FastQCFastQC Report
Thu 26 May 2016
SRR938134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382966
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89270.37461717875957945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76300.3201892095816726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45650.19156798712193124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.055.581981
GTATCAA73800.046.0586241
ATCAACG93350.036.0423433
TATCAAC94100.035.957082
TCAACGC94250.035.698174
CAACGCA95150.035.210685
AACGCAG98550.034.1355446
CGCAGAG113800.029.3885758
ACGCAGA115350.029.1584077
GCAGAGT124450.026.2628389
TGGTATC16250.026.0273952
GTACATG75350.024.5830541
TACATGG75000.024.0777782
GAGTACT82550.023.5952212-13
ACATGGG75800.023.0086383
GTGGTAT18900.022.9861851
CAGAGTA119200.022.65723610-11
GTACTTT87400.022.06868214-15
AGAGTAC115400.021.83897410-11
CATGGGG50050.021.2685574
Produced by FastQC (version 0.11.5)