##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938135_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2403188 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.894539253691345 33.0 31.0 34.0 30.0 34.0 2 32.06238796132471 34.0 31.0 34.0 30.0 34.0 3 32.15558541404168 34.0 31.0 34.0 30.0 34.0 4 35.680453630760475 37.0 35.0 37.0 35.0 37.0 5 35.685594302235195 37.0 35.0 37.0 35.0 37.0 6 35.71701298441903 37.0 35.0 37.0 35.0 37.0 7 35.64820272071931 37.0 35.0 37.0 33.0 37.0 8 35.61360992148762 37.0 35.0 37.0 33.0 37.0 9 37.250254245610414 39.0 37.0 39.0 34.0 39.0 10-11 37.149525963012465 39.0 37.0 39.0 34.0 39.0 12-13 37.01632851861777 39.0 37.0 39.0 33.0 39.0 14-15 38.19462543088598 40.0 38.0 41.0 33.0 41.0 16-17 38.015109304806785 40.0 38.0 41.0 33.0 41.0 18-19 38.19387413718777 40.0 38.0 41.0 33.5 41.0 20-21 38.17786706657989 40.0 38.0 41.0 33.5 41.0 22-23 38.068996682739765 40.0 38.0 41.0 33.0 41.0 24-25 37.943902016820985 40.0 38.0 41.0 33.0 41.0 26-27 37.74540859891111 40.0 38.0 41.0 32.5 41.0 28-29 37.53568260161086 40.0 37.0 41.0 32.0 41.0 30-31 37.28241090584673 40.0 37.0 41.0 31.5 41.0 32-33 36.97283545856587 39.5 36.0 41.0 30.5 41.0 34-35 36.68394794747643 39.0 36.0 41.0 30.0 41.0 36-37 36.38274845746567 39.0 35.5 41.0 30.0 41.0 38-39 36.05911314470612 39.0 35.0 40.0 29.5 41.0 40-41 35.90455906903663 39.0 35.0 40.0 28.5 41.0 42-43 35.638017500087386 39.0 35.0 40.0 27.0 41.0 44-45 35.28069859703028 38.0 35.0 40.0 26.0 41.0 46-47 34.83092042736565 38.0 34.0 40.0 24.5 41.0 48-49 34.35268776308803 38.0 33.5 40.0 23.0 41.0 50-51 33.52712896369323 37.5 32.5 39.5 21.5 40.5 52-53 33.494579908022175 38.0 33.0 39.5 20.5 40.5 54-55 33.47154737789969 38.0 33.0 40.0 18.5 41.0 56-57 32.99046578960947 37.5 32.0 40.0 13.5 41.0 58-59 32.52317775388359 37.0 31.5 40.0 8.5 41.0 60-61 31.95269242356403 36.5 31.0 39.5 4.5 41.0 62-63 31.316315868754337 36.0 30.0 39.0 2.0 41.0 64-65 30.691275505703256 35.0 29.0 39.0 2.0 40.0 66-67 29.992174353400564 35.0 28.0 38.0 2.0 40.0 68-69 29.27196166092707 34.5 27.5 38.0 2.0 40.0 70-71 28.4209587431362 34.0 26.0 37.0 2.0 39.5 72-73 27.606729477677153 34.0 24.5 36.5 2.0 39.0 74-75 26.744274896512465 33.0 21.5 36.0 2.0 39.0 76-77 25.885929024279413 32.5 19.0 35.0 2.0 37.5 78-79 25.029972686281724 32.0 13.0 35.0 2.0 37.0 80-81 24.209387696676245 31.0 4.5 35.0 2.0 36.5 82-83 23.359522642423315 30.5 2.0 34.5 2.0 36.0 84-85 22.526858073525666 30.0 2.0 34.0 2.0 35.5 86-87 21.734608986063513 29.0 2.0 34.0 2.0 35.0 88-89 20.88785896068056 29.0 2.0 34.0 2.0 35.0 90-91 20.01826532089874 27.0 2.0 34.0 2.0 35.0 92-93 19.085021022075676 25.5 2.0 34.0 2.0 35.0 94-95 18.12995383632075 24.5 2.0 33.0 2.0 35.0 96-97 17.000503913967613 18.5 2.0 32.5 2.0 35.0 98-99 15.677309057801553 2.0 2.0 32.0 2.0 34.0 100-101 12.651019812016372 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5233565962092612 1101 2 0.45131925081894053 1101 3 0.42897433064927526 1101 4 0.16227314309655583 1101 5 0.34832108881057877 1101 6 0.26138634706931896 1101 7 0.30922025879340964 1101 8 0.23497601644727695 1101 9 0.35840413826260686 1101 10-11 0.43806188032244364 1101 12-13 0.45008623767638056 1101 14-15 0.5675473852680142 1101 16-17 0.5482117019100556 1101 18-19 0.5790590617926554 1101 20-21 0.40919360713024844 1101 22-23 0.5648539275451512 1101 24-25 0.6197088819117269 1101 26-27 0.6420438952596115 1101 28-29 0.8576610537210669 1101 30-31 0.9597972074627137 1101 32-33 0.8989786162225357 1101 34-35 1.1174152150942902 1101 36-37 1.114406191254858 1101 38-39 1.278658784565195 1101 40-41 1.3708294866488089 1101 42-43 1.447928379280519 1101 44-45 1.5875190739913876 1101 46-47 1.7765996157091237 1101 48-49 1.6957513882537754 1101 50-51 1.963196515525624 1101 52-53 2.114488302166464 1101 54-55 2.2029090234634836 1101 56-57 2.342316282815837 1101 58-59 2.567658355013947 1101 60-61 2.4849225616738124 1101 62-63 2.6240495723216135 1101 64-65 2.806205569580385 1101 66-67 2.8673051428663676 1101 68-69 3.1171083471846437 1101 70-71 3.5492405830364895 1101 72-73 3.7985234621345967 1101 74-75 3.98462529644911 1101 76-77 4.288486430363964 1101 78-79 4.625204981768945 1101 80-81 4.7851694713547275 1101 82-83 5.11536261491867 1101 84-85 5.314311219485301 1101 86-87 5.474613516721991 1101 88-89 5.570396674920229 1101 90-91 5.318288323787275 1101 92-93 5.441539471003107 1101 94-95 5.5200785037318205 