##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938128_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1737431 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.865012193289978 33.0 31.0 34.0 30.0 34.0 2 31.987395758450262 33.0 31.0 34.0 30.0 34.0 3 32.00395641611091 34.0 31.0 34.0 30.0 34.0 4 35.52048628118182 37.0 35.0 37.0 33.0 37.0 5 35.521262714893425 37.0 35.0 37.0 33.0 37.0 6 35.54623982189796 37.0 35.0 37.0 33.0 37.0 7 35.47803222113569 37.0 35.0 37.0 33.0 37.0 8 35.46533358734822 37.0 35.0 37.0 33.0 37.0 9 37.08177533381182 39.0 37.0 39.0 33.0 39.0 10-11 36.98264621731741 39.0 37.0 39.0 33.0 39.0 12-13 36.82413862766349 39.0 37.0 39.0 33.0 39.0 14-15 37.97611214488518 40.0 38.0 41.0 33.0 41.0 16-17 37.78598056555916 40.0 38.0 41.0 32.5 41.0 18-19 37.96908280098606 40.0 38.0 41.0 33.0 41.0 20-21 37.96184136233324 40.0 38.0 41.0 33.0 41.0 22-23 37.84298340480859 40.0 38.0 41.0 32.5 41.0 24-25 37.71568165872486 40.0 38.0 41.0 32.0 41.0 26-27 37.518115827333574 40.0 37.5 41.0 32.0 41.0 28-29 37.32714795580371 40.0 37.0 41.0 31.5 41.0 30-31 37.08905360845985 40.0 37.0 41.0 31.0 41.0 32-33 36.77862689223342 39.5 36.0 41.0 30.0 41.0 34-35 36.52175251851728 39.0 36.0 41.0 30.0 41.0 36-37 36.23915021661291 39.0 35.0 41.0 29.5 41.0 38-39 35.92318917988686 39.0 35.0 40.0 28.5 41.0 40-41 35.79170568500274 39.0 35.0 40.0 27.5 41.0 42-43 35.55680369465032 39.0 35.0 40.0 26.5 41.0 44-45 35.20308288501816 38.5 35.0 40.0 25.5 41.0 46-47 34.803775229059454 38.0 34.0 40.0 24.0 41.0 48-49 34.37871144235368 38.0 33.5 40.0 23.0 41.0 50-51 33.64021391353096 37.5 33.0 39.5 21.5 40.5 52-53 33.7032023142214 38.0 33.0 39.5 21.5 40.5 54-55 33.76713233503949 38.0 33.0 40.0 19.5 41.0 56-57 33.42416763600971 38.0 33.0 40.0 17.5 41.0 58-59 32.987043802027245 37.0 32.5 40.0 13.5 41.0 60-61 32.45307324434755 36.5 31.5 40.0 8.5 41.0 62-63 31.876064718541343 36.0 31.0 39.0 6.5 41.0 64-65 31.30987244961095 35.5 30.5 39.0 2.0 40.0 66-67 30.694517940568574 35.0 29.5 38.5 2.0 40.0 68-69 29.98990031834358 35.0 28.5 38.0 2.0 40.0 70-71 29.377436571581836 34.0 28.0 37.0 2.0 39.5 72-73 28.645765500903345 34.0 26.5 36.5 2.0 39.0 74-75 27.933712763269448 34.0 26.0 36.0 2.0 39.0 76-77 27.15657226099914 33.0 24.5 35.5 2.0 38.0 78-79 26.351282151636525 32.5 22.5 35.0 2.0 37.0 80-81 25.593786458282374 32.0 19.5 35.0 2.0 36.5 82-83 24.831281069579166 31.0 16.5 35.0 2.0 36.0 84-85 24.064365721573978 31.0 7.5 34.0 2.0 35.5 86-87 23.282809792158652 30.5 2.0 34.0 2.0 35.0 88-89 22.37344159278843 29.5 2.0 34.0 2.0 35.0 90-91 21.44784483527691 29.0 2.0 34.0 2.0 35.0 92-93 20.482057416956415 28.0 2.0 34.0 2.0 35.0 94-95 19.373144027014597 26.5 2.0 33.5 2.0 35.0 96-97 18.085433033024046 24.5 2.0 33.0 2.0 34.5 98-99 16.531706582880126 10.5 2.0 32.0 2.0 34.0 100-101 12.792327292421973 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1416743156259841 1101 2 0.19929326215358856 1101 3 0.23658539896045028 1101 4 0.007417939482088798 1101 5 0.09993261205635662 1101 6 0.10544702991271038 1101 7 0.18915226056864753 1101 8 0.18588042599786547 1101 9 0.09985422257766885 1101 10-11 0.17472002537012798 1101 12-13 0.018023644409780104 1101 14-15 0.22798607102785695 1101 16-17 0.34693937233710415 1101 18-19 0.18708310411550144 1101 20-21 0.3397077348041364 1101 22-23 0.34130241646879966 1101 24-25 0.23306544092892523 