##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938130_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1751449 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73494517967694 33.0 31.0 34.0 30.0 34.0 2 31.935608173575137 33.0 31.0 34.0 30.0 34.0 3 32.007429848085785 34.0 31.0 34.0 30.0 34.0 4 35.527723616274294 37.0 35.0 37.0 33.0 37.0 5 35.53404866484836 37.0 35.0 37.0 33.0 37.0 6 35.55116306555315 37.0 35.0 37.0 33.0 37.0 7 35.488295120211895 37.0 35.0 37.0 33.0 37.0 8 35.45740583939355 37.0 35.0 37.0 33.0 37.0 9 37.07785610657233 39.0 37.0 39.0 33.0 39.0 10-11 36.98033656703678 39.0 37.0 39.0 33.0 39.0 12-13 36.86694302831541 39.0 37.0 39.0 33.0 39.0 14-15 38.0257720892815 40.0 38.0 41.0 33.0 41.0 16-17 37.85785683739578 40.0 38.0 41.0 33.0 41.0 18-19 38.002534758362934 40.0 38.0 41.0 33.0 41.0 20-21 37.979548648005164 40.0 38.0 41.0 33.0 41.0 22-23 37.88125089568695 40.0 38.0 41.0 33.0 41.0 24-25 37.771615673650786 40.0 38.0 41.0 32.0 41.0 26-27 37.58183252838079 40.0 37.5 41.0 32.0 41.0 28-29 37.36019033383216 40.0 37.0 41.0 31.5 41.0 30-31 37.13194360783557 40.0 37.0 41.0 31.0 41.0 32-33 36.86444823685988 40.0 36.0 41.0 30.5 41.0 34-35 36.61403643497469 39.0 36.0 41.0 30.0 41.0 36-37 36.33445621311269 39.0 35.0 41.0 30.0 41.0 38-39 36.029646880954 39.0 35.0 40.5 29.0 41.0 40-41 35.797014357826 39.0 35.0 40.0 28.0 41.0 42-43 35.594370147232375 39.0 35.0 40.0 27.0 41.0 44-45 35.307510238665245 39.0 35.0 40.0 26.0 41.0 46-47 34.906416344409685 38.0 34.5 40.0 24.5 41.0 48-49 34.49834708290108 38.0 34.0 40.0 23.5 41.0 50-51 33.79806263271154 37.5 33.0 39.5 22.0 40.5 52-53 33.93081328659869 38.0 33.5 40.0 22.5 40.5 54-55 34.02181850570585 38.0 34.0 40.0 21.5 41.0 56-57 33.701313027099275 38.0 33.0 40.0 19.5 41.0 58-59 33.35628385411165 37.5 33.0 40.0 18.0 41.0 60-61 32.91178047433868 37.0 32.5 40.0 14.0 41.0 62-63 32.34438656221221 36.0 31.5 39.0 8.5 41.0 64-65 31.798766906715528 36.0 31.0 39.0 7.0 40.5 66-67 31.238164799545977 35.0 30.5 39.0 2.0 40.0 68-69 30.60163498908618 35.0 29.5 38.0 2.0 40.0 70-71 30.019485009269467 35.0 29.0 37.5 2.0 39.5 72-73 29.34075756702022 34.0 28.0 37.0 2.0 39.0 74-75 28.609788809151738 34.0 26.5 36.0 2.0 39.0 76-77 27.90740438345621 34.0 26.0 35.5 2.0 38.5 78-79 27.150877359260818 33.0 25.0 35.0 2.0 37.0 80-81 26.461942368861443 32.5 23.5 35.0 2.0 37.0 82-83 25.699565902290047 32.0 20.5 35.0 2.0 36.0 84-85 25.007174916312152 31.5 18.0 35.0 2.0 36.0 86-87 24.290999909218023 31.0 11.0 34.0 2.0 35.0 88-89 23.444404604416114 30.5 4.5 34.0 2.0 35.0 90-91 22.578170417751245 30.0 2.0 34.0 2.0 35.0 92-93 21.656632308448604 29.0 2.0 34.0 2.0 35.0 94-95 20.67660434303254 29.0 2.0 34.0 2.0 35.0 96-97 19.460404213882335 26.5 2.0 33.5 2.0 35.0 98-99 17.96333607201808 24.0 2.0 33.0 2.0 35.0 100-101 14.450365954132835 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3054438290040302 1101 2 0.29390622653945186 1101 3 0.3386017041438265 1101 4 0.2412505297345504 1101 5 0.2616831638683905 1101 6 0.19837676485159506 1101 7 0.2873759278870551 1101 8 0.28228865615699306 1101 9 0.2417203209437062 1101 10-11 0.4325923607243993 1101 12-13 0.4275158617851531 1101 14-15 0.495666697491032 1101 16-17 0.7222556143777012 1101 18-19 0.5183349424894743 1101 20-21 0.6582554011564525 