FastQCFastQC Report
Thu 26 May 2016
SRR938134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382966
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85330.3580831619083109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74330.31192220115603836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46240.19404389319864404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43250.057.559021
GTATCAA72600.041.356971
ATCAACG90100.033.267343
TCAACGC92950.032.396554
TATCAAC92750.032.372142
CAACGCA93900.031.9676255
AACGCAG95900.031.3993346
TGGTATC16100.029.2130952
CGCAGAG112900.026.3768778
GTGGTAT18750.026.3511961
ACGCAGA114000.026.2056887
TAATGCG1109.485969E-525.9070195
GCAGAGT125550.023.6057429
GTACGGG2052.667548E-823.1747131
GAGTACT83900.022.33236912-13
CAGAGTA120550.022.04360610-11
GTACATG71300.021.3886681
TACATGG68800.021.0610332
GTACTTT88600.020.77245114-15
AGAGTAC117850.020.19100210-11
Produced by FastQC (version 0.11.5)