1101 26-27 0.2705652397983158 1101 28-29 0.0765801116463578 1101 30-31 0.10722677051547436 1101 32-33 0.10409844568680882 1101 34-35 0.19690922120508247 1101 36-37 0.1960567137394449 1101 38-39 0.2896351928329963 1101 40-41 0.31044747601589506 1101 42-43 0.27596006777474713 1101 44-45 0.2724043481525058 1101 46-47 0.389503376549051 1101 48-49 0.6358299843909592 1101 50-51 0.5853273200453017 1101 52-53 0.50352278852327 1101 54-55 0.29673327400634264 1101 56-57 0.14622937673473757 1101 58-59 0.3345775070919643 1101 60-61 0.19317210915702532 1101 62-63 -0.06001202329210642 1101 64-65 -0.22345948261686388 1101 66-67 -0.22842612179529098 1101 68-69 -0.1971235904888431 1101 70-71 -0.218609244312006 1101 72-73 -0.3258222456689879 1101 74-75 -0.3652628980833832 1101 76-77 -0.20824951179710993 1101 78-79 -0.33074711791270417 1101 80-81 -0.25285284271642894 1101 82-83 -0.3154062613107591 1101 84-85 -0.5556518279744118 1101 86-87 -0.40917575076864665 1101 88-89 -0.28804763409132406 1101 90-91 -0.329527208524091 1101 92-93 -0.29076295272735564 1101 94-95 -0.8560845435492332 1101 96-97 -0.9741203641323644 1101 98-99 -1.1483207415361072 1101 100-101 -1.4205118580144624 1102 1 0.22799238637552577 1102 2 0.30695448632620526 1102 3 0.25403620497338153 1102 4 0.20496061456432813 1102 5 0.24981302684865625 1102 6 0.23006863123203658 1102 7 0.26148792804406185 1102 8 0.3209219138623425 1102 9 0.29841833665417994 1102 10-11 0.2939400017121159 1102 12-13 0.2036134110243566 1102 14-15 0.2205929422125692 1102 16-17 0.23995853187327043 1102 18-19 0.3667890194819847 1102 20-21 0.28405269279821255 1102 22-23 0.3937135478225784 1102 24-25 0.26945301788221343 1102 26-27 0.3673282365949859 1102 28-29 0.2639744492651843 1102 30-31 0.3596331529981853 1102 32-33 0.4071208862611684 1102 34-35 0.3085016471874482 1102 36-37 0.4300914409383907 1102 38-39 0.2256260231946854 1102 40-41 0.3664608419293671 1102 42-43 0.44459550126033065 1102 44-45 0.48071400908310835 1102 46-47 0.2793442461968567 1102 48-49 0.09866575116095078 1102 50-51 0.12679964276130562 1102 52-53 0.19932737679614831 1102 54-55 0.37350870421089155 1102 56-57 0.28310824598537465 1102 58-59 0.2810939943088684 1102 60-61 0.23959664096150846 1102 62-63 -0.09076787087924387 1102 64-65 -4.4904373483589666E-4 1102 66-67 0.004639403991077984 1102 68-69 -0.08294691521664532 1102 70-71 -0.42537250013800687 1102 72-73 -0.4433991126228882 1102 74-75 -0.32098485599975035 1102 76-77 -0.2939385729884698 1102 78-79 -0.4270382496118508 1102 80-81 -0.5228071672111057 1102 82-83 -0.2484558136217352 1102 84-85 -0.1616077757195633 1102 86-87 -0.24333222175304314 1102 88-89 -0.31684435650685927 1102 90-91 -0.3184506826254747 1102 92-93 -0.5571867074438472 1102 94-95 -0.8883466466947176 1102 96-97 -1.121479128389769 1102 98-99 -1.1758408810665735 1102 100-101 -1.1824013042960946 1103 1 0.2820341488616549 1103 2 0.31748473761317797 1103 3 0.4021896245559944 1103 4 0.26842097041105006 1103 5 0.3180503166440616 1103 6 0.2720697150881364 1103 7 0.3178849612991925 1103 8 0.4365003120956459 1103 9 0.3498923726571306 1103 10-11 0.4401796600567778 1103 12-13 0.3484627707940362 1103 14-15 0.37607898860260747 1103 16-17 0.4392737356523213 1103 18-19 0.5056647221004198 1103 20-21 0.5429258310097822 1103 22-23 0.3112610204709796 1103 24-25 0.39543365453563695 