##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938129_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1771973 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.776168147031587 33.0 31.0 34.0 30.0 34.0 2 31.97039796881781 34.0 31.0 34.0 30.0 34.0 3 32.032345300972416 34.0 31.0 34.0 30.0 34.0 4 35.55818514164719 37.0 35.0 37.0 33.0 37.0 5 35.57194381629969 37.0 35.0 37.0 33.0 37.0 6 35.59496899783462 37.0 35.0 37.0 33.0 37.0 7 35.53395903887926 37.0 35.0 37.0 33.0 37.0 8 35.512248775799634 37.0 35.0 37.0 33.0 37.0 9 37.161342751836514 39.0 37.0 39.0 34.0 39.0 10-11 37.07255866765464 39.0 37.0 39.0 33.0 39.0 12-13 36.96731383604603 39.0 37.0 39.0 33.0 39.0 14-15 38.19450381015963 40.0 38.0 41.0 33.0 41.0 16-17 38.104623490312775 40.0 38.0 41.0 33.0 41.0 18-19 38.043047495644686 40.0 38.0 41.0 33.0 41.0 20-21 37.934425919582296 40.0 38.0 41.0 33.0 41.0 22-23 37.77609901505271 40.0 38.0 41.0 32.0 41.0 24-25 37.72612195558284 40.0 38.0 41.0 32.0 41.0 26-27 37.7319217053533 40.0 38.0 41.0 32.0 41.0 28-29 37.638527505780274 40.0 38.0 41.0 32.0 41.0 30-31 37.443170973824095 40.0 37.0 41.0 32.0 41.0 32-33 37.20639676789658 40.0 37.0 41.0 31.0 41.0 34-35 36.98998235300425 40.0 36.0 41.0 30.5 41.0 36-37 36.76241426929191 40.0 36.0 41.0 30.0 41.0 38-39 36.51159752434151 39.0 35.5 41.0 30.0 41.0 40-41 36.27660466609819 39.0 35.0 41.0 30.0 41.0 42-43 35.9875568081455 39.0 35.0 41.0 28.5 41.0 44-45 35.745188555356094 39.0 35.0 40.5 27.5 41.0 46-47 35.58245074840305 39.0 35.0 41.0 26.5 41.0 48-49 35.310670083573505 39.0 35.0 41.0 25.5 41.0 50-51 34.66656658989725 38.5 34.0 40.0 24.0 40.5 52-53 34.74373650162841 38.5 34.5 40.0 24.0 41.0 54-55 34.87469081075163 39.0 35.0 40.0 24.0 41.0 56-57 34.652453508038775 38.5 34.0 40.0 23.5 41.0 58-59 34.37385389055025 38.0 34.0 40.0 23.0 41.0 60-61 33.992532335424976 38.0 34.0 40.0 22.0 41.0 62-63 33.561381860784564 37.0 33.5 40.0 20.5 41.0 64-65 33.11356380712347 36.5 33.0 39.5 18.5 41.0 66-67 32.67159516538909 36.0 32.0 39.0 17.0 41.0 68-69 32.17274952835061 35.5 32.0 39.0 12.0 40.5 70-71 31.68569413868044 35.0 31.0 38.5 8.0 40.0 72-73 31.182505038169317 35.0 31.0 37.5 7.0 40.0 74-75 30.640204167896464 35.0 30.0 37.0 2.0 39.0 76-77 30.08908205711938 34.5 29.5 36.0 2.0 39.0 78-79 29.509022146499973 34.0 29.0 36.0 2.0 38.5 80-81 28.967576819737097 34.0 29.0 35.0 2.0 37.0 82-83 28.421285200169528 34.0 27.5 35.0 2.0 37.0 84-85 27.90969896268171 34.0 26.5 35.0 2.0 36.0 86-87 27.3918208686024 33.0 26.0 35.0 2.0 36.0 88-89 26.824452460618758 33.0 25.0 35.0 2.0 35.5 90-91 26.19353342291333 32.5 24.0 35.0 2.0 35.0 92-93 25.555326745949287 32.0 21.5 35.0 2.0 35.0 94-95 24.922016588288876 32.0 18.5 34.0 2.0 35.0 96-97 24.056269198232705 31.5 4.5 34.0 2.0 35.0 98-99 22.948882686135736 31.0 2.0 34.0 2.0 35.0 100-101 20.10723950082761 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38399262310526083 1101 2 0.3498511463221874 1101 3 0.37998312418925906 1101 4 0.21487626655671477 1101 5 0.2677407102992859 1101 6 0.28224225450806273 1101 7 0.24863895619274956 1101 8 0.35770932177779 1101 9 0.20977119935630384 1101 10-11 0.2792617761518912 1101 12-13 0.0841886005028627 1101 14-15 0.33589820746676935 1101 16-17 0.4847400337244707 1101 18-19 0.33505221176924493 1101 20-21 0.3615010470700071 1101 22-23 0.4389926113212965 1101 24-25 0.41837209346717685 1101 26-27 0.4638538704422288 1101 28-29 0.29442203470610195 1101 30-31 0.4595339165259631 