##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938132_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2356181 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56387561057491 34.0 31.0 34.0 31.0 34.0 2 32.77651844234378 34.0 31.0 34.0 31.0 34.0 3 32.832325699935616 34.0 31.0 34.0 31.0 34.0 4 36.27249477013863 37.0 37.0 37.0 35.0 37.0 5 36.246896991360174 37.0 37.0 37.0 35.0 37.0 6 36.272330945712575 37.0 37.0 37.0 35.0 37.0 7 36.23412377911544 37.0 37.0 37.0 35.0 37.0 8 36.21709792244314 37.0 37.0 37.0 35.0 37.0 9 37.92216472333832 39.0 38.0 39.0 35.0 39.0 10-11 37.9003584614255 39.0 38.0 39.0 35.0 39.0 12-13 37.82238524969007 39.0 38.0 39.0 35.0 39.0 14-15 39.13145361073703 40.0 38.5 41.0 36.0 41.0 16-17 39.01541328955628 40.0 38.0 41.0 36.0 41.0 18-19 39.25461371600909 40.5 39.0 41.0 36.0 41.0 20-21 39.232473651217795 41.0 39.0 41.0 36.0 41.0 22-23 39.147989055170214 40.5 39.0 41.0 36.0 41.0 24-25 39.056198356577866 40.0 39.0 41.0 35.0 41.0 26-27 38.89660004897756 40.0 38.0 41.0 35.0 41.0 28-29 38.78652870895742 40.0 38.0 41.0 35.0 41.0 30-31 38.57000077668057 40.0 38.0 41.0 34.0 41.0 32-33 38.37341613398971 40.0 38.0 41.0 34.0 41.0 34-35 38.23944722413091 40.0 38.0 41.0 34.0 41.0 36-37 38.25669505016805 40.0 38.0 41.0 34.0 41.0 38-39 38.123355548661166 40.0 38.0 41.0 34.0 41.0 40-41 37.9657532676819 40.0 38.0 41.0 33.0 41.0 42-43 37.75051471003289 40.0 37.5 41.0 33.0 41.0 44-45 37.50020987351991 40.0 37.0 41.0 33.0 41.0 46-47 37.13808956103118 40.0 37.0 41.0 31.5 41.0 48-49 36.77364069228977 40.0 36.0 41.0 31.0 41.0 50-51 36.419275301854995 39.0 36.0 41.0 30.0 41.0 52-53 35.93001959526879 39.0 35.0 40.0 29.0 41.0 54-55 35.63551548034722 39.0 35.0 40.0 28.0 41.0 56-57 35.30822610826588 38.0 35.0 40.0 28.0 41.0 58-59 34.89342287370962 38.0 34.0 40.0 26.5 41.0 60-61 34.43866472906793 37.5 34.0 40.0 26.0 41.0 62-63 33.988853785002085 37.0 33.0 40.0 25.5 41.0 64-65 33.513029983689705 36.0 33.0 39.0 23.5 40.5 66-67 32.97595494573634 36.0 32.0 39.0 22.0 40.0 68-69 32.41018516828716 35.0 31.5 38.5 20.5 40.0 70-71 31.814271696444372 35.0 31.0 38.0 18.5 40.0 72-73 31.246043703773182 35.0 30.5 37.0 16.5 39.0 74-75 30.649974895816577 34.0 30.0 36.5 11.5 39.0 76-77 29.27810002712016 32.5 28.5 35.0 9.0 37.0 78-79 29.968121931209872 34.0 29.5 35.5 7.5 37.5 80-81 29.661435390574834 34.0 29.0 35.0 4.5 37.0 82-83 29.12159952906844 34.0 29.0 35.0 2.0 36.5 84-85 28.553290473015444 34.0 28.5 35.0 2.0 36.0 86-87 27.936798998039627 33.0 26.5 35.0 2.0 36.0 88-89 27.231459510114036 33.0 26.0 35.0 2.0 35.0 90-91 26.724980381388356 32.5 25.0 35.0 2.0 35.0 92-93 25.94021872682956 32.0 23.5 34.0 2.0 35.0 94-95 25.155503970195838 32.0 19.5 34.0 2.0 35.0 96-97 24.159247952512985 31.0 9.5 34.0 2.0 35.0 98-99 22.76282912900155 31.0 2.0 34.0 2.0 35.0 100-101 18.347164967377296 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06259756696914565 1101 2 0.11441170486818919 1101 3 0.12353770408375198 1101 4 0.03958480068635595 