##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938130_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1751449 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46754373093364 33.0 31.0 34.0 31.0 34.0 2 32.70024762353914 34.0 31.0 34.0 31.0 34.0 3 32.66745534697271 34.0 31.0 34.0 31.0 34.0 4 36.197831053030946 37.0 35.0 37.0 35.0 37.0 5 36.15868289627617 37.0 35.0 37.0 35.0 37.0 6 36.21583957054987 37.0 37.0 37.0 35.0 37.0 7 36.17504763198929 37.0 37.0 37.0 35.0 37.0 8 36.17055763542073 37.0 37.0 37.0 35.0 37.0 9 37.87717255826461 39.0 38.0 39.0 35.0 39.0 10-11 37.8361827835124 39.0 38.0 39.0 35.0 39.0 12-13 37.7747119670627 39.0 38.0 39.0 35.0 39.0 14-15 39.039002277542764 40.0 38.0 41.0 36.0 41.0 16-17 38.96204456995322 40.0 38.0 41.0 36.0 41.0 18-19 38.9554811473243 40.0 38.0 41.0 35.5 41.0 20-21 38.961316315804794 40.0 38.5 41.0 35.0 41.0 22-23 38.98988351930316 40.0 38.5 41.0 35.0 41.0 24-25 38.94556307377491 40.0 38.0 41.0 35.0 41.0 26-27 38.77312984848545 40.0 38.0 41.0 35.0 41.0 28-29 38.68909200324988 40.0 38.0 41.0 34.5 41.0 30-31 38.47103284194972 40.0 38.0 41.0 34.0 41.0 32-33 38.2651758629569 40.0 38.0 41.0 34.0 41.0 34-35 38.06155503243315 40.0 38.0 41.0 33.5 41.0 36-37 37.98062661259334 40.0 37.5 41.0 33.0 41.0 38-39 37.94123579961506 40.0 38.0 41.0 33.0 41.0 40-41 37.81326119116228 40.0 37.0 41.0 33.0 41.0 42-43 37.62417261364733 40.0 37.0 41.0 33.0 41.0 44-45 37.36868872573509 40.0 37.0 41.0 32.5 41.0 46-47 37.01491679175357 40.0 36.5 41.0 31.0 41.0 48-49 36.64223280266796 40.0 36.0 41.0 30.5 41.0 50-51 36.33545395840815 39.0 35.5 41.0 30.0 41.0 52-53 35.86464036349331 39.0 35.0 40.0 28.5 41.0 54-55 35.538015094929975 38.5 35.0 40.0 28.0 41.0 56-57 35.258393478771005 38.0 35.0 40.0 27.0 41.0 58-59 34.870603711555404 38.0 34.0 40.0 26.5 41.0 60-61 34.44140451705988 37.5 34.0 40.0 26.0 41.0 62-63 33.99710553947046 37.0 33.0 40.0 25.0 41.0 64-65 33.54562736340024 36.0 33.0 39.0 23.5 41.0 66-67 33.02051615548041 36.0 32.0 39.0 22.5 40.0 68-69 32.47262866346665 35.0 31.5 38.5 21.0 40.0 70-71 31.87741864022304 35.0 31.0 37.5 19.0 40.0 72-73 31.387994454877074 35.0 31.0 37.0 18.0 39.0 74-75 30.820882023969865 34.0 30.0 36.5 14.5 39.0 76-77 29.62953702905423 33.5 28.5 35.0 13.0 37.5 78-79 30.324570398567133 34.0 30.0 36.0 12.0 37.5 80-81 30.126625439850088 34.0 30.0 35.0 7.5 37.0 82-83 29.651847413199015 34.0 29.5 35.0 4.5 37.0 84-85 29.12466306469672 34.0 29.0 35.0 2.0 36.0 86-87 28.5655571472535 34.0 29.0 35.0 2.0 36.0 88-89 27.927119487921146 33.0 27.0 35.0 2.0 35.0 90-91 27.377045520594663 33.0 26.0 35.0 2.0 35.0 92-93 26.71748934739179 32.5 25.0 35.0 2.0 35.0 94-95 25.958581151949044 32.0 23.5 34.0 2.0 35.0 96-97 25.121442873871864 32.0 19.5 34.0 2.0 35.0 98-99 24.078688560157904 31.5 6.0 34.0 2.0 35.0 100-101 20.627856706075942 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.01653232126380999 1101 2 0.04489758812853495 1101 3 0.057609321425474036 1101 4 0.0011994934361325704 1101 5 0.03451684548272027 1101 6 0.04697000276235741 1101 7 0.03626255972695702 1101 8 0.03888208140259053 1101 9 0.14359309910997808 1101 10-11 0.1634369504902864 1101 12-13 0.126943398556719 1101 14-15 0.2032909720933347 1101 16-17 0.17351600821676527 1101 18-19 0.19365747979093584 1101 20-21 0.2689149814479279 1101 