##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938128_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1737431 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.503374810280235 34.0 31.0 34.0 31.0 34.0 2 32.71808491963134 34.0 31.0 34.0 31.0 34.0 3 32.77307185148648 34.0 31.0 34.0 31.0 34.0 4 36.23175826838591 37.0 35.0 37.0 35.0 37.0 5 36.2045491302964 37.0 35.0 37.0 35.0 37.0 6 36.23542978109634 37.0 37.0 37.0 35.0 37.0 7 36.19306032872672 37.0 37.0 37.0 35.0 37.0 8 36.17887731944463 37.0 37.0 37.0 35.0 37.0 9 37.8677841019298 39.0 38.0 39.0 35.0 39.0 10-11 37.843907470282275 39.0 38.0 39.0 35.0 39.0 12-13 37.76304612960169 39.0 38.0 39.0 35.0 39.0 14-15 39.03151175499919 40.0 38.0 41.0 35.5 41.0 16-17 38.90881968837899 40.0 38.0 41.0 35.0 41.0 18-19 39.16262545102511 40.0 38.5 41.0 36.0 41.0 20-21 39.14343533642487 40.0 39.0 41.0 36.0 41.0 22-23 39.04914497323922 40.0 39.0 41.0 35.5 41.0 24-25 38.95301626366745 40.0 38.0 41.0 35.0 41.0 26-27 38.77792441829345 40.0 38.0 41.0 35.0 41.0 28-29 38.657681082011315 40.0 38.0 41.0 34.5 41.0 30-31 38.420625049282535 40.0 38.0 41.0 34.0 41.0 32-33 38.20360751016875 40.0 38.0 41.0 34.0 41.0 34-35 38.04666717699868 40.0 38.0 41.0 33.0 41.0 36-37 38.04471947375176 40.0 38.0 41.0 33.0 41.0 38-39 37.89541340058972 40.0 37.5 41.0 33.0 41.0 40-41 37.72317001365809 40.0 37.0 41.0 33.0 41.0 42-43 37.489828660821644 40.0 37.0 41.0 32.5 41.0 44-45 37.21355438000128 40.0 37.0 41.0 32.0 41.0 46-47 36.82116642329969 40.0 36.0 41.0 31.0 41.0 48-49 36.41818437681842 39.0 36.0 41.0 30.0 41.0 50-51 36.03791575032332 39.0 35.0 40.5 29.0 41.0 52-53 35.513956525467776 38.5 35.0 40.0 28.0 41.0 54-55 35.19224619567626 38.0 34.5 40.0 27.0 41.0 56-57 34.83433126265158 38.0 34.0 40.0 26.0 41.0 58-59 34.38060619385748 38.0 34.0 40.0 25.5 41.0 60-61 33.898903323355 37.0 33.0 40.0 23.5 41.0 62-63 33.42289046298817 36.5 32.5 39.0 22.5 41.0 64-65 32.920838295161076 36.0 32.0 39.0 21.5 40.0 66-67 32.35458731886331 35.0 31.0 39.0 19.5 40.0 68-69 31.75992197675764 35.0 31.0 38.0 17.5 40.0 70-71 31.141285035204277 35.0 30.0 37.5 12.0 39.5 72-73 30.538813052144228 34.0 29.5 37.0 8.0 39.0 74-75 29.929855343895674 34.0 29.0 36.0 7.0 39.0 76-77 28.58731051765509 32.5 27.0 35.0 2.0 37.0 78-79 29.26617028244575 34.0 29.0 35.0 2.0 37.0 80-81 28.929710877726944 34.0 29.0 35.0 2.0 37.0 82-83 28.35655689348239 33.5 27.5 35.0 2.0 36.5 84-85 27.748632319787088 33.0 26.0 35.0 2.0 36.0 86-87 27.092458635767407 33.0 25.5 35.0 2.0 35.5 88-89 26.34017868911053 32.0 24.5 35.0 2.0 35.0 90-91 25.772626366169362 32.0 23.5 34.0 2.0 35.0 92-93 24.936889004512985 31.5 19.0 34.0 2.0 35.0 94-95 24.099188399424207 31.0 11.5 34.0 2.0 35.0 96-97 23.045329282141275 31.0 2.0 34.0 2.0 35.0 98-99 21.60219484975231 30.0 2.0 34.0 2.0 35.0 100-101 17.297350801269232 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09301014588651668 1101 2 0.10092265421725699 1101 3 0.16401510174650724 1101 4 0.03793691644006003 1101 5 0.062056323316447504 1101 6 0.05957860100955514 1101 7 0.09425428728646779 1101 8 0.036485186325563745 1101 9 -0.07008699120164863 1101 10-11 0.04993470139374523 1101 12-13 0.10231940336780099 1101 14-15 0.06831114817654083 1101 16-17 0.19921498188917752 1101 18-19 0.09903829445431 1101 20-21 0.12144631570158992 1101 22-23 0.13312961943046275 1101 24-25 0.20385012155872317 1101 26-27 0.17608906364088028 