1101 5 0.1012447742942939 1101 6 0.030492746910610435 1101 7 0.04039577831640173 1101 8 0.026277483874096674 1101 9 -0.0517233951816749 1101 10-11 0.141905983067538 1101 12-13 0.2309387247641439 1101 14-15 0.20401962133708906 1101 16-17 0.20320672127314054 1101 18-19 0.2273925648752524 1101 20-21 0.22519016478346998 1101 22-23 0.19961244973254821 1101 24-25 0.24328289251864277 1101 26-27 0.2649742919785396 1101 28-29 0.25416029415948316 1101 30-31 0.25640563554349427 1101 32-33 0.3080898724156498 1101 34-35 0.2752134048887527 1101 36-37 0.3465650946729468 1101 38-39 0.4081725653717214 1101 40-41 0.23962506487849566 1101 42-43 0.4328751783498248 1101 44-45 0.34543343288703454 1101 46-47 0.22697120752649624 1101 48-49 0.28645820009259637 1101 50-51 0.13922956937286557 1101 52-53 0.012833758421066932 1101 54-55 -0.07467944808851001 1101 56-57 0.226729963622887 1101 58-59 0.3350589481988635 1101 60-61 0.11518460493676486 1101 62-63 0.16992155711579926 1101 64-65 0.34651470798667106 1101 66-67 0.04804789053506653 1101 68-69 0.06915285973353491 1101 70-71 -0.01530948371394203 1101 72-73 -0.09067562428269582 1101 74-75 -0.20954810238886878 1101 76-77 -0.06498548463316567 1101 78-79 -0.03436580697503189 1101 80-81 -0.2340693738991213 1101 82-83 -0.14351808638644314 1101 84-85 -0.06593414766294359 1101 86-87 -0.23665511357241442 1101 88-89 -0.06949054544319822 1101 90-91 -0.30303212920145484 1101 92-93 -0.36063118249660775 1101 94-95 -0.418263494171061 1101 96-97 -0.2978650311990094 1101 98-99 -0.1901796964655631 1101 100-101 -0.37902232698584015 1102 1 0.16189799831175833 1102 2 0.07261554454444052 1102 3 0.0325180513387906 1102 4 0.06362291271426557 1102 5 0.008751468586275735 1102 6 0.05068164104991979 1102 7 0.019480659515437537 1102 8 0.06719217860226934 1102 9 0.13302240346856564 1102 10-11 0.12252282723896002 1102 12-13 0.06302520835252778 1102 14-15 0.16232039193794634 1102 16-17 0.17246863771868703 1102 18-19 0.13677994628633172 1102 20-21 0.11008128409331874 1102 22-23 0.09936656323865378 1102 24-25 0.08296633732253866 1102 26-27 0.09440274786891223 1102 28-29 0.03817358278342198 1102 30-31 0.11336095947452662 1102 32-33 0.10585675051289911 1102 34-35 0.1978572261481304 1102 36-37 0.2921887391545468 1102 38-39 0.15176868752892148 1102 40-41 0.1089352953317615 1102 42-43 0.21535006718216465 1102 44-45 0.09847539791920212 1102 46-47 0.13091493306658464 1102 48-49 0.3379058028696633 1102 50-51 0.38313049385155296 1102 52-53 0.28520624881884515 1102 54-55 0.45740393068119545 1102 56-57 0.3891335627892758 1102 58-59 0.45676973358168027 1102 60-61 0.24847598535429682 1102 62-63 0.35708608524394236 1102 64-65 0.26820020045635573 1102 66-67 0.2519220227003842 1102 68-69 0.3826320135265604 1102 70-71 0.4137881953962328 1102 72-73 0.39839768578721646 1102 74-75 0.31586218804041266 1102 76-77 0.4016274913885489 1102 78-79 0.41408057204911586 1102 80-81 0.34341970622640616 1102 82-83 0.569024169438503 1102 84-85 0.3501691080044864 