22-23 0.18053598211292865 1101 24-25 0.2477259475433371 1101 26-27 0.2375298982060272 1101 28-29 0.156814698759689 1101 30-31 0.17546314928311801 1101 32-33 0.13168874998409308 1101 34-35 -0.04058585888004984 1101 36-37 0.05888070631879572 1101 38-39 0.1836250379988016 1101 40-41 0.17125275032204712 1101 42-43 0.23037123790307845 1101 44-45 0.20565520310021412 1101 46-47 0.061130601753987435 1101 48-49 0.24461432857938803 1101 50-51 0.40124833500880897 1101 52-53 0.34037033862595223 1101 54-55 0.3049912592309809 1101 56-57 0.40627456239004545 1101 58-59 0.3694324962715001 1101 60-61 0.5437343861959292 1101 62-63 0.6412765079662606 1101 64-65 0.5433021832196587 1101 66-67 0.6698102645448785 1101 68-69 0.6123850812957912 1101 70-71 0.6865647348704336 1101 72-73 0.7502889923864267 1101 74-75 0.8894030482907915 1101 76-77 0.9238829674640563 1101 78-79 0.8646089449410113 1101 80-81 1.02005246121983 1101 82-83 1.2237679285453673 1101 84-85 1.3107227341669443 1101 86-87 1.3517470497903297 1101 88-89 1.5850514542633078 1101 90-91 1.5996273950453421 1101 92-93 1.8031733555676652 1101 94-95 1.818742019666832 1101 96-97 1.9524639527971566 1101 98-99 2.2282657817666873 1101 100-101 2.6250884476092615 1102 1 0.016237401536884022 1102 2 0.04441673170494198 1102 3 0.10484472976242643 1102 4 0.025675750942234288 1102 5 -0.0247283264026521 1102 6 -0.07945239216885369 1102 7 0.0503675428304291 1102 8 0.08068372895979081 1102 9 0.021151496543637904 1102 10-11 0.09386813228230295 1102 12-13 0.037414671129106125 1102 14-15 0.14548608166703758 1102 16-17 0.09576888711581688 1102 18-19 0.20472903255785013 1102 20-21 0.1367961433567686 1102 22-23 0.09822634052567736 1102 24-25 0.023325936561839455 1102 26-27 0.0027344868075687145 1102 28-29 -0.17049399442562674 1102 30-31 -0.10320313136731585 1102 32-33 0.045727552934458515 1102 34-35 -0.20130504704798113 1102 36-37 0.10833009243651759 1102 38-39 0.1406950833057934 1102 40-41 -0.008723572906646382 1102 42-43 -0.18581124461388043 1102 44-45 -0.10368189721908294 1102 46-47 0.006773443565535331 1102 48-49 -0.016166672101128654 1102 50-51 0.02994419082508415 1102 52-53 -0.2008576921422005 1102 54-55 -0.055732379770631724 1102 56-57 0.043850455343751094 1102 58-59 0.05441579940571728 1102 60-61 0.1902968035227275 1102 62-63 0.07515001444118496 1102 64-65 0.04687761694716386 1102 66-67 0.0658265188230871 1102 68-69 0.06345128474298178 1102 70-71 0.4738502421240476 1102 72-73 0.41533110476040846 1102 74-75 0.5265238746982952 1102 76-77 0.5418593855329412 1102 78-79 0.6203214027828814 1102 80-81 0.6178262540455854 1102 82-83 0.5393979669306859 1102 84-85 0.7906869111953156 1102 86-87 0.9305916965605228 1102 88-89 1.0374267019709187 1102 90-91 1.0195691681576484 1102 92-93 1.2535690222027185 1102 94-95 1.205886556122902 1102 96-97 1.2803275443121187 1102 98-99 1.521635412272616 1102 100-101 1.7050782481287214 1103 1 0.411175607240196 1103 2 0.2977740662319519 1103 3 0.5363620951207579 1103 4 0.258543662192082 1103 5 0.12069879989326182 1103 6 0.24262766872779196 1103 7 0.26782793248278836 1103 8 0.2425481621111203 1103 9 0.23665141635347453 1103 10-11 0.33305718237601667 1103 12-13 0.3806589495014592 1103 14-15 0.4435157975066275 1103 16-17 0.46429365151234236 1103 18-19 0.5083182004233819 1103 20-21 0.4346626063962944 