FastQCFastQC Report
Thu 26 May 2016
SRR938128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737431
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77080.4436435173540704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69040.3973682983669567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41190.23707416294517594No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.10469480514621876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.056.767921
GTATCAA59750.039.354541
TATCAAC78250.030.0494062
ATCAACG77300.030.0448063
TCAACGC78400.029.6812764
CAACGCA80200.029.0151145
AACGCAG83550.027.8517286
TGGTATC15500.027.5820082
GTGGTAT18200.025.0569041
ACGCAGA94900.024.3705487
CGCAGAG96600.023.9416688
GTACATG59850.022.7001881
TACATGG57850.022.4989512
GAGTACT68750.022.00210812-13
ACATGGG57400.021.8439983
GCAGAGT107450.021.3473159
GTACTTT72350.020.71039414-15
CAGAGTA102200.020.47022210-11
TACCGTG1908.27021E-619.9958087
AGAGTAC98100.019.77655410-11
Produced by FastQC (version 0.11.5)