FastQCFastQC Report
Thu 26 May 2016
SRR938130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751449
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79940.456422082515677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69040.39418789813463023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.24008692231403828No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18740.10699712066979969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35950.059.9954071
GTATCAA64700.042.9974481
TGGTATC13150.039.0288432
GTGGTAT15200.035.1603241
ATCAACG82100.033.456833
TATCAAC82950.033.2276232
TCAACGC83100.033.225784
CAACGCA84900.032.577325
AACGCAG87000.031.8446886
CGCAGAG97800.027.9298
ACGCAGA98400.027.8069767
GCAGAGT107600.024.8092279
GAGTACT71550.023.1711912-13
CAGAGTA101250.023.15161710-11
TACATGG56050.022.2980982
AGGACCG7150.021.9335045
GTACATG58500.021.8603651
GTACTTT75800.021.6222614-15
AGAGTAC97850.021.43181810-11
ACATGGG57600.021.2036573
Produced by FastQC (version 0.11.5)