1102 86-87 0.4783221530966735 1102 88-89 0.23303654977931743 1102 90-91 -0.1411828537601174 1102 92-93 -0.30116216707247645 1102 94-95 -0.38571931487308575 1102 96-97 -0.5189447811172201 1102 98-99 -0.6002289343461698 1102 100-101 -1.2979971612945427 1103 1 0.1171765449017812 1103 2 0.10123806956040937 1103 3 0.14652841256226168 1103 4 0.03965158349704012 1103 5 0.002441057685700798 1103 6 0.06329181775846138 1103 7 0.08685628935008793 1103 8 0.044305939158647334 1103 9 0.06939739459765093 1103 10-11 0.11578519328821102 1103 12-13 0.12603599537970922 1103 14-15 0.12176933097705245 1103 16-17 0.19088819630217557 1103 18-19 0.19351190285434683 1103 20-21 0.16844655270448783 1103 22-23 0.19662900025519292 1103 24-25 0.18321756150818658 1103 26-27 0.19593102833161424 1103 28-29 0.18052934177853075 1103 30-31 0.17079442764569563 1103 32-33 0.19517901294992157 1103 34-35 0.16402002509781255 1103 36-37 0.21516120384833215 1103 38-39 0.11138830288623325 1103 40-41 0.23129318334539306 1103 42-43 0.17598203084691733 1103 44-45 0.016748763897489027 1103 46-47 0.18775227605146938 1103 48-49 0.050976202415476735 1103 50-51 -0.07590399624850619 1103 52-53 -0.16629080603886592 1103 54-55 -0.12464170128247076 1103 56-57 -0.14063792255597463 1103 58-59 -0.09406271266060173 1103 60-61 -0.19542224756033022 1103 62-63 -0.18369632906306066 1103 64-65 -0.22643680188556914 1103 66-67 -0.14031593060314407 1103 68-69 -0.17740347011547897 1103 70-71 -0.3379590191378661 1103 72-73 -0.5308120935045295 1103 74-75 -0.33595634861418944 1103 76-77 -0.19437137313490993 1103 78-79 -0.21402318212020788 1103 80-81 -0.2795499197760094 1103 82-83 -0.2819849853411469 1103 84-85 -0.3487564473145106 1103 86-87 -0.4961164956862376 1103 88-89 -0.5827149937591152 1103 90-91 -0.4487681919192248 1103 92-93 -0.5460188131586285 1103 94-95 -0.6311046672256531 1103 96-97 -0.5901182251595181 1103 98-99 -0.5819378265468664 1103 100-101 -1.1310838833156893 1104 1 0.07903210631453561 1104 2 0.1260236959881027 1104 3 0.09607242943100402 1104 4 0.11433617320007983 1104 5 0.032540337249926665 1104 6 0.09146085659170922 1104 7 0.07949554431406369 1104 8 0.07667986289789042 1104 9 0.1280496525488033 1104 10-11 0.1352571665793718 1104 12-13 0.13218429971989565 1104 14-15 0.10228110395191692 1104 16-17 0.11531052981123224 1104 18-19 0.13813323478195372 1104 20-21 0.11922629264474693 1104 22-23 0.020117842286474286 1104 24-25 0.2024009211989224 1104 26-27 0.15271410687668663 1104 28-29 0.016114476201302352 1104 30-31 0.04981700715720905 1104 32-33 0.04546630868001955 1104 34-35 -0.045754179787088844 1104 36-37 -0.018383867316664748 1104 38-39 0.0010056812028764739 1104 40-41 0.02941390026684587 1104 42-43 -0.023721887620830273 1104 44-45 -0.14631835963088946 1104 46-47 -0.08465503373591332 1104 48-49 -0.0784935744251456 1104 50-51 -0.07876948561768415 1104 52-53 -0.05277033511986673 1104 54-55 -0.15002520154605037 1104 56-57 0.03455272935053699 1104 58-59 -0.034193181822438135 1104 60-61 -0.20612423315161976 1104 62-63 -0.07872092930906405 1104 64-65 -0.06901850984551317 1104 66-67 -0.24300658250965768 1104 68-69 -0.18003525714763668 1104 70-71 -0.2938332064511684 1104 72-73 -0.32967301425659556 1104 74-75 -0.4616218731265782 1104 76-77 -0.3986685089634072 1104 78-79 -0.20408687918575197 1104 80-81 -0.1161203819092016 1104 82-83 -0.2868707699132784 1104 84-85 -0.2829731930533974 1104 86-87 -0.42987110823236563 1104 88-89 -0.4865425284630298 1104 90-91 -0.43338249520511596 1104 92-93 -0.5678910675954576 1104 94-95 -0.7012966370024927 1104 96-97 -0.7426344788934856 1104 98-99 -0.7327004341729406 1104 100-101 -1.1306292180547537 1105 1 0.10347039340203423 1105 2 0.054378215298420685 1105 3 0.045618397310612124 1105 4 0.07343060400203427 1105 5 0.0017199934592966315 1105 6 -0.015481271548438258 1105 7 0.1037746082790818 1105 8 0.016304752919026555 1105 9 0.08815523225228361 1105 10-11 0.13688797247818485 1105 12-13 0.07887492098554105 1105 14-15 0.06376306085333994 1105 16-17 0.01980702361958464 1105 18-19 0.1179086152759723 1105 20-21 0.04426421072856357 1105 22-23 0.08795674131603448 1105 24-25 0.11152801736312057 1105 26-27 0.09231765771646394 1105 28-29 0.047569250268615804 1105 30-31 0.015196647608412661 1105 32-33 -0.015226426806052018 1105 34-35 0.14007069447588805 1105 36-37 0.011959627844255749 1105 38-39 0.14997286482488903 1105 40-41 -0.04126146840854972 1105 42-43 -0.08267794203902668 1105 44-45 -0.14458660298948445 1105 46-47 -0.210861518220689 1105 48-49 -0.1715496406619721 1105 50-51 -0.21471648565707824 1105 52-53 -0.3177808077334916 1105 54-55 -0.19571567179594496 1105 56-57 -0.1867025726152889 1105 58-59 -0.22154873197936809 1105 60-61 -0.3083192141761586 1105 62-63 -0.3465284761567773 1105 64-65 -0.3972692879048836 1105 66-67 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0.38374811702097134 1106 24-25 0.3966518490663802 1106 26-27 0.35098772864969874 1106 28-29 0.2703046149081061 1106 30-31 0.29235276443636593 1106 32-33 0.3393167532348045 1106 34-35 0.3253923571865087 1106 36-37 0.27683248029695307 1106 38-39 0.13868973652842698 1106 40-41 0.19745346546405074 1106 42-43 0.19472793966543378 1106 44-45 0.2228304492337756 1106 46-47 0.18317314914417437 1106 48-49 0.15714524311984945 1106 50-51 0.17535747058411033 1106 52-53 0.07968877262706542 1106 54-55 -0.024924511405551186 1106 56-57 -0.011841536625034621 1106 58-59 0.16143168645432127 1106 60-61 8.996074159242085E-4 1106 62-63 0.26376575548034253 1106 64-65 0.07153443307502272 1106 66-67 0.11322553438556326 1106 68-69 -0.07038369415087686 1106 70-71 -0.6118372286985547 1106 72-73 -0.31543685725320714 1106 74-75 -0.1340098681827122 1106 76-77 -0.04289117214841198 1106 78-79 -0.12690844464729167 1106 80-81 -0.06883240721435158 1106 82-83 -0.22569447688219668 1106 84-85 -0.276180962134962 1106 86-87 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2316 96-97 2.1992890502266462 2316 98-99 