FastQCFastQC Report
Thu 26 May 2016
SRR938135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403188
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87530.36422452176026177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73860.3073417477117895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45530.18945667172106387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44100.054.2554051
GTATCAA74550.043.069491
ATCAACG94400.033.4013983
TCAACGC96250.032.8575334
TATCAAC96950.032.824032
CAACGCA97250.032.567895
AACGCAG100400.031.55276
TGGTATC16950.029.186182
CGCAGAG117400.026.8623738
ACGCAGA118000.026.7660357
GTGGTAT20200.025.903291
GCAGAGT128400.024.3021559
GAGTACT83550.022.34037612-13
GTACATG73600.021.9096551
CAGAGTA124200.021.51024410-11
TACATGG72200.021.478012
GTACTTT88900.020.8356614-15
AGAGTAC118200.020.83414610-11
ACATGGG74550.020.0949083
AGTACTT87250.019.59663612-13
Produced by FastQC (version 0.11.5)