##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938133_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2411048 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.542497702244006 34.0 31.0 34.0 31.0 34.0 2 32.76074138714783 34.0 31.0 34.0 31.0 34.0 3 32.822898175399246 34.0 31.0 34.0 31.0 34.0 4 36.26622116191797 37.0 37.0 37.0 35.0 37.0 5 36.22812859802045 37.0 37.0 37.0 35.0 37.0 6 36.259862930974414 37.0 37.0 37.0 35.0 37.0 7 36.22855745717215 37.0 37.0 37.0 35.0 37.0 8 36.21931790657009 37.0 37.0 37.0 35.0 37.0 9 37.93862337041818 39.0 38.0 39.0 35.0 39.0 10-11 37.928241577936234 39.0 38.0 39.0 35.0 39.0 12-13 37.8798926856703 39.0 38.0 39.0 35.0 39.0 14-15 39.223071461041 40.5 39.0 41.0 36.0 41.0 16-17 39.17206376646172 40.0 39.0 41.0 36.0 41.0 18-19 39.19230994156898 40.0 39.0 41.0 36.0 41.0 20-21 39.11745307434775 40.0 39.0 41.0 36.0 41.0 22-23 38.9828329838311 40.0 38.5 41.0 35.0 41.0 24-25 38.9542707154731 40.0 38.5 41.0 35.0 41.0 26-27 38.92631606670626 40.0 38.0 41.0 35.0 41.0 28-29 38.87382913985951 40.0 38.0 41.0 35.0 41.0 30-31 38.69819265315331 40.0 38.0 41.0 35.0 41.0 32-33 38.54104605134365 40.0 38.0 41.0 34.5 41.0 34-35 38.3570080313623 40.0 38.0 41.0 34.0 41.0 36-37 38.24082494417365 40.0 38.0 41.0 34.0 41.0 38-39 38.24160095526925 40.0 38.0 41.0 34.0 41.0 40-41 38.19448472199641 40.0 38.0 41.0 34.0 41.0 42-43 38.05378698391736 40.0 38.0 41.0 33.5 41.0 44-45 37.865601389935 40.0 38.0 41.0 33.0 41.0 46-47 37.566990785749596 40.0 37.0 41.0 33.0 41.0 48-49 37.25514921312226 40.0 37.0 41.0 32.0 41.0 50-51 36.97772420955535 40.0 37.0 41.0 31.0 41.0 52-53 36.58299668857691 39.5 36.0 41.0 30.5 41.0 54-55 36.37551492131223 39.0 35.5 41.0 30.0 41.0 56-57 36.14007539460019 39.0 35.0 41.0 30.0 41.0 58-59 35.82090816939356 39.0 35.0 40.0 29.0 41.0 60-61 35.47961529592111 38.0 35.0 40.0 28.0 41.0 62-63 35.12851382469366 38.0 34.5 40.0 28.0 41.0 64-65 34.74503245061898 37.0 34.0 40.0 27.5 41.0 66-67 34.33472767858624 37.0 34.0 39.5 26.5 41.0 68-69 33.88329431848723 36.0 33.5 39.0 26.0 40.5 70-71 33.377126253811625 35.5 33.0 39.0 25.5 40.0 72-73 32.96288895948982 35.0 33.0 38.0 25.0 40.0 74-75 32.513067761405 35.0 32.0 37.0 24.0 39.0 76-77 31.19207809218232 34.0 30.5 36.0 22.5 38.5 78-79 31.907115287625963 35.0 32.0 36.0 24.0 39.0 80-81 31.750097467989022 35.0 32.0 36.0 23.5 37.5 82-83 31.39073548100245 35.0 32.0 35.5 22.5 37.0 84-85 31.017085308961082 34.5 31.0 35.0 20.0 37.0 86-87 30.60445354053507 34.0 31.0 35.0 18.5 36.0 88-89 30.162835829066864 34.0 31.0 35.0 17.0 36.0 90-91 29.845479849426475 34.0 30.0 35.0 9.5 35.5 92-93 29.456309040715905 34.0 30.0 35.0 4.5 35.0 94-95 29.066028755960065 34.0 29.5 35.0 2.0 35.0 96-97 28.520533187228125 34.0 29.0 35.0 2.0 35.0 98-99 27.873507702874434 34.0 29.0 35.0 2.0 35.0 100-101 25.471062376194915 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03566089838961517 1101 2 0.06598502431711495 1101 3 0.04814651296160832 1101 4 -0.06426062722500347 1101 5 0.03118252511043096 1101 6 0.042312229813482816 1101 7 0.02294082906390571 1101 8 -0.006704751175355739 1101 9 -5.471326145851663E-4 1101 10-11 0.020051378093008054 1101 12-13 0.08513074657490449 1101 14-15 0.15629375483141672 1101 16-17 0.12537056497010468 1101 