1103 22-23 0.56757766198767 1103 24-25 0.6613501946831875 1103 26-27 0.523458017472521 1103 28-29 0.5099762310882454 1103 30-31 0.45889110044686987 1103 32-33 0.5313974519427376 1103 34-35 0.5746067868650329 1103 36-37 0.5520607779785891 1103 38-39 0.569283712580706 1103 40-41 0.6014617740773716 1103 42-43 0.4555419822620266 1103 44-45 0.5255518439270972 1103 46-47 0.6904366736251148 1103 48-49 0.5913886770611825 1103 50-51 0.5678614489440363 1103 52-53 0.446096398393685 1103 54-55 0.6318869778942258 1103 56-57 0.7209900364878123 1103 58-59 0.9282204274550381 1103 60-61 0.9615602413586046 1103 62-63 1.1421645707502392 1103 64-65 1.0482556908035008 1103 66-67 1.025246947445467 1103 68-69 1.0715404370492294 1103 70-71 1.1003429160947995 1103 72-73 0.8748226273441055 1103 74-75 0.9821057081564994 1103 76-77 0.8123398322998447 1103 78-79 1.1431734435625707 1103 80-81 1.4042058957990449 1103 82-83 1.3539968844832337 1103 84-85 1.461945561559041 1103 86-87 1.5427530726715162 1103 88-89 1.766037592991296 1103 90-91 2.0026871051401507 1103 92-93 2.262610163344579 1103 94-95 2.2372105076066617 1103 96-97 2.445631536824518 1103 98-99 2.9135570317786943 1103 100-101 3.016017143228023 1104 1 0.14563963623137255 1104 2 0.1525229879948995 1104 3 0.24254271152357632 1104 4 0.11588205423651488 1104 5 0.04921359747392273 1104 6 0.10282859937183986 1104 7 0.0815723711615064 1104 8 0.058171753172175045 1104 9 0.0840551406310368 1104 10-11 0.11188375422020869 1104 12-13 0.08573942384068545 1104 14-15 0.20195241758437987 1104 16-17 0.15884142996122108 1104 18-19 0.12544515592430372 1104 20-21 0.15808991152674423 1104 22-23 0.12500112204399016 1104 24-25 0.08884226209108448 1104 26-27 0.128553378713832 1104 28-29 0.1454210942563492 1104 30-31 0.17360425111082378 1104 32-33 0.21269014545119802 1104 34-35 0.2400915043967018 1104 36-37 0.32649847842009194 1104 38-39 0.18001589295892018 1104 40-41 0.17718570285843072 1104 42-43 0.2093438843827755 1104 44-45 0.10753124896141486 1104 46-47 0.050165689473281816 1104 48-49 -0.028392400410261587 1104 50-51 0.14386078541354408 1104 52-53 0.13208357013749605 1104 54-55 0.13585369928797775 1104 56-57 0.2579418794862889 1104 58-59 0.21620754815803878 1104 60-61 0.29485415984643026 1104 62-63 0.2875706854389293 1104 64-65 0.3025161095082538 1104 66-67 0.364621314752128 1104 68-69 0.39484077817195384 1104 70-71 0.6008528988259947 1104 72-73 0.5122546792696241 1104 74-75 0.46660312634233847 1104 76-77 0.5083767262965928 1104 78-79 0.8333491515410998 1104 80-81 0.9034666547758512 1104 82-83 0.9173823361518423 1104 84-85 1.119003441834323 1104 86-87 1.1396929636143263 1104 88-89 1.2220940100967752 1104 90-91 1.1750180962675572 1104 92-93 1.1896600897607499 1104 94-95 1.4010753175221886 1104 96-97 2.011037353775169 1104 98-99 2.236916495169673 1104 100-101 2.621350694902592 1105 1 0.10087604741744371 1105 2 0.0725057512270908 1105 3 0.18437604980999822 1105 4 0.05931276540178487 1105 5 0.08762484297898965 1105 6 0.05572467354937771 1105 7 0.013204811947268524 1105 8 -0.03410439173555346 1105 9 0.1177485639125635 1105 10-11 0.04610171017728959 1105 12-13 0.07222862114224426 1105 14-15 -0.02775723203373559 1105 16-17 0.0808784890120009 1105 18-19 0.12000294367431508 1105 20-21 0.07176490929784762 1105 22-23 