1101 32-33 0.4344698698727072 1101 34-35 0.5384937543213155 1101 36-37 0.45331873844242665 1101 38-39 0.3857323547230962 1101 40-41 0.42723633350253465 1101 42-43 0.61462108428384 1101 44-45 0.7167954134991774 1101 46-47 0.7851698677766379 1101 48-49 0.999283579310962 1101 50-51 0.9317568138959373 1101 52-53 0.8898622550276016 1101 54-55 1.0366329968954346 1101 56-57 0.8600474628694954 1101 58-59 0.6114004901352317 1101 60-61 0.7529224455111603 1101 62-63 0.8694306700833678 1101 64-65 0.8589232756214926 1101 66-67 0.7263087364117737 1101 68-69 0.7051800456321757 1101 70-71 0.8188856578129737 1101 72-73 0.8032796900873187 1101 74-75 0.970986445146508 1101 76-77 0.8867401198621074 1101 78-79 0.8281714630851233 1101 80-81 0.8071912311529346 1101 82-83 0.7818113640316326 1101 84-85 0.9227634934159674 1101 86-87 0.9962340919889883 1101 88-89 0.8671364616265436 1101 90-91 0.9306101894233869 1101 92-93 1.0914517886787536 1101 94-95 1.2794722947086399 1101 96-97 1.4121808729463794 1101 98-99 1.1509067986950647 1101 100-101 1.1486322285248356 1102 1 0.5818717483387665 1102 2 0.5221685293405827 1102 3 0.32928870424260026 1102 4 0.331923088731223 1102 5 0.3397017154751367 1102 6 0.3781660598752268 1102 7 0.34888088949332285 1102 8 0.440012585867386 1102 9 0.6495417852221195 1102 10-11 0.6575899998167216 1102 12-13 0.4128704537004566 1102 14-15 0.5261259562072667 1102 16-17 0.5365649524442375 1102 18-19 0.6075719442233449 1102 20-21 0.5789302945007435 1102 22-23 0.5402555211988869 1102 24-25 0.5743671826067569 1102 26-27 0.627109214674185 1102 28-29 0.536507312707883 1102 30-31 0.5486923676605997 1102 32-33 0.69003581248964 1102 34-35 0.68364471036395 1102 36-37 0.779626585236052 1102 38-39 0.7920172469770534 1102 40-41 0.9532492907577037 1102 42-43 1.0439271729795934 1102 44-45 0.7917742269020565 1102 46-47 0.8171173282973783 1102 48-49 0.8357025398591773 1102 50-51 0.9571136634633035 1102 52-53 0.9992794410701293 1102 54-55 1.0226662506235016 1102 56-57 0.8538063384744348 1102 58-59 0.8913816785700206 1102 60-61 0.7844443282100215 1102 62-63 0.9598092477058842 1102 64-65 0.915591167405367 1102 66-67 0.9636384129890416 1102 68-69 0.8825046850088043 1102 70-71 1.0963398369256545 1102 72-73 1.2566962493511333 1102 74-75 1.0242669425659692 1102 76-77 0.9570250662618669 1102 78-79 0.9690543350442447 1102 80-81 1.0534137408766178 1102 82-83 1.1623125983098603 1102 84-85 1.1479911724232252 1102 86-87 1.0533917571890683 1102 88-89 1.1933637050061954 1102 90-91 1.4434152620649066 1102 92-93 1.4332336450758767 1102 94-95 1.4575367216532342 1102 96-97 1.546650338404227 1102 98-99 1.4945177167864117 1102 100-101 1.3954244997854737 1103 1 0.43736655977499694 1103 2 0.4929771143149466 1103 3 0.4547471490095134 1103 4 0.12026737428332979 1103 5 0.19280979530109477 1103 6 0.32731489137683667 1103 7 0.3889900281152805 1103 8 0.38937881421362164 1103 9 0.49680486320618655 1103 10-11 0.4333503674838255 1103 12-13 0.27523662721480235 1103 14-15 0.3978015800358108 1103 16-17 0.5118755441884062 1103 18-19 0.2891266907725978 1103 20-21 0.4961899435612267 1103 22-23 0.4718010003870958 1103 24-25 0.5783805291512252 1103 26-27 0.3414022656207294 1103 28-29 0.44996348233766525 1103 30-31 0.5366853430009186 1103 32-33 0.5443125451579434 1103 34-35 0.560751393907509 1103 36-37 0.6173908112144701 1103 38-39 0.5724033792979668 1103 40-41 0.7005596322831238 1103 42-43 0.6763129701309651 1103 