FastQCFastQC Report
Thu 26 May 2016
SRR938129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771973
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84710.47805468819220154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72000.4063267329694076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42810.24159510331139356No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.11117550888190735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36200.060.0139851
GTATCAA65650.043.56541
ATCAACG80300.035.035963
TCAACGC81350.034.7589954
TATCAAC81050.034.711752
CAACGCA82950.034.031255
AACGCAG85200.033.1883166
ACGCAGA95450.029.3903567
CGCAGAG95450.029.1412878
GGACCGA5650.026.9159286
TGGTATC15050.026.2089392
GCAGAGT105450.025.698519
GTGGTAT15950.024.8466011
GAGTACT67550.024.84232312-13
CAGAGTA100350.023.47468610-11
GTACTTT72350.023.04778514-15
GTAAGGT9200.022.2120424
AGGACCG6500.021.9338955
GTACATG58050.021.6323221
TACATGG56650.021.5596182
Produced by FastQC (version 0.11.5)