0.08404893921537138 1105 24-25 0.08832590202094792 1105 26-27 0.19454716753371315 1105 28-29 0.15695932719046368 1105 30-31 0.059830439110463374 1105 32-33 0.0390297097770258 1105 34-35 0.12580504714685503 1105 36-37 0.2205703676292856 1105 38-39 0.2006257179165729 1105 40-41 0.327015564072731 1105 42-43 0.3646568208742025 1105 44-45 0.1856873605843461 1105 46-47 0.14013493075831462 1105 48-49 0.3350247888078428 1105 50-51 0.4048417952503911 1105 52-53 0.28881185329598225 1105 54-55 0.35412170130884135 1105 56-57 0.3892385004854262 1105 58-59 0.45161573265089316 1105 60-61 0.39464910174663004 1105 62-63 0.35755419163525204 1105 64-65 0.3759098210598566 1105 66-67 0.3242374994827344 1105 68-69 0.5446037726145789 1105 70-71 0.657533632280181 1105 72-73 0.6583882939387031 1105 74-75 0.4721865017769744 1105 76-77 0.5901139250246388 1105 78-79 0.7871479271362141 1105 80-81 0.778485228879088 1105 82-83 0.8124386528328742 1105 84-85 0.9347446228501042 1105 86-87 1.0975854715640416 1105 88-89 0.9793081917960791 1105 90-91 1.0855466409494205 1105 92-93 1.2184276608624174 1105 94-95 1.2109507967340534 1105 96-97 1.3566865558316898 1105 98-99 1.579590125886785 1105 100-101 1.9591356216463254 1106 1 0.01594149003540224 1106 2 0.01801549307293726 1106 3 0.1210619823435124 1106 4 0.006334063595531347 1106 5 -0.014786351102941353 1106 6 -0.02151125612914484 1106 7 0.01670851391213546 1106 8 -0.01741382778070033 1106 9 -0.028903489456638454 1106 10-11 0.07867662183134172 1106 12-13 0.06173638987571195 1106 14-15 -0.053997881955382354 1106 16-17 0.019080418982376557 1106 18-19 -0.06695643305057075 1106 20-21 -0.0798365791013893 1106 22-23 -0.048040942080668 1106 24-25 -0.003726452073415487 1106 26-27 -0.007902410245620217 1106 28-29 -0.08054630347589153 1106 30-31 -0.05749710388878526 1106 32-33 -0.015330963745832094 1106 34-35 -0.01670727687499607 1106 36-37 0.01982632661899686 1106 38-39 0.043963132822135265 1106 40-41 0.10262922740012215 1106 42-43 0.16465396588844072 1106 44-45 0.05167543943270658 1106 46-47 -0.08624767539956935 1106 48-49 -0.002279089531697309 1106 50-51 -0.026380646206540348 1106 52-53 -0.03696613032274598 1106 54-55 0.028554828025804113 1106 56-57 0.19661657673257338 1106 58-59 0.20389813515312483 1106 60-61 0.10703244385640431 1106 62-63 0.3164325628975959 1106 64-65 0.2869779663976715 1106 66-67 0.2929644613337814 1106 68-69 0.07225573716200984 1106 70-71 0.29272418671842004 1106 72-73 0.37613314068941506 1106 74-75 0.3732328540568375 1106 76-77 0.550504487076168 1106 78-79 0.6478871146707093 1106 80-81 0.731850939865712 1106 82-83 0.6573342555203681 1106 84-85 0.7757983663708714 1106 86-87 0.9335909658255197 1106 88-89 1.160500021461285 1106 90-91 1.154609539457848 1106 92-93 0.8744381989320686 1106 94-95 1.3085316721361302 1106 96-97 1.3871763762213654 1106 98-99 1.907861780953752 1106 100-101 2.1432350956945037 1107 1 0.0360413651914584 1107 2 0.0586872143968904 1107 3 0.07201658177714876 1107 4 0.04254946507538904 1107 5 -0.012019991978675648 1107 6 0.06201695555746056 1107 7 -0.006577926817442403 1107 8 -0.016550577575159764 1107 9 -0.007093861247994937 1107 10-11 -0.02593879791835718 1107 12-13 0.030702773359770674 1107 14-15 0.0303928186931941 1107 16-17 0.035287294534278146 1107 18-19 -0.001393569556775276 1107 20-21 -0.04063786408053005 1107 22-23 -0.02982514475414888 1107 24-25 0.09325597842504152 1107 26-27 0.07484476554074604 1107 28-29 -0.05323288427427997 1107 30-31 -0.046034298249502115 1107 32-33 9.756347134128873E-4 1107 34-35 -0.12310933527760426 1107 36-37 0.00972995318335279 1107 38-39 0.15256214016051928 1107 40-41 0.18795092893174115 1107 42-43 0.1434959621735743 1107 44-45 -0.029190703498379378 1107 46-47 0.08809537140168544 1107 48-49 0.04793253618006332 1107 50-51 0.025129661031364492 1107 52-53 -0.061261980022813134 1107 54-55 -0.01002575721825849 1107 56-57 -0.02660972575516496 1107 58-59 -0.03735517625639062 1107 60-61 0.07415128920216318 1107 62-63 0.16498924509363633 1107 64-65 0.22402160087895595 1107 66-67 0.09115803034812586 1107 68-69 0.31788391535390303 1107 70-71 0.3851939653487868 1107 72-73 0.13892736132825334 1107 74-75 0.03306126975672541 1107 76-77 0.22887603483658836 1107 78-79 0.41220458368809787 1107 80-81 0.47802340720836156 1107 82-83 0.5392166586140625 1107 84-85 0.6201164443831146 1107 86-87 0.5877778054467235 1107 88-89 0.6944294085079648 1107 90-91 0.6937047253535304 1107 92-93 0.8600240519816076 1107 94-95 1.1498458830310447 1107 96-97 1.1096597049872372 1107 98-99 1.2743472716513509 1107 100-101 1.722224011988164 1108 1 0.053690148539445204 1108 2 0.03169134021651132 1108 3 0.12153928808600512 1108 4 0.045741433670535514 1108 5 -0.0060836740402976375 1108 6 -0.02140897632718719 1108 7 0.024658174461777094 1108 8 0.044637401059240744 1108 9 0.03671419160825451 1108 10-11 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-3.8740397194790894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 10.0 5 34.0 6 59.0 7 158.0 8 387.0 9 755.0 10 1341.0 11 2140.0 12 3292.0 13 4845.0 14 6900.0 15 9268.0 16 12034.0 17 16218.0 18 16756.0 19 12837.0 20 11652.0 21 12031.0 22 12743.0 23 13636.0 24 15656.0 25 18106.0 26 21096.0 27 25184.0 28 30606.0 29 36941.0 30 44757.0 31 54465.0 32 68530.0 33 88425.0 34 121738.0 35 179092.0 36 270880.0 37 376131.0 38 245327.0 39 17415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.984408909423 18.843768788014952 15.119195591764306 23.052626710797743 2 15.873351236868569 19.674435841636512 40.11227492338273 24.33993799811219 3 19.76740268809315 23.449013419910205 29.59568973245257 27.18789415954408 4 13.184740177989768 16.838800330469226 36.29531890451849 33.68114058702252 5 13.922013974770502 36.792425898202744 34.26576500963206 15.019795117394697 6 32.726843990622605 35.73864109998253 16.943428458542257 14.591086450852611 7 28.903724858674163 32.47745152727827 20.051568729663266 18.5672548843843 8 26.369738850219278 35.230640721643304 19.117335551689546 19.282284876447875 9 25.914828236505887 16.962869030157314 20.072751190585624 37.049551542751175 10-11 25.141068909228874 26.454666964324968 26.726641769186543 21.67762235725962 12-13 26.025793500124756 23.663121221343015 27.3780167164445 22.933068562087737 14-15 22.767805399985956 25.220231933673205 25.837692105222594 26.174270561118252 16-17 22.230821451266923 28.398114932264658 26.70446013557917 22.666603480889254 18-19 22.103955068060788 26.764924356918186 29.151633875722332 21.979486699298693 20-21 22.566943142506577 25.84788366660976 29.740118039406227 21.845055151477432 22-23 22.483212471502167 25.742171196534986 