1101 28-29 0.06850349275516265 1101 30-31 0.14677876733937723 1101 32-33 0.174285901057452 1101 34-35 0.11783980663740579 1101 36-37 0.3062270637923703 1101 38-39 0.25812602456488065 1101 40-41 0.16284316326500914 1101 42-43 0.2123628436119347 1101 44-45 0.1708573364291226 1101 46-47 0.1351884834630397 1101 48-49 0.23501002055272835 1101 50-51 0.13479732235059316 1101 52-53 0.10695865043918218 1101 54-55 0.12492108996151785 1101 56-57 0.010790884493893316 1101 58-59 0.37203789865206005 1101 60-61 0.15639635509721472 1101 62-63 0.29015654952673486 1101 64-65 0.1396449235695556 1101 66-67 -0.1410653417733343 1101 68-69 -0.09527055002557816 1101 70-71 -0.09994481730628735 1101 72-73 -0.07793736570600274 1101 74-75 -0.1362988876770359 1101 76-77 -0.21143381069854783 1101 78-79 -0.5323862233066698 1101 80-81 -0.506748687273511 1101 82-83 -0.6004610588082393 1101 84-85 -0.3031385695396871 1101 86-87 -0.4298682721058391 1101 88-89 -0.4350826250521358 1101 90-91 -0.4862729011418878 1101 92-93 -0.9098261406752037 1101 94-95 -0.780358102786952 1101 96-97 -0.7624875280417207 1101 98-99 -0.7285821295621737 1101 100-101 -0.9637058357499129 1102 1 0.09706395491282649 1102 2 0.143236822258217 1102 3 0.08003606585367606 1102 4 0.006899158597946098 1102 5 0.036823412776541886 1102 6 0.03324826953835469 1102 7 0.031511339359994395 1102 8 0.10267322435106507 1102 9 0.14868313753592588 1102 10-11 0.17691044761407682 1102 12-13 0.10545711199434038 1102 14-15 0.16815659400919003 1102 16-17 0.24092820188278807 1102 18-19 0.13674907957221905 1102 20-21 0.09640983573751072 1102 22-23 0.12963833576744577 1102 24-25 0.23002546714702277 1102 26-27 0.21770169391104588 1102 28-29 0.12289490334254793 1102 30-31 0.17762786548559006 1102 32-33 0.07015092950750557 1102 34-35 0.12617062163002402 1102 36-37 0.1359515429275504 1102 38-39 0.10757170179707032 1102 40-41 0.024028481482538666 1102 42-43 0.02845763107836774 1102 44-45 -0.04545978454621036 1102 46-47 -0.12372557923932703 1102 48-49 -0.124397924740272 1102 50-51 -0.10431135772228117 1102 52-53 -0.21281305837731423 1102 54-55 -0.1429519338605445 1102 56-57 -0.18387958702582807 1102 58-59 -0.20356240182196217 1102 60-61 -0.22823716157132168 1102 62-63 -0.21082689849903602 1102 64-65 -0.25150749792296523 1102 66-67 -0.25766091927923185 1102 68-69 -0.3014535801506888 1102 70-71 -0.3276069912531163 1102 72-73 -0.3195046658432865 1102 74-75 -0.4496065195445489 1102 76-77 -0.23568715865264878 1102 78-79 -0.2570358861368227 1102 80-81 -0.4906996616555084 1102 82-83 -0.4786939607138301 1102 84-85 -0.633084087037119 1102 86-87 -0.6088955306257482 1102 88-89 -0.8152850133025673 1102 90-91 -1.231743773293644 1102 92-93 -0.9879839240748751 1102 94-95 -0.9627730332375783 1102 96-97 -1.3018630083252738 1102 98-99 -1.4483110985390901 1102 100-101 -1.7023227918099018 1103 1 0.11492641805466519 1103 2 0.10724840109464395 1103 3 0.0761662182159597 1103 4 0.04506276111654017 1103 5 0.09512590821829292 1103 6 0.04698485930877894 1103 7 0.04242666662893413 1103 8 0.13521421958989777 1103 9 0.15122098743430712 1103 10-11 0.12014286171682187 1103 12-13 0.08268244036705852 1103 14-15 0.19134684063210017 1103 16-17 0.15264891058857444 1103 18-19 0.19171411151940987 1103 20-21 0.16119637998763636 1103 22-23 0.1882910511755469 1103 24-25 0.1478038608988328 1103 26-27 0.24919431148993 1103 28-29 