##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938131_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1767397 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.454907414689515 33.0 31.0 34.0 31.0 34.0 2 32.68162501124535 34.0 31.0 34.0 31.0 34.0 3 32.74944225886996 34.0 31.0 34.0 31.0 34.0 4 36.215668013468395 37.0 35.0 37.0 35.0 37.0 5 36.14007605535146 37.0 35.0 37.0 35.0 37.0 6 36.19305736062696 37.0 37.0 37.0 35.0 37.0 7 36.16589028950485 37.0 37.0 37.0 35.0 37.0 8 36.15559662034053 37.0 36.0 37.0 35.0 37.0 9 37.84482942994698 39.0 38.0 39.0 35.0 39.0 10-11 37.81480844428275 39.0 38.0 39.0 35.0 39.0 12-13 37.73307468554037 39.0 38.0 39.0 35.0 39.0 14-15 38.96320888855192 40.0 38.0 41.0 35.5 41.0 16-17 38.85804830493658 40.0 38.0 41.0 35.0 41.0 18-19 39.11207216035786 40.0 38.5 41.0 36.0 41.0 20-21 39.08605876325466 40.0 39.0 41.0 36.0 41.0 22-23 38.97704392391749 40.0 38.5 41.0 35.0 41.0 24-25 38.87126802863194 40.0 38.0 41.0 35.0 41.0 26-27 38.70463653610366 40.0 38.0 41.0 34.5 41.0 28-29 38.573493391694115 40.0 38.0 41.0 34.0 41.0 30-31 38.32815236191982 40.0 38.0 41.0 34.0 41.0 32-33 38.09025872511948 40.0 37.5 41.0 33.5 41.0 34-35 37.99735628158246 40.0 37.5 41.0 33.0 41.0 36-37 37.94558551361126 40.0 37.5 41.0 33.0 41.0 38-39 37.75410080474279 40.0 37.0 41.0 33.0 41.0 40-41 37.52779369886902 40.0 37.0 41.0 33.0 41.0 42-43 37.26189192354632 40.0 36.5 41.0 32.0 41.0 44-45 36.97940841814261 40.0 36.0 41.0 31.0 41.0 46-47 36.603320023741134 39.0 36.0 41.0 30.5 41.0 48-49 36.17780130893059 39.0 35.0 41.0 29.5 41.0 50-51 35.78508422272981 39.0 35.0 40.0 28.5 41.0 52-53 35.23540806055459 38.0 34.5 40.0 27.0 41.0 54-55 34.84147845673609 38.0 34.0 40.0 26.0 41.0 56-57 34.44577138017095 38.0 34.0 40.0 25.0 41.0 58-59 33.917633106766615 37.5 33.0 40.0 23.5 41.0 60-61 33.42459758616768 37.0 32.5 39.5 22.0 41.0 62-63 32.8476018687369 36.0 32.0 39.0 20.5 40.0 64-65 32.252776257965806 35.5 31.0 39.0 18.0 40.0 66-67 31.67113019881781 35.0 31.0 38.0 13.0 40.0 68-69 31.024630289629325 35.0 30.0 38.0 8.0 40.0 70-71 30.395364765245162 34.5 29.0 37.0 6.5 39.5 72-73 29.7090896951845 34.0 28.5 36.5 2.0 39.0 74-75 29.036757729021833 34.0 27.5 36.0 2.0 39.0 76-77 27.76287189578799 31.5 26.0 34.5 2.0 36.5 78-79 28.404341808886176 33.5 27.0 35.0 2.0 37.0 80-81 28.12182209203705 33.5 27.0 35.0 2.0 37.0 82-83 27.557388068441895 33.0 26.0 35.0 2.0 36.0 84-85 26.889703332075364 33.0 25.0 35.0 2.0 36.0 86-87 26.15189343424256 32.0 23.5 35.0 2.0 35.5 88-89 25.331400924636625 32.0 20.0 34.0 2.0 35.0 90-91 24.667347234379147 31.0 17.5 34.0 2.0 35.0 92-93 23.72556901477144 31.0 4.5 34.0 2.0 35.0 94-95 22.81480816138083 30.5 2.0 34.0 2.0 35.0 96-97 21.755411772227745 30.0 2.0 34.0 2.0 35.0 98-99 20.342070287547166 29.0 2.0 34.0 2.0 35.0 100-101 16.567404493727217 14.