1103 26-27 0.36558170124143885 1103 28-29 0.44130899315347705 1103 30-31 0.6871168308389741 1103 32-33 0.5836900501400422 1103 34-35 0.44115783325882063 1103 36-37 0.31417589374459 1103 38-39 0.3173485908879812 1103 40-41 0.5693164210081179 1103 42-43 0.4653933388595206 1103 44-45 0.40725391880602757 1103 46-47 0.446976576645163 1103 48-49 0.3708155042226764 1103 50-51 0.45501571188244583 1103 52-53 0.3957150902166262 1103 54-55 0.19762400459090657 1103 56-57 0.4115408373558509 1103 58-59 0.36227572942473074 1103 60-61 0.43987349731406056 1103 62-63 0.1846716261318484 1103 64-65 0.2855045214131344 1103 66-67 0.0698014803141298 1103 68-69 -0.022076693291712246 1103 70-71 -0.06055346510190063 1103 72-73 -0.3464072697604479 1103 74-75 -0.2953293761776514 1103 76-77 -0.2600946266899733 1103 78-79 -0.41200763026194664 1103 80-81 -0.4503307219935593 1103 82-83 -0.42067834936367277 1103 84-85 -0.2975286011100131 1103 86-87 -0.4270082782430258 1103 88-89 -0.527335892719357 1103 90-91 -0.6436855250306408 1103 92-93 -0.7542240291076041 1103 94-95 -0.6612482938074322 1103 96-97 -1.0054021646474958 1103 98-99 -1.4561440371128072 1103 100-101 -1.6481413680494068 1104 1 0.17880622449349204 1104 2 0.17592285938795982 1104 3 0.1971085361173479 1104 4 0.026769016161665604 1104 5 0.11233629400896206 1104 6 0.08986953813879239 1104 7 0.1819466293470242 1104 8 0.20486080835448206 1104 9 0.13968095421996907 1104 10-11 0.19722200379488442 1104 12-13 0.2611991202729982 1104 14-15 0.1971872039363518 1104 16-17 0.18235109294187168 1104 18-19 0.2820912260697668 1104 20-21 0.26355504206390634 1104 22-23 0.28354282631510586 1104 24-25 0.27566196723155656 1104 26-27 0.08772338882576491 1104 28-29 0.09516064394674828 1104 30-31 0.08697811376176645 1104 32-33 0.04484566547939295 1104 34-35 0.051694926565566846 1104 36-37 0.11358139198510742 1104 38-39 0.054121427161788915 1104 40-41 0.01469171775858058 1104 42-43 0.17163460648939832 1104 44-45 0.16956791181613085 1104 46-47 -0.04851361707834201 1104 48-49 -0.06544870276802328 1104 50-51 -0.06924596497281499 1104 52-53 0.08520882292703646 1104 54-55 0.09374272879412615 1104 56-57 0.10403270048833235 1104 58-59 0.11656134648552552 1104 60-61 0.06448286762260125 1104 62-63 0.025041498303963294 1104 64-65 -0.28226867916815834 1104 66-67 0.03619131333320169 1104 68-69 0.3635619857533996 1104 70-71 0.02253366043204963 1104 72-73 -0.1104405138184319 1104 74-75 -0.40473351326935614 1104 76-77 -0.5532118175990988 1104 78-79 -0.5041445938592197 1104 80-81 -0.5733139198125166 1104 82-83 -0.2503077565833678 1104 84-85 -0.39639904776699453 1104 86-87 -0.3733183639553701 1104 88-89 -0.41660983993080336 1104 90-91 -0.6186437898061108 1104 92-93 -0.5674800171615217 1104 94-95 -1.0202146614217007 1104 96-97 -1.2953957192075443 1104 98-99 -1.1222534334433085 1104 100-101 -1.122583997628773 1105 1 0.1261262282483031 1105 2 0.21282889524640325 1105 3 0.15907230219263369 1105 4 0.1386623795334856 1105 5 0.15503788041654332 1105 6 0.28166527642847683 1105 7 0.20511944451646258 1105 8 0.19725731638039434 1105 9 0.28238066322212774 1105 10-11 0.2094655032786008 1105 12-13 0.2959123465942213 1105 14-15 0.2368473935542994 1105 16-17 0.251568214879363 1105 18-19 0.27557475183715496 1105 20-21 0.30854236957728887 1105 22-23 0.3406253382835729 1105 24-25 0.18896244596994194 1105 