18-19 0.14072193400253497 1101 20-21 0.15031778106138205 1101 22-23 0.16069580857660526 1101 24-25 0.23681595470322492 1101 26-27 0.09004150887219708 1101 28-29 0.061344971491841704 1101 30-31 0.06148090235407011 1101 32-33 0.2259143525542484 1101 34-35 0.12254046231587523 1101 36-37 0.20952783098687178 1101 38-39 0.27635009527387666 1101 40-41 0.2849257540634085 1101 42-43 0.32811854989945033 1101 44-45 0.3392949154512266 1101 46-47 0.3426907847574654 1101 48-49 0.27408933722657025 1101 50-51 0.27471388306579314 1101 52-53 0.30984197949688763 1101 54-55 0.3830241540963044 1101 56-57 0.23384677657163166 1101 58-59 0.22295307727370783 1101 60-61 0.25796107785617295 1101 62-63 0.30988282676731416 1101 64-65 0.37503532420469554 1101 66-67 0.29460294326793957 1101 68-69 0.16528339749655885 1101 70-71 0.21957639136107332 1101 72-73 0.17882925746162215 1101 74-75 0.24514064773608624 1101 76-77 0.07467721929183568 1101 78-79 0.24082828526494993 1101 80-81 0.27035200950642135 1101 82-83 0.3056153658156049 1101 84-85 0.41180610306029664 1101 86-87 0.3852218702620469 1101 88-89 0.3747977300611858 1101 90-91 0.3500376337238933 1101 92-93 0.4855001530924099 1101 94-95 0.20561312806836085 1101 96-97 0.3377251509064578 1101 98-99 0.3973022850866208 1101 100-101 0.6604701687052845 1102 1 0.11287154268886468 1102 2 0.10684758290119589 1102 3 0.10758276606874517 1102 4 0.07638326531762374 1102 5 0.09681032871291961 1102 6 0.07006128541064527 1102 7 0.0813476292428632 1102 8 0.08615008471745256 1102 9 0.07289475221970321 1102 10-11 0.15473697986722357 1102 12-13 0.13082441315464166 1102 14-15 0.16980655062268823 1102 16-17 0.1966499527051937 1102 18-19 0.18461722789783153 1102 20-21 0.12884652224834525 1102 22-23 0.25815028704373333 1102 24-25 0.13255136879307372 1102 26-27 0.06845649108464613 1102 28-29 0.030031228785688313 1102 30-31 0.11598401128755853 1102 32-33 0.1311327913169933 1102 34-35 0.15264137406235534 1102 36-37 0.1989511172455991 1102 38-39 0.2677397012204423 1102 40-41 0.3154044269471399 1102 42-43 0.21637553752352545 1102 44-45 0.2822214039473323 1102 46-47 0.19817872822009264 1102 48-49 0.3548079685781076 1102 50-51 0.4211393870862352 1102 52-53 0.43450590605954886 1102 54-55 0.4377252347467504 1102 56-57 0.49895447079325095 1102 58-59 0.45553180542204785 1102 60-61 0.42940709677053945 1102 62-63 0.368246085130572 1102 64-65 0.4227849061314899 1102 66-67 0.3133142012197041 1102 68-69 0.4455168486160872 1102 70-71 0.557982665210389 1102 72-73 0.4978390878638521 1102 74-75 0.4283827904719004 1102 76-77 0.4218849209299158 1102 78-79 0.49250440592841116 1102 80-81 0.5609207804549392 1102 82-83 0.5454208391653808 1102 84-85 0.5531940343216455 1102 86-87 0.5746361849928192 1102 88-89 0.4926812150332758 1102 90-91 0.5766957515212496 1102 92-93 0.504077914708148 1102 94-95 0.29814343388980546 1102 96-97 0.5258413453517683 1102 98-99 0.37837690666756885 1102 100-101 0.599521794465776 1103 1 0.09920662086805265 1103 2 0.13397387374448044 1103 3 0.12153392695226728 1103 4 0.04478581639220636 1103 5 0.08183440035627854 1103 6 0.09989159783368962 1103 7 0.14740311037073894 1103 8 0.1102291833919864 1103 9 0.11934432602397038 1103 10-11 0.054654067684104746 1103 12-13 0.10983644784673174 