FastQCFastQC Report
Thu 26 May 2016
SRR938131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767397
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81200.45943271375927425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70120.39674164887685104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41260.23345066218851793No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.10869091664181844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.058.3079261
GTATCAA62850.041.7321781
TGGTATC13850.032.946252
TATCAAC81150.032.156472
ATCAACG81000.032.0986563
TCAACGC82650.031.5153624
CAACGCA84750.030.6222785
GTGGTAT15600.029.9581581
AACGCAG88650.029.3229066
ACGCAGA99150.026.1600487
CGCAGAG99750.026.0026958
GCAGAGT106950.023.3167489
GAGTACT68000.023.01567512-13
CAGAGTA102400.021.84762810-11
TACCTGG14050.021.651512
GTGTAAG7300.021.5578141
GTACTTT73150.021.16892814-15
GTACCTG14450.021.121551
GTACATG59800.020.6543831
AGAGTAC97000.020.61549210-11
Produced by FastQC (version 0.11.5)