26-27 0.31802001888301845 1105 28-29 0.22792042992252703 1105 30-31 0.032303374251760886 1105 32-33 0.02234496145235454 1105 34-35 -0.04577339221787469 1105 36-37 0.03243805208085604 1105 38-39 0.08538492101328643 1105 40-41 0.0446898403531506 1105 42-43 -0.0109599678089225 1105 44-45 0.1678585341760268 1105 46-47 0.1338969714882623 1105 48-49 0.0881399577032127 1105 50-51 -0.020865227152491173 1105 52-53 0.11509242379064233 1105 54-55 0.08498275507780306 1105 56-57 0.15678685424784078 1105 58-59 0.09536825533749749 1105 60-61 -0.04792208872772363 1105 62-63 0.14802000201183674 1105 64-65 0.1679875866724494 1105 66-67 -0.15363221055678267 1105 68-69 -0.15644552386829957 1105 70-71 -0.16783431103138824 1105 72-73 -0.4194013699153061 1105 74-75 -0.38226003162099076 1105 76-77 -0.6771365336415265 1105 78-79 -0.7761637433945623 1105 80-81 -0.534871227613138 1105 82-83 -0.49819661418216654 1105 84-85 -0.5516595377698081 1105 86-87 -0.6059504863622003 1105 88-89 -0.6373072960464512 1105 90-91 -0.5293759779221361 1105 92-93 -0.6658504292520178 1105 94-95 -0.7246631735778983 1105 96-97 -0.778908344759035 1105 98-99 -1.1682367245350207 1105 100-101 -1.2629829462817366 1106 1 0.3291921404311111 1106 2 0.25820478988154605 1106 3 0.22334998905554215 1106 4 0.2728330059259605 1106 5 0.26670017659746037 1106 6 0.2952499291227326 1106 7 0.2309799701577404 1106 8 -0.006088569776665054 1106 9 0.3833854148930982 1106 10-11 0.3522721437880776 1106 12-13 0.4856804808239943 1106 14-15 0.4684262890372537 1106 16-17 -0.03264105580815624 1106 18-19 0.43033154909262805 1106 20-21 0.5459632572130886 1106 22-23 0.3975990562196472 1106 24-25 0.4019138054210316 1106 26-27 0.30804150984302225 1106 28-29 0.34099698900295294 1106 30-31 0.30974673867140723 1106 32-33 0.37119844831132554 1106 34-35 0.3534683120744049 1106 36-37 0.39828142438708625 1106 38-39 0.3709110552036279 1106 40-41 0.28368883481282126 1106 42-43 0.1627045366174329 1106 44-45 0.5111259487543904 1106 46-47 0.6368631182971498 1106 48-49 0.5976849014126699 1106 50-51 0.4109128047487687 1106 52-53 0.4317883365649493 1106 54-55 0.4291389884420269 1106 56-57 0.18090266883409356 1106 58-59 0.21370083287254005 1106 60-61 0.4663684138452737 1106 62-63 0.2536516193544536 1106 64-65 0.24969089312565274 1106 66-67 0.1416776338802137 1106 68-69 0.15163764023193593 1106 70-71 0.11756701785916235 1106 72-73 0.09245572505896504 1106 74-75 0.23727150488406323 1106 76-77 -0.13415737395778038 1106 78-79 -0.13922472645811013 1106 80-81 0.06990035700012598 1106 82-83 -0.10473893560017444 1106 84-85 -0.013438752659691744 1106 86-87 -0.18599169379925584 1106 88-89 -0.5096101805670443 1106 90-91 -0.4507853117023757 1106 92-93 -0.5661585977884585 1106 94-95 -0.8397360259606366 1106 96-97 -0.5762282840322825 1106 98-99 -0.6999737461639945 1106 100-101 -1.2015816962570884 1107 1 -0.03135446480119697 1107 2 -0.05148705849694579 1107 3 0.09226425129430993 1107 4 0.06648439545922002 1107 5 0.09236731901443562 1107 6 -0.002949184777556013 1107 7 0.04909047408710876 1107 8 0.056982817560815135 1107 9 0.11319335579145218 1107 10-11 0.07853196720561328 1107 12-13 -0.05717476476586825 1107 14-15 -0.02852814163837536 1107 16-17 0.0577848528690339 1107 18-19 0.05273212849274955 1107 20-21 0.10507712208819697 1107 22-23 0.20909798843762673 1107 24-25 -0.06955782132628485 