FastQCFastQC Report
Thu 26 May 2016
SRR938132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356181
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83900.3560846980771002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74210.314958825319447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43710.18551206380155005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43650.053.980181
GTATCAA73800.041.132151
ATCAACG92250.032.6420063
TATCAAC93100.032.244692
TCAACGC93450.032.0676464
CAACGCA95350.031.4784565
AACGCAG99200.030.2567636
GGACCGA7100.028.7625356
TGGTATC19800.027.8281042
ACGCAGA113800.026.0822817
CGCAGAG113700.026.0216858
AGGACCG8950.026.0010035
GTGGTAT22900.024.6858651
GCAGAGT123850.023.620699
ACCGAGT9000.022.6899648
GAGTACT79000.022.66372712-13
CAGAGTA118500.021.3812510-11
GTACTTT82650.021.28889814-15
GTACATG73150.020.8462331
TACATGG71100.020.4429072
Produced by FastQC (version 0.11.5)