29.52212710732656 22.25248922463629 24-25 22.32758733898093 25.938336997972506 29.547088281928467 22.186987381118097 26-27 21.890068521215824 26.29339255829386 29.517090613789847 22.29944830670047 28-29 22.031351255677457 26.468458901078822 29.372432477183125 22.127757366060596 30-31 22.148835262738544 26.165086972396818 29.320478815653235 22.36559894921141 32-33 21.933395931607055 26.19186761064635 29.315605212919298 22.55913124482729 34-35 22.48730493426547 26.34886054914636 29.109211114267726 22.054623402320445 36-37 22.509745522940737 26.038689740349692 29.075861194746583 22.37570354196299 38-39 22.10166746474944 26.30686411148831 29.09555663489469 22.49591178886756 40-41 22.641644663145534 26.140754195136562 28.993221478253993 22.224379663463914 42-43 22.274744028765518 26.139289353783813 28.96356027227076 22.62240634517991 44-45 22.297081432945763 26.41091197190638 29.016794786692646 22.275211808455207 46-47 22.622930882585525 26.63857883729176 28.329244249350953 22.409246030771758 48-49 22.48705426460601 26.60202672592439 28.31586823569446 22.595050773775146 50-51 22.566486377850566 26.907691859711587 28.1510338011555 22.374787961282344 52-53 22.792841812693375 26.752391876668973 28.041895596160664 22.412870714476984 54-55 22.578219519951766 26.8370360769854 28.119659778845975 22.465084624216864 56-57 22.522979544365835 27.01260499163835 28.079978349355304 22.384437114640505 58-59 22.61830061851644 26.700549088212107 27.92701928517473 22.754131008096724 60-61 22.33402171573366 26.979632293032797 28.115434705777904 22.57091128545564 62-63 22.420236044555107 26.914600425133706 28.267651527392463 22.397512002918727 64-65 22.204957152620487 26.930644283675974 28.192856314971205 22.671542248732337 66-67 22.18457714808874 26.93870466497264 28.045561197558705 22.831156989379924 68-69 22.358591964646386 26.708536795993027 28.209352549819506 22.72351868954108 70-71 22.375444560475355 26.82584534291321 27.87997252560594 22.918737571005494 72-73 22.37404571871633 26.946945072337247 27.73660551920153 22.942403689744893 74-75 22.263651410917472 27.285636064766944 27.590612116025078 22.860100408290506 76-77 22.446214534365545 26.90198230151149 27.799924519640594 22.85187864448237 78-79 22.473249292443 26.97292356214768 27.836437144330205 22.717390001079107 80-81 22.471492159630202 27.078337467055828 27.642584878340664 22.8075854949733 82-83 22.41900705907475 27.15332897309547 27.69585599453248 22.7318079732973 84-85 22.49506886178567 27.170635796366334 27.575690009623894 22.758605332224107 86-87 22.564943777627295 27.149433779557143 27.453526759154478 22.832095683661084 88-89 22.542582635220295 27.28682989355947 27.27247034238822 22.89811712883201 90-91 22.49612161429918 27.18598493867383 27.37740618360566 22.94048726342133 92-93 22.49056485272035 26.954117492563217 27.473750021411075 23.081567633305355 94-95 22.343610740148005 26.98411656190567 27.44584906080515 23.226423637141174 96-97 22.512890067090296 26.755956722604274 27.462573057511637 23.268580152793792 98-99 22.383412796223006 26.827225381991397 27.22409733897096 23.565264482814634 100-101 22.66449697384949 26.53340184994861 27.335160443074113 23.466940733127785 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 779.0 1 542.5 2 447.5 3 623.5 4 492.0 5 392.5 6 818.5 7 1860.5 