1107 26-27 -0.1457389343401374 1107 28-29 -0.10088113864215842 1107 30-31 -0.17053364707591356 1107 32-33 -0.14359077541291043 1107 34-35 -0.11598426813320373 1107 36-37 -0.16644794687876896 1107 38-39 -0.049863768514946116 1107 40-41 -0.13271162417407822 1107 42-43 0.008741524728392847 1107 44-45 -0.15451271947529222 1107 46-47 -0.32900327259961415 1107 48-49 -0.14424209983750558 1107 50-51 -0.23927985641902438 1107 52-53 -0.18503162086543057 1107 54-55 -0.3036555547137212 1107 56-57 -0.4233611990513495 1107 58-59 -0.44197558844626883 1107 60-61 -0.3779649041412725 1107 62-63 -0.2670766327766856 1107 64-65 -0.3962299316341422 1107 66-67 -0.47129937073444594 1107 68-69 -0.4220299342560381 1107 70-71 -0.5407733690922889 1107 72-73 -0.468870378758492 1107 74-75 -0.7340074606954623 1107 76-77 -0.9706085835795051 1107 78-79 -0.7915997003390665 1107 80-81 -0.6050428894113864 1107 82-83 -0.8892846058591708 1107 84-85 -0.9845041618344723 1107 86-87 -1.0061281503212243 1107 88-89 -0.850208740566476 1107 90-91 -0.8613090738366651 1107 92-93 -0.9707089783296823 1107 94-95 -1.1892832368006587 1107 96-97 -1.1008554457153785 1107 98-99 -1.167615700966536 1107 100-101 -1.333126597996353 1108 1 0.014855441632111166 1108 2 -0.04361971790753927 1108 3 0.011985442468635199 1108 4 0.05721983458456492 1108 5 2.6569931880260356E-4 1108 6 0.09850997056683042 1108 7 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4095.0 9 5178.0 10 6398.0 11 7924.0 12 9712.0 13 11942.0 14 13617.0 15 16282.0 16 18865.0 17 22921.0 18 22890.0 19 20052.0 20 20673.0 21 22127.0 22 24576.0 23 27058.0 24 30010.0 25 33589.0 26 37827.0 27 42343.0 28 47991.0 29 55180.0 30 64122.0 31 75150.0 32 89799.0 33 110759.0 34 139329.0 35 178728.0 36 218453.0 37 224555.0 38 110092.0 39 6531.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80157429118853 18.362753303406123 14.760766763734123 23.07490564167122 2 15.656904483709189 19.38514198709957 40.725273548240715 24.23267998095053 3 19.482653166518418 23.598242229046342 29.405250599960688 27.51385400447456 4 12.94439180481687 16.521023959867858 36.555743060214354 33.97884117510092 5 13.8953354249331 36.72649257174495 34.17354895266217 15.204623050659777 6 32.54054951938106 35.62174776186032 17.181564719466884 14.656137999291733 7 28.82433896417108 32.46217648724053 20.153456135910385 18.560028412678005 8 26.324289703348047 34.96390918893015 19.275695551603064 19.436105556118736 9 26.119689840281 17.079646272695054 20.078145035110516 36.72251885191343 10-11 25.130625345162912 26.40791176044391 26.762343832239523 21.699119062153667 12-13 26.11108260838737 23.72517404755907 27.302624562827464 22.861118781226093 14-15 22.890493690819007 25.23372673972236 25.822366525028066 26.053413044430563 16-17 22.24530600858567 28.3715296509939 26.619784389942502 22.76337995047793 18-19 22.14976860984066 26.676624075354315 29.172767572698643 22.000839742106383 20-21 22.6535155658984 25.835515176841 29.728135334337196 21.782833922923405 22-23 22.531328515935105 25.73911132757587 29.44498416452629 22.284575991962736 24-25 22.315791598946085 25.88864068022953 29.51839668422715 22.277171036597238 26-27 21.928482262087208 26.198949511346665 29.41944692403629 22.453121302529837 28-29 22.07842954187479 26.533610890667426 29.29336415510706 22.094595412350728 30-31 22.098523586604248 