1101 96-97 5.445874237454895 1101 98-99 5.428754470929556 1101 100-101 4.638064072583111 1102 1 0.5892506161143132 1102 2 0.4986392116066227 1102 3 0.43454946179312515 1102 4 0.3197922461551528 1102 5 0.2647062608554478 1102 6 0.35008642190075534 1102 7 0.422352232428139 1102 8 0.3924570833375185 1102 9 0.4619461417921471 1102 10-11 0.5518976579516774 1102 12-13 0.6115397911986591 1102 14-15 0.849708988808132 1102 16-17 0.8929921684762618 1102 18-19 0.7841279612093714 1102 20-21 0.7268753269477415 1102 22-23 0.8182218818844831 1102 24-25 0.7342825881829498 1102 26-27 0.8612045206497498 1102 28-29 0.8975589711194587 1102 30-31 0.974126187408217 1102 32-33 0.9892571920404905 1102 34-35 1.2300141428520064 1102 36-37 1.1688869328144662 1102 38-39 1.3686051508702235 1102 40-41 1.3728085813232624 1102 42-43 1.5047223133479264 1102 44-45 1.605547543389008 1102 46-47 1.7121435517997199 1102 48-49 1.8618959343258226 1102 50-51 1.9965072969180042 1102 52-53 1.9853426970164207 1102 54-55 2.0917864925098613 1102 56-57 2.5949418574722074 1102 58-59 2.577799310040092 1102 60-61 2.7298655047236835 1102 62-63 2.9341380859922417 1102 64-65 2.7988618914883006 1102 66-67 3.076998673858288 1102 68-69 3.3594232877376164 1102 70-71 3.493217758098357 1102 72-73 3.630953503909641 1102 74-75 3.78218156704866 1102 76-77 4.060259978663698 1102 78-79 4.393006987596845 1102 80-81 4.606974878317452 1102 82-83 4.739979488194567 1102 84-85 4.826945162098706 1102 86-87 5.19786141164937 1102 88-89 5.1904558171156125 1102 90-91 5.430371740071823 1102 92-93 5.528506472334104 1102 94-95 5.316260751469748 1102 96-97 5.234810595122202 1102 98-99 4.721637553143248 1102 100-101 4.074446288057734 1103 1 0.46958516382136395 1103 2 0.32096979358897215 1103 3 0.2581403954461834 1103 4 0.23636525169094824 1103 5 0.2020193649002735 1103 6 0.1829328184040051 1103 7 0.20353658911196248 1103 8 0.22181875525754435 1103 9 0.28165370685789526 1103 10-11 0.3396060839672117 1103 12-13 0.42373210838388076 1103 14-15 0.639442086794979 1103 16-17 0.6820578282762924 1103 18-19 0.6642139248226258 1103 20-21 0.6084074487367701 1103 22-23 0.5993985334718914 1103 24-25 0.7491361193528476 1103 26-27 0.7317815111098298 1103 28-29 0.7051282304626412 1103 30-31 0.8620003614155678 1103 32-33 0.811881072938391 1103 34-35 0.7872061682540661 1103 36-37 0.8528444673756184 1103 38-39 1.0917778367329305 1103 40-41 1.0992002290998073 1103 42-43 1.1685427679284075 1103 44-45 1.1531846176855822 1103 46-47 1.2818102545919245 1103 48-49 1.3331978606757175 1103 50-51 1.445377287689574 1103 52-53 1.551335079328652 1103 54-55 1.6470143498943344 1103 56-57 1.910319410985366 1103 58-59 2.1085369606020947 1103 60-61 2.3596598746476403 1103 62-63 2.453509582541752 1103 64-65 2.592723531703747 1103 66-67 2.8942110508199015 1103 68-69 2.948839542055314 1103 70-71 3.1826743315434634 1103 72-73 3.31852611812759 1103 74-75 3.5666873255345592 1103 76-77 3.8121628216926915 1103 78-79 3.7624767178098395 1103 80-81 3.926333945662268 1103 82-83 4.192945179800233 1103 84-85 4.522996161247608 1103 86-87 4.596052583427802 1103 88-89 4.933527609161882 1103 90-91 5.0917321215776745 1103 92-93 5.05942323480922 1103 94-95 5.115251585941792 1103 96-97 5.188629278793638 1103 98-99 5.164278920820598 1103 100-101 4.1410827406414565 1104 1 0.5355153207804904 1104 2 0.47614892337204395 1104 3 0.3627485822841052 1104 4 0.365855999182088 1104 5 0.2976282709199154 1104 6 0.32075085908813605 1104 7 0.3526396546856958 1104 8 0.38834066987285354 1104 9 0.48845472878625174 1104 10-11 0.5141823408270838 1104 12-13 0.46068121412613294 1104 14-15 0.6331469574373543 1104 16-17 0.7871654273011401 1104 18-19 0.672971952221971 1104 20-21 0.7791042801812864 1104 22-23 0.7701295278093099 1104 24-25 0.7913198959937304 1104 26-27 0.8315861134502356 1104 28-29 0.9057683092415729 1104 30-31 0.9546053982234355 1104 32-33 1.1830756964738782 1104 34-35 1.2474620447999385 1104 36-37 1.1968154916073175 1104 38-39 1.2580525106181852 1104 40-41 1.3781973051788086 1104 42-43 1.3504570028662712 1104 44-45 1.4322345229804085 1104 46-47 1.6533602426525178 1104 48-49 1.9197701532312337 1104 50-51 1.9162645156279154 1104 52-53 1.95631469077329 1104 54-55 1.9422106245189141 1104 56-57 2.3347077719342906 1104 58-59 2.5303469036240216 1104 60-61 2.5470760963162107 1104 62-63 2.6009840994890148 1104 64-65 2.807615876327745 