1101 22-23 0.6323593958163514 1101 24-25 0.6822117251661695 1101 26-27 0.7532702123468837 1101 28-29 0.8813450906725748 1101 30-31 0.7718131480121997 1101 32-33 0.6447112541493638 1101 34-35 0.7834145789311577 1101 36-37 0.8830114365425032 1101 38-39 0.8625596778524383 1101 40-41 1.0433791058444868 1101 42-43 1.2038915202833422 1101 44-45 1.258624578080088 1101 46-47 1.1742101411426447 1101 48-49 1.331540887114727 1101 50-51 1.3351704165787481 1101 52-53 1.2366924655799707 1101 54-55 1.3380266880191343 1101 56-57 1.4580303019635394 1101 58-59 1.375563637528984 1101 60-61 1.2310878013062663 1101 62-63 1.170649464133966 1101 64-65 1.3163112372446335 1101 66-67 1.590591763718887 1101 68-69 1.544275180082387 1101 70-71 1.9055422132465942 1101 72-73 1.9902163095352954 1101 74-75 2.1906754603044583 1101 76-77 2.2050074683370937 1101 78-79 2.373085063172958 1101 80-81 2.4029698489453537 1101 82-83 2.481659480145595 1101 84-85 2.5013291708723777 1101 86-87 2.8615290469795838 1101 88-89 3.2081931247375586 1101 90-91 3.4540908827151995 1101 92-93 3.851668358976262 1101 94-95 4.091169110631721 1101 96-97 4.364108106919815 1101 98-99 4.403845014896202 1101 100-101 4.211559786778031 1102 1 0.3496971787763279 1102 2 0.08449159020168295 1102 3 0.11337537717144386 1102 4 0.11767583642610902 1102 5 0.09940077635801003 1102 6 0.1869202357697688 1102 7 -0.0216483661774447 1102 8 0.11308543025929652 1102 9 0.2650701431599387 1102 10-11 0.20615890110386204 1102 12-13 0.27745850010624906 1102 14-15 0.3178432050757536 1102 16-17 0.30083778675058426 1102 18-19 0.36883539090056416 1102 20-21 0.31649688924901653 1102 22-23 0.2774478384571708 1102 24-25 0.21385923244972815 1102 26-27 0.37191841512520085 1102 28-29 0.330908825778927 1102 30-31 0.35608947573332017 1102 32-33 0.48142160554061064 1102 34-35 0.2347276580594908 1102 36-37 0.3278643593675383 1102 38-39 0.19254469476560843 1102 40-41 0.4398381556788493 1102 42-43 0.476566438046909 1102 44-45 0.4277194448961694 1102 46-47 0.2846546671163779 1102 48-49 0.527791246925112 1102 50-51 0.6724493931698632 1102 52-53 0.6151071285395417 1102 54-55 0.5406902000014497 1102 56-57 0.6484342629559237 1102 58-59 0.6948177776362314 1102 60-61 0.8108007766095469 1102 62-63 0.7819833249118915 1102 64-65 0.9379327633065486 1102 66-67 0.8928378956532406 1102 68-69 0.9909635518426398 1102 70-71 1.0531817739201905 1102 72-73 1.2463861217350676 1102 74-75 1.076723095039931 1102 76-77 1.3824149949880216 1102 78-79 1.900348499283112 1102 80-81 1.7907454054659517 1102 82-83 2.1932741594110325 1102 84-85 2.199032915063416 1102 86-87 2.46151182599694 1102 88-89 2.4530276618758116 1102 90-91 2.707635326993259 1102 92-93 2.762762332297882 1102 94-95 2.931839456099606 1102 96-97 3.036495134777873 1102 98-99 3.410631496341633 1102 100-101 3.402481833129931 1103 1 0.6364078742284711 1103 2 0.546374455120759 1103 3 0.5914829851828003 1103 4 0.5087649145265374 1103 5 0.4627215585352076 1103 6 0.5143282744142397 1103 7 0.516326102233613 1103 8 0.5485845361988737 1103 9 0.6859966626437526 1103 10-11 0.6733635200569807 1103 12-13 0.6895878001538875 1103 14-15 0.8993776856469964 1103 16-17 0.9789489853660527 1103 18-19 0.8439972673024059 1103 20-21 0.9099203387663195 1103 22-23 0.8767553306116938 1103 