26.1667410875045 29.29374144760533 22.44099387828592 32-33 21.90535963442656 26.144621433350796 29.290223192750776 22.659795739471868 34-35 22.50406746694751 26.332153038155838 29.075417520297 22.08836197459965 36-37 22.594706292898564 25.941503704085626 29.0606321328384 22.40315787017741 38-39 22.152645169315253 26.337787442039385 28.958626543600896 22.55094084504447 40-41 22.65998333871336 26.12899222252522 28.9590784862936 22.251945952467818 42-43 22.247438402735902 26.13647031449866 28.92590589632435 22.690185386441087 44-45 22.33245090883965 26.45479841710608 28.88081799167095 22.331932682383325 46-47 22.664594692336575 26.664806611890068 28.21104193012644 22.45955676564692 48-49 22.531056848399277 26.635271044203396 28.19542637739009 22.638245730007245 50-51 22.653525991820626 26.88301153998654 28.05843332956125 22.405029138631587 52-53 22.789952309713108 26.7067621643048 27.965443756867337 22.53784176911476 54-55 22.57767197019497 26.81200044572788 28.085002725332288 22.52532485874486 56-57 22.54417559236631 26.98623204592926 27.985781826807266 22.483810534897163 58-59 22.615046617563216 26.67803071749008 27.882629702787842 22.82429296215886 60-61 22.4229104313469 26.87416582505444 28.00870132995844 22.694222413640222 62-63 22.38595858766971 26.872577511706986 28.235750420322685 22.505713480300624 64-65 22.22434310648317 26.893925653121993 28.133030689867027 22.74870055052781 66-67 22.208140752764262 26.82523770396938 28.03763878900386 22.928982754262492 68-69 22.431959829906052 26.604854116737712 28.200449598178107 22.762736455178125 70-71 22.421761734120192 26.672435165575596 27.865506632094068 23.040296468210148 72-73 22.41867062174076 26.769396088173735 27.753644032390373 23.058289257695133 74-75 22.29212457007225 27.118860735367196 27.65714496302895 22.931869731531602 76-77 22.56164274338237 26.746507215844375 27.65886097974981 23.032989061023446 78-79 22.58433890828828 26.848325973525416 27.636109910942537 22.931225207243767 80-81 22.637110080704126 26.932742339786365 27.414790882011236 23.015356697498277 82-83 22.56659546301992 26.975138403827437 27.445825116513095 23.012441016639546 84-85 22.61617024265237 26.92537189704408 27.426127909958904 23.032329950344646 86-87 22.737475870573583 26.903310157185977 27.241568301892876 23.11764567034756 88-89 22.753673646934214 26.89832089417921 27.11729838678325 23.23070707210332 90-91 22.70053064566595 26.900491116626224 27.09035727682947 23.30862096087835 92-93 22.692364189370178 26.540598456766123 27.238999634209215 23.528037719654485 94-95 22.602523718361716 26.52395568213925 27.22217886068341 23.651341738815628 96-97 22.834859278236507 26.261813209598937 27.22933293092008 23.67399458124447 98-99 22.651271420825623 26.35736616096246 26.950021120999573 24.041341297212345 100-101 23.581699361472943 25.243662133368527 26.883954522165173 24.29068398299336 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 827.0 1 570.0 2 466.0 3 607.5 4 446.5 5 381.0 6 747.5 7 1696.5 8 2863.5 9 3248.5 10 2573.5 11 1880.5 12 1741.0 13 1775.0 14 1943.0 15 2139.5 16 2260.5 17 2395.5 18 2641.5 19 2953.5 20 3260.5 21 3747.5 22 4487.5 23 5480.5 24 6757.0 25 8470.5 26 10634.0 27 13032.5 28 15230.0 29 17261.5 30 19398.5 31 22326.5 32 26315.5 33 28987.5 34 30470.0 35 32460.5 36 35633.5 37 39378.5 38 42267.0 39 44364.5 40 46678.0 41 