1104 66-67 2.7864862924431257 1104 68-69 2.8861249233660295 1104 70-71 3.2314638733310197 1104 72-73 3.595322329075355 1104 74-75 3.8981719885088175 1104 76-77 3.9135170810306086 1104 78-79 4.181125712751623 1104 80-81 4.463212612450089 1104 82-83 4.5851651268442275 1104 84-85 4.6962914857211295 1104 86-87 4.76427735976651 1104 88-89 5.086890118310141 1104 90-91 5.1839511529152595 1104 92-93 5.356455852975849 1104 94-95 5.389984287719461 1104 96-97 5.031997345575402 1104 98-99 5.050764432584254 1104 100-101 4.32931746296053 1105 1 0.5302130544671755 1105 2 0.5441148700405876 1105 3 0.44252492162914336 1105 4 0.4211809364460919 1105 5 0.4263953620805907 1105 6 0.43362092327038226 1105 7 0.4654549958835048 1105 8 0.379373917994684 1105 9 0.5273979497811112 1105 10-11 0.4955560049999477 1105 12-13 0.36861174720618095 1105 14-15 0.641490258171423 1105 16-17 0.680020755365426 1105 18-19 0.6533596254788492 1105 20-21 0.7884521385581991 1105 22-23 0.8119701353907871 1105 24-25 0.7772091835786554 1105 26-27 0.803719410822751 1105 28-29 0.9317245933945841 1105 30-31 0.9083263657258058 1105 32-33 0.9750130464742739 1105 34-35 1.0874754910189708 1105 36-37 1.1646696470362343 1105 38-39 1.1619941290113616 1105 40-41 1.2343970284368595 1105 42-43 1.2307856534756425 1105 44-45 1.39460953959275 1105 46-47 1.5394836664351317 1105 48-49 1.5665108131904262 1105 50-51 1.5233036503793542 1105 52-53 1.5982866335174108 1105 54-55 1.5876183889286537 1105 56-57 2.0361645759556453 1105 58-59 2.256801362374617 1105 60-61 2.3924602125791026 1105 62-63 2.484536520199317 1105 64-65 2.742574677738034 1105 66-67 2.815660483520144 1105 68-69 2.82667133236561 1105 70-71 3.056890712609615 1105 72-73 3.368664613525425 1105 74-75 3.577642856337178 1105 76-77 3.8099313574754916 1105 78-79 4.0130538380203475 1105 80-81 4.256746700960608 1105 82-83 4.485505777422336 1105 84-85 4.415073000296989 1105 86-87 4.578369700893962 1105 88-89 4.785987970823879 1105 90-91 4.959996361917973 1105 92-93 4.917890220573586 1105 94-95 4.970459322312202 1105 96-97 4.940044164997371 1105 98-99 4.683586887770039 1105 100-101 4.123924512844438 1106 1 0.3772494955144765 1106 2 0.33239696688146836 1106 3 0.3275054215900326 1106 4 0.2782016689068314 1106 5 0.3496467840748423 1106 6 0.29381450286454225 1106 7 0.2588240247448397 1106 8 0.18762699226405033 1106 9 0.32503735151384916 1106 10-11 0.3212555479503223 1106 12-13 0.4040295014210713 1106 14-15 0.6414842716674016 1106 16-17 0.4992840082880434 1106 18-19 0.6415614233457418 1106 20-21 0.5802076050345732 1106 22-23 0.5461308434904524 1106 24-25 0.5515443224416003 1106 26-27 0.7399306162811641 1106 28-29 0.8071705879444622 1106 30-31 0.8060810675260655 1106 32-33 0.8073374543292005 1106 34-35 0.8330809979641103 1106 36-37 0.9931195894859712 1106 38-39 0.9520889392711069 1106 40-41 1.239778496455358 1106 42-43 1.0977383952146695 1106 44-45 1.3998686833789975 1106 46-47 1.516162242253806 1106 48-49 1.5581338981077906 1106 50-51 1.6518620283720153 1106 52-53 1.6822263997085116 1106 54-55 1.7135606646622321 1106 56-57 1.7096550590106432 1106 58-59 2.1848250258277275 1106 60-61 2.412185344242282 1106 62-63 2.17673063778064 1106 64-65 2.5713437008523243 1106 66-67 2.7385521166224827 1106 68-69 2.649184458773437 1106 70-71 2.9537697884211056 1106 72-73 3.2210096867922537 1106 74-75 3.478948353224993 1106 76-77 3.2588271667630337 1106 78-79 3.5149575463004012 1106 80-81 3.68812899272206 1106 82-83 3.7913257369056765 1106 84-85 3.747483414259751 1106 86-87 3.882126112439771 1106 88-89 3.9969747222913696 1106 90-91 4.212942320548837 1106 92-93 4.259979813794633 1106 94-95 4.201162624787106 1106 96-97 4.300411552758405 1106 98-99 4.161231286238133 1106 100-101 3.169996647823343 1107 1 0.5055813835776277 1107 2 0.4304700300168136 1107 3 0.4423017465516068 1107 4 0.29003339546248696 1107 5 0.3746175409912027 1107 6 0.3706025374939159 1107 7 0.3364682227665341 1107 8 0.36512199363599507 1107 9 0.4200176522303849 1107 10-11 0.4568934794039947 1107 12-13 0.46367410388687347 1107 14-15 0.6418024626599532 1107 16-17 0.5561991919608502 1107 18-19 0.5249930504884119 1107 20-21 0.6111915735342421 1107 22-23 0.5000396529235687 1107 24-25 0.637226075104742 1107 26-27 0.6185998948540075 1107 28-29 0.6505943770531104 1107 30-31 0.7372139432355311 1107 32-33 0.7066332855062001 1107 34-35 0.8859117014459983 1107 36-37 