24-25 1.0282843647634863 1103 26-27 1.1466659358130613 1103 28-29 1.163796605038165 1103 30-31 1.1290814303863854 1103 32-33 1.093117399985701 1103 34-35 1.24118260674976 1103 36-37 1.3584184853307164 1103 38-39 1.365943105803602 1103 40-41 1.273894556888159 1103 42-43 1.483338078019436 1103 44-45 1.5159214379208237 1103 46-47 1.4518858565398958 1103 48-49 1.658278108904689 1103 50-51 1.5594480550713996 1103 52-53 1.7339968180001648 1103 54-55 1.825833955821217 1103 56-57 1.8900656419633393 1103 58-59 1.976791748150191 1103 60-61 1.9393803096873938 1103 62-63 2.0873778485225074 1103 64-65 2.397533440255117 1103 66-67 2.4585749150209644 1103 68-69 2.4804789878496933 1103 70-71 2.537110948057304 1103 72-73 2.4651101646595457 1103 74-75 2.513055939253192 1103 76-77 2.797376324181787 1103 78-79 3.095383411923713 1103 80-81 3.3189201577741123 1103 82-83 3.3288814622210374 1103 84-85 3.5635024511813 1103 86-87 3.579377235792588 1103 88-89 3.7913468282811635 1103 90-91 4.060757357264446 1103 92-93 4.333739000552395 1103 94-95 4.81192859883091 1103 96-97 4.782983643647864 1103 98-99 4.863027267088743 1103 100-101 4.660274521223725 1104 1 0.2608317511180438 1104 2 0.20761491686992528 1104 3 0.23616495842040308 1104 4 0.13286383161074156 1104 5 0.19533195554762273 1104 6 0.27807951853391444 1104 7 0.1800594820707957 1104 8 0.14332733306496692 1104 9 0.22140622258692844 1104 10-11 0.2792476744384089 1104 12-13 0.30705630372249004 1104 14-15 0.5603718755017795 1104 16-17 0.4920064998488769 1104 18-19 0.3324584295015782 1104 20-21 0.3927870031645071 1104 22-23 0.27217292948199656 1104 24-25 0.26598927233709446 1104 26-27 0.36592831108209367 1104 28-29 0.2680151277470699 1104 30-31 0.4115416823578357 1104 32-33 0.41584799809889006 1104 34-35 0.27658876397245535 1104 36-37 0.6240088998671354 1104 38-39 0.5963521553387068 1104 40-41 0.6672556365713689 1104 42-43 0.6215493529337195 1104 44-45 0.6423048564639515 1104 46-47 0.5522501603007584 1104 48-49 0.5689964657309687 1104 50-51 0.5243981201484331 1104 52-53 0.4195407854020985 1104 54-55 0.5957254090380673 1104 56-57 0.7877751857435911 1104 58-59 0.700544863766126 1104 60-61 0.8433704247844105 1104 62-63 1.1323539444111717 1104 64-65 1.0005956760126011 1104 66-67 0.9922105942465116 1104 68-69 1.0166903138047942 1104 70-71 1.3431105822648561 1104 72-73 1.4082762259255261 1104 74-75 1.669382021956313 1104 76-77 1.5302680836434774 1104 78-79 1.5456277697878278 1104 80-81 1.7949135394827884 1104 82-83 2.003972910333804 1104 84-85 2.2237871738170476 1104 86-87 2.4656081273904213 1104 88-89 2.5678163781863113 1104 90-91 2.6958991656402382 1104 92-93 2.9990697449825667 1104 94-95 3.208698779090028 1104 96-97 3.3167294859487804 1104 98-99 3.5005705454397855 1104 100-101 3.6026224408854954 1105 1 0.3871802310134349 1105 2 0.2721933590227401 1105 3 0.17133390846349528 1105 4 0.05240081636512173 1105 5 0.15186112431935328 1105 6 0.12998447856244866 1105 7 0.26558208303961806 1105 8 0.22341886624575125 1105 9 0.2894320183015111 1105 10-11 0.31433044064245763 1105 12-13 0.18943393753032467 1105 14-15 0.29118854167313657 1105 16-17 0.47855141946016033 1105 18-19 0.32567145217684157 1105 20-21 0.4434088641410554 1105 22-23 0.38847281952330803 1105 24-25 0.42960111815718705 1105 