##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938133_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2411048 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93329995918787 33.0 31.0 34.0 30.0 34.0 2 32.12999450861202 34.0 31.0 34.0 30.0 34.0 3 32.204996333544585 34.0 31.0 34.0 30.0 34.0 4 35.71652575975261 37.0 35.0 37.0 35.0 37.0 5 35.73473609816146 37.0 35.0 37.0 35.0 37.0 6 35.759238721087264 37.0 36.0 37.0 35.0 37.0 7 35.70277862572624 37.0 36.0 37.0 35.0 37.0 8 35.68418836953889 37.0 35.0 37.0 33.0 37.0 9 37.35779586304379 39.0 37.0 39.0 34.0 39.0 10-11 37.27677030901086 39.0 37.0 39.0 34.0 39.0 12-13 37.1862727328531 39.0 37.0 39.0 34.0 39.0 14-15 38.46638536437267 40.0 38.0 41.0 34.0 41.0 16-17 38.396786169333836 40.0 38.0 41.0 34.0 41.0 18-19 38.33028998178385 40.0 38.0 41.0 34.0 41.0 20-21 38.22250282864547 40.0 38.0 41.0 33.5 41.0 22-23 38.077322392586126 40.0 38.0 41.0 33.0 41.0 24-25 38.03729208211533 40.0 38.0 41.0 33.0 41.0 26-27 38.05832401511708 40.0 38.0 41.0 33.0 41.0 28-29 37.97377074201758 40.0 38.0 41.0 33.0 41.0 30-31 37.79804466771296 40.0 38.0 41.0 33.0 41.0 32-33 37.58722140745435 40.0 37.0 41.0 32.5 41.0 34-35 37.394308408625626 40.0 37.0 41.0 32.0 41.0 36-37 37.1899690508028 40.0 37.0 41.0 31.0 41.0 38-39 36.9607649868439 40.0 36.5 41.0 30.5 41.0 40-41 36.74155056224513 40.0 36.0 41.0 30.0 41.0 42-43 36.466898419276596 39.0 36.0 41.0 30.0 41.0 44-45 36.25932893082178 39.0 35.5 41.0 30.0 41.0 46-47 36.13895554961992 39.0 36.0 41.0 29.0 41.0 48-49 35.90406350267601 39.0 35.0 41.0 28.0 41.0 50-51 35.2655370195865 38.5 34.5 40.5 26.5 40.5 52-53 35.33282020930318 39.0 35.0 40.0 27.0 41.0 54-55 35.48297275707493 39.0 35.0 41.0 27.0 41.0 56-57 35.2777866720198 39.0 35.0 41.0 26.5 41.0 58-59 35.03092804456817 38.5 35.0 40.0 26.0 41.0 60-61 34.66697054558847 38.0 34.0 40.0 26.0 41.0 62-63 34.251210676850896 37.5 34.0 40.0 24.5 41.0 64-65 33.82634750531719 37.0 34.0 40.0 23.0 41.0 66-67 33.40485112697881 36.5 33.0 39.0 22.0 41.0 68-69 32.92170728247633 36.0 33.0 39.0 20.5 41.0 70-71 32.43887492078134 35.0 32.0 39.0 19.5 40.0 72-73 31.952629313062204 35.0 32.0 38.0 17.5 40.0 74-75 31.425429108006142 35.0 31.0 37.0 12.5 39.0 76-77 30.88409874046473 35.0 31.0 36.5 7.5 39.0 78-79 30.333859591347828 34.5 30.0 36.0 6.5 38.5 80-81 29.81728464136757 34.0 29.5 35.5 2.0 37.0 82-83 29.290980312295734 34.0 29.0 35.0 2.0 37.0 84-85 28.80388175598329 34.0 29.0 35.0 2.0 36.5 86-87 28.311353817924818 34.0 28.0 35.0 2.0 36.0 88-89 27.778875824952472 33.5 26.5 35.0 2.0 35.5 90-91 27.190372817131802 33.0 26.0 35.0 2.0 35.0 92-93 26.586618972330704 33.0 25.0 35.0 2.0 35.0 94-95 25.997448827231977 32.5 24.0 35.0 2.0 35.0 96-97 25.17651784618141 32.0 19.0 34.5 2.0 35.0 98-99 24.123688951858277 31.5 4.5 34.0 2.0 35.0 100-101 21.235727783105105 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2324781676190497 1101 2 0.11573444859752158 1101 3 0.15178393437412296 1101 4 0.05865304542197691 1101 5 -0.0038243312545347408 1101 6 0.04042794973088348 1101 7 0.13477598350115017 1101 8 0.06896054018825737 1101 9 0.05889573019755545 1101 10-11 0.05870803358209997 1101 12-13 0.04546660410745318 1101 14-15 0.05687526749213134 1101 16-17 0.08981356979003152 1101 18-19 0.10269587314623863 1101 20-21 0.20823104471933362 1101 22-23 0.11380386898952821 1101 24-25 0.04167903688513519 1101 26-27 0.09821223402955326 1101 28-29 0.10485878641761559 1101 30-31 0.22931255341342904 1101 32-33 