1103 14-15 0.19020836972050148 1103 16-17 0.2096041341224648 1103 18-19 0.17613070282197896 1103 20-21 0.09260045605866907 1103 22-23 0.24212406239465167 1103 24-25 0.1909828826520581 1103 26-27 0.142580886469581 1103 28-29 0.19930537441744178 1103 30-31 0.09161709211903002 1103 32-33 0.1378020259268382 1103 34-35 0.08212704742450683 1103 36-37 0.09014943550639032 1103 38-39 0.08610000548782182 1103 40-41 0.14339158120672835 1103 42-43 0.1064695649093963 1103 44-45 0.13053977769376246 1103 46-47 0.17134308185524105 1103 48-49 0.2874249888452809 1103 50-51 0.22192253445077625 1103 52-53 0.13522150371632335 1103 54-55 0.2790120002001615 1103 56-57 0.32186105112882757 1103 58-59 0.3864041681029846 1103 60-61 0.35829114848786503 1103 62-63 0.3144713216110233 1103 64-65 0.18410400055874732 1103 66-67 0.2756372785946155 1103 68-69 0.2277682193781274 1103 70-71 0.2835006404361309 1103 72-73 0.19249497971203056 1103 74-75 0.06903912963920789 1103 76-77 0.1411017961572476 1103 78-79 0.05244460523394068 1103 80-81 0.17991094621152826 1103 82-83 0.12346255280431961 1103 84-85 0.10674559011114937 1103 86-87 0.14046281042178066 1103 88-89 0.014336657303129385 1103 90-91 -0.07992468463749702 1103 92-93 0.23840152525132652 1103 94-95 0.016270590001923324 1103 96-97 -0.0957128853158018 1103 98-99 2.7634119292585524E-4 1103 100-101 0.5069702228619555 1104 1 0.15161394234265657 1104 2 0.10784033417242966 1104 3 0.11106215587724932 1104 4 0.023476200511822753 1104 5 0.0014724514003603417 1104 6 0.03523060273185763 1104 7 0.06874263086571375 1104 8 0.002456045985439914 1104 9 0.05305920099174699 1104 10-11 0.12751356755520504 1104 12-13 0.1502484096925656 1104 14-15 0.14089926943692888 1104 16-17 0.08206507766461613 1104 18-19 0.15854885182945821 1104 20-21 0.1323663751099744 1104 22-23 0.13217820005898773 1104 24-25 0.08856370245097622 1104 26-27 0.16072783155851766 1104 28-29 0.10343942958629526 1104 30-31 0.15889886577203782 1104 32-33 0.08667493131482473 1104 34-35 0.1636717754471846 1104 36-37 0.23202414548318728 1104 38-39 0.1279422328660118 1104 40-41 0.150930122067777 1104 42-43 0.1633388611197617 1104 44-45 0.1461783443346505 1104 46-47 0.207404953471638 1104 48-49 0.06507541421265017 1104 50-51 0.15228035863226808 1104 52-53 0.15917149082640236 1104 54-55 0.2021947792669252 1104 56-57 0.25576979049464654 1104 58-59 0.37620669452731903 1104 60-61 0.15527748517258289 1104 62-63 0.0864512133356996 1104 64-65 0.23967141484077814 1104 66-67 0.1849556596206483 1104 68-69 0.1973511015642586 1104 70-71 0.11432714211182571 1104 72-73 0.10957618620854959 1104 74-75 0.23109532969076696 1104 76-77 0.21977413438875004 1104 78-79 0.1802713647080303 1104 80-81 0.130014581168993 1104 82-83 0.22862109882133552 1104 84-85 0.21920550245969395 1104 86-87 0.29025708729726674 1104 88-89 0.23096207622553067 1104 90-91 0.22235322203948016 1104 92-93 0.3522984058906644 1104 94-95 0.1759478264030605 1104 96-97 0.2187893059706134 1104 98-99 0.25176693155126273 1104 100-101 0.5025891968243137 1105 1 0.10686273357249121 1105 2 0.04923781610071387 1105 3 0.05346341970031432 1105 4 0.04303003267229144 1105 5 0.029338026583474175 1105 6 0.030090098160073353 1105 7 0.016154378675565795 1105 8 0.05604080351332641 