26-27 0.48163011499900676 1105 28-29 0.5449772960135775 1105 30-31 0.366109425827176 1105 32-33 0.5165567858786204 1105 34-35 0.41913386189513346 1105 36-37 0.5617262168750727 1105 38-39 0.5036190874912236 1105 40-41 0.6742432142111241 1105 42-43 0.5136040945307982 1105 44-45 0.765317427417024 1105 46-47 0.783248852683883 1105 48-49 0.7929444285233203 1105 50-51 0.9040713645241851 1105 52-53 0.8622359620789766 1105 54-55 0.9841842042474269 1105 56-57 1.1405271248799735 1105 58-59 1.0370853255926278 1105 60-61 1.1376510869140404 1105 62-63 1.0320872202886093 1105 64-65 1.1688064697954772 1105 66-67 1.433401268560747 1105 68-69 1.5762537480821877 1105 70-71 1.6456195721308262 1105 72-73 1.7590444508356278 1105 74-75 1.9134457385600747 1105 76-77 1.8325170360640541 1105 78-79 2.1398096176882113 1105 80-81 2.138378426998017 1105 82-83 2.328094726732509 1105 84-85 2.414944414745456 1105 86-87 2.5992970928871664 1105 88-89 2.753687429331965 1105 90-91 2.990257839003494 1105 92-93 3.1595908600231333 1105 94-95 3.620713252031276 1105 96-97 3.9953789257538297 1105 98-99 4.163053085782735 1105 100-101 3.8865944757156523 1106 1 0.06177022333669413 1106 2 -0.026684314354199046 1106 3 0.00577645663344839 1106 4 0.08591708026731482 1106 5 0.2494702323583553 1106 6 -0.21002116796493908 1106 7 -0.05905295461447224 1106 8 -0.002008588566170033 1106 9 -0.07043164026757154 1106 10-11 -0.14651660190951787 1106 12-13 0.06801393105902775 1106 14-15 0.22745399824880508 1106 16-17 0.10066487024972304 1106 18-19 0.20203264594155712 1106 20-21 0.054129018437173215 1106 22-23 0.08362743286306085 1106 24-25 0.17737692564231367 1106 26-27 -0.02465418448265666 1106 28-29 0.13072885515302346 1106 30-31 0.06770345954139145 1106 32-33 0.13537336256036525 1106 34-35 0.1590097651808975 1106 36-37 0.15938544577514335 1106 38-39 0.05084975130713332 1106 40-41 0.5220431304648727 1106 42-43 0.41188951697352394 1106 44-45 0.36488346958199713 1106 46-47 0.6434484479103446 1106 48-49 0.6017902846701091 1106 50-51 0.6282902086990703 1106 52-53 0.7307137471175835 1106 54-55 0.43756070073580133 1106 56-57 0.6966578895720446 1106 58-59 0.9443265211337746 1106 60-61 0.8565109642131006 1106 62-63 0.7130252734421063 1106 64-65 0.9177999287058896 1106 66-67 1.042959443653853 1106 68-69 0.7486047971308452 1106 70-71 0.9031200955448817 1106 72-73 1.3093689675499824 1106 74-75 1.47459889902931 1106 76-77 1.7035717629688065 1106 78-79 1.96161393696444 1106 80-81 2.1488666930065605 1106 82-83 2.2704148150467383 1106 84-85 2.518505375035776 1106 86-87 2.2698318951635414 1106 88-89 2.616159910441475 1106 90-91 2.7785907810980355 1106 92-93 3.333804787278094 1106 94-95 3.3233222331820222 1106 96-97 3.1715639034532153 1106 98-99 3.2516137922965456 1106 100-101 3.3349810915424296 1107 1 0.27275649050274 1107 2 0.14077709059693078 1107 3 0.07544546793030094 1107 4 0.04052260385063988 1107 5 0.11910422645110685 1107 6 0.12669969924372992 1107 7 0.1645927930789668 1107 8 0.18088322349801444 1107 9 0.22750843463879988 1107 10-11 0.13921617114854712 1107 12-13 0.044631573639946964 1107 14-15 0.1731750481559331 1107 16-17 0.29437699838224063 1107 18-19 0.28106419187499654 1107 20-21 0.3403109252000647 1107 22-23 0.25590827083998846 1107 24-25 0.2823881920471152 