0.19268761977206594 1101 34-35 0.2628345494801607 1101 36-37 0.2848211907499163 1101 38-39 0.3131077149743504 1101 40-41 0.3398001301748792 1101 42-43 0.29917942495781347 1101 44-45 0.4013030925068577 1101 46-47 0.44432032467143756 1101 48-49 0.5238251244633503 1101 50-51 0.6013697362527779 1101 52-53 0.6885667945226217 1101 54-55 0.5627797575175251 1101 56-57 0.5780276936136133 1101 58-59 0.53355152361069 1101 60-61 0.4365919756629211 1101 62-63 0.42193427854765986 1101 64-65 0.4881451099839538 1101 66-67 0.3900792603645016 1101 68-69 0.4525119962309745 1101 70-71 0.559395214410614 1101 72-73 0.6603148829981045 1101 74-75 0.6805387534948792 1101 76-77 0.7486816636114852 1101 78-79 0.7303789622149175 1101 80-81 0.8576111147384857 1101 82-83 0.8433521870548439 1101 84-85 0.8908993839809121 1101 86-87 0.973515744704013 1101 88-89 1.0248545717201196 1101 90-91 1.0204073884280085 1101 92-93 1.0845694357974907 1101 94-95 1.095371506334832 1101 96-97 1.1978529548440875 1101 98-99 1.254913040771335 1101 100-101 0.9635778098514969 1102 1 0.32412699091344166 1102 2 0.2919010462831082 1102 3 0.21985151509993273 1102 4 0.17646093031846988 1102 5 0.10075617206014442 1102 6 0.28792154405991965 1102 7 0.12387737133671095 1102 8 0.2024750585129027 1102 9 0.34888326575471496 1102 10-11 0.32557378842253826 1102 12-13 0.15843979239709682 1102 14-15 0.230840012975861 1102 16-17 0.2439829378834517 1102 18-19 0.2415174689298567 1102 20-21 0.25719608925400195 1102 22-23 0.27891347713711667 1102 24-25 0.2721489774816632 1102 26-27 0.24612689207079796 1102 28-29 0.26242573195924734 1102 30-31 0.2583538678977817 1102 32-33 0.3625551282471733 1102 34-35 0.1865161535415183 1102 36-37 0.338937327777451 1102 38-39 0.19586718950597515 1102 40-41 0.22307660395768636 1102 42-43 0.23668280423334664 1102 44-45 0.26866204214428535 1102 46-47 0.1971605908028451 1102 48-49 0.22819234465335114 1102 50-51 0.31376731130858815 1102 52-53 0.3316662540777813 1102 54-55 0.39098205470716607 1102 56-57 0.40215823293402764 1102 58-59 0.24644217384311418 1102 60-61 0.22423578925609888 1102 62-63 0.39759117382480014 1102 64-65 0.2880144845368733 1102 66-67 0.09939338107102458 1102 68-69 0.27813288577591067 1102 70-71 0.14090728769483007 1102 72-73 0.3254919570359327 1102 74-75 0.31891380642689526 1102 76-77 0.13564639234836307 1102 78-79 0.08851853048107117 1102 80-81 0.1636558910364272 1102 82-83 0.2264700442233334 1102 84-85 0.3054723751361834 1102 86-87 0.2840845635006808 1102 88-89 0.2949212481159087 1102 90-91 0.3825025631814114 1102 92-93 0.40977249138029137 1102 94-95 0.24417757539406537 1102 96-97 0.43462557326227724 1102 98-99 0.6163959984568237 1102 100-101 0.7490417813914156 1103 1 0.3087170726282551 1103 2 0.2690753448618537 1103 3 0.23187423348994685 1103 4 0.11346529280962869 1103 5 0.14205434641068848 1103 6 0.2511794732434254 1103 7 0.26215930438795 1103 8 0.3101538110799069 1103 9 0.3332180592762626 1103 10-11 0.38014096197558445 1103 12-13 0.17132156685337208 1103 14-15 0.35342748306986493 1103 16-17 0.2614502982713063 1103 18-19 0.39969787409255275 1103 20-21 0.473951014847664 1103 22-23 0.4348862042250019 1103 24-25 0.4071749694344362 1103 26-27 0.24565404411637104 1103 28-29 0.4118706495779705 1103 30-31 0.49635622648958844 1103 32-33 0.49246643254391387 1103 34-35 0.5891757820859027 1103 36-37 0.5979618783685652 1103 38-39 0.5842410714905384 1103 40-41 0.6659201755524649 1103 42-43 0.4736288796856982 1103 44-45 