1105 9 0.04890964859574609 1105 10-11 0.03543151480016604 1105 12-13 0.09626411173670135 1105 14-15 0.1034810110234119 1105 16-17 0.05613200284054898 1105 18-19 0.07267111713655794 1105 20-21 0.1329440954412462 1105 22-23 0.1968428560980584 1105 24-25 0.12754804827892485 1105 26-27 0.1561430261585457 1105 28-29 0.06124133489530692 1105 30-31 0.12026963507644695 1105 32-33 0.14275238834962778 1105 34-35 0.28363481819695835 1105 36-37 0.21540717004156562 1105 38-39 0.18162857495993734 1105 40-41 0.20609207333399127 1105 42-43 0.24336011632585297 1105 44-45 0.28277454329104046 1105 46-47 0.3442499915299422 1105 48-49 0.3834608802474051 1105 50-51 0.3639286902900736 1105 52-53 0.3722940912381034 1105 54-55 0.4565025010343646 1105 56-57 0.38035631928971014 1105 58-59 0.5013489224941949 1105 60-61 0.4526919138192369 1105 62-63 0.4430977737263362 1105 64-65 0.41984825432567874 1105 66-67 0.38522441351025805 1105 68-69 0.38799727897821157 1105 70-71 0.3236997305555036 1105 72-73 0.33222073597158186 1105 74-75 0.23410859025853625 1105 76-77 0.278107707082512 1105 78-79 0.5115969427634717 1105 80-81 0.6054929669222595 1105 82-83 0.6809591714911605 1105 84-85 0.7227426751343096 1105 86-87 0.7606227342885035 1105 88-89 0.4934499885238708 1105 90-91 0.4278500864178625 1105 92-93 0.47058812775384595 1105 94-95 0.5668996192126734 1105 96-97 0.734734827536986 1105 98-99 0.7930799671202244 1105 100-101 0.8670563326374214 1106 1 -0.15407871713748733 1106 2 -0.07916923230290962 1106 3 -0.05226697754008569 1106 4 -0.0012538382207338827 1106 5 -0.01993200051060029 1106 6 -0.07290230127672714 1106 7 0.05375005071731209 1106 8 -0.10828864578738973 1106 9 -0.12886622716635543 1106 10-11 0.023928612587631903 1106 12-13 -0.1332812930467142 1106 14-15 -0.2952120750028513 1106 16-17 -0.1797285664624937 1106 18-19 -0.08084169369296745 1106 20-21 -0.012225159725993251 1106 22-23 0.03129061781064024 1106 24-25 0.00949720378094554 1106 26-27 -0.023027453335785708 1106 28-29 0.02392867313476188 1106 30-31 0.06921731273642706 1106 32-33 0.15201265122708918 1106 34-35 0.2085776506630168 1106 36-37 0.04384774901111399 1106 38-39 0.004576420182267782 1106 40-41 0.08890473590883374 1106 42-43 -0.05934516519970856 1106 44-45 0.10594025576308752 1106 46-47 0.15106616024749542 1106 48-49 0.19217859214908373 1106 50-51 0.13125940571799077 1106 52-53 0.04684231074333667 1106 54-55 -0.32057931191791766 1106 56-57 -0.08287559659761001 1106 58-59 0.03522746077866401 1106 60-61 0.015657810899398328 1106 62-63 0.0042910565388325494 1106 64-65 -0.15550549495436172 1106 66-67 -0.13771670026547866 1106 68-69 -0.18184120792287928 1106 70-71 -0.27116336141143904 1106 72-73 -0.1610504412589009 1106 74-75 -0.22959886975857557 1106 76-77 -0.14676980139919849 1106 78-79 -0.08877245441558657 1106 80-81 -0.28711883616265865 1106 82-83 -0.4188057526416351 1106 84-85 -0.34607668522988533 1106 86-87 -0.19863889478309105 1106 88-89 -0.11659450597615617 1106 90-91 -0.0844149892629602 1106 92-93 -0.04919517757100067 1106 94-95 -0.13215278491804838 1106 96-97 0.08633076847469567 1106 98-99 0.15373006562191804 1106 100-101 -0.1760101778548595 1107 1 0.07094968241486299 1107 2 0.08149617051418545 1107 3 0.03739187910228026 1107 4 