77 -0.7181074781724703 1107 78-79 -0.5246928565449203 1107 80-81 -0.39901151894761355 1107 82-83 -0.4301016876107049 1107 84-85 -0.6873130402638488 1107 86-87 -0.8477080542138644 1107 88-89 -0.7226244501676824 1107 90-91 -0.996320262940646 1107 92-93 -1.203420819346828 1107 94-95 -1.375220991655155 1107 96-97 -1.4311747117424822 1107 98-99 -1.4629590516009792 1107 100-101 -1.8961482350535714 1108 1 -0.04712087005817267 1108 2 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2316 98-99 2.9547705155360298 2316 100-101 3.432989131154063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 6.0 4 13.0 5 41.0 6 97.0 7 243.0 8 587.0 9 1194.0 10 2079.0 11 3414.0 12 5263.0 13 7824.0 14 10483.0 15 14506.0 16 19129.0 17 26530.0 18 27439.0 19 21774.0 20 20908.0 21 22041.0 22 23477.0 23 25915.0 24 29211.0 25 33410.0 26 38844.0 27 44922.0 28 54690.0 29 65854.0 30 80256.0 31 97395.0 32 121882.0 33 157506.0 34 213786.0 35 303859.0 36 434866.0 37 541115.0 38 294654.0 39 15673.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7320064695026 18.807742393534845 15.169824443200774 23.290426693761784 2 15.522744471416042 19.71228577835239 40.23865819337465 24.52631155685692 3 19.299624002955813 23.576970724391995 29.989929944288683 27.13347532836351 4 13.21582312981841 16.695998942371947 36.30569072710117 33.782487200708466 5 13.872981020789513 37.07739210529461 34.249146009780276 14.800480864135599 6 32.75621763684886 35.89564251087344 16.795225828511157 14.552914023766542 7 28.729694997566007 32.75519976123693 20.029331057743573 18.485774183453486 8 26.55076467929252 35.14464144973226 19.146719036138993 19.157874834836228 9 26.130393901110367 16.844241869306195 19.932550882032498 37.09281334755094 10-11 25.245844102784027 26.430405201789565 26.77409797399105 21.54965272143536 12-13 26.054154218165294 23.549205152208113 27.3911943480227 23.005446281603888 14-15 22.690948880363475 25.046361760819675 25.74908652843135 26.513602830385498 16-17 22.34819555902635 28.291606803574382 26.534497893465097 22.82569974393417 18-19 22.388728152020214 26.692747571802776 28.893609176151507 22.0249151000255 20-21 22.8297625118802 25.81714414450961 29.297536017292934 22.055557326317256 22-23 22.69717874197362 25.676247855768565 29.19008901458077 22.436484387677044 24-25 22.512447542113005 25.883317287178357 29.2400968419863 22.364138328722326 26-27 22.045731521761184 26.245051312742547 29.111015067774552 22.59820209772172 28-29 22.26323615440203 26.5535015385507 28.901607011227163 22.281655295820105 30-31 22.382911952918114 26.1821815143181 28.833851841146448 22.601054691617342 32-33 22.166753112033195 26.09201867799439 28.860612641693134 22.880615568279282 34-35 22.67593261719634 26.267466479753825 28.76378527481802 22.292815628231807 36-37 22.71128549269387 25.96700131389862 28.703659266516773 22.61805392689073 38-39 22.240982812659034 26.294610532919492 28.679018592725107 22.785388061696363 40-41 22.812326139366686 26.11109622026983 28.63229272914831 22.44428491121517 42-43 22.514472243934915 26.051469826521423 28.6056370576376 22.82842087190606 44-45 22.493132664178585 26.294163199193303 28.639524994784303 22.57317914184381 46-47 22.82208640065254 26.511888270952866 28.012815416458476 22.653209911936116 48-49 22.595394437785938 26.595931947760114 27.91513137663766 22.893542237816288 