FastQCFastQC Report
Thu 26 May 2016
SRR938131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767397
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77230.43697030152252153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71920.40692611790107147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.2446535781151603No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.11055806929625885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.054.5732351
GTATCAA63400.043.2793661
GTGGTAT16250.035.9953231
TGGTATC16200.034.6386832
ATCAACG82300.033.0495833
TATCAAC83050.032.8102342
TCAACGC83200.032.6911474
CAACGCA85150.031.9415915
AACGCAG89100.030.5967466
ACGCAGA101200.026.844587
CGCAGAG101450.026.7316138
ACCGTCG908.955778E-426.3856988
GCAGAGT111300.024.1951899
GTACCTG14700.023.615651
GAGTACT74500.022.66336812-13
GTACATG59250.022.4731541
TACATGG58650.022.05442
GTATAGG4550.021.9483681
TACCTGG15000.021.5581742
CAGAGTA106150.021.45407110-11
Produced by FastQC (version 0.11.5)