0.30726123342736145 1103 30-31 0.30811903653982853 1103 32-33 0.26705256168193614 1103 34-35 0.20027264352789587 1103 36-37 0.26492212030085227 1103 38-39 0.10133973667300467 1103 40-41 0.15248762981730124 1103 42-43 0.169230911784652 1103 44-45 0.25807200123535523 1103 46-47 0.19523186073570287 1103 48-49 0.20586883996585215 1103 50-51 0.2073929188618635 1103 52-53 0.19638370134108385 1103 54-55 0.1443765766174252 1103 56-57 0.003322321424015229 1103 58-59 0.31999507782254 1103 60-61 0.053789292484715645 1103 62-63 0.08993839851986252 1103 64-65 -0.06862732045605213 1103 66-67 -0.007811962013533957 1103 68-69 0.029852225782629915 1103 70-71 -0.03307552664483637 1103 72-73 -0.35510738859604984 1103 74-75 -0.4301390198744386 1103 76-77 -0.23193515754389438 1103 78-79 -0.3252428843948536 1103 80-81 -0.34583017668633786 1103 82-83 -0.5933733642112209 1103 84-85 -0.638840931472231 1103 86-87 -0.6869310953803378 1103 88-89 -0.7352645229448207 1103 90-91 -0.5727011684538041 1103 92-93 -0.8601108962406876 1103 94-95 -0.6554587473336007 1103 96-97 -0.8984948798360399 1103 98-99 -1.1149182886711877 1103 100-101 -1.7485918702915413 1104 1 0.16265069734638615 1104 2 0.1298742973510798 1104 3 0.11113493181023415 1104 4 0.039169787446759585 1104 5 0.011516942251013518 1104 6 0.07615988302880083 1104 7 0.10107256376611673 1104 8 0.10049451616020377 1104 9 0.1741339259544148 1104 10-11 0.1547162416494885 1104 12-13 0.17394046213743053 1104 14-15 0.22494199494233413 1104 16-17 0.07308936232984564 1104 18-19 0.10930736022131526 1104 20-21 0.23181117887789782 1104 22-23 0.16068595247224238 1104 24-25 0.17649787864981903 1104 26-27 0.20886427293739018 1104 28-29 0.06774127858547274 1104 30-31 0.0268050074993198 1104 32-33 -0.009032854409248614 1104 34-35 0.06769194541868728 1104 36-37 0.0029720344283745703 1104 38-39 0.07961379998243245 1104 40-41 -0.03944556179642689 1104 42-43 -0.10474532415847904 1104 44-45 -0.02836795408693149 1104 46-47 0.03209797573676809 1104 48-49 0.038088032964743945 1104 50-51 0.11615431347892269 1104 52-53 0.21381441286160197 1104 54-55 -0.11746301599874442 1104 56-57 0.017867397468201318 1104 58-59 0.17765454241065015 1104 60-61 -0.2530959186334698 1104 62-63 -0.19141286194395946 1104 64-65 -0.13525615519257173 1104 66-67 -0.3565949348425974 1104 68-69 -0.18150696137132272 1104 70-71 -0.4025313473575167 1104 72-73 -0.650189437845615 1104 74-75 -0.4555104333474098 1104 76-77 -0.42066784793458467 1104 78-79 -0.4947815750497675 1104 80-81 -0.3637314853908826 1104 82-83 -0.2953442276199354 1104 84-85 -0.3967159780115104 1104 86-87 -0.381184445207591 1104 88-89 -0.6179557143103303 1104 90-91 -0.7912043851687116 1104 92-93 -0.8686186768074862 1104 94-95 -1.027684105535922 1104 96-97 -1.1172086742785474 1104 98-99 -1.2288216230854339 1104 100-101 -1.657916340144098 1105 1 0.09084744943500311 1105 2 0.07338880030799544 1105 3 -0.04488872349812567 1105 4 -0.04830604095837998 1105 5 -0.06099596491128523 1105 6 0.08297486472409332 1105 7 0.019730218886742534 1105 8 0.046879572013018844 1105 9 0.1845873193647236 1105 10-11 0.07810214233473545 1105 12-13 0.09058272253356137 1105 14-15 0.13487534704972148 1105 16-17 -0.0068787217779160414 1105 18-19 0.12815463328993815 1105 20-21 0.12116065789789587 1105 22-23 0.04424457445665553 1105 24-25 0.023321308669530083 1105 26-27 0.11357843796132983 1105 28-29 0.08272015777443897 