1107 26-27 0.31542909556362275 1107 28-29 0.3952450087415258 1107 30-31 0.45752563304402827 1107 32-33 0.43887051550942857 1107 34-35 0.4813111567341366 1107 36-37 0.6274999977071829 1107 38-39 0.6374777468005703 1107 40-41 0.40957624456005703 1107 42-43 0.7227372378102856 1107 44-45 0.5206826230791464 1107 46-47 0.6476566023514465 1107 48-49 0.6270110453046769 1107 50-51 0.5181397869701243 1107 52-53 0.5430184881425149 1107 54-55 0.5435790923803836 1107 56-57 0.6083406946023402 1107 58-59 0.7629998258443536 1107 60-61 0.9999650307762451 1107 62-63 1.0775048873393658 1107 64-65 1.1152634651390088 1107 66-67 1.261194272069556 1107 68-69 1.1935425274070255 1107 70-71 1.4512786473551174 1107 72-73 1.686912282308974 1107 74-75 1.6859596980305547 1107 76-77 1.8983001512639248 1107 78-79 1.893953847442198 1107 80-81 2.094567189337365 1107 82-83 2.192105689258849 1107 84-85 2.4801988374760207 1107 86-87 2.4023590715589016 1107 88-89 2.482797649852575 1107 90-91 2.8632608581358845 1107 92-93 2.981358911695743 1107 94-95 3.176549144664623 1107 96-97 3.240852292966327 1107 98-99 3.597634010925699 1107 100-101 3.476988034083762 1108 1 0.18826513899291442 1108 2 0.057108042824655314 1108 3 0.08092987633639126 1108 4 0.03678719974197975 1108 5 0.14753877464882947 1108 6 0.14604743361817185 1108 7 0.036884017310470085 1108 8 0.08238410658072581 1108 9 0.18071863692551915 1108 10-11 0.19655505324449507 1108 12-13 0.2930502478003021 1108 14-15 0.4156688451255661 1108 16-17 0.5164218890158381 1108 18-19 0.3813995487905615 1108 20-21 0.42916978212636536 1108 22-23 0.34405923150526263 1108 24-25 0.37683513915511213 1108 26-27 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-1.3670784489234933 2316 54-55 -1.2214605310144293 2316 56-57 -1.102546784713006 2316 58-59 -1.1923808809707737 2316 60-61 -1.2537380524412356 2316 62-63 -1.293629408560676 2316 64-65 -1.287199565649523 2316 66-67 -1.6727828692177305 2316 68-69 -1.6500815914490374 2316 70-71 -1.640641980046322 2316 72-73 -1.7060643698639915 2316 74-75 -1.8177562870212896 2316 76-77 -1.9999835838235427 2316 78-79 -2.539516977081302 2316 80-81 -2.758537274440574 2316 82-83 -2.459414223855962 2316 84-85 -2.391461545162862 2316 86-87 -2.139101647169557 2316 88-89 -2.512992629225014 2316 90-91 -2.3138583483948842 2316 92-93 -2.668228037039686 2316 94-95 -2.8406291663889753 2316 96-97 -3.167042859410113 2316 98-99 -3.364162052795244 2316 100-101 -3.1749308730730963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5950.0 3 1954.0 4 1998.0 5 2236.0 6 2637.0 7 3224.0 8 4029.0 9 5051.0 10 6300.0 11 7597.0 12 9137.0 13 11018.0 14 12989.0 15 15490.0 16 17938.0 17 21493.0 18 21501.0 19 18332.0 20 18901.0 21 20169.0 22 22068.0 23 24603.0 24 26956.0 25 30550.0 26 34484.0 27 38785.0 28 44213.0 29 51266.0 30 59746.0 31 71146.0 32 85851.0 33 106922.0 34 137151.0 35 183051.0 36 233987.0 37 255124.0 38 130113.0 39 7489.