49747.5 42 52106.0 43 54433.0 44 57196.0 45 60022.5 46 61743.5 47 63226.5 48 64329.0 49 64063.0 50 63425.5 51 61965.5 52 61267.5 53 61656.0 54 61862.5 55 58913.0 56 54132.5 57 49990.0 58 46475.5 59 41715.0 60 34590.0 61 28492.5 62 23379.5 63 18090.0 64 13746.0 65 10646.5 66 7915.5 67 5867.5 68 4505.0 69 3370.5 70 2493.0 71 1726.0 72 1169.5 73 841.0 74 603.0 75 429.0 76 320.0 77 214.0 78 123.0 79 73.0 80 49.0 81 36.0 82 25.5 83 15.0 84 12.5 85 10.0 86 4.0 87 2.5 88 4.0 89 4.5 90 4.0 91 3.5 92 1.5 93 1.5 94 3.0 95 2.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14705619964188507 2 0.05180061826915716 3 0.1573587670531952 4 0.13600540107779818 5 0.20207996749223422 6 0.2086413791396608 7 0.17232339010872952 8 0.0722330843642136 9 0.12639350857674347 10-11 0.10575959563286254 12-13 0.15563207977755664 14-15 0.14383305006069305 16-17 0.14084012544958618 18-19 0.136667297866793 20-21 0.14207758466379383 22-23 0.20187852064340972 24-25 0.22438301146923242 26-27 0.15623642032403015 28-29 0.13203402034382947 30-31 0.10394657399344204 32-33 0.13203402034382947 34-35 0.09824850598383475 36-37 0.09079497257732826 38-39 0.05220351196680616 40-41 0.06161395761903638 42-43 0.06400254168366974 44-45 0.04250528510196952 46-47 0.05312441184714674 48-49 0.044721200439039016 50-51 0.05700945821733352 52-53 0.028461561926775795 54-55 0.05441942730387567 56-57 0.028720565018121584 58-59 0.055311549062955594 60-61 0.03942602612708073 62-63 0.055685664639343956 64-65 0.042246282010623734 66-67 0.040980044675155446 68-69 0.06987327842084087 70-71 0.05689434573229095 72-73 0.05338341493849252 74-75 0.04616010650207116 76-77 0.02218793149195565 78-79 0.02339661258490265 80-81 0.026993877742483012 82-83 0.022705937674647222 84-85 0.02057635670135965 86-87 0.025554971679450866 88-89 0.06788758805385653 90-91 0.1149973725575289 92-93 0.08437745153620489 94-95 0.07951394904315624 96-97 0.07131218448387303 98-99 0.05798791434019538 100-101 0.13491183246989377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1737431.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.99027761461308 #Duplication Level Percentage of deduplicated Percentage of total 1 82.44923340538172 52.759493347224065 2 10.550839086908905 13.50302244476823 3 3.0219632113429498 5.8012879450494905 4 1.283254134797416 3.28463153343147 5 0.6775629632573049 2.167872106010741 6 0.4279646818494397 1.6431347280477122 7 0.2906762539916589 1.3020317932231427 8 0.21457021446123176 1.0984326072961026 9 0.15534796303584225 0.8946683353375376 >10 0.851540672343948 9.834249351580995 >50 0.04907866364418184 2.1543799802692893 >100 0.024372057834653685 2.8124482932308332 >500 0.0026076010842194415 1.093458163079015 >1k 8.092555088956888E-4 0.8124985645568705 >5k 1.7983455753237525E-4 0.8383908068945657 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7830 0.4506653789416673 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6741 0.38798663083598717 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4020 0.23137609493556868 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1820 0.10475236138874004 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.755624252128574E-5 2 0.0 0.0 0.0 0.0 5.755624252128574E-5 3 0.0 0.0 0.0 0.0 5.755624252128574E-5 4 0.0 0.0 0.0 5.755624252128574E-5 5.755624252128574E-5 5 0.0 0.0 0.0 5.755624252128574E-5 