FastQCFastQC Report
Thu 26 May 2016
SRR938133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411048
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92060.38182566253347094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78520.3256675105597234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47710.19788075558844123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.054.014761
GTATCAA76000.044.807361
ATCAACG95700.035.2529833
TCAACGC96700.034.937564
TATCAAC96900.034.8654482
CAACGCA97650.034.5976645
AACGCAG102000.033.1221776
TGGTATC17150.029.6462022
ACGCAGA118250.028.5463267
CGCAGAG119050.028.0749458
GTGGTAT21100.025.3518121
GCAGAGT129550.025.3218619
GAGTACT83250.022.64064412-13
CAGAGTA123750.022.0512210-11
GTACTTT87400.021.49803714-15
GTACATG74400.021.4410951
AGAGTAC119450.021.41223710-11
GTCTACG1604.7858583E-520.895441
TACATGG73350.020.8595642
AGTACTT86550.020.81622512-13
Produced by FastQC (version 0.11.5)