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79883731067297 18.397298835016496 14.797905874471764 23.005957979838765 2 15.669811843449821 19.348577701898016 40.690903129735545 24.290707324916617 3 19.468672664246004 23.518866781987153 29.50532538429372 27.50713516947313 4 12.898491442595866 16.539058759813393 36.519703338753004 34.04274645883773 5 13.887692045710393 36.74734596056856 34.191707991090155 15.173254002630895 6 32.575225112484816 35.60182947107852 17.168948362698814 14.653997053737855 7 28.913245639890235 32.46496717779286 20.17491848713959 18.44686869517732 8 26.265197188609356 35.10993953967905 19.283900761680094 19.340962510031503 9 26.101345994347326 17.063865615339445 20.023807946187645 36.81098044412558 10-11 25.134540431820373 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29.096541087086912 22.0861443546314 36-37 22.524905177535075 26.05355755609377 29.020186903075444 22.40135036329571 38-39 22.115295105326428 26.322527034783278 29.03211620887672 22.530061651013572 40-41 22.655481256471187 26.165841724104254 28.971702956715216 22.206974062709342 42-43 22.244428263445695 26.16624548478748 28.901186447937636 22.688139803829184 44-45 22.3034617996013 26.43511674001641 28.893575201904525 22.367846258477762 46-47 22.61581470788965 26.670464572392937 28.273437790018647 22.440282929698764 48-49 22.469974765639122 26.628090295436486 28.24763540303446 22.654299535889933 50-51 22.596465861665 26.945941771146426 28.085147328096284 22.37244503909229 52-53 22.756249284411286 26.75323874380739 28.010562018021556 22.479949953759764 54-55 22.533926419307672 26.832086602201542 28.14962715130002 22.484359827190758 56-57 22.49495630702885 27.051354016709713 28.015637304084006 22.43805237217743 58-59 22.552247341629105 26.714304109693078 27.9755152641297 22.757933284548116 60-61 22.334718696000262 26.936024340353796 28.13899424726154 22.590262716384398 62-63 22.361024859129692 26.88883273587478 28.291925244158538 22.458217160836984 64-65 22.21668622033294 26.864710234081514 28.195967994993044 22.722635550592507 66-67 22.159887397611485 26.8560979161135 28.058979212525585 22.925035473749425 68-69 22.347390817239006 26.604636263032212 28.243391246649512 22.804581673079273 70-71 22.338699891069872 26.70781170363581 27.937051805342 23.016436599952318 72-73 22.378271298685984 26.784240261518306 27.855930802380254 22.98155763741546 74-75 22.28830007931627 27.10383693168808 27.730858026161236 22.87700496283442 76-77 22.5305515553571 26.78041822598273 27.761602510589267 22.927427708070912 78-79 22.535278424314132 26.84143895770472 27.772622617935465 22.850660000045682 80-81 22.618397365156277 26.931055208084985 27.529834008842773 22.920713417915966 82-83 22.54127310343016 26.946821067370337 27.554461195488123 22.95744463371138 84-85 22.565934822071483 26.963340769981446 27.49113912051881 22.979585287428257 86-87 22.693212796796146 26.90814703622805 27.344942578515425 23.053697588460377 88-89 22.731871578071445 26.904193567823754 27.20546177848762 23.158473075617177 90-91 22.65594702510242 26.8404453336547 27.27160315432297 23.23200448691991 92-93 22.68578822908642 26.602563919533885 27.311042419625537 23.40060543175416 94-95 22.591101788694967 26.564868928510744 27.333692205105216 23.510337077689076 96-97 22.826092853926244 26.270871116296856 27.299652846490304 23.603383183286603 98-99 22.680693502575206 26.3155655446283 27.041483428701312 23.96225752409518 100-101 23.506381596665708 25.29257680873851 27.071138463579352 24.12990313101643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 796.0 1 563.5 2 436.0 3 572.0 4 464.5 5 397.0 6 765.0 7 1751.5 8 