0.08445494098514672 1107 5 0.029835066723265413 1107 6 0.013947008668480976 1107 7 0.008376806557649275 1107 8 8.300146077289128E-5 1107 9 0.0032557513327020615 1107 10-11 0.04011926350386119 1107 12-13 0.10701295040656333 1107 14-15 0.16063051692990626 1107 16-17 0.0750887439301593 1107 18-19 0.1387734807905474 1107 20-21 0.06205590579589426 1107 22-23 0.07596480254595406 1107 24-25 0.11797726041326939 1107 26-27 0.047665657471071654 1107 28-29 0.1505665856232028 1107 30-31 0.07058679196537554 1107 32-33 0.07651608654948205 1107 34-35 0.07454060817883601 1107 36-37 0.04351873032072717 1107 38-39 -0.013093467093874267 1107 40-41 0.011534213523852088 1107 42-43 0.003277890401754746 1107 44-45 0.014588292188641105 1107 46-47 0.026286622143913974 1107 48-49 -0.018359002153175652 1107 50-51 -0.08164904369424875 1107 52-53 -0.11693520793011913 1107 54-55 -0.01691552959789533 1107 56-57 0.009382305536767888 1107 58-59 0.021394019035945178 1107 60-61 0.11758394325521238 1107 62-63 0.2449470015426769 1107 64-65 -0.012226699880436342 1107 66-67 -0.09285501025500054 1107 68-69 0.1433182094663934 1107 70-71 0.009516873359316946 1107 72-73 -0.0985208855924995 1107 74-75 -0.012174626185689874 1107 76-77 0.02607577899364344 1107 78-79 -0.017995783306407276 1107 80-81 0.07811566420148708 1107 82-83 0.011184275716644265 1107 84-85 0.1253651494713246 1107 86-87 0.060219381721655196 1107 88-89 0.2434249723346369 1107 90-91 0.17890677197931382 1107 92-93 0.25859523701782194 1107 94-95 0.21674365472235735 1107 96-97 0.21300591246880884 1107 98-99 0.3734099319523878 1107 100-101 0.48983267857321167 1108 1 0.056478356637242655 1108 2 0.0630621130143183 1108 3 0.008886045209521853 1108 4 -0.017201284634936087 1108 5 -0.012280142374578418 1108 6 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-0.38024583824538993 2316 100-101 -0.5003725631867901 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 9.0 5 32.0 6 65.0 7 154.0 8 423.0 9 817.0 10 1471.0 11 2395.0 12 3579.0 13 5419.0 14 7424.0 15 10017.0 16 12686.0 17 18341.0 18 18675.0 19 12911.0 20 11102.0 21 11108.0 22 11498.0 23 12207.0 24 14430.0 25 16703.0 26 19715.0 27 24081.0 28 29439.0 29 36699.0 30 44718.0 31 55608.0 32 70393.0 33 92283.0 34 129461.0 35 196947.0 36 332829.0 37 580554.0 38 554784.0 39 72054.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57866288850325 18.89427336162532 15.101814646576925 23.4252491032945 2 15.611090415977714 19.8430055196548 40.03807259262824 24.50783147173925 3 19.176480445444348 23.47413575699657 29.909378090137444 27.440005707421637 4 13.142666591457324 16.84562065956381 36.33449022997468 33.67722251900418 5 13.953724687355873 36.868034149465295 34.410886884043784 14.767354279135049 6 32.90162618081432 35.756359889973155 16.980333863116787 14.361680066095738 7 28.942476466665116 32.43012996837889 20.193169111523286 18.434224453432698 8 26.34978872222987 35.21387415575991 19.342243443089384 19.09409367892083 9 26.043637636569812 16.893553730799763 20.052442143176506 37.01036648945392 10-11 25.045809954841214 26.45028220093503 26.93071643534264 21.57319140888112 12-13 25.70682956125303 23.71074735965439 27.547294786333577 23.035128292758998 14-15 22.732251701334853 25.19582770645794 25.67429184321507 