FastQCFastQC Report
Thu 26 May 2016
SRR938129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771973
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80840.4562146262950959No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72310.4080761952919147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44050.24859295260142225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18650.10524991069277015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37750.055.368451
GTATCAA63750.040.089321
ATCAACG80550.031.253613
TATCAAC81350.031.1240632
TCAACGC81900.030.7375744
TGGTATC16200.030.4961472
CAACGCA83650.030.094535
GTGGTAT17550.029.2329921
AACGCAG86400.029.1366586
ACGCAGA99000.025.3324017
CGCAGAG99350.025.0519228
GCAGAGT110700.022.1830129
GGACCGA5850.021.922276
CAGAGTA104050.021.40898910-11
GAGTACT72100.021.37690412-13
GTACTTT75100.020.42809914-15
TACATGG56050.020.340532
AGGACCG6800.020.2566075
GTACATG59250.020.2040671
AGAGTAC100550.019.9340610-11
Produced by FastQC (version 0.11.5)