1105 30-31 8.264099211743314E-4 1105 32-33 0.19101220332096602 1105 34-35 0.21607643804988186 1105 36-37 0.19800723578010349 1105 38-39 0.08463585949618846 1105 40-41 0.0703957974451015 1105 42-43 0.040677561413652086 1105 44-45 -0.13874531257749823 1105 46-47 -0.2157129695368667 1105 48-49 -0.29646373648986923 1105 50-51 -0.3567772550933128 1105 52-53 -0.39193232514646326 1105 54-55 -0.2095525682375481 1105 56-57 -0.28807834551499667 1105 58-59 -0.12067832157132585 1105 60-61 -0.0754342270911863 1105 62-63 -0.15446833927428827 1105 64-65 -0.2548722257830107 1105 66-67 -0.22357794432349465 1105 68-69 -0.36472295686555256 1105 70-71 -0.5034043408804685 1105 72-73 -0.4465219263465059 1105 74-75 -0.6101992533981004 1105 76-77 -0.4852214947946223 1105 78-79 -0.490534883976828 1105 80-81 -0.5080504565727075 1105 82-83 -0.5795289643138268 1105 84-85 -0.6149671748574725 1105 86-87 -0.6424451229509529 1105 88-89 -0.8431927367453262 1105 90-91 -1.029360773040672 1105 92-93 -0.9055106321252353 1105 94-95 -0.8384810641391347 1105 96-97 -0.9929807572598683 1105 98-99 -0.9753756454265563 1105 100-101 -1.5099082673007675 1106 1 0.11185851037863159 1106 2 0.07625399875433914 1106 3 0.11526743100018422 1106 4 0.061647625320524924 1106 5 0.05669444696592052 1106 6 0.019134883651915402 1106 7 0.07165987049658895 1106 8 0.02795727464911124 1106 9 0.03345697373487866 1106 10-11 0.08188987474270704 1106 12-13 -0.03574606943789149 1106 14-15 0.030350218399711082 1106 16-17 0.15760204479871476 1106 18-19 0.1465227004836933 1106 20-21 0.19829506512201078 1106 22-23 0.1983150411456691 1106 24-25 0.21426986838087458 1106 26-27 0.24944152674462572 1106 28-29 0.26763784148270986 1106 30-31 0.27599745035966805 1106 32-33 0.2362912900537495 1106 34-35 0.14440073466455772 1106 36-37 0.3147912812068725 1106 38-39 0.1542675557047133 1106 40-41 0.10098255980240367 1106 42-43 -0.033631277483010535 1106 44-45 -0.0485207419037863 1106 46-47 -0.023956625760362726 1106 48-49 -0.1634743242380523 1106 50-51 -0.35488703643758157 1106 52-53 -0.3443326090637342 1106 54-55 -0.45669683370019243 1106 56-57 -0.2971268283320896 1106 58-59 -0.240464397795229 1106 60-61 -0.4291417134375237 1106 62-63 -0.3311015536515498 1106 64-65 -0.5386936733667582 1106 66-67 -0.6480399802469208 1106 68-69 -0.47927371693574017 1106 70-71 -1.1108137929595685 1106 72-73 -0.9333495451875713 1106 74-75 -0.820811647175578 1106 76-77 -0.9916743034067785 1106 78-79 -0.6378587665454916 1106 80-81 -0.7732481142552352 1106 82-83 -1.0392775792313103 1106 84-85 -1.2524050186635414 1106 86-87 -1.0844948479060967 1106 88-89 -1.2818715356175439 1106 90-91 -1.6792002809174065 1106 92-93 -1.4944688134773934 1106 94-95 -1.3744052740013153 1106 96-97 -1.4303543253507236 1106 98-99 -1.569793516441365 1106 100-101 -2.1719883556305835 1107 1 -0.018326253097910694 1107 2 5.657840221289234E-4 1107 3 -0.03362706930261794 1107 4 0.018740495794851597 1107 5 -0.03886159653867338 1107 6 0.05189967765674908 1107 7 0.03087343044290236 1107 8 -0.03476101859960323 1107 9 -0.01328071312697432 1107 10-11 0.07445843059851143 1107 12-13 -0.027915136695760623 1107 14-15 0.054924964912153484 1107 16-17 -0.015289749747985582 1107 18-19 0.08462056102391813 1107 20-21 0.07847008929319799 1107 22-23 0.06995382978858089 1107 24-25 0.09028461545997146 1107 26-27 -0.04914574274372541 1107 28-29 -0.05143773906842597 1107 30-31 -0.050115929320952546 1107 32-33 0.023017325164829572 1107 34-35 0.0313387324758736 1107 36-37 -0.1496027037981449 1107 38-39 -0.13266636930617892 1107 40-41 -0.05809766019964968 1107 42-43 -0.11229670932380742 1107 44-45 -0.08619839568741838 1107 46-47 -0.19064554526411825 1107 48-49 -0.3700678339649599 1107 50-51 -0.1540970902339609 1107 52-53 -0.1691825352220988 1107 54-55 -0.1495005495108046 1107 56-57 -0.21374438528931705 1107 58-59 -0.21691776777196026 1107 60-61 -0.3890655343617979 1107 62-63 -0.32553952490111726 1107 64-65 -0.5446909605275252 1107 66-67 -0.5247327600571623 1107 68-69 -0.2674799493828566 1107 70-71 -0.3572837374632378 1107 72-73 -0.5218226346014809 1107 74-75 -0.613520449028524 1107 76-77 -0.7083986385663827 1107 78-79 -0.6351781555545948 1107 80-81 -0.5023179928857822 1107 82-83 -0.5588679263401524 1107 84-85 -0.9652348712281658 1107 86-87 -0.9979843170608298 1107 88-89 -1.1385190542196177 1107 90-91 -1.3893799799551587 1107 92-93 -1.4549196193606413 1107 94-95 -1.6232579838302215 1107 96-97 -1.7670274140563151 1107 98-99 -1.5638883099857992 1107 100-101 -1.8196181628631702 1108 1 -0.0780804474296275 1108 2 -0.10988449396378286 1108 3 -0.09500196986725484 1108 4 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2316 52-53 0.01092281093782077 2316 54-55 -0.10346301599874153 2316 56-57 -0.09035753778892541 2316 58-59 -0.07866954194007292 2316 60-61 0.11306094411163059 2316 62-63 0.10531743772307323 2316 64-65 0.19960363023103156 2316 66-67 0.13356340773971453 2316 68-69 0.38711900977184754 2316 70-71 0.4492067214549138 2316 72-73 0.6220821122838736 2316 74-75 0.8804451715360564 2316 76-77 0.688892640411698 2316 78-79 0.6027855725639988 2316 80-81 0.6516299648495902 2316 82-83 0.8758389022357811 2316 84-85 1.0808045547298875 2316 86-87 1.288090434555631 2316 88-89 1.2842559024118287 2316 90-91 1.4060667950014718 2316 92-93 1.6112163213427166 2316 94-95 2.0567287690478757 2316 96-97 2.4141061610895598 2316 98-99 2.840849113133583 2316 100-101 2.954007448239178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 11.0 5 34.0 6 57.0 7 171.0 8 408.0 9 794.0 10 1432.0 11 2328.0 12 3549.0 13 5334.0 14 7449.0 15 10204.0 16 12974.0 17 17729.0 18 18444.0 19 15161.0 20 14258.0 21 14936.0 22 16307.0 23 17708.0 24 19779.0 25 22472.0 26 25736.0 27 30272.0 28 35636.0 29 43144.0 30 51254.0 31 61905.0 32 77306.0 33 98808.0 34 132516.0 35 187975.0 36 265549.0 37 331693.0 38 184107.0 39 9985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11976773100266 18.79190420055074 15.08649073042245 23.00183733802415 2 15.90370600628605 19.677293084193924 40.08674978988936 24.332251119630666 3 19.51355694931801 23.44157249560228 29.69019101299515 27.354679542084565 4 13.178595293856274 16.755658210311662 36.30308196411829 33.762664531713774 5 13.909209685681562 36.78135845885542 34.280969949522564 15.028461905940452 6 32.79574812694148 35.727832236578934 16.871656999917693 14.604762636561894 7 28.88201027839379 32.457288951331016 20.01662224284015 18.644078527435045 8 26.36081663099139 35.230406272249084 19.08421111399532 19.32456598276421 9 25.918396712385533 16.962755334027847 20.057498719372866 37.06134923421375 10-11 25.12715612879015 26.452820284661666 26.70275826781035 21.717265318737837 12-13 26.073172949744965 23.66422472887929 27.338412517885736 22.924189803490005 14-15 