FastQCFastQC Report
Thu 26 May 2016
SRR938133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411048
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90770.3764752920721611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78420.3252527531596219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46810.1941479389875274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43650.055.5095861
GTATCAA75000.044.088981
ATCAACG94850.034.7046663
CCGATCG1107.023482E-834.542469
TATCAAC96050.034.377122
TCAACGC96200.034.3135534
CAACGCA97600.033.8700185
AACGCAG101050.032.7136426
CGCAGAG119200.027.4535778
ACGCAGA120350.027.2701767
TGGTATC17100.026.3942642
GCAGAGT131950.024.5488389
GTGGTAT19500.024.1202981
GAGTACT83600.023.20821412-13
GTGTAAG9500.023.0046841
CAGAGTA126050.021.77915210-11
GTACTTT89200.021.56483514-15
GTACATG73500.021.0076791
TACATGG70800.020.9364682
AGAGTAC121150.020.50379410-11
Produced by FastQC (version 0.11.5)