5.755624252128574E-5 6 0.0 0.0 0.0 5.755624252128574E-5 5.755624252128574E-5 7 0.0 0.0 0.0 5.755624252128574E-5 5.755624252128574E-5 8 0.0 0.0 0.0 5.755624252128574E-5 5.755624252128574E-5 9 0.0 0.0 0.0 5.755624252128574E-5 1.1511248504257147E-4 10-11 0.0 0.0 0.0 5.755624252128574E-5 1.726687275638572E-4 12-13 0.0 0.0 0.0 5.755624252128574E-5 2.3022497008514295E-4 14-15 0.0 0.0 0.0 5.755624252128574E-5 2.3022497008514295E-4 16-17 0.0 0.0 0.0 8.63343637819286E-5 2.877812126064287E-4 18-19 0.0 0.0 0.0 1.726687275638572E-4 2.877812126064287E-4 20-21 0.0 0.0 0.0 1.726687275638572E-4 2.877812126064287E-4 22-23 0.0 0.0 0.0 2.014468488245001E-4 3.165593338670716E-4 24-25 0.0 0.0 0.0 4.892280614309288E-4 3.453374551277144E-4 26-27 0.0 0.0 0.0 8.92121759079929E-4 3.453374551277144E-4 28-29 0.0 0.0 0.0 0.0030217027323675015 3.453374551277144E-4 30-31 0.0 0.0 0.0 0.00984211747113986 3.453374551277144E-4 32-33 0.0 0.0 0.0 0.02466284992037094 3.453374551277144E-4 34-35 0.0 0.0 0.0 0.047196118867454305 3.453374551277144E-4 36-37 0.0 0.0 0.0 0.08408967032359846 3.453374551277144E-4 38-39 0.0 0.0 0.0 0.13971777872042113 3.453374551277144E-4 40-41 0.0 0.0 0.0 0.1968423494227972 3.453374551277144E-4 42-43 0.0 0.0 0.0 0.2567583978874557 3.741155763883573E-4 44-45 0.0 0.0 0.0 0.3295958227981428 4.028936976490002E-4 46-47 0.0 0.0 0.0 0.4120163620886239 4.028936976490002E-4 48-49 0.0 0.0 0.0 0.5002213037524943 4.604499401702859E-4 50-51 0.0 0.0 0.0 0.5957934444590893 4.604499401702859E-4 52-53 0.0 0.0 0.0 0.6844588360631301 4.604499401702859E-4 54-55 0.0 0.0 0.0 0.7800021986484643 5.180061826915716E-4 56-57 0.0 0.0 0.0 0.8918051997460619 5.180061826915716E-4 58-59 0.0 0.0 0.0 1.0106012843099956 5.180061826915716E-4 60-61 0.0 0.0 0.0 1.1357573336725313 5.180061826915716E-4 62-63 0.0 0.0 0.0 1.2591866957594289 5.180061826915716E-4 64-65 0.0 0.0 0.0 1.3854650918511298 5.180061826915716E-4 66-67 0.0 0.0 0.0 1.5347372068300842 5.467843039522146E-4 68-69 0.0 0.0 0.0 1.6950025641306044 5.755624252128574E-4 70-71 0.0 0.0 0.0 1.8620020018061147 6.331186677341432E-4 72-73 0.0 0.0 0.0 2.032685039002988 6.331186677341432E-4 74-75 0.0 0.0 0.0 2.204001194867595 6.331186677341432E-4 76-77 0.0 0.0 0.0 2.3878933897231027 6.331186677341432E-4 78-79 0.0 0.0 0.0 2.5895992416389486 6.331186677341432E-4 80-81 0.0 5.755624252128574E-5 0.0 2.8083129632198345 6.61896788994786E-4 82-83 0.0 5.755624252128574E-5 0.0 3.0289260408039222 7.482311527767146E-4 84-85 0.0 5.755624252128574E-5 0.0 3.250057124570702 7.482311527767146E-4 86-87 0.0 5.755624252128574E-5 0.0 3.475735151496664 7.770092740373574E-4 88-89 0.0 5.755624252128574E-5 0.0 3.7175001482073244 8.057873952980004E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3295 0.0 57.220367 1 GTATCAA 5895 0.0 43.04813 1 TGGTATC 1425 0.0 37.382008 2 TATCAAC 7540 0.0 33.369045 2 ATCAACG 7535 0.0 33.20278 3 TCAACGC 7605 0.0 32.772083 4 CAACGCA 7790 0.0 31.9938 5 GTGGTAT 1670 0.0 31.645893 1 AACGCAG 8065 0.0 30.955597 6 GCGTATC 65 0.0057783625 29.299314 1 ACGCAGA 9340 0.0 26.724463 7 CGCAGAG 9400 0.0 26.550053 8 GCAGAGT 10265 0.0 23.848972 9 GAGTACT 6750 0.0 22.851109 12-13 CAGAGTA 9825 0.0 21.359667 10-11 TAAGGTG 915 0.0 21.312517 5 GTACATG 5620 0.0 21.179443 1 GTACTTT 7310 0.0 20.872658 14-15 GTACCTG 1350 0.0 20.807941 1 TACATGG 5545 0.0 20.67164 2 >>END_MODULE