0.8944076358858979 1107 38-39 1.0337785430895536 1107 40-41 1.0576796631299388 1107 42-43 1.0109816625518349 1107 44-45 0.9691930263213493 1107 46-47 1.1708904755007339 1107 48-49 1.2325287081020306 1107 50-51 1.2441251632537345 1107 52-53 1.4177611554655698 1107 54-55 1.4319934746495377 1107 56-57 1.5415069421648937 1107 58-59 1.6759449114719303 1107 60-61 1.8624609103771874 1107 62-63 1.8905482311761652 1107 64-65 2.1229332857992773 1107 66-67 2.2280666717860775 1107 68-69 2.350324582059425 1107 70-71 2.6201855106451966 1107 72-73 2.653207122662973 1107 74-75 2.9985128541475348 1107 76-77 3.022766691564371 1107 78-79 3.15628712154534 1107 80-81 3.365543047386076 1107 82-83 3.3964562145086212 1107 84-85 3.618128934829091 1107 86-87 3.7535200282575083 1107 88-89 4.070553639639282 1107 90-91 4.188218745355616 1107 92-93 4.369943070356911 1107 94-95 4.31704811230626 1107 96-97 4.398337194475733 1107 98-99 4.157770163234256 1107 100-101 3.5264607682674107 1108 1 0.42236873492051075 1108 2 0.45488848104003665 1108 3 0.385114780221798 1108 4 0.3056374971120661 1108 5 0.34452519242912416 1108 6 0.3465863360055721 1108 7 0.3085897190091629 1108 8 0.3103341935169084 1108 9 0.3971530348179826 1108 10-11 0.38350237447811253 1108 12-13 0.38644602133325634 1108 14-15 0.4729470795148174 1108 16-17 0.49847019978145823 1108 18-19 0.5214769498078482 1108 20-21 0.5300199142814179 1108 22-23 0.5258353521712635 1108 24-25 0.508551867191791 1108 26-27 0.603338362964486 1108 28-29 0.5384031732629708 1108 30-31 0.6911379178771284 1108 32-33 0.6842771240700642 1108 34-35 0.8415381201903145 1108 36-37 1.057367187329568 1108 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2311 44-45 0.6854374303349715 2311 46-47 0.7579040347934125 2311 48-49 0.7485435960957076 2311 50-51 0.7813983852943309 2311 52-53 0.8277823928732957 2311 54-55 1.0722224230129953 2311 56-57 1.2423329785468624 2311 58-59 1.366506387866238 2311 60-61 1.551804090145012 2311 62-63 1.7402608590952973 2311 64-65 1.9309671001653541 2311 66-67 1.9930867979340938 2311 68-69 2.1558487614991613 2311 70-71 2.6061544537426045 2311 72-73 2.800482147093046 2311 74-75 3.098826011302105 2311 76-77 3.188321617459547 2311 78-79 3.551500222030473 2311 80-81 3.693343333651711 2311 82-83 3.977725459105379 2311 84-85 4.065847533083211 2311 86-87 4.153674466096401 2311 88-89 4.482330523064132 2311 90-91 4.741308175624152 2311 92-93 4.9689905460014 2311 94-95 5.09157720598337 2311 96-97 5.031721312217304 2311 98-99 4.951327214153041 2311 100-101 4.469459399156969 2312 1 -0.2546566276774165 2312 2 -0.2080456502771746 2312 3 -0.08863072547791973 2312 4 -0.09914636020941714 2312 5 -0.10674247062809883 2312 6 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2312 68-69 2.3021238447870545 2312 70-71 2.7559353821181958 2312 72-73 3.040877060716415 2312 74-75 2.754570683228522 2312 76-77 2.8143060415750902 2312 78-79 3.164747540992913 2312 80-81 3.628063421509154 2312 82-83 3.96922462845086 2312 84-85 4.190936540860985 2312 86-87 4.389251456394202 2312 88-89 4.709303089220963 2312 90-91 4.772734181275396 2312 92-93 4.903781751105065 2312 94-95 5.084120762913393 2312 96-97 4.968954654321134 2312 98-99 4.830547568302945 2312 100-101 4.292477998954359 2313 1 -0.39636158372696073 2313 2 -0.3003830858112657 2313 3 -0.3643098910097038 2313 4 -0.17409445220837227 2313 5 -0.22140079166313598 2313 6 -0.2947956779005523 2313 7 -0.2276973400690423 2313 8 -0.219255843142534 2313 9 -0.2439550527105112 2313 10-11 -0.1666810288577878 2313 12-13 -0.10080670023217664 2313 14-15 -0.06965915942750911 2313 16-17 -0.16331182546372958 2313 18-19 -0.17533930410719734 2313 20-21 -0.2319548144564294 2313 22-23 -0.20274650808141104 2313 24-25 -0.11810633863137099 2313 26-27 -0.11621840384321303 2313 28-29 -0.06294981384581178 2313 30-31 -0.11946124415374015 2313 32-33 0.00736995145869912 2313 34-35 0.11593625148791631 2313 36-37 0.17942678018735592 2313 38-39 0.1353827326918804 2313 40-41 0.5225088870973806 2313 42-43 0.4339466045170397 2313 44-45 0.5513926214410461 2313 46-47 0.5152017814710277 2313 48-49 0.7084119638714839 2313 50-51 0.8908965939341087 2313 52-53 1.0240481491536997 2313 54-55 1.1302976846590767 2313 56-57 1.424598091438341 2313 58-59 1.5353205735272866 2313 60-61 1.6850220650151968 2313 62-63 1.7722127470752973 2313 64-65 2.061918788293589 2313 66-67 2.1897870522435987 