22.800445024867173 25.296054922468862 25.708704403225223 26.19479564943874 16-17 22.203372075421306 28.353297221632257 26.668813568882467 22.77451713406397 18-19 22.098546647320095 26.769494731013776 29.203202889783825 21.9287557318823 20-21 22.61174688376114 25.846206266608572 29.730533183763846 21.811513665866443 22-23 22.47827395735428 25.8008519475018 29.493775580152537 22.227098514991386 24-25 22.33129826617343 25.91139140690158 29.525559019921477 22.231751307003513 26-27 21.932418876890907 26.308339054083028 29.389599605088375 22.369642463937687 28-29 22.024452619236882 26.52739652303348 29.337099289192142 22.111051568537498 30-31 22.14315973417353 26.12920418977146 29.30529062827002 22.42234544778499 32-33 21.974570500929246 26.156089076343175 29.2620829258831 22.60725749684448 34-35 22.52483561166391 26.316178770930428 29.111046706232496 22.04793891117317 36-37 22.56239224296088 25.99660242469346 29.030332698194535 22.41067263415113 38-39 22.10659789642965 26.342172219713657 28.96428212719213 22.586947756664564 40-41 22.605350312019034 26.16217162025424 28.96421306853828 22.26826499918844 42-43 22.32180090026595 26.09009969347444 28.922014843653542 22.666084562606066 44-45 22.370643783839473 26.403638475427226 28.915392899056137 22.310324841677165 46-47 22.615088908694663 26.643225893900098 28.242716175082887 22.498969022322356 48-49 22.503376411538593 26.607849620831793 28.259319917475406 22.62945405015421 50-51 22.66130526926517 26.891291259487105 28.07746014350525 22.36994332774247 52-53 22.88446129829942 26.726455256698944 27.959411450490652 22.429671994510986 54-55 22.600493292772423 26.876368858663273 28.01258270712397 22.51055514144033 56-57 22.55848955156691 26.98816119609021 27.99488120180079 22.458468050542088 58-59 22.65021994780665 26.706315417992943 27.875081390187585 22.768383244012817 60-61 22.36442036199082 26.913811120234126 28.066418992675402 22.655349525099655 62-63 22.39631307223256 26.934566300683453 28.172148739015903 22.49697188806809 64-65 22.23260063348927 26.89232179228414 28.08037323133642 22.79470434289017 66-67 22.174920294925325 26.893817614756742 27.978675852406088 22.95258623791184 68-69 22.401860605975116 26.70651266787901 28.110488305432785 22.781138420713088 70-71 22.445025734961842 26.739434158966706 27.74744679589507 23.068093310176376 72-73 22.490214926266592 26.885871504714103 27.624471893814682 22.999441675204622 74-75 22.3421261839205 27.208627328624036 27.517498680295265 22.931747807160196 76-77 22.5028935293459 26.903533574295267 27.69619222763908 22.897380668719748 78-79 22.516290735474495 26.990474989687403 27.758831770683557 22.734402504154545 80-81 22.57569656729404 27.110597389903557 27.488155614545228 22.825550428257174 82-83 22.534179336075933 27.154500099320877 27.507736952259766 22.803583612343424 84-85 22.593515620862203 27.138758206508097 27.437715741066963 22.83001043156273 86-87 22.679134434022014 27.140049929412523 27.284017291053008 22.896798345512455 88-89 22.667371321359962 27.206061412038196 27.116626684421202 23.00994058218064 90-91 22.572051922809543 27.15261947194596 27.225449350274005 23.049879254970495 92-93 22.599861772256986 26.942780565329016 27.283974534085864 23.173383128328137 94-95 22.439848982398452 26.975212539400058 27.284138345109817 23.300800133091673 96-97 22.620562494009544 26.695223207591106 27.285849689014434 