FastQCFastQC Report
Thu 26 May 2016
SRR938130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751449
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78510.44825741428953964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69620.397499441890686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43250.24693839215415347No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19590.11185024513988133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36450.055.5225451
GTATCAA60900.040.4862331
ATCAACG77350.031.6881663
TCAACGC78250.031.3210184
TATCAAC78750.031.1283762
CAACGCA80100.030.7162175
AACGCAG83400.029.5577816
TGGTATC15400.028.3807852
GTGGTAT16900.027.2673111
GACCGTA1056.910369E-527.1414137
ACGCAGA95600.025.6367117
CGCAGAG95300.025.4183738
GAGTACT69000.022.88827512-13
GCAGAGT104600.022.7497469
GTACTTT72250.021.79295714-15
CAGAGTA100650.021.37740110-11
TACATGG55800.020.5182422
GTACATG58000.020.3952331
ACCGAGT6900.019.9627068
AGAGTAC97650.019.89397410-11
Produced by FastQC (version 0.11.5)