2313 68-69 2.4236397779769057 2313 70-71 2.5184373626035175 2313 72-73 2.8037141363510756 2313 74-75 3.0510321567808134 2313 76-77 3.1430041788210907 2313 78-79 3.1139409568212457 2313 80-81 3.5283527058819217 2313 82-83 3.875384457028357 2313 84-85 4.178891748365405 2313 86-87 4.319097177329429 2313 88-89 4.383982903343146 2313 90-91 4.717440811317179 2313 92-93 4.815208394818871 2313 94-95 4.728860504889532 2313 96-97 4.6901232089021185 2313 98-99 4.693575838655365 2313 100-101 4.0923551050187665 2314 1 -0.4288481075625512 2314 2 -0.3651740239130348 2314 3 -0.33025731491902377 2314 4 -0.323339659817222 2314 5 -0.302986216414574 2314 6 -0.32813422240388945 2314 7 -0.3005888899936835 2314 8 -0.25949643036823034 2314 9 -0.2844084762747485 2314 10-11 -0.4189850581952115 2314 12-13 -0.329417801870143 2314 14-15 -0.34616310815499673 2314 16-17 -0.28561528416116033 2314 18-19 -0.3369500103490424 2314 20-21 -0.38140756844723 2314 22-23 -0.3445460455141003 2314 24-25 -0.304193674013824 2314 26-27 -0.23268265088373852 2314 28-29 -0.2665256331616064 2314 30-31 -0.25802895576691753 2314 32-33 -0.14494551318914262 2314 34-35 -0.09963640150747466 2314 36-37 -0.04599413997020463 2314 38-39 0.0479744163623792 2314 40-41 0.04933363792635248 2314 42-43 0.11594834885209337 2314 44-45 0.2864235356096998 2314 46-47 0.35119286778162717 2314 48-49 0.41731949666836954 2314 50-51 0.3925000135421328 2314 52-53 0.7561128608550121 2314 54-55 0.9852794337460722 2314 56-57 1.2119201702201963 2314 58-59 1.312784480547407 2314 60-61 1.2162230168472163 2314 62-63 1.4186902944173134 2314 64-65 1.534444385774492 2314 66-67 1.638672396486399 2314 68-69 2.0038914020779934 2314 70-71 2.2502595804437036 2314 72-73 2.5400172850795535 2314 74-75 2.764254380766353 2314 76-77 2.980351576235609 2314 78-79 3.2365264152258106 2314 80-81 3.550471139906204 2314 82-83 3.7908526390087154 2314 84-85 3.976489849203457 2314 86-87 4.116082487345086 2314 88-89 4.403411000508616 2314 90-91 4.312437063889419 2314 92-93 4.261561932072151 2314 94-95 4.338428393097217 2314 96-97 4.275568219747093 2314 98-99 4.286225882927797 2314 100-101 3.9075253724013113 2315 1 -0.5210943601006512 2315 2 -0.47604927037038536 2315 3 -0.39930013258898 2315 4 -0.36133978709001724 2315 5 -0.25208864908967143 2315 6 -0.12121015453005413 2315 7 -0.2498929270546597 2315 8 -0.2915066079137816 2315 9 -0.3894086393764411 2315 10-11 -0.3396471548059523 2315 12-13 -0.2696583046725536 2315 14-15 -0.32332651840280846 2315 16-17 -0.24086322814401484 2315 18-19 -0.34287482635161837 2315 20-21 -0.42711839503055415 2315 22-23 -0.3259289232589495 2315 24-25 -0.2787006657287989 2315 26-27 -0.2243166571051347 2315 28-29 -0.1834077812269257 2315 30-31 -0.19439020515795846 2315 32-33 -0.22058373545557686 2315 34-35 -0.04763696193558786 2315 36-37 -6.195702511817558E-4 2315 38-39 0.03507045224261418 2315 40-41 0.19750398454151252 2315 42-43 0.3603012067947944 2315 44-45 0.392609795602354 2315 46-47 0.6230268099388141 2315 48-49 0.7121756436502693 2315 50-51 0.8354118011954981 2315 52-53 0.9546676729164076 2315 54-55 1.1286723981273425 2315 56-57 1.3488307247178284 2315 58-59 1.7045800376573368 2315 60-61 1.6942051975863492 2315 62-63 1.7637084468334372 2315 64-65 2.0705495387718997 2315 66-67 2.315597731740592 2315 68-69 2.4779805860829 2315 70-71 2.634921925182926 2315 72-73 2.9647733410988373 2315 74-75 3.352442609744017 2315 76-77 3.4503203302295944 2315 78-79 3.6213154445247575 2315 80-81 3.991810894633346 2315 82-83 4.1571410167030365 2315 84-85 4.382852793782572 2315 86-87 4.653219405600122 2315 88-89 4.606287472635074 2315 90-91 4.840500127024747 2315 92-93 5.051338611097112 2315 94-95 5.001484523073756 2315 96-97 5.090619671646266 2315 98-99 5.1679092421167585 2315 100-101 4.871042197811379 2316 1 -0.6348867420608713 2316 2 -0.6228914836376767 2316 3 -0.5747724627241553 2316 4 -0.5116478000543268 2316 5 -0.3732386379239472 2316 6 -0.42835329907887143 2316 7 -0.39321592622779633 2316 8 -0.3243951901085822 2316 9 -0.42355442144972244 2316 10-11 -0.42605228540465845 2316 12-13 -0.38792084800258664 2316 14-15 -0.45362862661172443 2316 16-17 -0.32614549959377115 2316 18-19 -0.5311601739073311 2316 20-21 -0.5114676748918185 2316 22-23 -0.45011965993502656 2316 24-25 -0.5863174481098881 2316 26-27 -0.3283184617271431 2316 28-29 -0.24678461728395718 2316 30-31 -0.2879432584295145 