23.398364609384917 98-99 22.456857572281088 26.787448387098635 27.079986253828693 23.675707786791587 100-101 23.046957072342387 26.26715019643397 26.900113687058386 23.785779044165263 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 784.0 1 529.0 2 414.0 3 571.5 4 453.0 5 390.0 6 765.5 7 1735.5 8 2886.0 9 3289.5 10 2608.0 11 1768.0 12 1623.0 13 1745.5 14 1882.0 15 2035.5 16 2184.5 17 2325.0 18 2492.5 19 2718.0 20 2986.5 21 3565.0 22 4398.5 23 5338.5 24 6539.0 25 8180.0 26 10307.5 27 12848.0 28 15122.5 29 17087.5 30 19311.5 31 22388.0 32 26615.5 33 29134.0 34 30466.0 35 32848.5 36 35928.0 37 39154.0 38 41893.5 39 44372.0 40 47139.0 41 50301.5 42 52325.5 43 54807.0 44 58189.0 45 60850.5 46 62407.0 47 64552.0 48 65481.5 49 64599.5 50 64379.0 51 63203.0 52 62272.5 53 62338.5 54 63123.0 55 60069.5 56 54414.5 57 50218.5 58 46493.5 59 41252.0 60 33531.5 61 27420.5 62 22506.0 63 17001.5 64 12548.0 65 9401.5 66 6807.5 67 5100.0 68 3881.5 69 2914.0 70 2170.5 71 1429.5 72 935.5 73 690.0 74 489.5 75 339.5 76 231.0 77 129.5 78 78.5 79 49.5 80 28.5 81 16.0 82 6.5 83 4.5 84 4.0 85 2.0 86 2.0 87 1.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007770092740373575 2 0.014446616872842719 3 0.009611892501054719 4 0.0 5 0.0012086810929470004 6 0.0018417997606811436 7 0.0 8 0.0 9 5.755624252128574E-5 10-11 0.0 12-13 7.482311527767146E-4 14-15 0.0 16-17 0.002532474670936572 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003453374551277144 26-27 0.019195006880848794 28-29 0.006446299162384003 30-31 0.003280705823713287 32-33 0.0 34-35 0.009065108197102503 36-37 8.921217590799289E-4 38-39 0.002964146489846215 40-41 0.0021295809732875723 42-43 0.0031943714599313583 44-45 0.0 46-47 1.4389060630321435E-4 48-49 5.467843039522146E-4 50-51 0.004201605704053858 52-53 0.00823054268054386 54-55 0.0072520865576820035 56-57 0.017525875847731506 58-59 0.024634071799110292 60-61 0.0038274901276655012 62-63 0.02158359094548215 64-65 0.02215915337069501 66-67 0.014130057538975647 68-69 0.03323873005604251 70-71 0.01726687275638572 72-73 0.0052376180694370025 74-75 0.01726687275638572 76-77 0.02103680664152994 78-79 0.027483105803913936 80-81 0.012403370263337076 82-83 0.036605770243537734 84-85 0.022734715795907866 86-87 0.03332506441982444 88-89 0.023598059433727153 90-91 0.029152236837031227 92-93 0.025468637315668938 94-95 0.01625963851226322 96-97 0.016835200937476077 98-99 0.01266237335468286 100-101 0.01185658595938486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1737431.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.72663541230221 #Duplication Level Percentage of deduplicated Percentage of total 1 80.83514198005008 49.89681337504237 2 11.51439636120638 14.21489892361851 3 3.2681623747421535 6.051980021217382 4 1.4059610237880429 3.4714097407708673 5 0.7481668473788039 2.309091110786149 6 0.4720313713827545 1.7482145018707387 7 0.312012020600506 1.3481616567904213 8 0.22521796835692273 1.1121557936853748 9 0.17542374231755567 0.9745485646227671 >10 0.9559322444800092 10.643129524445778 >50 0.05745240219342411 2.4181169555882143 >100 0.02628127373196909 2.998494641556213 >500 0.002609046673146162 1.0279442884611945 >1k 0.001024982621593135 0.9446021178042859 >5k 1.863604766532973E-4 0.8404387837396471 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7708 0.4436435173540704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6904 