8 2983.5 9 3488.5 10 2821.0 11 1896.0 12 1785.5 13 1879.0 14 1999.5 15 2118.0 16 2188.5 17 2372.5 18 2546.0 19 2771.0 20 3103.0 21 3603.0 22 4429.5 23 5399.5 24 6580.5 25 8333.5 26 10490.0 27 12790.0 28 14983.0 29 17144.5 30 19467.5 31 22596.5 32 26817.5 33 29375.0 34 30860.0 35 33158.0 36 36453.0 37 39940.5 38 42882.5 39 45392.0 40 47904.0 41 51149.0 42 53336.5 43 55394.0 44 58396.0 45 61224.0 46 62933.5 47 65472.5 48 66509.0 49 65947.5 50 65626.0 51 63385.0 52 62224.0 53 62896.0 54 63430.5 55 60049.0 56 54514.0 57 49739.0 58 46159.5 59 41457.5 60 33448.0 61 27083.5 62 22273.0 63 16755.5 64 12171.5 65 9173.0 66 6754.5 67 5072.5 68 3836.0 69 2761.0 70 2022.0 71 1337.5 72 867.5 73 660.5 74 462.0 75 303.0 76 215.5 77 128.5 78 64.5 79 34.5 80 24.5 81 17.0 82 9.5 83 6.0 84 4.5 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.012104263384203595 3 0.005481175872092193 4 0.0 5 3.996690740067224E-4 6 1.7128674600288104E-4 7 0.0 8 1.141911640019207E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014844851320249689 26-27 0.022010346861370215 28-29 2.283823280038414E-4 30-31 1.4273895500240086E-4 32-33 0.003968142949066744 34-35 0.015786928423265536 36-37 0.007536616824126766 38-39 0.02155358220536253 40-41 0.0230380673373875 42-43 0.018527516359311633 44-45 0.009220936493155096 46-47 0.0021696321160364932 48-49 1.998345370033612E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.4273895500240086E-4 68-69 8.564337300144052E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.709558200096035E-5 82-83 8.849815210148855E-4 84-85 0.004482003187075387 86-87 6.280514020105639E-4 88-89 8.564337300144052E-5 90-91 0.0043392642320729865 92-93 0.0010848160580182466 94-95 1.141911640019207E-4 96-97 1.998345370033612E-4 98-99 1.4273895500240086E-4 100-101 0.002797683518047057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1751449.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.84374995067755 #Duplication Level Percentage of deduplicated Percentage of total 1 80.77796563261938 49.95612308108138 2 11.54447750074427 14.279075597345031 3 3.305264714805658 6.132298936297159 4 1.4187340390704022 3.50959332635139 5 0.7565950551302353 2.3395337701696683 6 0.45266687689810975 1.67967702875045 7 0.31225850566042374 1.3517865860824836 8 0.22872978699466648 1.1316406202535914 9 0.1720756561322236 0.9577623465396009 >10 0.9481745295125101 10.580540518843504 >50 0.05265664734121032 2.246676365120091 >100 0.026613141555651126 3.0096167397628566 >500 0.0025868677802276165 1.022510995704746 >1k 0.0010162694850894209 0.9568042654264459 >5k 1.8477627001625832E-4 0.8463598222717034 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7851 0.44825741428953964 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6962 0.397499441890686 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4325 0.24693839215415347 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1959 0.11185024513988133 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.7095582000960346E-5 5.7095582000960346E-5 14-15 0.0 0.0 0.0 5.7095582000960346E-5 5.7095582000960346E-5 16-17 0.0 0.0 0.0 5.7095582000960346E-5 5.7095582000960346E-5 18-19 0.0 0.0 0.0 5.7095582000960346E-5 5.7095582000960346E-5 20-21 0.0 0.0 0.0 1.1419116400192069E-4 5.7095582000960346E-5 22-23 0.0 5.7095582000960346E-5 