26.39762874899214 16-17 22.242056904477547 28.44535377983074 26.589391592551014 22.723197723140697 18-19 22.219881147119427 26.907801088987032 28.869976873127367 22.002340890766174 20-21 22.692538680275135 26.017068096528977 29.294812878051367 21.995580345144518 22-23 22.614025104435914 25.861471857880886 29.063378248794713 22.461124788888483 24-25 22.502889979207083 26.20310031411285 29.034429638183312 22.25958006849676 26-27 22.102241319050123 26.4532067217792 29.001679096544418 22.442872862626256 28-29 22.25532678223884 26.72330180139545 28.90254132280679 22.11883009355892 30-31 22.29476559570776 26.33856729521768 28.883580915850697 22.48308619322386 32-33 22.051415199594533 26.279324506706242 28.94359193011475 22.725668363584482 34-35 22.61270426984517 26.41465132173233 28.77637734682808 22.196267061594416 36-37 22.663382779668215 26.056423903467657 28.755613936997815 22.52457937986631 38-39 22.234959932216118 26.459795839421886 28.71022908173372 22.59501514662828 40-41 22.721319098601576 26.272936248393346 28.636173289945788 22.36957136305929 42-43 22.324985248871503 26.247426978025224 28.580376280807563 22.84721149229571 44-45 22.400140026279814 26.535457009941204 28.640123335943617 22.424279627835368 46-47 22.67791185279919 26.687208230948677 28.061341828123183 22.573538088128952 48-49 22.529103526765123 26.595758359020643 28.1462252099502 22.728912904264035 50-51 22.693907379695467 26.88613001483173 27.92271244703548 22.497250158437325 52-53 22.81679999734555 26.692936017864433 27.86336066308095 22.626903321709065 54-55 22.58107262899785 26.79363911460908 27.869188004552374 22.756100251840692 56-57 22.6204953198775 26.983888334035655 27.798782935885143 22.596833410201704 58-59 22.604423470623562 26.714441189059695 27.83376772258371 22.847367617733035 60-61 22.496545070857156 26.97503326354349 27.824249040251374 22.704172625347983 62-63 22.3970655084428 26.948198459756917 28.007903617016332 22.646832414783944 64-65 22.275168308552963 26.8835792568211 27.888059466256998 22.953192968368942 66-67 22.305984783380506 26.89971331968505 27.73903713240052 23.055264764533927 68-69 22.4517952896368 26.67133407478208 28.036447259083246 22.840423376497874 70-71 22.44424416270435 26.73540717563483 27.699925509570946 23.12042315208988 72-73 22.49301963295629 26.78795693822769 27.545490591643134 23.173532837172882 74-75 22.359426274383587 27.137410785683237 27.429005975824623 23.074156964108553 76-77 22.475682773632048 26.80813488574263 27.605277870867774 23.11090446975755 78-79 22.4779432014626 26.8884733941423 27.631988247434307 23.001595156960793 80-81 22.557618098022107 27.033638484177835 27.392548800355698 23.016194617444363 82-83 22.57114387003989 27.050690623059065 27.360721843928832 23.017443662972216 84-85 22.51592288000478 26.99166651458567 27.412405495488173 23.080005109921377 86-87 22.53238798767586 26.965785103983116 27.35551310730782 23.146313801033205 88-89 22.468019080507347 27.053143302473988 27.165563342398553 23.313274274620113 90-91 22.46966925316777 26.99996910051602 27.24119245463634 23.289169191679868 92-93 22.36133905533829 26.80561897900618 27.404550644679503 23.428491320976025 94-95 22.25119533082709 26.799528669690524 27.418491875732048 23.530784123750337 