44-45 0.7248959297487616 1103 46-47 0.7274113971090799 1103 48-49 0.7037492195567907 1103 50-51 0.644065103263685 1103 52-53 0.6363483446718092 1103 54-55 0.6197839468811068 1103 56-57 0.6445751973610996 1103 58-59 0.8058564856351467 1103 60-61 0.7432049576800708 1103 62-63 0.6917405966397965 1103 64-65 0.5553230858185998 1103 66-67 0.8448663692192895 1103 68-69 0.9601698042836091 1103 70-71 1.0198559200446233 1103 72-73 0.9898163304096492 1103 74-75 1.0345890451448447 1103 76-77 1.0286239254497183 1103 78-79 1.0179991301467517 1103 80-81 1.1361139332515897 1103 82-83 1.0432426474963847 1103 84-85 1.180608421916805 1103 86-87 1.0217887045203398 1103 88-89 0.9749300743707678 1103 90-91 1.2124498424503791 1103 92-93 1.3211788935067972 1103 94-95 1.386807256773828 1103 96-97 1.165606184834786 1103 98-99 1.38048168065297 1103 100-101 1.3145117496729135 1104 1 0.4049717062482152 1104 2 0.271561830542538 1104 3 0.19817625620635937 1104 4 0.1824120243371965 1104 5 0.23218294226038694 1104 6 0.25640953602780314 1104 7 0.2322734934528441 1104 8 0.21485598868150646 1104 9 0.30994632136389555 1104 10-11 0.3238601067146831 1104 12-13 0.1873061278156669 1104 14-15 0.2648448974830373 1104 16-17 0.4119355036648287 1104 18-19 0.4526950873259423 1104 20-21 0.4232071087805096 1104 22-23 0.2797560366010927 1104 24-25 0.4946257270265946 1104 26-27 0.44811066864300386 1104 28-29 0.4695929342287357 1104 30-31 0.4210559667868594 1104 32-33 0.3942184764526715 1104 34-35 0.441989971863741 1104 36-37 0.5072376489524189 1104 38-39 0.4877964831577515 1104 40-41 0.6168170185229371 1104 42-43 0.6262262508275356 1104 44-45 0.44898319407218423 1104 46-47 0.610692344730758 1104 48-49 0.6979448293443014 1104 50-51 0.5017433908384987 1104 52-53 0.5327467011709217 1104 54-55 0.4559568659361801 1104 56-57 0.49276238735368594 1104 58-59 0.7002726866274571 1104 60-61 0.5526033145430134 1104 62-63 0.5705098483433417 1104 64-65 0.5772879738693888 1104 66-67 0.5854999749777292 1104 68-69 0.7733798447307905 1104 70-71 0.7330358716040628 1104 72-73 0.8262766765664757 1104 74-75 0.7197841481146909 1104 76-77 0.6826963126918848 1104 78-79 0.8292026383582538 1104 80-81 0.887978480137317 1104 82-83 0.9748633761568328 1104 84-85 0.8241973604121355 1104 86-87 0.8421489489134188 1104 88-89 1.003398273472616 1104 90-91 1.2024626602437927 1104 92-93 0.9833872868875346 1104 94-95 1.1258938498276265 1104 96-97 1.100712603809633 1104 98-99 1.1486444357035914 1104 100-101 0.9652652634692345 1105 1 0.3213454459103424 1105 2 0.29941150884393153 1105 3 0.2734649033500247 1105 4 0.1914928469042465 1105 5 0.18963241171140766 1105 6 0.16203732242225044 1105 7 0.1845878132693386 1105 8 0.1510031981267943 1105 9 0.2394767463053924 1105 10-11 0.23577886145798033 1105 12-13 0.14249692418415805 1105 14-15 0.24262532917321522 1105 16-17 0.33091047613191193 1105 18-19 0.3636823529886115 1105 20-21 0.3152751683069752 1105 22-23 0.3353093141202592 1105 24-25 0.21096287003408776 1105 26-27 0.1569682208632912 1105 28-29 0.14603391469104565 1105 30-31 0.2573500070677923 1105 32-33 0.28652162933477143 1105 34-35 0.4363292792336111 1105 36-37 0.5097848922948813 1105 38-39 0.5062636129719991 1105 40-41 0.3271748285599827 1105 42-43 0.2642547538466715 1105 44-45 0.40565579616266945 1105 46-47 0.41570256684956064 1105 48-49 0.21167165018163558 1105 50-51 0.333672998147172 1105 52-53 0.3250705955743598 1105 54-55 0.3398210899555423 1105 