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10852768393061751 1101 2 0.08274221933849901 1101 3 0.10856712146186709 1101 4 0.025356635672089567 1101 5 0.024283009257118238 1101 6 0.007843940615941847 1101 7 0.024669654768452176 1101 8 0.09065003164978691 1101 9 -0.09975257516872915 1101 10-11 0.0892616539576494 1101 12-13 0.17500308585798763 1101 14-15 0.28554868512603804 1101 16-17 0.3272969699528119 1101 18-19 0.19440386336023607 1101 20-21 0.13060143297909832 1101 22-23 0.11674638779420121 1101 24-25 0.20327264876137718 1101 26-27 0.15109792878782002 1101 28-29 0.22901973950722265 1101 30-31 0.1679770271066019 1101 32-33 0.3535541253543286 1101 34-35 0.34071675455749073 1101 36-37 0.3709588876175829 1101 38-39 0.45931839533870544 1101 40-41 0.4445146279693688 1101 42-43 0.6120324752207793 1101 44-45 0.6542116523718491 1101 46-47 0.45815191703435687 1101 48-49 0.6455768537149496 1101 50-51 0.6950739924929579 1101 52-53 0.43888481455583417 1101 54-55 0.7241626574707638 1101 56-57 0.7970417017987543 1101 58-59 0.9925681782533715 1101 60-61 0.8291576752559777 1101 62-63 0.8497708019914683 1101 64-65 0.9751762792890943 1101 66-67 1.02674083274945 1101 68-69 1.20756171950811 1101 70-71 1.4306065534787926 1101 72-73 1.5964608954636823 1101 74-75 1.7399859412659637 1101 76-77 1.6717155902267002 1101 78-79 1.7634215570834186 1101 80-81 1.7613128487613672 1101 82-83 1.918404336200755 1101 84-85 2.0191611646075707 1101 86-87 2.2836890096810727 1101 88-89 2.654082768931442 1101 90-91 3.0398114484471677 1101 92-93 3.188213535041804 1101 94-95 3.299004342465029 1101 96-97 3.6797012761593706 1101 98-99 3.922937737271571 1101 100-101 3.9427891538457125 1102 1 0.16115474984318467 1102 2 0.1341674711717431 1102 3 0.1661558792700717 1102 4 0.09081846539249483 1102 5 -0.04243854788449397 1102 6 0.0465803343476523 1102 7 0.050781837850031764 1102 8 0.01625563011907616 1102 9 0.10579175188272671 1102 10-11 0.08034973341654705 1102 12-13 0.21359626752720828 1102 14-15 0.24787470940653833 1102 16-17 0.37462451614207026 1102 18-19 0.28359961314945537 1102 20-21 0.2721283968025645 1102 22-23 0.25713281793500187 1102 24-25 0.2277152216221907 1102 26-27 0.31347010416558163 1102 28-29 0.1704509442932931 1102 30-31 0.2167838326874758 1102 32-33 0.18359238407522582 1102 34-35 0.32641844428025735 1102 36-37 0.3954077456472902 1102 38-39 0.47988279452594185 1102 40-41 0.7130924648476906 1102 42-43 0.7136953064379554 1102 44-45 0.5279455275465992 1102 46-47 0.5742201308701667 1102 48-49 0.6938956608258096 1102 50-51 0.8954564001256671 1102 52-53 0.872500636301254 1102 54-55 1.089189553504191 1102 56-57 1.1075658579474918 1102 58-59 1.1397987083714511 1102 60-61 0.9498921780497369 1102 62-63 1.2114827203776528 1102 64-65 1.0229224626202864 1102 66-67 1.0961501974715127 1102 68-69 1.4255298052162608 1102 70-71 1.6979410492690796 1102 72-73 1.8651096649622971 1102 74-75 1.9472676685740353 1102 76-77 1.805374420484938 1102 78-79 1.8943894371767662 1102 80-81 2.0173849953171406 1102 82-83 2.2131181500142993 1102 84-85 2.285484870558637 1102 86-87 2.215164731420483 1102 88-89 2.565350774817947 1102 90-91 2.6798275025642653 1102 92-93 2.608980900290028 1102 94-95 3.0058068256454646 1102 96-97 3.0721306106587534 1102 98-99 3.456724111564153 1102 100-101 3.4433312496622115 1103 1 0.19779674014282023 1103 2 0.16733740693960186 