2.6828926277077763 2316 100-101 3.1416539834240957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 17.0 5 29.0 6 64.0 7 178.0 8 486.0 9 887.0 10 1553.0 11 2773.0 12 4250.0 13 6299.0 14 8656.0 15 11636.0 16 15394.0 17 21457.0 18 22397.0 19 17598.0 20 16474.0 21 17143.0 22 18672.0 23 20650.0 24 23377.0 25 26555.0 26 30300.0 27 36192.0 28 44147.0 29 52616.0 30 63646.0 31 78280.0 32 97174.0 33 126968.0 34 171568.0 35 246659.0 36 366338.0 37 488746.0 38 298969.0 39 18023.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50979203699837 18.852493807289996 15.136528245379024 23.501185910332616 2 15.624051565143017 19.783051164014765 40.025553355363556 24.56734391547867 3 19.188158677189232 23.396476924624558 29.890791539559576 27.52457285862664 4 13.124809278072677 16.80823061405776 36.315534600724305 33.75142550714526 5 13.958795597575033 36.875020159955454 34.31546977364827 14.85071446882125 6 32.83404084827094 35.74155799288156 17.008710030923538 14.415691127923964 7 28.899095612773383 32.46168269755167 20.152823573401193 18.48639811627375 8 26.355615294410743 35.13274234874146 19.40576721397889 19.105875142868907 9 26.065951639538724 16.772438110654488 20.14132191032862 37.020288339478164 10-11 25.088628590078603 26.425325558605216 26.945446890540243 21.540598960775935 12-13 25.74663986595115 23.65528852928244 27.560420447542523 23.037651157223884 14-15 22.73010859522252 25.22505698840624 25.635572988662584 26.409261427708653 16-17 22.2591191509066 28.405280222128564 26.61703831692283 22.718562310042007 18-19 22.259389240470064 26.86406519702858 28.82917314077314 22.047372421728213 20-21 22.698022774990545 26.01052296067238 29.270289506621094 22.021164757715983 22-23 22.645034485890513 25.77709437432863 29.10060814512977 22.47726299465109 24-25 22.50279592802528 26.167148747615236 29.01271377973392 22.317341544625556 26-27 22.088661812137005 26.41223259972034 28.980642159696878 22.518463428445777 28-29 22.28589146154966 26.697136221234775 28.858817841837038 22.15815447537853 30-31 22.312536195915158 26.35374579575625 28.841074970666586 22.492643037662003 32-33 22.13361367399194 26.252567183930264 28.875328338527474 22.73849080355032 34-35 22.64163797174191 26.432424115409308 28.691479596980002 22.23445831586878 36-37 22.696904561467694 26.065647904716165 28.693588573443957 22.54385896037218 38-39 22.271901566565226 26.466378904210753 28.620183268183574 22.641536261040443 40-41 22.730054644808742 26.272735954162023 28.630823916388138 22.366385484641093 42-43 22.39193310466434 26.19423593707988 28.577158382809547 22.83667257544623 44-45 22.461771824830095 26.53051696792394 28.58479463165181 22.42291657559415 46-47 22.716174244900003 26.69494834431015 27.9825420660069 22.60633534478295 48-49 22.556409485927126 26.622022066375685 28.02780281847953 22.79376562921766 50-51 22.714572510271296 26.856197582425047 27.922226749516142 22.507003157787512 52-53 22.835313929538952 26.724307162722983 27.765316592971296 22.67506231476677 