FastQCFastQC Report
Thu 26 May 2016
SRR938135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403188
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91180.3794126801565254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72950.30355511096094023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45220.1881667185422031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45250.058.208931
GTATCAA77400.043.462761
ATCAACG97500.034.120353
TCAACGC98750.033.7365724
TATCAAC99150.033.64842
CAACGCA100850.033.08125
AACGCAG103350.032.3215756
ACGCAGA119150.028.0291257
CGCAGAG118800.027.7917968
GCAGAGT126400.025.5554189
GTACATG73850.024.2944161
TGGTATC17550.024.1008822
GAGTACT83500.023.89323212-13
TACATGG73900.023.5373042
GTGGTAT18800.023.3506571
CAGAGTA121150.023.25133310-11
GTACTTT87350.022.59587914-15
ACATGGG74500.022.1356983
GGACCGA7700.021.598576
AGAGTAC117000.021.35583110-11
Produced by FastQC (version 0.11.5)