2893.5 9 3278.5 10 2635.5 11 1903.5 12 1788.5 13 1876.5 14 2005.5 15 2172.5 16 2303.5 17 2421.0 18 2569.5 19 2854.5 20 3207.5 21 3717.0 22 4488.0 23 5514.5 24 6804.5 25 8459.5 26 10609.5 27 13069.0 28 15126.5 29 17086.0 30 19502.5 31 22660.0 32 26764.5 33 29439.0 34 30981.0 35 33117.5 36 36250.0 37 40061.5 38 43002.5 39 44792.0 40 47044.5 41 50427.0 42 52901.5 43 55140.0 44 57758.5 45 60698.0 46 62350.0 47 64021.5 48 65230.0 49 64793.5 50 64507.0 51 63000.5 52 61786.0 53 62020.5 54 62384.5 55 59376.0 56 54614.0 57 50245.5 58 46362.0 59 41684.0 60 34357.0 61 28068.0 62 23282.0 63 17900.0 64 13379.0 65 10338.5 66 7708.5 67 5751.5 68 4405.0 69 3261.0 70 2421.0 71 1735.0 72 1143.5 73 819.0 74 589.0 75 401.0 76 300.0 77 200.5 78 113.0 79 74.0 80 44.5 81 30.5 82 23.5 83 12.0 84 7.5 85 6.0 86 6.5 87 10.0 88 7.0 89 1.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45134057571759156 2 0.041451392532697216 3 0.055097236630926735 4 0.059093927370993955 5 0.08421598345141651 6 0.08193216017137811 7 0.04596194351077308 8 0.04076624554868569 9 0.04413488488674235 10-11 0.049102200520825895 12-13 0.04487712745275483 14-15 0.038396778895645835 16-17 0.03939595158066264 18-19 0.049558965176833586 20-21 0.05743815549296612 22-23 0.04904510493882494 24-25 0.06648780524011833 26-27 0.058037659103976194 28-29 0.04601903909277404 30-31 0.048103027835809095 32-33 0.04653289933078268 34-35 0.051328928218863354 36-37 0.04619032583877692 38-39 0.0264923500484456 40-41 0.023865953276401425 42-43 0.02420852676840719 44-45 0.014273895500240087 46-47 0.027634261688464806 48-49 0.02646380225744512 50-51 0.015558546095261694 52-53 0.015330163767257854 54-55 0.015387259349258813 56-57 0.014388086664242008 58-59 0.013446009561226161 60-61 0.016158049706271778 62-63 0.016072406333270336 64-65 0.016300788661274178 66-67 0.008792719628147892 68-69 0.012047167802202633 70-71 0.018641707523313553 72-73 0.01658626657127898 74-75 0.0132461750242228 76-77 0.008393050554141171 78-79 0.013531652934227603 80-81 0.017214317973289546 82-83 0.014159704336238168 84-85 0.012104263384203595 86-87 0.00328299596505522 88-89 0.029546963685496978 90-91 0.033229628724558924 92-93 0.017242865764290027 94-95 0.03377203675356805 96-97 0.02823376529947489 98-99 0.024237074559407667 100-101 0.14799174854648922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1751449.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.59822816498897 #Duplication Level Percentage of deduplicated Percentage of total 1 81.89609766135682 52.0844670488919 2 11.011081826946608 14.00570588747028 3 3.03073279026384 5.782477065069401 4 1.294950623610643 3.2942626089113767 5 0.71719922254373 2.2806299897544418 6 0.4448891558580122 1.6976497225432323 7 0.29216040290336065 1.3006618775236125 8 0.22117167993135073 1.1252901571126364 9 0.14651988401439445 0.838656451282966 >10 0.8689043917518658 9.910358999574399 >50 0.04848098015251001 2.1263853589546815 >100 0.024391840150947714 2.8530807646562404 >500 0.0024296735243684187 1.0264901086855742 >1k 8.09891174789473E-4 0.8212607638067569 >5k 1.7997581661988288E-4 0.8526231957625829 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7994 0.456422082515677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6904 