56-57 0.48187360120424216 1105 58-59 0.42566673281988443 1105 60-61 0.3838609580669967 1105 62-63 0.3871354932696889 1105 64-65 0.3849160015078894 1105 66-67 0.48255351714777106 1105 68-69 0.5849579874727766 1105 70-71 0.6690580168514266 1105 72-73 0.6633976440576426 1105 74-75 0.5323612667654203 1105 76-77 0.5095664581251462 1105 78-79 0.7105944525306249 1105 80-81 0.7143089017984359 1105 82-83 0.6573762917689052 1105 84-85 0.4458475790865428 1105 86-87 0.6026672409191853 1105 88-89 0.7918385144843114 1105 90-91 0.7351739529599648 1105 92-93 0.8407930863809199 1105 94-95 1.0053931283165483 1105 96-97 0.8598475012655342 1105 98-99 0.891915144677494 1105 100-101 0.9763182390916256 1106 1 -0.00495248187829489 1106 2 0.052452729416295085 1106 3 0.10334714417774649 1106 4 0.01296760793383811 1106 5 0.023133972433718952 1106 6 0.06526670484297625 1106 7 0.13358379720508395 1106 8 0.025211897330386535 1106 9 -0.020706845203505964 1106 10-11 -0.5094320062258291 1106 12-13 -0.16761373637575616 1106 14-15 -0.018389675154018903 1106 16-17 -0.004008376032196281 1106 18-19 0.05988897122913528 1106 20-21 -0.09648664893779113 1106 22-23 -0.20611108904202524 1106 24-25 -0.13478914529112984 1106 26-27 -0.1362244043591474 1106 28-29 -0.20378424414283813 1106 30-31 0.052439323169970464 1106 32-33 -0.02895966630784841 1106 34-35 -0.11383505850474762 1106 36-37 -0.0856690785521863 1106 38-39 -0.09323170486107557 1106 40-41 0.23041150407561162 1106 42-43 0.04163157347385038 1106 44-45 0.2618646509522904 1106 46-47 0.3485550421259731 1106 48-49 0.2524624161510829 1106 50-51 0.4152793266087187 1106 52-53 0.33328845490514425 1106 54-55 0.05078698744380006 1106 56-57 0.2413755314433601 1106 58-59 0.33836790068748 1106 60-61 0.2773496829422797 1106 62-63 0.2531452951892277 1106 64-65 0.2641462396517511 1106 66-67 0.15225362508557794 1106 68-69 -0.04305867968010091 1106 70-71 0.24552952029863206 1106 72-73 0.4443757939453299 1106 74-75 0.5626407381619032 1106 76-77 0.5895055850930682 1106 78-79 0.7448073331086249 1106 80-81 0.4290735293157688 1106 82-83 0.417407700697602 1106 84-85 0.3400369272783408 1106 86-87 0.6267145196223787 1106 88-89 0.26463478430937215 1106 90-91 0.16521810049441044 1106 92-93 0.11779941678729244 1106 94-95 0.24764543050350696 1106 96-97 0.26620221795310783 1106 98-99 0.12098639335258454 1106 100-101 -5.835569545453723E-4 1107 1 0.21751544658913602 1107 2 0.09656539412341658 1107 3 0.10359254519780592 1107 4 0.08400570918922767 1107 5 0.10599710642450333 1107 6 0.11658298829947 1107 7 0.04784635798304038 1107 8 0.11177985418375869 1107 9 0.09301546706014108 1107 10-11 0.11390260905313454 1107 12-13 0.08680622901171375 1107 14-15 0.14430753844558808 1107 16-17 0.15439752458038924 1107 18-19 0.17035056322246334 1107 20-21 0.247866380574429 1107 22-23 0.26304758835635766 1107 24-25 0.1748071334038528 1107 26-27 0.24000237182229966 1107 28-29 0.25775051947554317 1107 30-31 0.16860465752886 1107 32-33 0.1889486562662981 1107 34-35 0.3068514233786104 1107 36-37 0.20248391250166975 1107 38-39 0.19728098182226006 1107 40-41 0.20719409525398902 1107 42-43 0.43055831028125624 1107 44-45 0.36160322561187996 1107 46-47 0.20667799902512485 1107 48-49 0.10270278095617869 1107 50-51 -0.05488842513335612 1107 52-53 0.052883909492223324 1107 54-55 0.06058746176368146 1107 56-57 0.13545202697027747 1107 58-59 0.09418335545769452 1107 60-61 0.03966872258187237 1107 62-63 