54-55 22.636532585796243 26.798028950296548 27.816936604720667 22.748501859186543 56-57 22.62156977689409 27.00517916093433 27.760531830763984 22.612719231407592 58-59 22.691454337018047 26.72489162681716 27.729377256691244 22.854276779473548 60-61 22.544294691003547 26.987472915990228 27.70665019873986 22.76158219426636 62-63 22.49526523492776 26.942622691596412 27.912541147299958 22.64957092617587 64-65 22.329084047561437 26.877432938969047 27.8115966022694 22.981886411200115 66-67 22.36210900118364 26.897709066297494 27.64948966911848 23.090692263400392 68-69 22.49456052202007 26.774967999949055 27.86906194742864 22.861409530602238 70-71 22.501680420968004 26.70874988353255 27.642081264245377 23.147488431254068 72-73 22.486175522530974 26.81270589223985 27.488581025115483 23.21253756011369 74-75 22.43878160673145 27.124881808042467 27.276952807052357 23.159383778173726 76-77 22.50401205069118 26.81501649923346 27.51295731594074 23.16801413413462 78-79 22.4577901823476 26.859608245685195 27.59093447547073 23.091667096496472 80-81 22.57710225778663 26.962556137990635 27.365202500006898 23.09513910421584 82-83 22.574873879027884 26.977841131623276 27.305431931824092 23.14185305752475 84-85 22.571004730924805 26.967948191396037 27.3324940818336 23.128552995845556 86-87 22.602805588251215 26.90923602539188 27.273960375337907 23.213998011018997 88-89 22.53286691348911 27.031366175590048 27.065156922146926 23.370609988773914 90-91 22.510549047152367 26.918829170919928 27.16176663187641 23.408855150051295 92-93 22.467931594476802 26.68400386563501 27.273888900563055 23.57417563932513 94-95 22.351588099499523 26.73049499533183 27.261067712064076 23.65684919310457 96-97 22.48713834555827 26.541254923933717 27.193272887802227 23.778333842705788 98-99 22.28475742465515 26.64624291809879 27.022402100243326 24.046597557002737 100-101 22.84105702496633 26.012836528033688 26.998687993249384 24.147418453750593 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 561.0 1 386.0 2 268.5 3 330.0 4 280.0 5 281.0 6 582.0 7 1327.0 8 2227.0 9 2679.5 10 2362.5 11 1952.5 12 2022.5 13 2172.0 14 2314.5 15 2474.5 16 2607.0 17 2768.5 18 2981.5 19 3261.0 20 3743.0 21 4447.5 22 5403.0 23 6639.5 24 8306.5 25 10615.0 26 13579.5 27 16741.5 28 19190.0 29 21769.0 30 25116.0 31 29539.5 32 35296.5 33 38673.0 34 41027.0 35 45023.0 36 49954.0 37 55311.5 38 59101.0 39 61776.0 40 64736.0 41 69726.5 42 73497.5 43 75617.5 44 78951.5 45 82757.0 46 85781.5 47 88449.0 48 88515.0 49 87080.0 50 87828.5 51 87082.5 52 85659.0 53 86923.5 54 88881.5 55 84238.0 56 75580.5 57 68753.5 58 63292.5 59 55605.5 60 44439.5 61 35577.5 62 29149.0 63 22169.5 64 16318.5 65 12315.0 66 8865.5 67 6684.0 68 5015.0 69 3555.5 70 2593.0 71 1747.5 72 1218.5 73 934.0 74 693.0 75 478.5 76 305.5 77 151.5 78 83.0 79 49.0 80 26.5 81 17.0 82 12.0 83 9.0 84 4.0 85 1.5 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007851688813380636 2 0.013835948936011283 3 0.009676675942977216 