0.5277931134284373 1103 46-47 0.5825195870006254 1103 48-49 0.5722542960943784 1103 50-51 0.4232467493130869 1103 52-53 0.7174385686380162 1103 54-55 0.7237225395739983 1103 56-57 0.5132015935206979 1103 58-59 0.6101735157779231 1103 60-61 0.6714301042365491 1103 62-63 0.6062647596298802 1103 64-65 0.6412847714762862 1103 66-67 0.6418986393300514 1103 68-69 0.7358197714181003 1103 70-71 0.8069003022869268 1103 72-73 0.677150765495238 1103 74-75 0.6761104483705225 1103 76-77 0.7117679095283549 1103 78-79 0.6587399534529403 1103 80-81 0.7030725010351411 1103 82-83 0.5713870981523996 1103 84-85 0.6139290789363621 1103 86-87 0.7871438423228305 1103 88-89 0.7330493304949215 1103 90-91 0.9152106287068875 1103 92-93 0.9564353324893737 1103 94-95 1.11160596479667 1103 96-97 1.0429797099476446 1103 98-99 1.2836422973428192 1103 100-101 1.4477800251442012 1104 1 0.38160183674271764 1104 2 0.3102591551222318 1104 3 0.24007222266241257 1104 4 0.19687854364747892 1104 5 0.18658025515005505 1104 6 0.24484072614365715 1104 7 0.23814589887106763 1104 8 0.21116747439519656 1104 9 0.1666008899027176 1104 10-11 0.3271077059817742 1104 12-13 0.13944531008615968 1104 14-15 0.21651436113121747 1104 16-17 0.37656759654405647 1104 18-19 0.3812456956960659 1104 20-21 0.39422995271824846 1104 22-23 0.3829630281486871 1104 24-25 0.4165990478051498 1104 26-27 0.41479918461650556 1104 28-29 0.4114468290056621 1104 30-31 0.3976385157393949 1104 32-33 0.48071546579991065 1104 34-35 0.3814902961359081 1104 36-37 0.38130603923476514 1104 38-39 0.32321090907754524 1104 40-41 0.5942615006362573 1104 42-43 0.5323342161126092 1104 44-45 0.5743530515568196 1104 46-47 0.5283154106665222 1104 48-49 0.48303233167055737 1104 50-51 0.5506888735719144 1104 52-53 0.4033890713448969 1104 54-55 0.40876610750387954 1104 56-57 0.358081201667126 1104 58-59 0.5548881329472977 1104 60-61 0.5855600346309799 1104 62-63 0.5527025013158848 1104 64-65 0.5402226420614866 1104 66-67 0.5764467187319582 1104 68-69 0.653025236744206 1104 70-71 0.6585074175228129 1104 72-73 0.6092703839536036 1104 74-75 0.7559026628587873 1104 76-77 0.7689388298686524 1104 78-79 0.810028429864385 1104 80-81 0.7789662880936596 1104 82-83 0.7755902432928998 1104 84-85 0.8732521003336302 1104 86-87 0.7363647755530458 1104 88-89 0.9265187813843276 1104 90-91 0.9910822451028665 1104 92-93 0.7955500527781076 1104 94-95 0.9383597673230959 1104 96-97 0.9280320430231797 1104 98-99 1.107731495589789 1104 100-101 1.1758759261496117 1105 1 0.35850259790888117 1105 2 0.3029044708386337 1105 3 0.23742968352698313 1105 4 0.20853252421140667 1105 5 0.22598108812434958 1105 6 0.2138247847390886 1105 7 0.21635778072275968 1105 8 0.25327997379679346 1105 9 0.3058046813719102 1105 10-11 0.374637793587695 1105 12-13 0.1999014353195392 1105 14-15 0.25476061514050485 1105 16-17 0.35346740026850654 1105 18-19 0.29210863791445263 1105 20-21 0.3623211969137543 1105 22-23 0.17812842620508462 1105 24-25 0.21430616638430422 1105 26-27 0.16566452043918645 1105 28-29 0.36531269142128764 1105 30-31 0.40561237758055313 1105 32-33 0.4106097642345006 1105 34-35 0.3918446453149045 1105 36-37 0.4320853010472021 1105 38-39 0.4544319344976202 1105 40-41 0.38423344759397793 1105 42-43 0.4677146555783551 1105 44-45 0.4860172767471127 1105 46-47 0.4836975735273583 1105 48-49 0.3997814340689487 1105 50-51 0.29786355881295634 1105 52-53 0.3354163584337968 1105 54-55 0.37938490208475173 1105 56-57 0.5828019637907289 