0.3973682983669567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4119 0.23707416294517594 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1819 0.10469480514621876 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.877812126064287E-5 0.0 18-19 0.0 0.0 0.0 1.1511248504257147E-4 0.0 20-21 0.0 0.0 0.0 1.1511248504257147E-4 0.0 22-23 0.0 0.0 0.0 1.4389060630321435E-4 0.0 24-25 0.0 0.0 0.0 4.31671818909643E-4 0.0 26-27 0.0 2.877812126064287E-5 0.0 8.345655165586432E-4 0.0 28-29 0.0 5.755624252128574E-5 0.0 0.0029641464898462156 0.0 30-31 0.0 5.755624252128574E-5 0.0 0.009813339349879218 0.0 32-33 0.0 5.755624252128574E-5 0.0 0.02477796240541351 0.0 34-35 0.0 5.755624252128574E-5 0.0 0.04759901256510331 0.0 36-37 0.0 5.755624252128574E-5 0.0 0.08460767650629003 0.0 38-39 0.0 5.755624252128574E-5 0.0 0.14046600987319785 0.0 40-41 0.0 5.755624252128574E-5 0.0 0.1977632493031378 0.0 42-43 0.0 5.755624252128574E-5 0.0 0.2581109695867059 0.0 44-45 0.0 5.755624252128574E-5 0.0 0.331754181892691 2.877812126064287E-5 46-47 0.0 5.755624252128574E-5 0.0 0.41463517112334247 5.755624252128574E-5 48-49 0.0 5.755624252128574E-5 0.0 0.5034444533336864 5.755624252128574E-5 50-51 0.0 5.755624252128574E-5 0.0 0.5993331533741484 5.755624252128574E-5 52-53 0.0 5.755624252128574E-5 0.0 0.6883438824333168 1.1511248504257147E-4 54-55 0.0 5.755624252128574E-5 0.0 0.7849232573840342 1.726687275638572E-4 56-57 0.0 5.755624252128574E-5 0.0 0.8981076083021426 1.726687275638572E-4 58-59 0.0 5.755624252128574E-5 0.0 1.01840015517163 2.014468488245001E-4 60-61 0.0 5.755624252128574E-5 0.0 1.1453116699310648 2.3022497008514295E-4 62-63 0.0 5.755624252128574E-5 0.0 1.2702662724447764 2.3022497008514295E-4 64-65 0.0 5.755624252128574E-5 0.0 1.3972641215679933 2.3022497008514295E-4 66-67 0.0 5.755624252128574E-5 0.0 1.5481190332162833 2.3022497008514295E-4 68-69 0.0 5.755624252128574E-5 0.0 1.7094204028821864 2.3022497008514295E-4 70-71 0.0 5.755624252128574E-5 0.0 1.8770817373466917 2.3022497008514295E-4 72-73 0.0 5.755624252128574E-5 0.0 2.049002233757772 2.3022497008514295E-4 74-75 0.0 5.755624252128574E-5 0.0 2.2221601893830605 2.3022497008514295E-4 76-77 0.0 5.755624252128574E-5 0.0 2.4083834120606804 2.3022497008514295E-4 78-79 0.0 5.755624252128574E-5 0.0 2.6132836354364573 2.3022497008514295E-4 80-81 0.0 5.755624252128574E-5 0.0 2.8367457470253497 3.165593338670716E-4 82-83 0.0 5.755624252128574E-5 0.0 3.0641504612269497 4.028936976490002E-4 84-85 0.0 5.755624252128574E-5 0.0 3.2921882940962837 4.028936976490002E-4 86-87 0.0 5.755624252128574E-5 0.0 3.5226434891515117 4.892280614309288E-4 88-89 0.0 5.755624252128574E-5 0.0 3.7719771317537214 5.755624252128574E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3615 0.0 56.76792 1 GTATCAA 5975 0.0 39.35454 1 TATCAAC 7825 0.0 30.049406 2 ATCAACG 7730 0.0 30.044806 3 TCAACGC 7840 0.0 29.681276 4 CAACGCA 8020 0.0 29.015114 5 AACGCAG 8355 0.0 27.851728 6 TGGTATC 1550 0.0 27.582008 2 GTGGTAT 1820 0.0 25.056904 1 ACGCAGA 9490 0.0 24.370548 7 CGCAGAG 9660 0.0 23.941668 8 GTACATG 5985 0.0 22.700188 1 TACATGG 5785 0.0 22.498951 2 GAGTACT 6875 0.0 22.002108 12-13 ACATGGG 5740 0.0 21.843998 3 GCAGAGT 10745 0.0 21.347315 9 GTACTTT 7235 0.0 20.710394 14-15 CAGAGTA 10220 0.0 20.470222 10-11 TACCGTG 190 8.27021E-6 19.995808 7 AGAGTAC 9810 0.0 19.776554 10-11 >>END_MODULE