4 8.488312230681769E-5 5 0.001061039028835221 6 0.0019947533742102155 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001061039028835221 14-15 0.0 16-17 0.0028860261584318014 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003225558647659072 26-27 0.018801611590960117 28-29 0.007130182273772685 30-31 0.003374104111696003 32-33 0.0 34-35 0.009273481112019833 36-37 8.700520036448812E-4 38-39 0.0030133508418920276 40-41 0.0023767274245908953 42-43 0.0035014287951562295 44-45 0.0 46-47 1.273246834602265E-4 48-49 7.215065396079504E-4 50-51 0.004286597676494293 52-53 0.008424649888951655 54-55 0.007533377104730069 56-57 0.016721975094443085 58-59 0.02323675473149134 60-61 0.0036499742591931603 62-63 0.019841429839218633 64-65 0.020838806526323743 66-67 0.013050780054673219 68-69 0.030388157785840732 70-71 0.015809481529644794 72-73 0.005283974363599401 74-75 0.015851923090798203 76-77 0.019756546716911818 78-79 0.025358832789161782 80-81 0.011353117608536864 82-83 0.03410179438676401 84-85 0.021666416968815213 86-87 0.029857638271423122 88-89 0.02149665072420158 90-91 0.02701405367414473 92-93 0.023448962537258384 94-95 0.01402693596120162 96-97 0.014472572353312415 98-99 0.01118335136392323 100-101 0.010525507166045393 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2356181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.30770197581297 #Duplication Level Percentage of deduplicated Percentage of total 1 72.98656791655644 37.447730749001096 2 14.577862960456079 14.959132964386463 3 5.290847038526939 8.143836091570584 4 2.405821775353102 4.93748746626953 5 1.2811007769169136 3.2865168431517726 6 0.7663851211200129 2.359287563587377 7 0.5196396506782269 1.8663061432269819 8 0.34618738984607555 1.420966354080562 9 0.26854044710573116 1.2400373905697177 >10 1.3925173107451032 13.163464190185719 >50 0.10888398652551996 3.8159736283551537 >100 0.05166921575316896 4.84109532968255 >500 0.0026509402777369815 0.9039807460751006 >1k 0.0011597863715096428 0.9421495776517228 >5k 1.656837673585204E-4 0.6720349622056929 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8390 0.3560846980771002 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7421 0.314958825319447 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4371 0.18551206380155005 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6976624461363538E-4 2 0.0 0.0 0.0 0.0 1.6976624461363538E-4 3 0.0 0.0 0.0 0.0 1.6976624461363538E-4 4 0.0 0.0 0.0 0.0 1.6976624461363538E-4 5 0.0 0.0 0.0 4.2441561153408845E-5 2.1220780576704422E-4 6 0.0 0.0 0.0 4.2441561153408845E-5 2.1220780576704422E-4 7 0.0 0.0 0.0 4.2441561153408845E-5 2.1220780576704422E-4 8 0.0 0.0 0.0 4.2441561153408845E-5 2.1220780576704422E-4 9 0.0 0.0 0.0 4.2441561153408845E-5 2.546493669204531E-4 10-11 0.0 0.0 0.0 4.2441561153408845E-5 2.970909280738619E-4 12-13 0.0 0.0 0.0 4.2441561153408845E-5 4.880779532642017E-4 14-15 0.0 0.0 0.0 4.2441561153408845E-5 6.366234173011327E-4 16-17 0.0 0.0 