1103 3 0.18953920926875867 1103 4 0.17431163167165664 1103 5 0.007233564601108355 1103 6 0.06920560773097861 1103 7 0.12367601004186213 1103 8 0.09633711042512516 1103 9 0.19440417193750648 1103 10-11 0.19041627681637152 1103 12-13 0.24638243174348418 1103 14-15 0.3614361611496406 1103 16-17 0.32110132081701437 1103 18-19 0.28452497691313994 1103 20-21 0.17321063257800517 1103 22-23 0.3143699732305336 1103 24-25 0.31807962349051166 1103 26-27 0.29335875004706935 1103 28-29 0.36330218581581875 1103 30-31 0.31071213553755683 1103 32-33 0.3323775961930906 1103 34-35 0.32070585381365646 1103 36-37 0.3485138804727086 1103 38-39 0.5310102204795939 1103 40-41 0.6172422858117983 1103 42-43 0.6800053386575158 1103 44-45 0.4523860834892872 1103 46-47 0.3662819241268309 1103 48-49 0.5638876576797429 1103 50-51 0.5059015406291749 1103 52-53 0.6293589357707035 1103 54-55 0.7519781152146621 1103 56-57 0.8768018679652272 1103 58-59 0.9052371048445522 1103 60-61 0.9356628775660951 1103 62-63 1.1713302983910765 1103 64-65 1.0782442097898652 1103 66-67 1.2517178171564929 1103 68-69 1.2173337384865768 1103 70-71 1.3465705967463606 1103 72-73 1.7206645210091551 1103 74-75 1.8389621622715566 1103 76-77 1.7096242807748467 1103 78-79 1.7284147091802389 1103 80-81 1.9684420487795329 1103 82-83 1.9433487406602765 1103 84-85 2.256450181060007 1103 86-87 2.4594344615902735 1103 88-89 2.6518908284942206 1103 90-91 3.031410538654317 1103 92-93 3.0650218094750166 1103 94-95 3.262569829033005 1103 96-97 3.338368459330301 1103 98-99 4.017908939393028 1103 100-101 3.811250017729659 1104 1 0.1579241524263395 1104 2 0.13472256231573 1104 3 0.20283650031132794 1104 4 0.07513961866042962 1104 5 0.05106126873718608 1104 6 0.027123233311151296 1104 7 0.11296939257442062 1104 8 0.21336419999943246 1104 9 0.15154877395090693 1104 10-11 0.22695409550778578 1104 12-13 0.1830427127962082 1104 14-15 0.3301929913994499 1104 16-17 0.33407477766268556 1104 18-19 0.30859317012725285 1104 20-21 0.3154317012469292 1104 22-23 0.29942217241026015 1104 24-25 0.37285420917559264 1104 26-27 0.407102498359599 1104 28-29 0.3515163888783377 1104 30-31 0.3483090447289925 1104 32-33 0.533374973377164 1104 34-35 0.512637480602379 1104 36-37 0.5036368892539969 1104 38-39 0.529842649541429 1104 40-41 0.5737625226366418 1104 42-43 0.6657084204662311 1104 44-45 0.7553912326940804 1104 46-47 0.6502684436243769 1104 48-49 0.7478576238659898 1104 50-51 0.9586137122950475 1104 52-53 1.0625309680673354 1104 54-55 1.1049051585039749 1104 56-57 1.2560510869060693 1104 58-59 1.416415411328309 1104 60-61 1.32697913902485 1104 62-63 1.4827888861847 1104 64-65 1.6705924014611426 1104 66-67 1.7405523676135566 1104 68-69 1.7154976066257923 1104 70-71 1.8860132805041907 1104 72-73 2.1745217639667658 1104 74-75 2.0278595510268786 1104 76-77 2.09325691297634 1104 78-79 2.14132982765695 1104 80-81 2.359573491714002 1104 82-83 2.5654808392987256 1104 84-85 2.6722677539267217 1104 86-87 2.864901393538485 1104 88-89 2.99721540196521 1104 90-91 3.3326229481735936 1104 92-93 3.3942519037163947 1104 94-95 