96-97 22.42597409922988 26.584995404488005 27.412913388700684 23.576117107581435 98-99 22.204804715625738 26.738123836605492 27.22942471489576 23.82764673287301 100-101 22.31083744496169 26.44105384878277 27.45623479914129 23.79187390711425 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 594.0 1 395.0 2 271.0 3 380.5 4 329.5 5 291.0 6 641.0 7 1515.5 8 2507.0 9 2966.0 10 2648.0 11 2205.0 12 2208.0 13 2392.0 14 2566.0 15 2605.0 16 2642.5 17 2867.5 18 3149.5 19 3415.0 20 3741.5 21 4480.0 22 5501.5 23 6719.5 24 8453.0 25 10841.0 26 13868.0 27 17163.5 28 19650.0 29 22135.5 30 25726.0 31 30222.0 32 36207.0 33 39834.5 34 42124.5 35 46111.0 36 51049.0 37 56652.5 38 60725.0 39 63706.0 40 66938.0 41 71896.0 42 75350.5 43 77176.0 44 81123.5 45 84997.0 46 87693.5 47 91119.0 48 91085.0 49 89781.0 50 90643.5 51 88903.5 52 87837.0 53 89443.0 54 91182.5 55 85993.0 56 76934.5 57 69951.0 58 64321.0 59 56679.0 60 44767.5 61 35743.5 62 29093.5 63 21664.0 64 15889.5 65 12009.0 66 8778.0 67 6618.0 68 4950.0 69 3486.0 70 2477.0 71 1656.0 72 1148.0 73 866.5 74 629.0 75 445.5 76 264.0 77 136.5 78 83.5 79 46.5 80 21.5 81 11.0 82 5.5 83 4.0 84 2.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01617553860395977 3 0.006304312481543296 4 0.0 5 0.0 6 0.0 7 0.0 8 1.6590296004061303E-4 9 4.147574001015326E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.1106805007614946E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005433321941330077 26-27 0.02179550137533554 28-29 2.073787000507663E-5 30-31 0.0 32-33 4.147574001015326E-4 34-35 0.019929093074878643 36-37 0.0034839621608528737 38-39 0.018477442174523278 40-41 0.015408237413771937 42-43 0.008938021972188027 44-45 0.002986253280731035 46-47 6.22136100152299E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.0368935002538315E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.073787000507663E-5 84-85 0.00215673848052797 86-87 3.1106805007614946E-4 88-89 4.147574001015326E-5 90-91 1.866408300456897E-4 92-93 2.073787000507663E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2411048.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.82012802203633 #Duplication Level Percentage of deduplicated Percentage of total 1 72.48427680842721 36.836602269889895 2 14.9692459012814 15.214779861929264 3 5.2932054512799755 8.070041360429666 4 2.3950623471351675 4.868695004086721 5 1.3474226368981674 3.4238095453477313 6 0.8000636033718691 2.4395600849478045 7 0.5154299088411867 1.8335949767586932 8 0.35448593528353095 1.4412016490496262 9 0.2556977935353139 1.1695135142175177 >10 1.4156249309282827 13.255924416817832 >50 0.11286915386227278 3.9292657014928234 >100 0.052447768300212215 4.882267822319573 >500 0.002860228038038192 0.9757618166454769 >1k 0.001144091215214572 0.9563254884820089 >5k 1.6344160217351028E-4 0.7026564875853176 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9077 0.3764752920721611 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7842 0.3252527531596219 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4681 0.1941479389875274 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.244272200304598E-4 2 0.0 0.0 0.0 0.0 1.244272200304598E-4 3 0.0 0.0 0.0 0.0 1.244272200304598E-4 4 0.0 0.0 0.0 0.0 