50-51 22.735807042902195 26.831371357256167 27.890709852054464 22.542111747787175 52-53 23.04946877045472 26.594114257004907 27.75997212271944 22.596444849820934 54-55 22.6730141251175 26.7868300802156 27.78076817891212 22.759387615754786 56-57 22.65583863368989 26.88791862854576 27.72449194916367 22.731750788600678 58-59 22.740749918756446 26.62613002808835 27.674833047667597 22.958287005487605 60-61 22.444271909507176 26.897248924992034 27.806425942396633 22.852053223104154 62-63 22.488177988252797 26.831429143826597 27.934871248483418 22.745521619437188 64-65 22.292301488939376 26.882802404242746 27.843517721164503 22.981378385653375 66-67 22.270989369538714 26.85972395323256 27.705382754385464 23.163903922843257 68-69 22.713293843095325 26.546508758854596 27.816650465016934 22.92354693303314 70-71 22.602007630364742 26.69546302490003 27.413216510467525 23.289312834267704 72-73 22.60108604368842 26.732655873602667 27.454596198249426 23.211661884459485 74-75 22.498957134657054 27.10051555495017 27.30267656394028 23.09785074645249 76-77 22.53090064913281 26.696664635485007 27.561661820571548 23.21077289481063 78-79 22.670572519927497 26.93412015589115 27.39500407770501 23.000303246476346 80-81 22.76477675479397 26.914526514986857 27.18170134089124 23.13899538932793 82-83 22.72550223830979 26.917817525666738 27.305042401975438 23.051637834048034 84-85 22.717703498355956 26.97520675528608 27.226251219084524 23.08083852727344 86-87 22.82179347826087 26.929221014492754 27.082590579710146 23.16639492753623 88-89 22.696017233271576 27.083458624280045 26.846396076865727 23.374128065582656 90-91 22.625610985732706 27.02403799297776 26.972572261256794 23.37777876003274 92-93 22.758567469998027 26.68970490655818 27.059787656191496 23.491939967252296 94-95 22.58225486555756 26.71089214718481 27.121872141569835 23.584980845687788 96-97 22.752965085529222 26.378864392166523 27.186881896495514 23.68128862580874 98-99 22.582406715120324 26.56911093916632 26.888084225900684 23.960398119812677 100-101 23.107705164445438 26.01834422379366 26.80643476871756 24.067515843043342 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 809.0 1 541.0 2 380.5 3 514.5 4 432.5 5 412.0 6 841.5 7 1944.0 8 3212.0 9 3736.5 10 3188.5 11 2513.5 12 2521.5 13 2732.5 14 2908.0 15 3040.5 16 3214.0 17 3468.5 18 3845.5 19 4204.0 20 4598.0 21 5320.0 22 6470.5 23 8220.5 24 10243.0 25 12870.0 26 16624.0 27 20860.5 28 24286.0 29 27368.0 30 30740.5 31 35190.5 32 41692.5 33 45951.5 34 47623.5 35 50863.5 36 55696.0 37 60727.0 38 65227.5 39 68468.0 40 72557.0 41 78368.5 42 82517.0 43 86053.5 44 89965.5 45 93724.5 46 95998.5 47 100163.5 48 104378.5 49 103667.5 50 102558.5 51 101342.0 52 100355.0 53 101143.5 54 102301.5 55 97827.5 56 89813.0 57 82556.5 58 76328.0 59 68705.0 60 56637.0 61 46576.0 62 38796.0 63 28869.0 64 20247.0 65 15289.0 66 11371.0 67 8473.0 68 6530.0 69 4796.0 70 3310.0 71 2122.5 72 1425.0 73 1031.5 74 682.0 75 465.5 76 324.0 77 207.0 78 126.0 79 76.0 80 53.0 81 29.0 82 14.0 83 8.5 84 7.0 85 5.5 86 3.