3.6218506117635627 1104 96-97 4.14329089983481 1104 98-99 4.482699403400019 1104 100-101 4.1020563407731565 1105 1 0.10612419875369028 1105 2 0.14730513021329728 1105 3 0.16318119999354508 1105 4 0.07880682177556508 1105 5 0.0020723706467506986 1105 6 -0.05155036630853971 1105 7 0.11861004981715695 1105 8 0.07239432337249241 1105 9 0.17408263757591413 1105 10-11 0.16141187630676512 1105 12-13 0.2323822831630551 1105 14-15 0.3772135449961027 1105 16-17 0.366567128351619 1105 18-19 0.21362721921303773 1105 20-21 0.189574039748436 1105 22-23 0.15849922871615263 1105 24-25 0.18455739016867057 1105 26-27 0.2408268763846806 1105 28-29 0.2857497242807625 1105 30-31 0.30276782474524566 1105 32-33 0.34799632301692185 1105 34-35 0.47599610725393404 1105 36-37 0.3675762352511498 1105 38-39 0.38160687362650236 1105 40-41 0.4248142366727734 1105 42-43 0.4930196403137117 1105 44-45 0.6442194157688519 1105 46-47 0.6011202006571281 1105 48-49 0.5731217730097597 1105 50-51 0.8053788220499811 1105 52-53 0.8129219496771967 1105 54-55 1.0550345647115407 1105 56-57 0.9335188886431922 1105 58-59 0.9400130250157659 1105 60-61 0.9067416583846821 1105 62-63 1.0864665735114372 1105 64-65 1.3368057616017595 1105 66-67 1.4234026954628014 1105 68-69 1.6368041362079317 1105 70-71 1.8674159166210451 1105 72-73 1.73180113754837 1105 74-75 1.692704097041588 1105 76-77 1.7765409919629498 1105 78-79 1.7734997793765892 1105 80-81 1.924161723356793 1105 82-83 2.068382922061172 1105 84-85 2.4050460170145733 1105 86-87 2.447956031042139 1105 88-89 2.4239048361508573 1105 90-91 2.4840149638219415 1105 92-93 2.892761972344452 1105 94-95 3.0154971795279053 1105 96-97 3.235300075980163 1105 98-99 3.427435117999341 1105 100-101 3.209704781312915 1106 1 0.06891034722348621 1106 2 0.024780734998749665 1106 3 0.006743699565589623 1106 4 0.06925163148802937 1106 5 0.02041837924475942 1106 6 0.06502158574468808 1106 7 0.12108227601360255 1106 8 0.138261492749173 1106 9 0.09649798094687156 1106 10-11 0.13878451971840633 1106 12-13 0.10129591273347671 1106 14-15 0.05830879886237739 1106 16-17 0.10564293379822942 1106 18-19 0.04075644204387174 1106 20-21 0.04529459457941698 1106 22-23 -0.02480589264816757 1106 24-25 -0.05854299050371736 1106 26-27 -0.12661116569865527 1106 28-29 -0.04740545980818922 1106 30-31 0.03086889194691622 1106 32-33 0.023329596046188783 1106 34-35 0.010176976908141455 1106 36-37 0.02784820896337692 1106 38-39 -0.06902049881870198 1106 40-41 0.05847680374451869 1106 42-43 -0.034593252309868205 1106 44-45 0.06127910994077723 1106 46-47 0.11473276100773688 1106 48-49 0.1437960889220662 1106 50-51 0.26992147642086906 1106 52-53 0.2965371444262743 1106 54-55 0.2937925125043179 1106 56-57 0.2516855707462611 1106 58-59 0.5806383685056531 1106 60-61 0.4422728824934268 1106 62-63 0.5104221930870025 1106 64-65 0.536080347008344 1106 66-67 0.7314413587740667 1106 68-69 0.7734209415078617 1106 70-71 0.7501932933728241 1106 72-73 0.9161890291178274 1106 74-75 0.9956921752172754 1106 76-77 0.9158840933526982 1106 78-79 1.114678706927748 1106 80-81 0.9240036954869453 