FastQCFastQC Report
Thu 26 May 2016
SRR938128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737431
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78300.4506653789416673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67410.38798663083598717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40200.23137609493556868No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18200.10475236138874004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32950.057.2203671
GTATCAA58950.043.048131
TGGTATC14250.037.3820082
TATCAAC75400.033.3690452
ATCAACG75350.033.202783
TCAACGC76050.032.7720834
CAACGCA77900.031.99385
GTGGTAT16700.031.6458931
AACGCAG80650.030.9555976
GCGTATC650.005778362529.2993141
ACGCAGA93400.026.7244637
CGCAGAG94000.026.5500538
GCAGAGT102650.023.8489729
GAGTACT67500.022.85110912-13
CAGAGTA98250.021.35966710-11
TAAGGTG9150.021.3125175
GTACATG56200.021.1794431
GTACTTT73100.020.87265814-15
GTACCTG13500.020.8079411
TACATGG55450.020.671642
Produced by FastQC (version 0.11.5)