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009091251535733327 2 0.013727427617700922 3 0.00854794965112775 4 3.622012564037182E-5 5 0.0011952641461322703 6 0.001448805025614873 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.787327012679943E-4 14-15 0.0 16-17 0.002988160365330675 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0039842138204409005 26-27 0.020573031363731193 28-29 0.0065558427409073 30-31 0.003223591181993092 32-33 0.0 34-35 0.009091251535733327 36-37 0.001032273580750597 38-39 0.003078710679431605 40-41 0.0023180880409837965 42-43 0.003404691810194951 44-45 0.0 46-47 9.055031410092956E-5 48-49 6.15742135886321E-4 50-51 0.004219644637103318 52-53 0.00814952826908366 54-55 0.007642446510118455 56-57 0.0169691288625142 58-59 0.024521025058531723 60-61 0.0037125628781381117 62-63 0.020971452745775287 64-65 0.02205805651498644 66-67 0.013962858434363337 68-69 0.03219969169429055 70-71 0.017295109993277547 72-73 0.005125147778112613 74-75 0.017114009365075684 76-77 0.02165963513294235 78-79 0.027708396114884445 80-81 0.012459723220287908 82-83 0.036256345766012193 84-85 0.022746238902153503 86-87 0.03245323257377315 88-89 0.02368796216880317 90-91 0.029229641391780057 92-93 0.025444638262361208 94-95 0.015248672894596537 96-97 0.015791974779202113 98-99 0.011590440204918983 100-101 0.01084792762929136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2760896.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.44562327202101 #Duplication Level Percentage of deduplicated Percentage of total 1 70.91707763092828 35.065391040915486 2 15.28391937387541 15.114458389611734 3 5.68921699419898 8.439206406238275 4 2.7244871846675722 5.3885586777008765 5 1.4676703177914463 3.6284936810521606 6 0.910231106144287 2.700416661893123 7 0.6118446629883223 2.117712849498207 8 0.43248602609012493 1.7107632893172613 9 0.3202838453194419 1.4252970920201438 >10 1.4962435128279226 13.042630392212882 >50 0.09179478314365241 3.124260595478799 >100 0.04873465470408755 4.389214930928269 >500 0.0033714114236984734 1.1050500072418468 >1k 0.0024186212386860238 1.8188587499587863 >5k 1.4658310537491053E-4 0.5412001707886933 >10k+ 7.329155268745526E-5 0.388487065143618 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10720 0.3882797468647859 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9025 0.3268866339043557 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5909 0.21402472240895706 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.622012564037182E-5 2 0.0 0.0 0.0 0.0 3.622012564037182E-5 3 0.0 0.0 0.0 0.0 3.622012564037182E-5 4 0.0 0.0 0.0 0.0 3.622012564037182E-5 5 0.0 0.0 0.0 0.0 3.622012564037182E-5 6 0.0 0.0 0.0 3.622012564037182E-5 3.622012564037182E-5 7 3.622012564037182E-5 0.0 0.0 7.244025128074364E-5 3.622012564037182E-5 8 3.622012564037182E-5 0.0 0.0 1.0866037692111546E-4 3.622012564037182E-5 9 3.622012564037182E-5 0.0 0.0 1.8110062820185911E-4 3.622012564037182E-5 10-11 3.622012564037182E-5 0.0 0.0 2.8976100512297456E-4 3.622012564037182E-5 12-13 7.244025128074364E-5 0.0 0.0 2.8976100512297456E-4 3.0787106794316046E-4 14-15 1.8110062820185911E-4 0.0 0.0 3.2598113076334637E-4 3.622012564037182E-4 16-17 1.8110062820185911E-4 0.0 0.0 3.2598113076334637E-4 4.3464150768446184E-4 18-19 2.1732075384223095E-4 0.0 0.0 3.9842138204409003E-4 4.3464150768446184E-4 20-21 2.5354087948260275E-4 0.0 0.0 5.070817589652055E-4 4.708616333248337E-4 22-23 2.7165094230278866E-4 0.0 0.0 5.795220102459491E-4 5.433018846055773E-4 24-25 3.2598113076334637E-4 0.0 0.0 7.606226384478083E-4 5.433018846055773E-4 26-27 