1105 58-59 0.5357360433307505 1105 60-61 0.4752239725887577 1105 62-63 0.4026660227865122 1105 64-65 0.5872962214573434 1105 66-67 0.6329265885198794 1105 68-69 0.47275140841350805 1105 70-71 0.6666148424833125 1105 72-73 0.5896393819908461 1105 74-75 0.5485651167819015 1105 76-77 0.5464913216107163 1105 78-79 0.6854332840938859 1105 80-81 0.7570230643874041 1105 82-83 0.701892890619245 1105 84-85 0.5945755720172912 1105 86-87 0.7760915216873734 1105 88-89 0.6219667204910344 1105 90-91 0.41910436200650025 1105 92-93 0.7212637777051008 1105 94-95 0.830858307224478 1105 96-97 0.8734273228355178 1105 98-99 0.9209144148295785 1105 100-101 0.9837392034726058 1106 1 0.048374901281427185 1106 2 0.056157143829118183 1106 3 0.05605990290880669 1106 4 0.098733039890881 1106 5 0.10350983655842327 1106 6 0.07866348968838821 1106 7 0.19676963306304884 1106 8 0.09172037000394795 1106 9 0.0990712804949041 1106 10-11 -0.4973117256296007 1106 12-13 -0.2439907811920179 1106 14-15 -0.0915594640589461 1106 16-17 0.11055238351498531 1106 18-19 0.19830556783195163 1106 20-21 0.13828417830040252 1106 22-23 -0.057547028316847104 1106 24-25 0.1939215546883446 1106 26-27 0.10343075865169027 1106 28-29 0.022359330755627127 1106 30-31 0.1412955225992576 1106 32-33 0.1721327974532798 1106 34-35 -0.18190920920731912 1106 36-37 -0.20417121122022053 1106 38-39 0.039760258090566936 1106 40-41 0.08907525769443936 1106 42-43 -0.026045456293950053 1106 44-45 0.028209694423956932 1106 46-47 0.24421713263208034 1106 48-49 0.27394147348772435 1106 50-51 0.2585031439531775 1106 52-53 0.07493688038871937 1106 54-55 -0.10290424575572388 1106 56-57 -0.040732928716941785 1106 58-59 0.03045638158231867 1106 60-61 -0.20968986037111392 1106 62-63 -0.08430491853572164 1106 64-65 0.02883713643826269 1106 66-67 -0.18216600234696045 1106 68-69 -0.6259257175700341 1106 70-71 -0.3605781207664194 1106 72-73 -0.009213539701260487 1106 74-75 0.021117371897943826 1106 76-77 -0.005752756299624906 1106 78-79 0.10133236378570842 1106 80-81 0.16851820372201232 1106 82-83 0.3170525607132184 1106 84-85 0.05828574632737471 1106 86-87 -0.02251002028437199 1106 88-89 -0.01925414315718399 1106 90-91 0.04026298148813723 1106 92-93 0.23658668537878924 1106 94-95 0.13928477548959606 1106 96-97 0.06288117270725024 1106 98-99 -0.08832720990276854 1106 100-101 -0.27297301687152853 1107 1 0.16971254673068614 1107 2 0.10481029832319422 1107 3 0.17051746935314327 1107 4 0.04456406310195149 1107 5 0.08919878703934359 1107 6 0.12779138799685086 1107 7 0.06699307095289697 1107 8 0.06381729388977675 1107 9 0.07427678240171076 1107 10-11 0.10812334978442095 1107 12-13 -0.023685054935903338 1107 14-15 0.10466598567995788 1107 16-17 0.1782202239006594 1107 18-19 0.17185620512245237 1107 20-21 0.16163709271022242 1107 22-23 0.2613304569493309 1107 24-25 0.38264317135107717 1107 26-27 0.3355621208379276 1107 28-29 0.34800840140838574 1107 30-31 0.4354812754666355 1107 32-33 0.3443713021416741 1107 34-35 0.42425162786474857 1107 36-37 0.4365150243932092 1107 38-39 0.5128266331048223 1107 40-41 0.47748973970564634 1107 42-43 0.5382684319168547 1107 44-45 0.4211232079948459 1107 46-47 0.4584742554318595 1107 48-49 0.34294568907127854 1107 50-51 0.4004217255574716 1107 52-53 0.43851382064293176 1107 54-55 0.566556721222284 1107 56-57 0.4599279726258061 1107 58-59 0.38733257739173865 1107 60-61 0.494115991309684 1107 62-63 0.4632745029203704 1107 64-65 0.47116528145178194 