1106 82-83 1.483832605951374 1106 84-85 1.6330301399887297 1106 86-87 1.9135603732227473 1106 88-89 1.8991834751434844 1106 90-91 1.808047957590567 1106 92-93 1.6445023511140135 1106 94-95 2.069907418436493 1106 96-97 2.4152395821187405 1106 98-99 2.334008032274717 1106 100-101 2.6663253201789416 1107 1 0.26417890449367576 1107 2 0.25962015342280864 1107 3 0.22164879291490536 1107 4 0.09811752191571088 1107 5 0.1347227034636731 1107 6 0.14359824554127698 1107 7 0.16957277693046535 1107 8 0.14341229534137767 1107 9 0.17222652926585624 1107 10-11 0.22410580212619635 1107 12-13 0.3561306871925112 1107 14-15 0.4798433276542582 1107 16-17 0.3594191367745694 1107 18-19 0.28717718667770953 1107 20-21 0.3213213177219387 1107 22-23 0.37676151569664995 1107 24-25 0.41086927433417486 1107 26-27 0.3955811295284377 1107 28-29 0.3934141509124913 1107 30-31 0.4556652449612102 1107 32-33 0.46643418487289523 1107 34-35 0.37260547598145877 1107 36-37 0.5099656114857112 1107 38-39 0.5345600894075133 1107 40-41 0.5626758390344406 1107 42-43 0.6167584431582682 1107 44-45 0.6109563824320858 1107 46-47 0.8054680157172882 1107 48-49 0.896781520060209 1107 50-51 0.957977156298746 1107 52-53 0.9117246638053587 1107 54-55 1.0891879720814828 1107 56-57 1.1839920640195203 1107 58-59 1.1208714506533184 1107 60-61 1.1097079897465676 1107 62-63 1.2217876661190417 1107 64-65 1.3800915261966438 1107 66-67 1.5638962905667064 1107 68-69 1.8236075068397533 1107 70-71 1.834182592611132 1107 72-73 1.9899507319798282 1107 74-75 2.120385617405521 1107 76-77 2.0949831290635252 1107 78-79 2.112432837741647 1107 80-81 2.151414523642 1107 82-83 2.5265956669276157 1107 84-85 2.560765098663051 1107 86-87 2.693486588896345 1107 88-89 2.7345970054969193 1107 90-91 2.825210459887991 1107 92-93 3.251924908437516 1107 94-95 3.301976066341169 1107 96-97 3.144265626204138 1107 98-99 3.5354259849174987 1107 100-101 3.283664882411582 1108 1 0.21885008197050837 1108 2 0.2096331736754138 1108 3 0.12800404295410317 1108 4 0.08692621915670884 1108 5 -0.0074760791067447485 1108 6 0.11822637705589756 1108 7 0.11163588990741147 1108 8 0.1379455876237472 1108 9 0.15149015844905733 1108 10-11 0.1521167535254122 1108 12-13 0.2101157477115123 1108 14-15 0.3282660067762535 1108 16-17 0.19821869825822347 1108 18-19 0.2481288376601185 1108 20-21 0.3067737031617028 1108 22-23 0.24611137348096435 1108 24-25 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1.713610734649187 2316 74-75 2.05249420070205 2316 76-77 2.084759939047366 2316 78-79 2.1873092170469413 2316 80-81 2.2561909972429994 2316 82-83 2.4263763670162675 2316 84-85 2.6607387577162527 2316 86-87 2.792333501064615 2316 88-89 3.024007239565119 2316 90-91 3.203625830847738 2316 92-93 3.3362086137270843 2316 94-95 3.747558952701276 2316 96-97 4.2240203705586055 2316 98-99 4.470134888971344 2316 100-101 4.892582255934347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 14.0 5 29.0 6 73.0 7 189.0 8 471.0 9 891.0 10 1517.0 11 2527.0 12 3978.0 13 5980.0 14 7925.0 15 11248.0 16 14651.0 17 19540.0 18 20530.0 19 17662.0 20 16959.0 21 18395.0 22 19836.0 23 21547.0 24 24044.0 25 26368.0 26 30437.0 27 35218.0 28 41406.0 29 50066.0 30 58582.0 31 70166.0 32 83793.0 33 104504.0 34 135240.0 35 184263.0 36 253212.0 37 312897.0 38 165669.0 39 7564.