0.036162720493003064 1107 64-65 0.02994576166916829 1107 66-67 0.08815954859004904 1107 68-69 0.08773594347366753 1107 70-71 0.10823589065272543 1107 72-73 0.27648532704242257 1107 74-75 0.35455395044294136 1107 76-77 0.39582740838601893 1107 78-79 0.27743788553562254 1107 80-81 0.20082082120563172 1107 82-83 0.16927384726061234 1107 84-85 0.17969986714593134 1107 86-87 0.3888664484927098 1107 88-89 0.5305730311571608 1107 90-91 0.5208101304553132 1107 92-93 0.5402554414739029 1107 94-95 0.5584165207982608 1107 96-97 0.6567094105837512 1107 98-99 0.7200212714303937 1107 100-101 0.4341979170997874 1108 1 0.33304873071456953 1108 2 0.21714667037328184 1108 3 0.29383040164861285 1108 4 0.3324104184948595 1108 5 0.27479404826601694 1108 6 0.20129066712591026 1108 7 0.23283633324351882 1108 8 0.11053379247428552 1108 9 0.29634245182443664 1108 10-11 0.30788499631699295 1108 12-13 0.14155838243421215 1108 14-15 0.12726173154419484 1108 16-17 0.34902702745657166 1108 18-19 0.2973131464699108 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-0.8521393632675967 2316 52-53 -1.013230097545609 2316 54-55 -0.9976097143427367 2316 56-57 -1.000352213250487 2316 58-59 -1.164498949213268 2316 60-61 -0.973845155964419 2316 62-63 -1.0380327472599973 2316 64-65 -1.003509127579889 2316 66-67 -1.252346313836398 2316 68-69 -1.1005140163727205 2316 70-71 -1.200267547956063 2316 72-73 -1.1020587762180512 2316 74-75 -1.1936121248706222 2316 76-77 -1.258287971175065 2316 78-79 -1.2398544473158672 2316 80-81 -1.023518469462669 2316 82-83 -1.2144635773601173 2316 84-85 -1.2489117671788286 2316 86-87 -1.2122914354852732 2316 88-89 -1.1615371503371392 2316 90-91 -1.0343543533153294 2316 92-93 -1.2209240524446514 2316 94-95 -1.420479474062077 2316 96-97 -1.315432382749293 2316 98-99 -1.188839534269956 2316 100-101 -0.9926243265376868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5852.0 3 1758.0 4 1761.0 5 1977.0 6 2222.0 7 2732.0 8 3193.0 9 4006.0 10 4959.0 11 6019.0 12 7680.0 13 9147.0 14 10806.0 15 12602.0 16 15266.0 17 19383.0 18 17686.0 19 14249.0 20 14125.0 21 14565.0 22 15795.0 23 17224.0 24 19073.0 25 21099.0 26 23642.0 27 27474.0 28 32015.0 29 37157.0 30 44407.0 31 53624.0 32 66999.0 33 85612.0 34 116532.0 35 170257.0 36 251131.0 37 340116.0 38 250706.0 39 29114.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81204079954403 18.349926698066927 14.804041389362288 23.03399111302676 2 15.634385009618267 19.382714446146455 40.6497285034405 24.33317204079478 3 19.432056492823772 23.508203904491623 29.493457316997425 27.566282285687183 4 12.88392546047902 16.526590238753933 36.50514253574194 34.08434176502511 5 13.85356568985431 36.76333127183285 34.19052218089808 15.192580857414764 6 32.52571990305249 35.64465009067643 17.141178398106245 14.688451608164835 7 28.835466242136775 32.43480279182241 20.175937445467806 18.553793520573002 8 26.2873107676463 35.05110282845587 19.25352208903597 19.408064314861857 9 26.0151602840083 17.063930098951204 20.01846336264698 36.90244625439352 10-11 25.079540246503463 26.456242345654363 26.767908521445154 21.696308886397013 12-13 26.113741264469763 23.7353042535041 27.324766817131525 22.826187664894608 14-15 22.851298643549555 25.29615622966348 25.80231594609207 26.050229180694895 16-17 22.202470881438405 28.368239655792106 26.637769978256898 22.791519484512595 18-19 22.17424055482681 26.707844630874945 29.177223731472317 21.940691082825925 20-21 