1107 66-67 0.36589557553099183 1107 68-69 0.45752583842892847 1107 70-71 0.5363943007959762 1107 72-73 0.5731837413543239 1107 74-75 0.579072337949043 1107 76-77 0.6171048948505984 1107 78-79 0.379656989832295 1107 80-81 0.40970676141897044 1107 82-83 0.4794538614236643 1107 84-85 0.5540631713324231 1107 86-87 0.5122669015057113 1107 88-89 0.6487987653394001 1107 90-91 0.6165928748662033 1107 92-93 0.611939142228568 1107 94-95 0.5924608503555859 1107 96-97 0.6846281120163589 1107 98-99 0.8335983227057149 1107 100-101 0.887376928957476 1108 1 0.23126398093000233 1108 2 0.17847978117525543 1108 3 0.11059629821441774 1108 4 0.13191623097566918 1108 5 0.0684807606036344 1108 6 0.1458111970135718 1108 7 0.1015780173422911 1108 8 0.05582332258176592 1108 9 0.09864877497853541 1108 10-11 0.1522835506213056 1108 12-13 0.06211304086561853 1108 14-15 0.16694603622211446 1108 16-17 0.2075980124348078 1108 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-0.7658876321994867 2316 60-61 -0.6198053619961286 2316 62-63 -0.5815727433228872 2316 64-65 -0.6150504511441497 2316 66-67 -0.7755209403699936 2316 68-69 -0.6220937860887403 2316 70-71 -0.6760101756481234 2316 72-73 -0.7551915495601733 2316 74-75 -0.7020676334837752 2316 76-77 -0.6020297314471961 2316 78-79 -0.5553965128797316 2316 80-81 -0.556992626473626 2316 82-83 -0.7104069806643487 2316 84-85 -0.5952289878751316 2316 86-87 -0.609827743011742 2316 88-89 -0.8032596904041363 2316 90-91 -0.7250206072810599 2316 92-93 -0.6041928856631458 2316 94-95 -0.7699293575294277 2316 96-97 -1.0068508773738003 2316 98-99 -0.9761488569676402 2316 100-101 -0.8272966743084318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6048.0 3 1603.0 4 1658.0 5 1856.0 6 2222.0 7 2662.0 8 3224.0 9 4077.0 10 5260.0 11 6528.0 12 8214.0 13 10293.0 14 12175.0 15 14465.0 16 17427.0 17 23630.0 18 21353.0 19 16860.0 20 16233.0 21 17200.0 22 18558.0 23 20712.0 24 22538.0 25 25472.0 26 28888.0 27 33512.0 28 39227.0 29 46166.0 30 55847.0 31 68218.0 32 84978.0 33 111388.0 34 152551.0 35 224790.0 36 345226.0 37 495331.0 38 395466.0 39 49187.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25183589130506 18.518231358361565 14.805597900099668 23.424334850233706 2 15.417221675858034 19.600642358301485 40.57650412677862 24.405631839061858 3 19.203988192784784 23.539359251495924 29.678679577194462 27.57797297852483 4 12.83306564405211 16.571543591850627 36.7244377487539 33.87095301534337 5 13.966394647052475 36.85684518126801 34.33017591339648 14.846584258283038 6 32.74651469171054 35.63258288363511 17.17773172727714 14.44317069737721 7 28.82966227966415 32.52254720074376 20.261601484193097 18.386189035399 8 26.257951349711657 35.102047421976245 19.56630623729413 19.073694991017966 9 26.180015121323308 16.95945211345806 20.17700745526055 36.68352530995808 10-11 25.04425052406442 26.411456304514164 27.046351969986222 21.497941201435193 12-13 25.814543989267918 23.798437977155295 27.4897295160702 22.897288517506585 14-15 22.848932148422342 25.258336462986748 25.724962599777157 26.167768788813756 16-17 22.304293442805278 28.364793237794384 26.660194021649815 22.670719297750523 18-19 22.238561647346053 26.90055445915177 28.881800248210787 21.979083645291393 20-21 22.739321769032898 26.04574183392774 29.280958127575325 21.933978269464042 22-23 22.647047593593097 25.856849023417883 29.0924854099935 22.40361797299552 24-25 22.383974468343013 26.204590334882983 29.089998841809916 22.32143635496409 