0.0 8.488312230681769E-5 7.639481007613592E-4 18-19 0.0 0.0 0.0 8.488312230681769E-5 8.06389661914768E-4 20-21 0.0 0.0 0.0 8.488312230681769E-5 9.761559065284034E-4 22-23 0.0 0.0 0.0 1.4854546403693096E-4 0.0010610390288352213 24-25 0.0 0.0 0.0 4.03194830957384E-4 0.00110348058998863 26-27 0.0 0.0 0.0 0.001061039028835221 0.00110348058998863 28-29 0.0 0.0 0.0 0.00422293533476418 0.00110348058998863 30-31 0.0 0.0 0.0 0.013432754105053898 0.0011247013705653344 32-33 0.0 0.0 0.0 0.03234046959889754 0.0012308052734488564 34-35 0.0 0.0 0.0 0.05895132844208488 0.0012308052734488564 36-37 0.0 0.0 0.0 0.10419403263161871 0.0012308052734488564 38-39 0.0 0.0 0.0 0.1699572316388257 0.001315688395755674 40-41 0.0 0.0 0.0 0.23975237895560653 0.001358129956909083 42-43 0.0 0.0 0.0 0.31315505897042717 0.0014430130792159007 44-45 0.0 0.0 0.0 0.4016457139752846 0.0015278962015227182 46-47 0.0 0.0 0.0 0.503208369815392 0.0016340001044062404 48-49 0.0 0.0 0.0 0.6084422206952692 0.0016552208849829448 50-51 0.0 0.0 0.0 0.7205728252625754 0.0016976624461363537 52-53 0.0 0.0 0.0 0.8270587021964781 0.0017613247878664668 54-55 0.0 0.0 0.0 0.9453008915698752 0.0018037663490198758 56-57 0.0 0.0 0.0 1.0818141730198145 0.0018249871295965801 58-59 0.0 0.0 0.0 1.224672467862189 0.0018249871295965801 60-61 0.0 0.0 0.0 1.3754885554208274 0.001867428690749989 62-63 0.0 0.0 0.0 1.519874746464724 0.0020371949353636246 64-65 0.0 0.0 0.0 1.675974808386962 0.0020371949353636246 66-67 0.0 0.0 0.0 1.8541232613283953 0.002058415715940329 68-69 0.0 0.0 0.0 2.040038519960903 0.0020796364965170334 70-71 0.0 0.0 0.0 2.238537701475396 0.002164519618823851 72-73 0.0 2.1220780576704422E-5 0.0 2.4394348311950567 0.0022918443022840775 74-75 0.0 4.2441561153408845E-5 0.0 2.6476319094331036 0.0023767274245908953 76-77 0.0 4.2441561153408845E-5 0.0 2.8668213520098837 0.0023767274245908953 78-79 0.0 4.2441561153408845E-5 0.0 3.113067289821962 0.002419168985744304 80-81 0.0 4.2441561153408845E-5 0.0 3.366422189127236 0.0025040521080511215 82-83 0.0 4.2441561153408845E-5 0.0 3.6243607770370785 0.0025889352303579394 84-85 0.0 4.2441561153408845E-5 0.0 3.888920248486852 0.002631376791511348 86-87 0.0 4.2441561153408845E-5 0.0 4.160376473624055 0.0026525975720880527 88-89 0.0 4.2441561153408845E-5 0.0 4.4569368821835 0.0026950391332414615 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4365 0.0 53.98018 1 GTATCAA 7380 0.0 41.13215 1 ATCAACG 9225 0.0 32.642006 3 TATCAAC 9310 0.0 32.24469 2 TCAACGC 9345 0.0 32.067646 4 CAACGCA 9535 0.0 31.478456 5 AACGCAG 9920 0.0 30.256763 6 GGACCGA 710 0.0 28.762535 6 TGGTATC 1980 0.0 27.828104 2 ACGCAGA 11380 0.0 26.082281 7 CGCAGAG 11370 0.0 26.021685 8 AGGACCG 895 0.0 26.001003 5 GTGGTAT 2290 0.0 24.685865 1 GCAGAGT 12385 0.0 23.62069 9 ACCGAGT 900 0.0 22.689964 8 GAGTACT 7900 0.0 22.663727 12-13 CAGAGTA 11850 0.0 21.38125 10-11 GTACTTT 8265 0.0 21.288898 14-15 GTACATG 7315 0.0 20.846233 1 TACATGG 7110 0.0 20.442907 2 >>END_MODULE