22.69455340545233 25.817466411127967 29.75223344573158 21.735746737688128 22-23 22.508306140317934 25.706696046511563 29.50776995061916 22.277227862551342 24-25 22.3081146451555 25.92589559114014 29.55929476769506 22.2066949960093 26-27 21.880526307021462 26.295840024079624 29.452705188611983 22.37092848028692 28-29 22.016218749823523 26.559247567469885 29.35797017150537 22.066563511201213 30-31 22.093086953820784 26.154452168903997 29.363616854256204 22.388844023019015 32-33 21.911725215231524 26.186113532456908 29.32578720563706 22.57637404667451 34-35 22.496232372203508 26.326406884600324 29.10446939347758 22.072891349718596 36-37 22.523254891341637 25.963366504654932 29.065825820772083 22.447552783231348 38-39 22.08407186189932 26.321539176805643 29.07122252342049 22.52316643787454 40-41 22.611195078560222 26.15375885916184 29.000801156737083 22.234244905540855 42-43 22.25087778034348 26.1130830626644 28.953737771908656 22.682301385083463 44-45 22.30179238903546 26.406946932776098 28.98328744266489 22.307973235523555 46-47 22.554588813477615 26.67142166030378 28.31618468493134 22.457804841287267 48-49 22.505777674411252 26.612978796188933 28.2745894097232 22.60665411967661 50-51 22.54973850066186 26.970361526715834 28.110148658069505 22.3697513145528 52-53 22.76180885347194 26.769048918703653 28.000046287646107 22.4690959401783 54-55 22.498847056791412 26.869193301018935 28.16534419235552 22.46661544983413 56-57 22.481698796496712 26.976666865176078 28.095554339411017 22.446079998916197 58-59 22.516980384431502 26.718260242277758 27.991344561958858 22.773414811331886 60-61 22.34193407079746 26.89844513840436 28.143819052586654 22.615801738211523 62-63 22.317348376404926 26.88750547520241 28.372696463809476 22.422449684583185 64-65 22.175832828845152 26.901087859968193 28.241149583920727 22.681929727265928 66-67 22.119979014523608 26.876536482020253 28.125448897571065 22.878035605885078 68-69 22.325045303774473 26.663596085347486 28.319508661833325 22.691849949044716 70-71 22.39306380899391 26.705374198686055 27.98747650998754 22.914085482332492 72-73 22.327069057208377 26.832283829970294 27.88675525626738 22.95389185655395 74-75 22.22259857152857 27.154759727312346 27.79591781434388 22.826723886815202 76-77 22.44475471423334 26.832739088602718 27.83024046661265 22.89226573055129 78-79 22.475198374901968 26.93027662281916 27.823820905075525 22.77070409720335 80-81 22.52237709109669 26.990147193790293 27.628355717009523 22.85911999810349 82-83 22.466531534048734 27.101173915227704 27.636257311922098 22.79603723880146 84-85 22.468154016703053 27.069678469586577 27.630002957691463 22.832164556018903 86-87 22.615039424229316 27.012955348367058 27.465993233497937 22.90601199390569 88-89 22.62070203697258 27.01758635164291 27.364237819844554 22.997473791539953 90-91 22.57988198423012 27.04526648125714 27.33247150182563 23.04238003268711 92-93 22.526566024336276 26.75039947476231 27.5119207333682 23.21111376753321 94-95 22.447010250834758 26.76092085578618 27.528893554105487 23.26317533927358 96-97 22.715104644358235 26.447235186497846 27.500467899550223 23.3371922695937 98-99 22.52419807149323 26.581113324400512 27.259458142720565 23.63523046138569 100-101 23.026535020840406 25.96570059628403 27.365132321805135 23.64263206107043 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 875.0 1 589.5 2 466.0 3 622.5 4 499.0 5 427.0 6 802.0 7 1782.5 8 3022.0 9 3517.0 