2316 32-33 -0.08567098903011328 2316 34-35 -0.03607531005260256 2316 36-37 0.20951286928794133 2316 38-39 -0.03701142166721638 2316 40-41 0.11744460503372522 2316 42-43 0.3406293180576867 2316 44-45 0.4949215526040618 2316 46-47 0.6068400848750883 2316 48-49 0.7404878392334737 2316 50-51 0.6703436098210673 2316 52-53 0.789905834736885 2316 54-55 1.1398414391025184 2316 56-57 1.2312606070049696 2316 58-59 1.4372185479287864 2316 60-61 1.4745649450599814 2316 62-63 1.6766408670588824 2316 64-65 2.203907634523489 2316 66-67 2.3340483822695965 2316 68-69 2.50956670196096 2316 70-71 2.7255348226976963 2316 72-73 3.1206205075203926 2316 74-75 3.2800546341194874 2316 76-77 3.70146572200559 2316 78-79 4.1178019138834046 2316 80-81 4.140981764372633 2316 82-83 4.272214941826313 2316 84-85 4.4922202305151835 2316 86-87 4.73698595551291 2316 88-89 4.978750992915444 2316 90-91 5.3222181191262585 2316 92-93 5.486426186566323 2316 94-95 5.762067011601232 2316 96-97 5.790565150736189 2316 98-99 5.78115078181945 2316 100-101 5.511757196292441 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6138.0 3 1936.0 4 2149.0 5 2523.0 6 3015.0 7 3747.0 8 4942.0 9 6287.0 10 7919.0 11 10237.0 12 13067.0 13 15945.0 14 19122.0 15 23113.0 16 27454.0 17 34338.0 18 35268.0 19 32534.0 20 35091.0 21 38559.0 22 43017.0 23 48288.0 24 53385.0 25 59645.0 26 65216.0 27 70991.0 28 78316.0 29 86132.0 30 94273.0 31 105190.0 32 119860.0 33 139066.0 34 166301.0 35 206987.0 36 258826.0 37 296074.0 38 175234.0 39 13003.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21524865626773 18.50657516002383 14.86264395042666 23.415532233281784 2 15.378938851346287 19.584875410179954 40.585339906201774 24.450845832271987 3 19.15825950265557 23.563996439808534 29.70911503931114 27.56862901822475 4 12.86051348142672 16.53579034479084 36.7040230315685 33.89967314221394 5 14.0023474227272 36.83621709371746 34.297936158262424 14.863499325292917 6 32.721668739365015 35.6595573182628 17.18509106064006 14.433682881732125 7 28.824808506706116 32.50815631147411 20.330881098086355 18.336154083733412 8 26.288782518210198 35.09107180020812 19.506805411030175 19.11334027055151 9 26.182455424690776 16.941718576670365 20.168623394570833 36.70720260406802 10-11 25.045682114089157 26.375562337459087 27.007682087449925 21.571073461001834 12-13 25.819584618644086 23.736392633072587 27.52220946219032 22.921813286093006 14-15 22.83018530241866 25.21825196151024 25.7188735763937 26.232689159677403 16-17 22.28505092246499 28.41164698419464 26.586592332243903 22.716709761096467 18-19 22.226136996952025 26.872560694154075 28.92733183799575 21.973970470898145 20-21 22.76920630533276 25.996135344530458 29.29345572796555 21.941202622171232 22-23 22.6434907908546 25.84581312332615 29.065054735848 22.44564134997125 24-25 22.46651406347929 26.163314829312217 29.073967029968273 22.29620407724022 26-27 22.06877891154693 26.433164568817936 28.969669218080874 22.528387301554265 28-29 22.3238451857653 26.685948344057444 28.893566514398717 22.096639955778542 30-31 22.300611026110506 26.329487041530086 28.883882976542402 22.48601895581701 32-33 22.11975955927943 26.240681735733745 28.907541547537953 22.732017157448876 34-35 22.643509816658174 26.43116455184577 28.68506335721322 22.240262274282838 36-37 22.68042288486802 26.047346854504493 28.716122839412645 22.55610742121484 38-39 22.297893875777564 26.48500475595233 28.62875208846758 22.58834927980253 40-41 22.76420920247518 26.301668361149062 28.58518280225911 22.34893963411665 42-43 22.352585478968965 26.21381405226433 28.544954619437092 22.888645849329624 44-45 22.431147026932774 26.49065352401169 28.579968617930064 22.498230831125472 46-47 22.693043849927314 26.689095970591087 28.002413009107258 22.615447170374342 48-49 22.60878431454151 26.667048156105476 28.052603925526327 22.671563603826687 50-51 22.7784696277847 26.948025938653192 27.813140156326888 22.46036427723522 52-53 22.796243428527912 26.758986482226778 27.748356234698125 22.69641385454719 54-55 22.672948594389542 26.81878785760298 27.8092442877569 22.699019260250576 56-57 22.662775167284604 27.03097420719925 27.733154446985463 22.573096178530683 58-59 22.72723206046802 26.657911387335183 27.7612263882621 22.8536301639347 60-61 22.585683782824418 26.856296433159343 27.819759131204457 22.73826065281178 62-63 22.516181492384273 26.84829387937183 