0.39418789813463023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4205 0.24008692231403828 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1874 0.10699712066979969 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 5.7095582000960346E-5 9 0.0 0.0 0.0 0.0 5.7095582000960346E-5 10-11 0.0 0.0 0.0 0.0 5.7095582000960346E-5 12-13 0.0 0.0 0.0 5.7095582000960346E-5 5.7095582000960346E-5 14-15 0.0 0.0 0.0 8.564337300144052E-5 5.7095582000960346E-5 16-17 0.0 0.0 0.0 1.1419116400192069E-4 5.7095582000960346E-5 18-19 0.0 0.0 0.0 1.1419116400192069E-4 1.1419116400192069E-4 20-21 0.0 0.0 0.0 1.7128674600288104E-4 1.7128674600288104E-4 22-23 0.0 0.0 0.0 4.5676465600768277E-4 1.7128674600288104E-4 24-25 0.0 0.0 0.0 7.136947750120044E-4 1.7128674600288104E-4 26-27 0.0 0.0 0.0 8.27885939013925E-4 1.7128674600288104E-4 28-29 0.0 0.0 0.0 0.0033115437560557 1.998345370033612E-4 30-31 0.0 0.0 0.0 0.010534134879177185 2.2838232800384138E-4 32-33 0.0 0.0 0.0 0.02586429864643504 2.8547791000480173E-4 34-35 0.0 0.0 0.0 0.04750352422479901 2.8547791000480173E-4 36-37 0.0 0.0 0.0 0.08458710473442276 2.8547791000480173E-4 38-39 0.0 0.0 0.0 0.14071206184136675 2.8547791000480173E-4 40-41 0.0 0.0 0.0 0.2011477353893833 2.8547791000480173E-4 42-43 0.0 0.0 0.0 0.25915684670235906 2.8547791000480173E-4 44-45 0.0 0.0 0.0 0.3286136222065273 3.1402570100528193E-4 46-47 0.0 0.0 0.0 0.4105457823779054 3.425734920057621E-4 48-49 0.0 0.0 0.0 0.4996719858814045 3.425734920057621E-4 50-51 0.0 0.0 0.0 0.5907108913819358 3.425734920057621E-4 52-53 0.0 0.0 0.0 0.6796372603484315 3.996690740067224E-4 54-55 0.0 0.0 0.0 0.7739020662320171 4.5676465600768277E-4 56-57 0.0 0.0 0.0 0.886922770802918 4.5676465600768277E-4 58-59 0.0 0.0 0.0 1.002084559698855 4.5676465600768277E-4 60-61 0.0 0.0 0.0 1.1240407228529063 4.5676465600768277E-4 62-63 0.0 0.0 0.0 1.247081702064976 5.138602380086432E-4 64-65 0.0 0.0 0.0 1.372834721422091 5.138602380086432E-4 66-67 0.0 0.0 0.0 1.5191136025085514 5.138602380086432E-4 68-69 0.0 0.0 0.0 1.6764975742941988 5.138602380086432E-4 70-71 0.0 0.0 0.0 1.8398194866079458 5.138602380086432E-4 72-73 0.0 0.0 0.0 2.0119341185498407 5.138602380086432E-4 74-75 0.0 0.0 0.0 2.1837347247907304 5.138602380086432E-4 76-77 0.0 0.0 0.0 2.3662122048658 6.280514020105639E-4 78-79 0.0 0.0 0.0 2.5764095900023354 6.851469840115241E-4 80-81 0.0 0.0 0.0 2.796313224079034 6.851469840115241E-4 82-83 0.0 0.0 0.0 3.0187576115547756 6.851469840115241E-4 84-85 0.0 0.0 0.0 3.231981062537362 6.851469840115241E-4 86-87 0.0 0.0 0.0 3.454139972103099 7.136947750120044E-4 88-89 0.0 0.0 0.0 3.6988516365592146 7.422425660124845E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3595 0.0 59.995407 1 GTATCAA 6470 0.0 42.997448 1 TGGTATC 1315 0.0 39.028843 2 GTGGTAT 1520 0.0 35.160324 1 ATCAACG 8210 0.0 33.45683 3 TATCAAC 8295 0.0 33.227623 2 TCAACGC 8310 0.0 33.22578 4 CAACGCA 8490 0.0 32.57732 5 AACGCAG 8700 0.0 31.844688 6 CGCAGAG 9780 0.0 27.929 8 ACGCAGA 9840 0.0 27.806976 7 GCAGAGT 10760 0.0 24.809227 9 GAGTACT 7155 0.0 23.17119 12-13 CAGAGTA 10125 0.0 23.151617 10-11 TACATGG 5605 0.0 22.298098 2 AGGACCG 715 0.0 21.933504 5 GTACATG 5850 0.0 21.860365 1 GTACTTT 7580 0.0 21.62226 14-15 AGAGTAC 9785 0.0 21.431818 10-11 ACATGGG 5760 0.0 21.203657 3 >>END_MODULE