26-27 22.08105958373911 26.43624159541842 28.986486472465263 22.49621234837721 28-29 22.23365167483099 26.702274818455702 28.90767958224786 22.156393924465455 30-31 22.275917455943024 26.388967286189807 28.90419942059783 22.43091583726934 32-33 22.071242542153048 26.29395590142672 28.933271076523997 22.70153047989624 34-35 22.67413373864598 26.436522797694884 28.684717163481103 22.204626300178038 36-37 22.675641626109254 26.062895410124614 28.742683220136428 22.518779743629704 38-39 22.215731016802838 26.48860617107558 28.69108876670393 22.604574045417646 40-41 22.72843548711348 26.312163163892727 28.607581228110906 22.35182012088289 42-43 22.334884263230844 26.225037182691963 28.539108681756613 22.90096987232058 44-45 22.40558993224777 26.52583561912063 28.596962184532316 22.471612264099285 46-47 22.663120342285854 26.738489277430183 28.014199403165392 22.584190977118574 48-49 22.510892464347975 26.66320110256383 28.095359988582725 22.730546444505475 50-51 22.68210368231526 26.951925789482416 27.90751895919417 22.458451569008147 52-53 22.774646856018233 26.77551059354159 27.793845517515475 22.655997032924706 54-55 22.61589198605332 26.85822433126534 27.823825803013648 22.70205787966769 56-57 22.606459936796625 27.027149241486885 27.822636433313384 22.5437543884031 58-59 22.659633098122804 26.642897397694416 27.81711781469304 22.880351689489743 60-61 22.539743914728408 26.909780203777927 27.863493100019127 22.686982781474537 62-63 22.43987733306231 26.86992928087182 28.11404250199899 22.576150884066884 64-65 22.33599816140553 26.822342905336033 27.951323709198405 22.89033522406003 66-67 22.361772461806435 26.86145533150176 27.826867179854986 22.949905026836813 68-69 22.47923866604774 26.657868067473352 28.060831880382192 22.80206138609672 70-71 22.441939807810044 26.63411150785031 27.845448885475427 23.07849979886422 72-73 22.45602250147605 26.678679735269917 27.691271052086737 23.1740267111673 74-75 22.40861987919291 27.08727044765171 27.548190279256968 22.95591939389841 76-77 22.537998056849194 26.83379671921139 27.568618031477055 23.059587192462367 78-79 22.556024504829182 26.805833222888626 27.63401298829784 23.004129283984355 80-81 22.601203030786362 26.93629943482318 27.470260756228114 22.992236778162344 82-83 22.587492771335242 26.99247721051946 27.38415260966206 23.035877408483238 84-85 22.6106855757442 26.93688997887891 27.411891570790708 23.04053287458618 86-87 22.72441441329335 26.90368047518904 27.32845119730549 23.043453914212115 88-89 22.686690155615842 26.900095619649143 27.199028907672577 23.214185317062434 90-91 22.645467713252255 26.869399571625003 27.213659863279364 23.271472851843374 92-93 22.64211729512975 26.594661538557286 27.401322272217136 23.361898894095827 94-95 22.529236511201304 26.626238465523812 27.418134345096547 23.426390678178336 96-97 22.777673237100124 26.43126272944114 27.26905886626776 23.522005167190972 98-99 22.533319116291942 26.548416286589 27.144121384767487 23.774143212351568 100-101 23.012330404486626 25.905066812021886 27.333476805137728 23.74912597835376 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 666.0 1 444.5 2 320.0 3 423.0 4 361.0 5 367.5 6 681.5 7 1459.5 8 2506.0 9 3034.0 10 2691.5 11 2309.0 12 2322.5 13 2450.0 14 2588.5 15 2674.5 16 2795.5 17 2950.0 18 3145.0 19 3416.0 20 3893.5 21 4783.0 22 5811.0 23 6963.0 24 8649.5 25 11209.0 26 14323.0 27 17410.5 28 19831.0 29 22267.5 30 25757.5 31 30448.5 32 36153.0 33 39714.0 34 