1.244272200304598E-4 5 0.0 0.0 0.0 0.0 1.6590296004061303E-4 6 0.0 0.0 0.0 0.0 1.6590296004061303E-4 7 0.0 0.0 0.0 0.0 1.6590296004061303E-4 8 0.0 0.0 0.0 0.0 1.6590296004061303E-4 9 0.0 0.0 0.0 0.0 2.073787000507663E-4 10-11 0.0 0.0 0.0 4.147574001015326E-5 2.073787000507663E-4 12-13 0.0 0.0 0.0 4.147574001015326E-5 4.354952701066092E-4 14-15 0.0 0.0 0.0 4.147574001015326E-5 5.391846201319924E-4 16-17 0.0 0.0 0.0 6.221361001522988E-5 6.636118401624521E-4 18-19 0.0 0.0 0.0 1.0368935002538315E-4 7.050875801726055E-4 20-21 0.0 0.0 0.0 1.4516509003553642E-4 7.88039060192912E-4 22-23 0.0 0.0 0.0 2.488544400609196E-4 9.332041502284484E-4 24-25 0.0 0.0 0.0 4.354952701066092E-4 9.954177602436783E-4 26-27 0.0 0.0 0.0 0.0010368935002538315 9.954177602436783E-4 28-29 0.0 0.0 0.0 0.004085360391000097 9.954177602436783E-4 30-31 0.0 0.0 0.0 0.013749207813365806 9.954177602436783E-4 32-33 0.0 0.0 0.0 0.033429446448183534 0.0010783692402639847 34-35 0.0 0.0 0.0 0.06210992066520451 0.0010783692402639847 36-37 0.0 0.0 0.0 0.10814799207647463 0.0010783692402639847 38-39 0.0 0.0 0.0 0.17380408851254725 0.0010783692402639847 40-41 0.0 0.0 0.0 0.24373218616966563 0.0010783692402639847 42-43 0.0 0.0 0.0 0.31585849804732213 0.0010783692402639847 44-45 0.0 0.0 0.0 0.40459584379904506 0.001119844980274138 46-47 0.0 0.0 0.0 0.5071860867141591 0.0012027964602944446 48-49 0.0 0.0 0.0 0.612679631429984 0.0012442722003045978 50-51 0.0 0.0 0.0 0.7199566329662455 0.0013272236803249043 52-53 0.0 0.0 0.0 0.8249732066719535 0.0015346023803756706 54-55 0.0 0.0 0.0 0.9412504437904181 0.001679767470411207 56-57 0.0 0.0 0.0 1.0749474917131472 0.0017212432104213604 58-59 0.0 0.0 0.0 1.2147207355473637 0.0017834568204365903 60-61 0.0 0.0 0.0 1.3607775539931184 0.0018664083004568967 62-63 0.0 0.0 0.0 1.5063366635587512 0.00190788404046705 64-65 0.0 0.0 0.0 1.6603153483464452 0.00190788404046705 66-67 0.0 0.0 0.0 1.8383707002100331 0.00190788404046705 68-69 0.0 0.0 0.0 2.027997783536454 0.00190788404046705 70-71 0.0 0.0 0.0 2.226106655694951 0.0019700976504822797 72-73 0.0 0.0 0.0 2.4289852379546155 0.002073787000507663 74-75 0.0 0.0 0.0 2.629665605993742 0.0021152627405178165 76-77 0.0 0.0 0.0 2.8496736688776 0.0021152627405178165 78-79 0.0 0.0 0.0 3.089486397616306 0.0021152627405178165 80-81 0.0 0.0 0.0 3.329195437004987 0.002136000610522893 82-83 0.0 0.0 0.0 3.583752791317303 0.0022811657005584293 84-85 0.0 0.0 0.0 3.8470822646417657 0.0023226414405685828 86-87 0.0 0.0 0.0 4.1165501474877315 0.0023848550505838123 88-89 0.0 0.0 0.0 4.412189222280103 0.002447068660599042 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4365 0.0 55.509586 1 GTATCAA 7500 0.0 44.08898 1 ATCAACG 9485 0.0 34.704666 3 CCGATCG 110 7.023482E-8 34.54246 9 TATCAAC 9605 0.0 34.37712 2 TCAACGC 9620 0.0 34.313553 4 CAACGCA 9760 0.0 33.870018 5 AACGCAG 10105 0.0 32.713642 6 CGCAGAG 11920 0.0 27.453577 8 ACGCAGA 12035 0.0 27.270176 7 TGGTATC 1710 0.0 26.394264 2 GCAGAGT 13195 0.0 24.548838 9 GTGGTAT 1950 0.0 24.120298 1 GAGTACT 8360 0.0 23.208214 12-13 GTGTAAG 950 0.0 23.004684 1 CAGAGTA 12605 0.0 21.779152 10-11 GTACTTT 8920 0.0 21.564835 14-15 GTACATG 7350 0.0 21.007679 1 TACATGG 7080 0.0 20.936468 2 AGAGTAC 12115 0.0 20.503794 10-11 >>END_MODULE