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98513576745915 18.802623292899106 15.16020452677016 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22.66702389871057 44-45 22.294201872269348 26.40534074040334 28.98741343180255 22.313043955524762 46-47 22.590538515115732 26.64115645777378 28.34979916792888 22.418505859181607 48-49 22.534523935482518 26.586443226960327 28.290729247588402 22.58830358996875 50-51 22.59243395796191 26.931159213238452 28.096799983252208 22.379606845547436 52-53 22.805374231143315 26.745179492779492 27.99062689367471 22.45881938240248 54-55 22.60830475552465 26.860575184862256 28.048310594620222 22.48280946499287 56-57 22.52157339906467 26.98211748184406 28.02149942019236 22.474809698898916 58-59 22.65905735949535 26.661695136972625 27.920495508366262 22.75875199516577 60-61 22.384048405649665 26.88397117342623 28.084804941957014 22.647175478967092 62-63 22.439497181448196 26.89274113286375 28.213808216263804 22.453953469424242 64-65 22.229442507823652 26.932177660140873 28.11445306289419 22.723926769141283 66-67 22.237432923917446 26.929731650405454 27.933213609174175 22.899621816502925 68-69 22.44920707129003 26.726942601783893 28.102431180306127 22.721419146619954 70-71 22.458395029526475 26.765180658335392 27.73839154417485 23.038032767963283 72-73 22.46050756607675 26.88314528355425 27.674507488561556 22.981839661807445 74-75 22.362066926672387 27.24699091375622 27.526243396361995 22.864698763209397 76-77 22.437431997451622 26.913704164938608 27.76141976024628 22.88744407736349 78-79 22.480772571188023 26.989550168977317 27.80190302461756 22.7277742352171 80-81 22.516276761814126 27.09122511806912 27.58647321456357 22.806024905553198 82-83 22.522410633303025 27.09871768684581 27.59505316708094 22.783818512770225 84-85 22.565329401187 27.085694497805424 27.552221932391152 22.796754168616427 86-87 22.71127413050951 27.134299095959047 27.36577658263749 22.788650190893954 88-89 22.649070921813266 27.20656422976841 27.16642044769794 22.977944400720382 90-91 22.554923028262294 27.111354540758214 27.31717267278397 23.016549758195524 92-93 22.549772348827116 26.914524580498895 27.34979747051738 23.185905600156616 94-95 22.44264374179761 26.90799715853234 27.369014253451336 23.280344846218718 96-97 22.640552904132917 26.72096503611247 27.266768322870664 23.371713736883944 98-99 22.446994082257692 26.762012156861193 27.10081549306692 23.690178267814193 100-101 22.98985457144037 26.195387906622 27.155019500429162 23.659738021508467 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 771.0 1 539.0 2 436.0 3 574.0 4 450.0 5 383.0 6 767.0 7 1741.5 8 2852.5 9 3382.0 10 2762.5 11 1857.5 12 1719.5 13 1870.0 14 1971.0 15 2119.0 16 2286.5 17 2415.5 18 2621.0 19 2870.5 20 3202.5 21 3738.0 22 4448.0 23 5494.0 24 6764.5 25 8408.5 26 10676.5 27 13081.5 28 15313.5 29 17477.0 30 19644.0 31 22673.5 