42184.0 35 46198.0 36 51305.5 37 56888.5 38 60752.5 39 63584.5 40 67054.0 41 71849.5 42 75257.5 43 77188.0 44 80290.5 45 84168.5 46 87222.0 47 89773.5 48 90172.0 49 89123.5 50 89451.5 51 88375.5 52 87188.0 53 88344.5 54 89851.0 55 84804.0 56 76369.5 57 69738.0 58 64031.0 59 56470.0 60 45273.0 61 36411.0 62 29715.5 63 22478.5 64 16899.5 65 13016.5 66 9525.5 67 7138.0 68 5392.0 69 3851.5 70 2831.5 71 1944.0 72 1298.5 73 1004.5 74 751.5 75 527.5 76 334.5 77 187.0 78 117.0 79 62.5 80 34.5 81 27.5 82 20.5 83 16.5 84 13.5 85 9.5 86 9.5 87 6.0 88 4.0 89 2.5 90 3.0 91 5.0 92 4.0 93 3.5 94 2.5 95 1.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5009025121026209 2 0.04885842173196054 3 0.0553701129135546 4 0.06378968813561571 5 0.07913571193937242 6 0.10704888496620557 7 0.06876677693683411 8 0.051305490392559584 9 0.05001974245224483 10-11 0.0727691858478139 12-13 0.10653043821607865 14-15 0.054042889233229696 16-17 0.05889555081441763 18-19 0.05816972536423994 20-21 0.07322541898792559 22-23 0.06312607629545326 24-25 0.08780414160149445 26-27 0.06526207690597616 28-29 0.0655731449560523 30-31 0.06918153433693564 32-33 0.06938891303698641 34-35 0.07111015624740777 36-37 0.06650634910628075 38-39 0.039381215139640526 40-41 0.042699274340452786 42-43 0.0409987690000365 44-45 0.020737870005076632 46-47 0.02758136710675192 48-49 0.027622842846762072 50-51 0.025217249926173184 52-53 0.02461585169602596 54-55 0.02411814281590412 56-57 0.0238692883758432 58-59 0.021256316755203546 60-61 0.028162027466894064 62-63 0.01889219957462481 64-65 0.021194103145188315 66-67 0.015221596583726247 68-69 0.018124898384436976 70-71 0.040832866039995884 72-73 0.03712078730908717 74-75 0.031480086667706325 76-77 0.02198214220538123 78-79 0.014081013733447032 80-81 0.016030373513924237 82-83 0.02098672444513755 84-85 0.01760645163431006 86-87 0.009041711322213411 88-89 0.040584011599934966 90-91 0.05157508270262558 92-93 0.03330501922815307 94-95 0.05232164602280834 96-97 0.04290665304050355 98-99 0.03297321330807185 100-101 0.16884773758133392 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2411048.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.8102591499283 #Duplication Level Percentage of deduplicated Percentage of total 1 74.23253160972986 39.20239231665077 2 14.101940292772621 14.894542427562754 3 4.920016544184562 7.79482046160964 4 2.263565550109041 4.781579332164338 5 1.23195070320528 3.252981794810361 6 0.7294702276331002 2.311410705807672 7 0.5044739424703066 1.8648979745370036 8 0.3205275918418323 1.3541716151895686 9 0.24032790976394192 1.1422601276034918 >10 1.3033089470403918 12.660665158154055 >50 0.09939632366396645 3.6003029424079984 >100 0.04862748475083019 4.613173010450622 >500 0.002680360742124407 0.9436425477035076 >1k 0.0010248438131644836 0.8729921725396572 >5k 1.5766827894838208E-4 0.7101674128085017 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9206 0.38182566253347094 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7852 0.3256675105597234 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4771 0.19788075558844123 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.147574001015326E-5 7 0.0 0.0 0.0 4.147574001015326E-5 8.295148002030652E-5 8 0.0 0.0 0.0 4.147574001015326E-5 8.295148002030652E-5 9 0.0 0.0 0.0 4.147574001015326E-5 8.295148002030652E-5 10-11 0.0 0.0 0.0 8.295148002030652E-5 1.244272200304598E-4 12-13 0.0 0.0 0.0 8.295148002030652E-5 3.1106805007614946E-4 