0.0 3.996690740067224E-4 5.7095582000960346E-5 24-25 0.0 5.7095582000960346E-5 0.0 6.56599193011044E-4 5.7095582000960346E-5 26-27 0.0 5.7095582000960346E-5 0.0 7.707903570129647E-4 5.7095582000960346E-5 28-29 0.0 5.7095582000960346E-5 0.0 0.0032544481740547394 5.7095582000960346E-5 30-31 0.0 5.7095582000960346E-5 0.0 0.010534134879177185 5.7095582000960346E-5 32-33 0.0 5.7095582000960346E-5 0.0 0.025892846437435516 5.7095582000960346E-5 34-35 0.0 5.7095582000960346E-5 0.0 0.04784609771680477 5.7095582000960346E-5 36-37 0.0 5.7095582000960346E-5 0.0 0.08507241718143091 5.7095582000960346E-5 38-39 0.0 5.7095582000960346E-5 0.0 0.14171123452638357 5.7095582000960346E-5 40-41 0.0 5.7095582000960346E-5 0.0 0.2024323859844049 5.7095582000960346E-5 42-43 0.0 5.7095582000960346E-5 0.0 0.26044149729738064 8.564337300144052E-5 44-45 0.0 5.7095582000960346E-5 0.0 0.3301552029205532 1.4273895500240086E-4 46-47 0.0 5.7095582000960346E-5 0.0 0.4120588153009308 1.7128674600288104E-4 48-49 0.0 5.7095582000960346E-5 0.0 0.5008424453124242 1.7128674600288104E-4 50-51 0.0 5.7095582000960346E-5 0.0 0.5919384463949564 2.8547791000480173E-4 52-53 0.0 5.7095582000960346E-5 0.0 0.6807791719884507 2.8547791000480173E-4 54-55 0.0 5.7095582000960346E-5 0.0 0.7751296212450377 2.8547791000480173E-4 56-57 0.0 5.7095582000960346E-5 0.0 0.8884643515169439 2.8547791000480173E-4 58-59 0.0 5.7095582000960346E-5 0.0 1.003683235994882 2.8547791000480173E-4 60-61 0.0 5.7095582000960346E-5 0.0 1.1264101895059462 2.8547791000480173E-4 62-63 0.0 5.7095582000960346E-5 0.0 1.2501363157020273 3.425734920057621E-4 64-65 0.0 5.7095582000960346E-5 0.0 1.3769170555351598 3.425734920057621E-4 66-67 0.0 5.7095582000960346E-5 0.0 1.5239952747696335 3.425734920057621E-4 68-69 0.0 5.7095582000960346E-5 0.0 1.6820643935392923 3.425734920057621E-4 70-71 0.0 5.7095582000960346E-5 0.0 1.846728052030062 3.711212830062422E-4 72-73 0.0 5.7095582000960346E-5 0.0 2.0199560478209757 3.996690740067224E-4 74-75 0.0 5.7095582000960346E-5 0.0 2.193326782566892 3.996690740067224E-4 76-77 0.0 5.7095582000960346E-5 0.0 2.3786304939510083 3.996690740067224E-4 78-79 0.0 5.7095582000960346E-5 0.0 2.5924819963356054 3.996690740067224E-4 80-81 0.0 5.7095582000960346E-5 0.0 2.8165821556893746 4.282168650072026E-4 82-83 0.0 5.7095582000960346E-5 0.0 3.0452214138122207 5.138602380086432E-4 84-85 0.0 5.7095582000960346E-5 0.0 3.2634121804288907 5.138602380086432E-4 86-87 2.8547791000480173E-5 5.7095582000960346E-5 0.0 3.4934502803107597 5.138602380086432E-4 88-89 5.7095582000960346E-5 5.7095582000960346E-5 0.0 3.746298065202013 5.138602380086432E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3645 0.0 55.522545 1 GTATCAA 6090 0.0 40.486233 1 ATCAACG 7735 0.0 31.688166 3 TCAACGC 7825 0.0 31.321018 4 TATCAAC 7875 0.0 31.128376 2 CAACGCA 8010 0.0 30.716217 5 AACGCAG 8340 0.0 29.557781 6 TGGTATC 1540 0.0 28.380785 2 GTGGTAT 1690 0.0 27.267311 1 GACCGTA 105 6.910369E-5 27.141413 7 ACGCAGA 9560 0.0 25.636711 7 CGCAGAG 9530 0.0 25.418373 8 GAGTACT 6900 0.0 22.888275 12-13 GCAGAGT 10460 0.0 22.749746 9 GTACTTT 7225 0.0 21.792957 14-15 CAGAGTA 10065 0.0 21.377401 10-11 TACATGG 5580 0.0 20.518242 2 GTACATG 5800 0.0 20.395233 1 ACCGAGT 690 0.0 19.962706 8 AGAGTAC 9765 0.0 19.893974 10-11 >>END_MODULE