3.2598113076334637E-4 0.0 0.0 0.0015031352140754306 5.433018846055773E-4 28-29 4.708616333248337E-4 0.0 0.0 0.003966103757620715 5.614119474257632E-4 30-31 5.070817589652055E-4 0.0 0.0 0.011554220079278612 6.157421358863209E-4 32-33 5.070817589652055E-4 0.0 0.0 0.030298135098171027 6.519622615266927E-4 34-35 5.433018846055773E-4 0.0 0.0 0.056992367695125064 6.519622615266927E-4 36-37 6.157421358863209E-4 0.0 0.0 0.09964156563666288 6.519622615266927E-4 38-39 7.244025128074365E-4 0.0 0.0 0.16125199935093534 6.519622615266927E-4 40-41 7.787327012679942E-4 0.0 0.0 0.22923717517791325 6.519622615266927E-4 42-43 8.692830153689238E-4 0.0 0.0 0.29999319061637963 6.519622615266927E-4 44-45 0.0010684937063909687 0.0 0.0 0.38474828461484967 6.519622615266927E-4 46-47 0.0013763647743341293 0.0 0.0 0.481999321959248 6.700723243468786E-4 48-49 0.001412584899974501 0.0 0.0 0.5831983529984468 7.062924499872506E-4 50-51 0.001412584899974501 0.0 0.0 0.6877115255337398 7.606226384478083E-4 52-53 0.001412584899974501 0.0 0.0 0.7903593615985536 7.606226384478083E-4 54-55 0.001412584899974501 0.0 0.0 0.9033299334708732 8.330628897285519E-4 56-57 0.0015212452768956165 0.0 0.0 1.0327806625095621 8.511729525487378E-4 58-59 0.0015212452768956165 0.0 0.0 1.1718478349057697 8.692830153689237E-4 60-61 0.0015212452768956165 0.0 0.0 1.3157866142006074 8.873930781891096E-4 62-63 0.0015212452768956165 0.0 0.0 1.4551797677275782 9.417232666496674E-4 64-65 0.0015212452768956165 3.622012564037182E-5 0.0 1.6002594809800876 9.417232666496674E-4 66-67 0.0015574654025359883 3.622012564037182E-5 0.0 1.7675964614386055 9.598333294698533E-4 68-69 0.0015936855281763601 3.622012564037182E-5 0.0 1.9529891745288486 9.960534551102251E-4 70-71 0.0016480157166369178 3.622012564037182E-5 0.0 2.1498455573842694 0.0010684937063909687 72-73 0.0017204559679176615 3.622012564037182E-5 0.0 2.345923207538422 0.0011590440204918982 74-75 0.0017385660307378474 3.622012564037182E-5 0.0 2.544735477178423 0.0012495943345928278 76-77 0.0017385660307378474 3.622012564037182E-5 0.0 2.756460221609217 0.0012677043974130137 78-79 0.0017385660307378474 3.622012564037182E-5 0.0 2.995621711212592 0.0013039245230533855 80-81 0.0021732075384223093 3.622012564037182E-5 0.0 3.243150049838893 0.0013763647743341293 82-83 0.0021732075384223093 3.622012564037182E-5 0.0 3.4999145205034887 0.0015393553397158024 84-85 0.002227537726882867 5.433018846055773E-5 0.0 3.76301751315515 0.001629905653816732 86-87 0.0023180880409837965 7.244025128074364E-5 0.0 4.03291177936438 0.0016842358422772897 88-89 0.00239052829226454 7.244025128074364E-5 0.0 4.320517687011753 0.0017385660307378474 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5580 0.0 52.014843 1 GTATCAA 9405 0.0 41.366177 1 TATCAAC 12260 0.0 31.577059 2 ATCAACG 12275 0.0 31.572023 3 TCAACGC 12415 0.0 31.136665 4 CAACGCA 12685 0.0 30.47392 5 TGGTATC 2395 0.0 30.345232 2 AACGCAG 13235 0.0 29.206474 6 ACGCAGA 14725 0.0 25.962282 7 CGCAGAG 14840 0.0 25.601086 8 GTGGTAT 2970 0.0 25.270912 1 GCGTAAT 100 0.0016536401 23.751457 1 TAAGGTG 1575 0.0 22.9154 5 GCAGAGT 16680 0.0 22.264502 9 TACATGG 9100 0.0 22.184626 2 GTACATG 9675 0.0 21.652493 1 GTAAGGT 1615 0.0 21.465685 4 GAGTACT 10385 0.0 21.35603 12-13 ACATGGG 9100 0.0 21.032814 3 AGAGTAC 14815 0.0 20.627995 10-11 >>END_MODULE