28.024651131435245 22.610873496808644 64-65 22.432514255573484 26.77871375765577 27.8617732154394 22.92699877133134 66-67 22.45453109808025 26.828509110217496 27.673106200182808 23.043853591519447 68-69 22.572917043793296 26.628157096077093 27.958285321212017 22.840640538917594 70-71 22.601939137316336 26.583511235680323 27.701159016709674 23.113390610293663 72-73 22.642444642403444 26.621774227278433 27.54442684731847 23.191354282999658 74-75 22.565957551290143 26.976708183742716 27.389347330590315 23.06798693437683 76-77 22.747085008836837 26.705393113384098 27.437079927707302 23.110441950071763 78-79 22.77945643222423 26.66966008599908 27.400023886321083 23.150859595455607 80-81 22.83193563368395 26.805100799169036 27.240120249888054 23.122843317258955 82-83 22.91150287064056 26.771594724480096 27.12224733951796 23.19465506536138 84-85 22.95350167378494 26.66239854453845 27.199749155929258 23.184350625747346 86-87 23.02895456393386 26.55120571542944 27.14816499311953 23.271674727517176 88-89 22.960380600622614 26.604771825464958 27.03105584374747 23.403791730164965 90-91 22.98183034986872 26.562612177763036 26.968860857642113 23.486696614726128 92-93 23.024354737721968 26.28712759004353 27.046591391703164 23.641926280531337 94-95 22.876083588351406 26.288979785503873 27.084199146136022 23.7507374800087 96-97 23.11338629375868 26.003319130783115 27.020200916662763 23.863093658795435 98-99 22.93268857601317 26.11600096472209 26.7131880798139 24.238122379450836 100-101 23.762327157361888 24.996625835105814 26.874202801473302 24.366844206059 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 653.0 1 431.5 2 296.5 3 383.0 4 331.5 5 310.5 6 553.0 7 1321.0 8 2353.5 9 2775.0 10 2463.5 11 2138.5 12 2163.5 13 2311.0 14 2446.0 15 2605.0 16 2774.0 17 2930.0 18 3167.5 19 3507.5 20 4002.5 21 4882.5 22 6037.0 23 7350.0 24 8997.0 25 11438.0 26 14583.0 27 17519.5 28 19937.5 29 22676.5 30 26013.5 31 30348.5 32 35646.5 33 39084.0 34 42018.5 35 45880.0 36 50506.5 37 56220.5 38 60232.5 39 62575.5 40 65628.0 41 70239.0 42 73651.5 43 75611.0 44 78711.5 45 82157.0 46 84947.0 47 87684.0 48 88125.5 49 87389.5 50 87670.5 51 86731.5 52 85843.0 53 86603.0 54 87599.0 55 83590.5 56 76176.5 57 69574.5 58 64326.5 59 57495.0 60 47372.0 61 38961.0 62 32019.0 63 25055.0 64 19301.0 65 14979.5 66 11428.5 67 8693.0 68 6579.5 69 4848.0 70 3612.0 71 2647.5 72 1898.5 73 1398.0 74 1014.5 75 709.5 76 500.5 77 321.5 78 190.5 79 120.0 80 79.5 81 47.5 82 33.5 83 21.5 84 14.0 85 11.0 86 7.0 87 4.0 88 4.5 89 5.0 90 2.5 91 3.0 92 4.0 93 3.5 94 2.0 95 0.5 96 0.0 97 0.0 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3945592271599226 2 0.024301053434021808 3 0.04452419036712899 4 0.038282481437157644 5 0.058464006977398356 6 0.05521831833381325 7 0.031374990221322674 8 0.02862863829213528 9 0.03299783454311523 10-11 0.0297937572923966 12-13 0.030001814256728977 14-15 0.023926550898223528 16-17 0.029627311720930697 18-19 0.025341338255683698 20-21 0.03761669915129403 22-23 0.025112475594918082 24-25 0.04566850367095708 26-27 0.038511344097923256 28-29 0.0305635680604264 30-31 0.038178452954991456 32-33 0.03534887824007111 34-35 0.03401731366834389 36-37 0.03462067886490778 38-39 0.017039865378821798 40-41 0.012108915324144428 42-43 0.011942469752678524 44-45 0.00930014630565732 46-47 0.015770717896394292 48-49 0.01354450837803784 50-51 0.008759198198393135 52-53 0.0038490538401490023 54-55 0.004327584858113472 56-57 0.007177965269467058 58-59 0.002767157625620634 60-61 0.005700760822707171 62-63 0.005575926644107744 64-65 0.007510856412398864 66-67 0.003724219661549575 68-69 0.00620009753710488 70-71 0.017414367914620078 72-73 0.013877399520969646 74-75 0.010028345680820642 76-77 0.00744843932309915 78-79 0.005783983608440122 80-81 0.007989387430363334 82-83 0.009986734287954168 84-85 0.0042235563759472836 86-87 3.745025357982813E-4 88-89 0.023489631273125533 90-91 0.01966138312940977 92-93 0.012837114699307752 94-95 0.02415541355898914 96-97 0.018745932486347303 98-99 0.017622424878952457 100-101 0.10793995309563795 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2403188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.87864720917974 #Duplication Level Percentage of deduplicated Percentage of total 1 74.19265433010325 39.23207193834152 2 14.153587376325447 14.968451072340264 3 4.928720006803349 7.818721392977408 