10 2843.5 11 2064.5 12 1901.0 13 1996.5 14 2103.0 15 2200.0 16 2345.0 17 2507.5 18 2703.5 19 2941.0 20 3262.0 21 3791.0 22 4507.0 23 5513.5 24 6844.0 25 8521.0 26 10608.5 27 13201.0 28 15514.0 29 17631.5 30 19818.0 31 22696.5 32 27127.5 33 29794.0 34 30906.5 35 33221.5 36 36755.5 37 40509.0 38 43524.0 39 45831.5 40 48178.5 41 51363.0 42 53912.5 43 56285.5 44 58911.5 45 61859.5 46 63673.5 47 65112.5 48 66259.5 49 65788.5 50 65149.5 51 63739.0 52 62707.5 53 63087.0 54 63564.0 55 60136.0 56 54776.5 57 50606.5 58 46994.5 59 41766.5 60 34381.5 61 28084.0 62 22697.5 63 17232.0 64 12809.0 65 9924.5 66 7463.0 67 5563.0 68 4188.0 69 3109.0 70 2320.5 71 1543.0 72 1008.0 73 738.0 74 514.5 75 356.0 76 281.0 77 206.0 78 117.5 79 62.5 80 37.0 81 28.5 82 22.5 83 14.0 84 11.5 85 7.5 86 6.5 87 6.0 88 4.5 89 5.5 90 2.5 91 1.0 92 1.5 93 1.0 94 1.0 95 1.0 96 1.5 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49199395250379097 2 0.04875920795632891 3 0.05570062297788962 4 0.06512514581204115 5 0.0800237926875861 6 0.10965178363327208 7 0.06941415021560712 8 0.05282247528602298 9 0.05067797308424 10-11 0.07497292565970248 12-13 0.10739441289455313 14-15 0.055898142917527524 16-17 0.060187147321093495 18-19 0.05880450774362815 20-21 0.07531153127051032 22-23 0.06458902026159541 24-25 0.09063343515956508 26-27 0.06667708819491042 28-29 0.06676173959761238 30-31 0.07127648107505023 32-33 0.07130469820928423 34-35 0.07316702906872734 36-37 0.06865228759128948 38-39 0.040322284820366906 40-41 0.04424446647889105 42-43 0.04198709574017211 44-45 0.020937113601618082 46-47 0.028330002770922583 48-49 0.028160699965518666 50-51 0.025846894958331756 52-53 0.024943946662844184 54-55 0.023702392756548774 56-57 0.024633558186270336 58-59 0.02088067933315011 60-61 0.028047831428582713 62-63 0.019695559695322672 64-65 0.021191067809723962 66-67 0.015321903889054743 68-69 0.018143617312453408 70-71 0.04184601006900218 72-73 0.03719018292039439 74-75 0.032111098758276786 76-77 0.022376187447551404 78-79 0.01492686400977893 80-81 0.016648109198052114 82-83 0.021445022017829843 84-85 0.018115400178219422 86-87 0.008831963015237818 88-89 0.04184601006900218 90-91 0.052963560957192915 92-93 0.03298582991953038 94-95 0.053415035104936705 96-97 0.0428618269014257 98-99 0.03400164675195389 100-101 0.1655499265507996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1771973.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.1826223491893 #Duplication Level Percentage of deduplicated Percentage of total 1 81.73106755145687 51.639831752997786 2 11.008412838853157 13.91080782122452 3 3.1420079861076475 5.955609120131291 4 1.3067085813964958 3.302450992752787 5 0.725756775135286 2.292760812036914 6 0.45049115560925024 1.707792753390548 7 0.2941291500610192 1.3008695707135385 8 0.22469589514026098 1.1357500708848118 9 0.15192471460795778 0.8639101681724665 >10 0.8845901581389571 9.980473602806585 >50 0.05155328527119289 2.225649217273128 >100 0.025169364898926124 2.903110924789927 >500 0.0024179146082750134 1.0100938343504287 >1k 8.955239289907458E-4 0.8841998851329906 >5k 1.7910478579814915E-4 0.8866894733422344 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8471 0.47805468819220154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7200 0.4063267329694076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4281 0.24159510331139356 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1970 