32 26947.5 33 29742.5 34 31296.0 35 33560.5 36 36586.0 37 39841.0 38 42799.0 39 45409.5 40 48134.0 41 51245.0 42 53321.0 43 55593.5 44 58832.0 45 62079.0 46 63597.5 47 65333.5 48 66443.0 49 65895.5 50 65644.0 51 64293.0 52 62922.0 53 63044.5 54 63821.0 55 60496.5 56 55143.0 57 50818.5 58 46744.5 59 41626.5 60 34066.0 61 27891.5 62 23105.0 63 17407.0 64 12803.5 65 9677.5 66 7006.0 67 5338.0 68 4070.5 69 2956.5 70 2199.5 71 1500.0 72 1023.0 73 741.0 74 535.5 75 373.0 76 229.0 77 135.0 78 79.5 79 46.0 80 33.0 81 18.0 82 9.0 83 6.0 84 3.5 85 2.0 86 2.5 87 2.0 88 1.5 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0168043738899636 3 0.0022632153387156365 4 0.0021500545717798546 5 0.11287786501844238 6 5.6580383467890915E-5 7 0.0 8 2.8290191733945457E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.394823008073455E-4 24-25 5.940940264128546E-4 26-27 0.0064784539070735095 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011316076693578182 36-37 6.78964601614691E-4 38-39 0.007242289083890037 40-41 0.010806853242367164 42-43 0.005940940264128546 44-45 0.004328399335293655 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.8290191733945458E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.6580383467890915E-5 68-69 0.0014145095866972728 70-71 0.0 72-73 8.487057520183638E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0024895368725872005 84-85 0.004752752211302837 86-87 0.0030836308990000554 88-89 0.0 90-91 0.003394823008073455 92-93 0.0 94-95 2.8290191733945458E-5 96-97 1.1316076693578183E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1767397.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.602244487838384 #Duplication Level Percentage of deduplicated Percentage of total 1 80.56293135553396 49.62857394020544 2 11.743756135803366 14.468834733666222 3 3.281449691998692 6.064339986031361 4 1.404889574042476 3.4617740407431894 5 0.7691655071949093 2.3691160812916507 6 0.4756958642148064 1.7582359757528447 7 0.31403192151022946 1.3541549844101184 8 0.22603514546658643 1.1139417835101417 9 0.17429333167513117 0.9663174387406205 >10 0.961368711660238 10.631451478574528 >50 0.05587322970825056 2.339864956861755 >100 0.026742479479160357 3.0273888696788886 >500 0.002572620681534484 1.0203231022113486 >1k 0.001010672410602833 0.951497846079491 >5k 1.8375862010960598E-4 0.8441847822423179 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7723 0.43697030152252153 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7192 0.40692611790107147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4324 0.2446535781151603 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1954 0.11055806929625885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.6580383467890915E-5 0.0 4 0.0 0.0 0.0 5.6580383467890915E-5 0.0 5 0.0 0.0 0.0 5.6580383467890915E-5 0.0 6 0.0 0.0 0.0 5.6580383467890915E-5 0.0 7 0.0 0.0 0.0 5.6580383467890915E-5 0.0 8 0.0 0.0 0.0 5.6580383467890915E-5 0.0 9 0.0 0.0 0.0 5.6580383467890915E-5 0.0 10-11 0.0 0.0 0.0 5.6580383467890915E-5 0.0 12-13 0.0 0.0 0.0 5.6580383467890915E-5 5.6580383467890915E-5 