FastQCFastQC Report
Thu 26 May 2016
SRR938132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356181
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86470.3669921792935263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71740.30447575971455504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43640.18521497287347619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42050.053.1052861
GTATCAA75100.043.112171
ATCAACG92750.034.254113
TATCAAC93800.033.9206542
TCAACGC95000.033.4428254
TGGTATC18750.033.228512
CAACGCA96400.032.9078065
AACGCAG99550.031.908876
GTGGTAT22600.029.0737861
CGCAGAG114450.027.4124168
ACGCAGA115800.027.2194637
GCAGAGT123400.024.6152869
TACATGG73500.022.3240342
GTACATG75400.022.2912431
GAGTACT78750.022.27312312-13
CAGAGTA117500.021.52205510-11
ACCGAGT8750.021.1873058
ACATGGG75250.020.9204793
AGAGTAC113850.020.64634510-11
GGACCGA9000.020.6062186
Produced by FastQC (version 0.11.5)