0.11117550888190735 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.643426846797327E-5 2 0.0 0.0 0.0 0.0 5.643426846797327E-5 3 0.0 0.0 0.0 0.0 5.643426846797327E-5 4 0.0 0.0 0.0 0.0 5.643426846797327E-5 5 0.0 0.0 0.0 0.0 5.643426846797327E-5 6 0.0 0.0 0.0 0.0 5.643426846797327E-5 7 0.0 0.0 0.0 0.0 5.643426846797327E-5 8 0.0 0.0 0.0 0.0 5.643426846797327E-5 9 0.0 0.0 0.0 5.643426846797327E-5 5.643426846797327E-5 10-11 0.0 0.0 0.0 5.643426846797327E-5 5.643426846797327E-5 12-13 0.0 0.0 0.0 5.643426846797327E-5 5.643426846797327E-5 14-15 0.0 0.0 0.0 5.643426846797327E-5 8.46514027019599E-5 16-17 0.0 0.0 0.0 8.46514027019599E-5 1.1286853693594654E-4 18-19 0.0 0.0 0.0 1.1286853693594654E-4 1.1286853693594654E-4 20-21 0.0 0.0 0.0 1.693028054039198E-4 1.1286853693594654E-4 22-23 0.0 0.0 0.0 1.9751993963790644E-4 1.9751993963790644E-4 24-25 0.0 0.0 0.0 3.386056108078396E-4 2.539542081058797E-4 26-27 0.0 0.0 0.0 8.747311612535857E-4 2.8217134233986637E-4 28-29 0.0 0.0 0.0 0.00293458196033461 2.8217134233986637E-4 30-31 0.0 0.0 0.0 0.010129951190001202 2.8217134233986637E-4 32-33 0.0 0.0 0.0 0.024887512394376213 2.8217134233986637E-4 34-35 0.0 0.0 0.0 0.04636075154644004 2.8217134233986637E-4 36-37 0.0 0.0 0.0 0.08326876312449456 2.8217134233986637E-4 38-39 0.0 0.0 0.0 0.13662736396096328 2.8217134233986637E-4 40-41 0.0 0.0 0.0 0.19167899285147122 2.8217134233986637E-4 42-43 0.0 0.0 0.0 0.24994737504465364 3.1038847657385296E-4 44-45 0.0 0.0 0.0 0.31927687385755876 3.386056108078396E-4 46-47 0.0 0.0 0.0 0.4029688939955631 3.386056108078396E-4 48-49 0.0 0.0 0.0 0.49097813567136744 3.386056108078396E-4 50-51 0.0 0.0 0.0 0.5804828854615731 3.386056108078396E-4 52-53 0.0 0.0 0.0 0.6636105629148976 3.386056108078396E-4 54-55 0.0 0.0 0.0 0.7542722152086967 3.386056108078396E-4 56-57 0.0 0.0 0.0 0.8652219870167321 3.386056108078396E-4 58-59 0.0 0.0 0.0 0.9835646479940721 3.386056108078396E-4 60-61 0.0 0.0 0.0 1.1089615925299088 3.386056108078396E-4 62-63 0.0 0.0 0.0 1.2282918532054383 3.386056108078396E-4 64-65 0.0 0.0 0.0 1.3558897342115257 3.386056108078396E-4 66-67 0.0 0.0 0.0 1.4984709134958603 3.6682274504182624E-4 68-69 0.0 0.0 0.0 1.659308578629584 3.950398792758129E-4 70-71 0.0 0.0 0.0 1.8316306173965404 3.950398792758129E-4 72-73 0.0 0.0 0.0 1.9978577551689556 4.232570135097995E-4 74-75 0.0 0.0 0.0 2.1693614970431265 4.796912819777728E-4 76-77 0.0 0.0 0.0 2.3546916346919504 5.079084162117595E-4 78-79 0.0 0.0 0.0 2.5625672625937304 5.079084162117595E-4 80-81 0.0 0.0 0.0 2.783084166632336 5.361255504457461E-4 82-83 0.0 0.0 0.0 3.0098088402024183 6.772112216156792E-4 84-85 0.0 0.0 0.0 3.2376339820076265 6.772112216156792E-4 86-87 0.0 0.0 0.0 3.464245787040773 7.054283558496658E-4 88-89 0.0 0.0 0.0 3.7180589094754826 7.900797585516258E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3620 0.0 60.013985 1 GTATCAA 6565 0.0 43.5654 1 ATCAACG 8030 0.0 35.03596 3 TCAACGC 8135 0.0 34.758995 4 TATCAAC 8105 0.0 34.71175 2 CAACGCA 8295 0.0 34.03125 5 AACGCAG 8520 0.0 33.188316 6 ACGCAGA 9545 0.0 29.390356 7 CGCAGAG 9545 0.0 29.141287 8 GGACCGA 565 0.0 26.915928 6 TGGTATC 1505 0.0 26.208939 2 GCAGAGT 10545 0.0 25.69851 9 GTGGTAT 1595 0.0 24.846601 1 GAGTACT 6755 0.0 24.842323 12-13 CAGAGTA 10035 0.0 23.474686 10-11 GTACTTT 7235 0.0 23.047785 14-15 GTAAGGT 920 0.0 22.212042 4 AGGACCG 650 0.0 21.933895 5 GTACATG 5805 0.0 21.632322 1 TACATGG 5665 0.0 21.559618 2 >>END_MODULE