14-15 0.0 0.0 0.0 5.6580383467890915E-5 8.487057520183638E-5 16-17 0.0 0.0 0.0 5.6580383467890915E-5 1.1316076693578183E-4 18-19 0.0 0.0 0.0 1.1316076693578183E-4 1.1316076693578183E-4 20-21 0.0 0.0 0.0 1.1316076693578183E-4 1.1316076693578183E-4 22-23 0.0 0.0 0.0 2.2632153387156366E-4 1.6974115040367275E-4 24-25 0.0 0.0 0.0 3.960626842752364E-4 1.6974115040367275E-4 26-27 0.0 0.0 0.0 7.355449850825819E-4 1.6974115040367275E-4 28-29 0.0 0.0 0.0 0.0028290191733945456 1.6974115040367275E-4 30-31 0.0 0.0 0.0 0.009675245573009347 1.6974115040367275E-4 32-33 0.0 0.0 0.0 0.02498023930107384 1.6974115040367275E-4 34-35 0.0 0.0 0.0 0.04667881636101001 1.6974115040367275E-4 36-37 0.0 0.0 0.0 0.08187181487803816 1.6974115040367275E-4 38-39 0.0 0.0 0.0 0.13494421457091982 1.980313421376182E-4 40-41 0.0 0.0 0.0 0.1912982765049392 2.2632153387156366E-4 42-43 0.0 0.0 0.0 0.2481332716984356 2.2632153387156366E-4 44-45 0.0 0.0 0.0 0.3145020615062717 2.2632153387156366E-4 46-47 0.0 0.0 0.0 0.39532713929015384 2.2632153387156366E-4 48-49 0.0 0.0 0.0 0.4817536750373572 2.2632153387156366E-4 50-51 0.0 0.0 0.0 0.5740645706652212 2.2632153387156366E-4 52-53 0.0 0.0 0.0 0.6595858202769384 2.2632153387156366E-4 54-55 0.0 0.0 0.0 0.7514157826453253 2.2632153387156366E-4 56-57 0.0 0.0 0.0 0.8638127144042906 2.2632153387156366E-4 58-59 0.0 0.0 0.0 0.9804248847316137 2.2632153387156366E-4 60-61 0.0 0.0 0.0 1.1056655635377903 2.2632153387156366E-4 62-63 0.0 0.0 0.0 1.2272850978020218 2.2632153387156366E-4 64-65 0.0 0.0 0.0 1.353233031401547 2.2632153387156366E-4 66-67 0.0 0.0 0.0 1.4993518717073755 2.2632153387156366E-4 68-69 0.0 0.0 0.0 1.656730208323314 2.2632153387156366E-4 70-71 2.8290191733945458E-5 0.0 0.0 1.8253680412493627 3.394823008073455E-4 72-73 5.6580383467890915E-5 0.0 0.0 1.988772188704632 3.394823008073455E-4 74-75 5.6580383467890915E-5 0.0 0.0 2.1573534412472126 3.394823008073455E-4 76-77 5.6580383467890915E-5 0.0 0.0 2.3373073508668396 3.394823008073455E-4 78-79 5.6580383467890915E-5 0.0 0.0 2.5367249123994213 3.394823008073455E-4 80-81 5.6580383467890915E-5 0.0 0.0 2.74709077813304 3.394823008073455E-4 82-83 5.6580383467890915E-5 0.0 0.0 2.96894246171064 3.960626842752364E-4 84-85 5.6580383467890915E-5 0.0 0.0 3.1945850309805888 3.960626842752364E-4 86-87 5.6580383467890915E-5 0.0 0.0 3.424216517285024 4.526430677431273E-4 88-89 5.6580383467890915E-5 0.0 0.0 3.6678799386894965 4.526430677431273E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3625 0.0 54.573235 1 GTATCAA 6340 0.0 43.279366 1 GTGGTAT 1625 0.0 35.995323 1 TGGTATC 1620 0.0 34.638683 2 ATCAACG 8230 0.0 33.049583 3 TATCAAC 8305 0.0 32.810234 2 TCAACGC 8320 0.0 32.691147 4 CAACGCA 8515 0.0 31.941591 5 AACGCAG 8910 0.0 30.596746 6 ACGCAGA 10120 0.0 26.84458 7 CGCAGAG 10145 0.0 26.731613 8 ACCGTCG 90 8.955778E-4 26.385698 8 GCAGAGT 11130 0.0 24.195189 9 GTACCTG 1470 0.0 23.61565 1 GAGTACT 7450 0.0 22.663368 12-13 GTACATG 5925 0.0 22.473154 1 TACATGG 5865 0.0 22.0544 2 GTATAGG 455 0.0 21.948368 1 TACCTGG 1500 0.0 21.558174 2 CAGAGTA 10615 0.0 21.454071 10-11 >>END_MODULE