4 2.262251861528752 4.78499272336356 5 1.2189728491024647 3.222881762262896 6 0.7366215588779219 2.337093092314899 7 0.4692395571319251 1.7368927098719567 8 0.3426048434324923 1.4493184518418416 9 0.24026957792712517 1.1434617221676273 >10 1.3029336982671746 12.709840188615548 >50 0.10071311799286556 3.626269766649601 >100 0.04771141105963895 4.5334536195463935 >500 0.0024534926570010216 0.8560155548308478 >1k 0.0011080289418708893 0.8936393928960631 >5k 1.5828984883869848E-4 0.6868966119797257 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9118 0.3794126801565254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7295 0.30355511096094023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4522 0.1881667185422031 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.664455714659028E-4 2 0.0 0.0 0.0 0.0 1.664455714659028E-4 3 0.0 0.0 0.0 0.0 1.664455714659028E-4 4 0.0 0.0 0.0 0.0 1.664455714659028E-4 5 0.0 0.0 0.0 0.0 1.664455714659028E-4 6 0.0 0.0 0.0 0.0 2.080569643323785E-4 7 0.0 0.0 0.0 0.0 2.080569643323785E-4 8 0.0 0.0 0.0 0.0 2.080569643323785E-4 9 0.0 0.0 0.0 0.0 2.080569643323785E-4 10-11 0.0 0.0 0.0 0.0 2.080569643323785E-4 12-13 0.0 0.0 0.0 0.0 4.577253215312327E-4 14-15 0.0 0.0 0.0 2.080569643323785E-5 5.201424108309462E-4 16-17 0.0 0.0 0.0 4.16113928664757E-5 5.40948107264184E-4 18-19 0.0 0.0 0.0 4.16113928664757E-5 6.241708929971355E-4 20-21 0.0 0.0 0.0 6.241708929971355E-5 6.241708929971355E-4 22-23 0.0 0.0 0.0 1.8725126789914063E-4 6.86587982296849E-4 24-25 0.0 0.0 0.0 3.328911429318056E-4 7.073936787300868E-4 26-27 0.0 0.0 0.0 9.986734287954168E-4 8.738392501959896E-4 28-29 0.0 0.0 0.0 0.003953082322315191 8.738392501959896E-4 30-31 0.0 0.0 0.0 0.01304517166364013 9.154506430624654E-4 32-33 0.0 0.0 0.0 0.03278977757878285 9.986734287954168E-4 34-35 0.0 0.0 0.0 0.062063392460348504 0.0010402848216618924 36-37 0.0 0.0 0.0 0.10947957463169757 0.0010402848216618924 38-39 0.0 0.0 0.0 0.1740396506640346 0.0010402848216618924 40-41 0.0 0.0 0.0 0.24276086598301921 0.0010402848216618924 42-43 0.0 0.0 0.0 0.3132297598023958 0.0010402848216618924 44-45 0.0 0.0 0.0 0.4002391822861965 0.0010610905180951304 46-47 0.0 0.0 0.0 0.49950315996917427 0.001081896214528368 48-49 0.0 0.0 0.0 0.6016175180635056 0.001081896214528368 50-51 0.0 0.0 0.0 0.7105353388915058 0.0011235076073948438 52-53 0.0 0.0 0.0 0.8183920692014108 0.001248341785994271 54-55 0.0 0.0 0.0 0.9365476192457685 0.001373175964593698 56-57 0.0 4.16113928664757E-5 0.0 1.0672281985429355 0.001373175964593698 58-59 0.0 4.16113928664757E-5 0.0 1.2046498234844716 0.0014147873574601737 60-61 0.0 4.16113928664757E-5 0.0 1.350352115606436 0.0014563987503266494 62-63 0.0 4.16113928664757E-5 0.0 1.4983222286396236 0.0014563987503266494 64-65 0.0 4.16113928664757E-5 0.0 1.6474366549766395 0.001498010143193125 66-67 0.0 4.16113928664757E-5 0.0 1.8188963992829525 0.001498010143193125 68-69 0.0 4.16113928664757E-5 0.0 2.0064181412357254 0.001498010143193125 70-71 0.0 4.16113928664757E-5 0.0 2.1979137712072463 0.001498010143193125 72-73 0.0 4.16113928664757E-5 0.0 2.3889724815536697 0.0017476785003919795 74-75 0.0 4.16113928664757E-5 0.0 2.585066170436936 0.0018933183754246442 76-77 0.0 4.16113928664757E-5 0.0 2.7950164531447395 0.0019141240718578822 78-79 0.0 4.16113928664757E-5 0.0 3.0236086398567235 0.0019141240718578822 80-81 0.0 4.16113928664757E-5 0.0 3.2657245292503125 0.00193492976829112 82-83 0.0 4.16113928664757E-5 0.0 3.511252552858952 0.0020181525540240715 84-85 0.0 4.16113928664757E-5 0.0 3.7625021429867327 0.0020389582504573094 86-87 0.0 4.16113928664757E-5 0.0 4.015499411614905 0.002080569643323785 88-89 0.0 4.16113928664757E-5 0.0 4.283539198764308 0.002080569643323785 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4525 0.0 58.20893 1 GTATCAA 7740 0.0 43.46276 1 ATCAACG 9750 0.0 34.12035 3 TCAACGC 9875 0.0 33.736572 4 TATCAAC 9915 0.0 33.6484 2 CAACGCA 10085 0.0 33.0812 5 AACGCAG 10335 0.0 32.321575 6 ACGCAGA 11915 0.0 28.029125 7 CGCAGAG 11880 0.0 27.791796 8 GCAGAGT 12640 0.0 25.555418 9 GTACATG 7385 0.0 24.294416 1 TGGTATC 1755 0.0 24.100882 2 GAGTACT 8350 0.0 23.893232 12-13 TACATGG 7390 0.0 23.537304 2 GTGGTAT 1880 0.0 23.350657 1 CAGAGTA 12115 0.0 23.251333 10-11 GTACTTT 8735 0.0 22.595879 14-15 ACATGGG 7450 0.0 22.135698 3 GGACCGA 770 0.0 21.59857 6 AGAGTAC 11700 0.0 21.355831 10-11 >>END_MODULE