14-15 0.0 0.0 0.0 8.295148002030652E-5 4.769710101167625E-4 16-17 0.0 0.0 0.0 8.295148002030652E-5 5.806603601421457E-4 18-19 0.0 0.0 0.0 1.0368935002538315E-4 7.050875801726055E-4 20-21 0.0 0.0 0.0 1.4516509003553642E-4 7.050875801726055E-4 22-23 0.0 0.0 0.0 2.2811657005584294E-4 7.465633201827587E-4 24-25 0.0 0.0 0.0 3.94019530096456E-4 7.88039060192912E-4 26-27 2.073787000507663E-5 0.0 0.0 0.0010368935002538315 8.295148002030652E-4 28-29 4.147574001015326E-5 0.0 0.0 0.004043884650989943 8.295148002030652E-4 30-31 4.147574001015326E-5 0.0 0.0 0.013873635033396265 8.502526702081418E-4 32-33 4.147574001015326E-5 0.0 0.0 0.03347092218819368 9.124662802233718E-4 34-35 4.147574001015326E-5 0.0 0.0 0.061964755575168964 9.124662802233718E-4 36-37 4.147574001015326E-5 0.0 0.0 0.10765028319635278 9.53942020233525E-4 38-39 4.147574001015326E-5 0.0 0.0 0.17291236010232897 9.746798902386016E-4 40-41 4.147574001015326E-5 0.0 0.0 0.24238422461933565 9.954177602436783E-4 42-43 4.147574001015326E-5 0.0 0.0 0.3144897986269871 0.0010368935002538315 44-45 4.147574001015326E-5 0.0 0.0 0.4026257461485628 0.001119844980274138 46-47 4.147574001015326E-5 0.0 0.0 0.5048841831435956 0.0012027964602944446 48-49 4.147574001015326E-5 0.0 0.0 0.6100251840693341 0.001285747940314751 50-51 4.147574001015326E-5 0.0 0.0 0.7170325932955296 0.001410175160345211 52-53 4.147574001015326E-5 0.0 0.0 0.821592933861126 0.001513864510370594 54-55 4.147574001015326E-5 0.0 0.0 0.9373517242294638 0.0015968159903909005 56-57 4.147574001015326E-5 0.0 0.0 1.0701363058719693 0.001638291730401054 58-59 4.147574001015326E-5 0.0 0.0 1.2096192195261148 0.0016590296004061304 60-61 4.147574001015326E-5 0.0 0.0 1.3558834166719202 0.0016590296004061304 62-63 4.147574001015326E-5 0.0 0.0 1.5001360404272333 0.0017005053404162836 64-65 4.147574001015326E-5 0.0 0.0 1.652974142364648 0.0017005053404162836 66-67 4.147574001015326E-5 0.0 0.0 1.8301585036880228 0.0017005053404162836 68-69 4.147574001015326E-5 0.0 0.0 2.018624266294159 0.0017005053404162836 70-71 4.147574001015326E-5 0.0 0.0 2.2145971378421336 0.0017212432104213604 72-73 4.147574001015326E-5 0.0 0.0 2.41527750588126 0.0019493597804772032 74-75 4.147574001015326E-5 0.0 0.0 2.614381795800001 0.0020530491305025865 76-77 4.147574001015326E-5 0.0 0.0 2.8324819746433914 0.0020945248705127395 78-79 4.147574001015326E-5 0.0 0.0 3.070200178511585 0.0021152627405178165 80-81 4.147574001015326E-5 0.0 0.0 3.308146498949834 0.002198214220538123 82-83 4.147574001015326E-5 0.0 0.0 3.5608374449616926 0.002405592920588889 84-85 4.147574001015326E-5 0.0 0.0 3.820081557895156 0.0024263307905939657 86-87 4.147574001015326E-5 0.0 0.0 4.085381128870102 0.0025300201406193486 88-89 4.147574001015326E-5 0.0 0.0 4.375669833201163 0.002571495880629502 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4315 0.0 54.01476 1 GTATCAA 7600 0.0 44.80736 1 ATCAACG 9570 0.0 35.252983 3 TCAACGC 9670 0.0 34.93756 4 TATCAAC 9690 0.0 34.865448 2 CAACGCA 9765 0.0 34.597664 5 AACGCAG 10200 0.0 33.122177 6 TGGTATC 1715 0.0 29.646202 2 ACGCAGA 11825 0.0 28.546326 7 CGCAGAG 11905 0.0 28.074945 8 GTGGTAT 2110 0.0 25.351812 1 GCAGAGT 12955 0.0 25.321861 9 GAGTACT 8325 0.0 22.640644 12-13 CAGAGTA 12375 0.0 22.05122 10-11 GTACTTT 8740 0.0 21.498037 14-15 GTACATG 7440 0.0 21.441095 1 AGAGTAC 11945 0.0 21.412237 10-11 GTCTACG 160 4.7858583E-5 20.89544 1 TACATGG 7335 0.0 20.859564 2 AGTACTT 8655 0.0 20.816225 12-13 >>END_MODULE